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Protein

Alpha-lactalbumin

Gene

Lalba

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi98 – 10912Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Milk protein

Keywords - Biological processi

Lactose biosynthesis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5653890. Lactose synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-lactalbumin
Alternative name(s):
Lactose synthase B protein
Gene namesi
Name:Lalba
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:96742. Lalba.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 143123Alpha-lactalbuminPRO_0000018446Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 140
Disulfide bondi48 ↔ 131
Glycosylationi65 – 651N-linked (GlcNAc...)Sequence analysis
Disulfide bondi81 ↔ 97
Disulfide bondi93 ↔ 111

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP29752.
PRIDEiP29752.

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.

Gene expression databases

BgeeiP29752.
CleanExiMM_LALBA.
ExpressionAtlasiP29752. baseline and differential.
GenevisibleiP29752. MM.

Interactioni

Subunit structurei

Lactose synthase (LS) is heterodimer of a catalytic component, beta1,4-galactosyltransferase (beta4Gal-T1) and a regulatory component, alpha-lactalbumin (LA).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
B4GALT1P0803710EBI-1031454,EBI-1031436From a different organism.

Protein-protein interaction databases

IntActiP29752. 1 interaction.
STRINGi10090.ENSMUSP00000023726.

Structurei

Secondary structure

1
143
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 317Combined sources
Helixi33 – 353Combined sources
Helixi38 – 403Combined sources
Helixi43 – 5412Combined sources
Beta strandi61 – 644Combined sources
Beta strandi67 – 704Combined sources
Turni71 – 744Combined sources
Turni77 – 804Combined sources
Beta strandi81 – 833Combined sources
Beta strandi84 – 863Combined sources
Helixi97 – 1004Combined sources
Beta strandi101 – 1033Combined sources
Helixi106 – 11813Combined sources
Helixi121 – 1233Combined sources
Helixi127 – 1304Combined sources
Beta strandi131 – 1333Combined sources
Helixi136 – 1383Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NF5X-ray2.00A/C21-143[»]
1NHEX-ray2.50A/C21-143[»]
1NKHX-ray2.00A/C21-143[»]
1NMMX-ray2.00A/C21-143[»]
1NQIX-ray2.00A/C21-143[»]
1NWGX-ray2.32A/C21-143[»]
1O23X-ray2.32A/C21-143[»]
1OQMX-ray2.10A/C21-143[»]
1PZYX-ray2.30A/C21-143[»]
1YROX-ray1.90A/C21-143[»]
2FYCX-ray2.00A/C21-143[»]
2FYDX-ray2.00A/C21-143[»]
ProteinModelPortaliP29752.
SMRiP29752. Positions 21-143.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29752.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 22 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IX41. Eukaryota.
ENOG410ZQK6. LUCA.
GeneTreeiENSGT00550000074398.
HOGENOMiHOG000037357.
HOVERGENiHBG052297.
InParanoidiP29752.
KOiK00704.
OMAiGIDYWKA.
OrthoDBiEOG7BW0M5.
PhylomeDBiP29752.
TreeFamiTF324882.

Family and domain databases

InterProiIPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000545. Lactalbumin.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00062. Lys. 1 hit.
[Graphical view]
PRINTSiPR00136. LACTALBUMIN.
PR00135. LYZLACT.
SMARTiSM00263. LYZ1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29752-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMHFVPLFLV CILSLPAFQA TELTKCKVSH AIKDIDGYQG ISLLEWACVL
60 70 80 90 100
FHTSGYDTQA VVNDNGSTEY GLFQISDRFW CKSSEFPESE NICGISCDKL
110 120 130 140
LDDELDDDIA CAKKILAIKG IDYWKAYKPM CSEKLEQWRC EKP
Length:143
Mass (Da):16,260
Last modified:April 1, 1993 - v1
Checksum:i0322892711FF4309
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80909 mRNA. Translation: AAA37208.1.
M87863 Genomic DNA. Translation: AAA37209.1.
BC006922 mRNA. Translation: AAH06922.1.
BC069916 mRNA. Translation: AAH69916.1.
CCDSiCCDS27795.1.
PIRiJC1302.
RefSeqiNP_034809.1. NM_010679.1.
XP_006520580.1. XM_006520517.1.
UniGeneiMm.448567.
Mm.5074.

Genome annotation databases

EnsembliENSMUST00000023726; ENSMUSP00000023726; ENSMUSG00000022991.
GeneIDi16770.
KEGGimmu:16770.
UCSCiuc007xmo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80909 mRNA. Translation: AAA37208.1.
M87863 Genomic DNA. Translation: AAA37209.1.
BC006922 mRNA. Translation: AAH06922.1.
BC069916 mRNA. Translation: AAH69916.1.
CCDSiCCDS27795.1.
PIRiJC1302.
RefSeqiNP_034809.1. NM_010679.1.
XP_006520580.1. XM_006520517.1.
UniGeneiMm.448567.
Mm.5074.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NF5X-ray2.00A/C21-143[»]
1NHEX-ray2.50A/C21-143[»]
1NKHX-ray2.00A/C21-143[»]
1NMMX-ray2.00A/C21-143[»]
1NQIX-ray2.00A/C21-143[»]
1NWGX-ray2.32A/C21-143[»]
1O23X-ray2.32A/C21-143[»]
1OQMX-ray2.10A/C21-143[»]
1PZYX-ray2.30A/C21-143[»]
1YROX-ray1.90A/C21-143[»]
2FYCX-ray2.00A/C21-143[»]
2FYDX-ray2.00A/C21-143[»]
ProteinModelPortaliP29752.
SMRiP29752. Positions 21-143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP29752. 1 interaction.
STRINGi10090.ENSMUSP00000023726.

Proteomic databases

PaxDbiP29752.
PRIDEiP29752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023726; ENSMUSP00000023726; ENSMUSG00000022991.
GeneIDi16770.
KEGGimmu:16770.
UCSCiuc007xmo.1. mouse.

Organism-specific databases

CTDi3906.
MGIiMGI:96742. Lalba.

Phylogenomic databases

eggNOGiENOG410IX41. Eukaryota.
ENOG410ZQK6. LUCA.
GeneTreeiENSGT00550000074398.
HOGENOMiHOG000037357.
HOVERGENiHBG052297.
InParanoidiP29752.
KOiK00704.
OMAiGIDYWKA.
OrthoDBiEOG7BW0M5.
PhylomeDBiP29752.
TreeFamiTF324882.

Enzyme and pathway databases

ReactomeiR-MMU-5653890. Lactose synthesis.

Miscellaneous databases

ChiTaRSiLalba. mouse.
EvolutionaryTraceiP29752.
NextBioi290598.
PROiP29752.
SOURCEiSearch...

Gene expression databases

BgeeiP29752.
CleanExiMM_LALBA.
ExpressionAtlasiP29752. baseline and differential.
GenevisibleiP29752. MM.

Family and domain databases

InterProiIPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000545. Lactalbumin.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00062. Lys. 1 hit.
[Graphical view]
PRINTSiPR00136. LACTALBUMIN.
PR00135. LYZLACT.
SMARTiSM00263. LYZ1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the murine alpha-lactalbumin-encoding cDNA: interspecies comparison of the coding frame and deduced pre-protein."
    Vilotte J.-L., Soulier S., Mercier J.-C.
    Gene 112:251-255(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. "Isolation and characterization of the mouse alpha-lactalbumin-encoding gene: interspecies comparison, tissue- and stage-specific expression."
    Vilotte J.-L., Soulier S.
    Gene 119:287-292(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "Crystal structure of lactose synthase reveals a large conformational change in its catalytic component, the beta1,4-galactosyltransferase-I."
    Ramakrishnan B., Qasba P.K.
    J. Mol. Biol. 310:205-218(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 21-143 IN COMPLEX WITH B4GALT1.
  5. "The role of tryptophan 314 in the conformational changes of beta1,4-galactosyltransferase-I."
    Ramasamy V., Ramakrishnan B., Boeggeman E., Qasba P.K.
    J. Mol. Biol. 331:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 21-143 IN COMPLEX WITH B4GALT1.
  6. "Alpha-lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc."
    Ramakrishnan B., Shah P.S., Qasba P.K.
    J. Biol. Chem. 276:37665-37671(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.32 ANGSTROMS) OF 21-143 IN COMPLEX WITH B4GALT1 AND UDP-GLC.

Entry informationi

Entry nameiLALBA_MOUSE
AccessioniPrimary (citable) accession number: P29752
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: December 9, 2015
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.