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P29749

- MTT8_THET8

UniProt

P29749 - MTT8_THET8

Protein

Modification methylase TthHB8I

Gene

tthHB8IM

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands and protects the DNA from cleavage by the TthHB8I endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-1622-MONOMER.

    Protein family/group databases

    REBASEi3523. M.TthHB8I.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase TthHB8I (EC:2.1.1.72)
    Short name:
    M.TthHB8I
    Alternative name(s):
    Adenine-specific methyltransferase TthHB8I
    Gene namesi
    Name:tthHB8IM
    Ordered Locus Names:TTHA1583
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Modification methylase TthHB8IPRO_0000087981Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi300852.TTHA1583.

    Structurei

    3D structure databases

    ProteinModelPortaliP29749.
    SMRiP29749. Positions 19-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0827.
    HOGENOMiHOG000043650.
    OMAiYPWREEF.
    OrthoDBiEOG644ZKQ.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    3.90.220.10. 1 hit.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR021188. N6_DNA_MeTrfase_TaqI.
    IPR023135. N6_DNA_MeTrfase_TaqI_C.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view]
    PfamiPF12950. TaqI_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037236. Ade-sp_methyltransferase_TaqI. 1 hit.
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29749-1 [UniParc]FASTAAdd to Basket

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    MLFPPSLPPT ASGRSLGRVE TPPGLVRFMV GLAEAPKGGR VLEPACADGP    50
    FLRAFREAHG TGYRFVGVEI DPHALDLPPW AEGVVADFLL WEPGEAFDLI 100
    LGNPPYGIVG EASKYPIHVL REVKGLYKKT LSTWKGKYNL YGAFIEKSVR 150
    LLREGGTLVF VVPATWLVLD DFSLLRSFLA REGRTEVYYL GEVFPGRKVS 200
    AVVLRFRKGG KGLALWDTRR DGETFTPLLW SEKPEWKGEI IRFETGWTRE 250
    MEASGPPLGS LFHIRFAARS PEFKKHPAVQ KEPEPGLVPV LTGRNLKPGW 300
    IDYESNHSGL WMPKERAKEL RDFYATPHLV VAHTKGTKVV AAWDEKAYPW 350
    REEFHLLPKE GVELDPLFLV EWLNSDKIQE YVKTLYRDFV PHLTLRMLER 400
    IPALLPRKGN TERRKHGPYT SPESAGSF 428
    Length:428
    Mass (Da):48,408
    Last modified:March 29, 2005 - v2
    Checksum:i6F1407F702D79B57
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 361P → R in AAA27488. (PubMed:1339363)Curated
    Sequence conflicti39 – 391G → V in AAA27488. (PubMed:1339363)Curated
    Sequence conflicti56 – 561R → P in AAA27488. (PubMed:1339363)Curated
    Sequence conflicti235 – 2417EWKGEII → GMEGRDH in AAA27488. (PubMed:1339363)Curated
    Sequence conflicti265 – 2651R → P in AAA27488. (PubMed:1339363)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74795 Genomic DNA. Translation: AAA27488.1.
    AP008226 Genomic DNA. Translation: BAD71406.1.
    PIRiJH0634.
    RefSeqiYP_144849.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD71406; BAD71406; BAD71406.
    GeneIDi3169149.
    KEGGittj:TTHA1583.
    PATRICi23958123. VBITheThe93045_1554.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74795 Genomic DNA. Translation: AAA27488.1 .
    AP008226 Genomic DNA. Translation: BAD71406.1 .
    PIRi JH0634.
    RefSeqi YP_144849.1. NC_006461.1.

    3D structure databases

    ProteinModelPortali P29749.
    SMRi P29749. Positions 19-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 300852.TTHA1583.

    Protein family/group databases

    REBASEi 3523. M.TthHB8I.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD71406 ; BAD71406 ; BAD71406 .
    GeneIDi 3169149.
    KEGGi ttj:TTHA1583.
    PATRICi 23958123. VBITheThe93045_1554.

    Phylogenomic databases

    eggNOGi COG0827.
    HOGENOMi HOG000043650.
    OMAi YPWREEF.
    OrthoDBi EOG644ZKQ.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-1622-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    3.90.220.10. 1 hit.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR021188. N6_DNA_MeTrfase_TaqI.
    IPR023135. N6_DNA_MeTrfase_TaqI_C.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view ]
    Pfami PF12950. TaqI_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037236. Ade-sp_methyltransferase_TaqI. 1 hit.
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of genes encoding the TthHB8I restriction and modification enzymes: comparison with the isoschizomeric TaqI enzymes."
      Barany F., Danzitz M., Zebala J., Mayer A.
      Gene 112:3-12(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.

    Entry informationi

    Entry nameiMTT8_THET8
    AccessioniPrimary (citable) accession number: P29749
    Secondary accession number(s): Q5SHZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 93 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3