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P29749 (MTT8_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase TthHB8I

Short name=M.TthHB8I
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase TthHB8I
Gene names
Name:tthHB8IM
Ordered Locus Names:TTHA1583
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands and protects the DNA from cleavage by the TthHB8I endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Modification methylase TthHB8I
PRO_0000087981

Experimental info

Sequence conflict361P → R in AAA27488. Ref.1
Sequence conflict391G → V in AAA27488. Ref.1
Sequence conflict561R → P in AAA27488. Ref.1
Sequence conflict235 – 2417EWKGEII → GMEGRDH in AAA27488. Ref.1
Sequence conflict2651R → P in AAA27488. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P29749 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: 6F1407F702D79B57

FASTA42848,408
        10         20         30         40         50         60 
MLFPPSLPPT ASGRSLGRVE TPPGLVRFMV GLAEAPKGGR VLEPACADGP FLRAFREAHG 

        70         80         90        100        110        120 
TGYRFVGVEI DPHALDLPPW AEGVVADFLL WEPGEAFDLI LGNPPYGIVG EASKYPIHVL 

       130        140        150        160        170        180 
REVKGLYKKT LSTWKGKYNL YGAFIEKSVR LLREGGTLVF VVPATWLVLD DFSLLRSFLA 

       190        200        210        220        230        240 
REGRTEVYYL GEVFPGRKVS AVVLRFRKGG KGLALWDTRR DGETFTPLLW SEKPEWKGEI 

       250        260        270        280        290        300 
IRFETGWTRE MEASGPPLGS LFHIRFAARS PEFKKHPAVQ KEPEPGLVPV LTGRNLKPGW 

       310        320        330        340        350        360 
IDYESNHSGL WMPKERAKEL RDFYATPHLV VAHTKGTKVV AAWDEKAYPW REEFHLLPKE 

       370        380        390        400        410        420 
GVELDPLFLV EWLNSDKIQE YVKTLYRDFV PHLTLRMLER IPALLPRKGN TERRKHGPYT 


SPESAGSF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of genes encoding the TthHB8I restriction and modification enzymes: comparison with the isoschizomeric TaqI enzymes."
Barany F., Danzitz M., Zebala J., Mayer A.
Gene 112:3-12(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74795 Genomic DNA. Translation: AAA27488.1.
AP008226 Genomic DNA. Translation: BAD71406.1.
PIRJH0634.
RefSeqYP_144849.1. NC_006461.1.

3D structure databases

ProteinModelPortalP29749.
SMRP29749. Positions 19-402.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1583.

Protein family/group databases

REBASE3523. M.TthHB8I.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71406; BAD71406; BAD71406.
GeneID3169149.
KEGGttj:TTHA1583.
PATRIC23958123. VBITheThe93045_1554.

Phylogenomic databases

eggNOGCOG0827.
HOGENOMHOG000043650.
OMAYPWREEF.
OrthoDBEOG644ZKQ.
ProtClustDBCLSK738767.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1622-MONOMER.

Family and domain databases

Gene3D3.90.220.10. 1 hit.
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR021188. N6_DNA_MeTrfase_TaqI.
IPR023135. N6_DNA_MeTrfase_TaqI_C.
IPR025931. TaqI_C.
[Graphical view]
PfamPF12950. TaqI_C. 1 hit.
[Graphical view]
PIRSFPIRSF037236. Ade-sp_methyltransferase_TaqI. 1 hit.
PRINTSPR00507. N12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTT8_THET8
AccessionPrimary (citable) accession number: P29749
Secondary accession number(s): Q5SHZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 29, 2005
Last modified: April 16, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries