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Protein

Modification methylase TthHB8I

Gene

tthHB8IM

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands and protects the DNA from cleavage by the TthHB8I endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1622-MONOMER.

Protein family/group databases

REBASEi3523. M.TthHB8I.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase TthHB8I (EC:2.1.1.72)
Short name:
M.TthHB8I
Alternative name(s):
Adenine-specific methyltransferase TthHB8I
Gene namesi
Name:tthHB8IM
Ordered Locus Names:TTHA1583
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Modification methylase TthHB8IPRO_0000087981Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1583.

Structurei

3D structure databases

ProteinModelPortaliP29749.
SMRiP29749. Positions 19-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0827.
HOGENOMiHOG000043650.
OMAiYPWREEF.
OrthoDBiEOG644ZKQ.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.90.220.10. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR021188. N6_DNA_MeTrfase_TaqI.
IPR023135. N6_DNA_MeTrfase_TaqI_C.
IPR029063. SAM-dependent_MTases.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF12950. TaqI_C. 1 hit.
[Graphical view]
PIRSFiPIRSF037236. Ade-sp_methyltransferase_TaqI. 1 hit.
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFPPSLPPT ASGRSLGRVE TPPGLVRFMV GLAEAPKGGR VLEPACADGP
60 70 80 90 100
FLRAFREAHG TGYRFVGVEI DPHALDLPPW AEGVVADFLL WEPGEAFDLI
110 120 130 140 150
LGNPPYGIVG EASKYPIHVL REVKGLYKKT LSTWKGKYNL YGAFIEKSVR
160 170 180 190 200
LLREGGTLVF VVPATWLVLD DFSLLRSFLA REGRTEVYYL GEVFPGRKVS
210 220 230 240 250
AVVLRFRKGG KGLALWDTRR DGETFTPLLW SEKPEWKGEI IRFETGWTRE
260 270 280 290 300
MEASGPPLGS LFHIRFAARS PEFKKHPAVQ KEPEPGLVPV LTGRNLKPGW
310 320 330 340 350
IDYESNHSGL WMPKERAKEL RDFYATPHLV VAHTKGTKVV AAWDEKAYPW
360 370 380 390 400
REEFHLLPKE GVELDPLFLV EWLNSDKIQE YVKTLYRDFV PHLTLRMLER
410 420
IPALLPRKGN TERRKHGPYT SPESAGSF
Length:428
Mass (Da):48,408
Last modified:March 28, 2005 - v2
Checksum:i6F1407F702D79B57
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361P → R in AAA27488 (PubMed:1339363).Curated
Sequence conflicti39 – 391G → V in AAA27488 (PubMed:1339363).Curated
Sequence conflicti56 – 561R → P in AAA27488 (PubMed:1339363).Curated
Sequence conflicti235 – 2417EWKGEII → GMEGRDH in AAA27488 (PubMed:1339363).Curated
Sequence conflicti265 – 2651R → P in AAA27488 (PubMed:1339363).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74795 Genomic DNA. Translation: AAA27488.1.
AP008226 Genomic DNA. Translation: BAD71406.1.
PIRiJH0634.
RefSeqiWP_011228786.1. NC_006461.1.
YP_144849.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71406; BAD71406; BAD71406.
GeneIDi3169149.
KEGGittj:TTHA1583.
PATRICi23958123. VBITheThe93045_1554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74795 Genomic DNA. Translation: AAA27488.1.
AP008226 Genomic DNA. Translation: BAD71406.1.
PIRiJH0634.
RefSeqiWP_011228786.1. NC_006461.1.
YP_144849.1. NC_006461.1.

3D structure databases

ProteinModelPortaliP29749.
SMRiP29749. Positions 19-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1583.

Protein family/group databases

REBASEi3523. M.TthHB8I.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71406; BAD71406; BAD71406.
GeneIDi3169149.
KEGGittj:TTHA1583.
PATRICi23958123. VBITheThe93045_1554.

Phylogenomic databases

eggNOGiCOG0827.
HOGENOMiHOG000043650.
OMAiYPWREEF.
OrthoDBiEOG644ZKQ.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1622-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.90.220.10. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR021188. N6_DNA_MeTrfase_TaqI.
IPR023135. N6_DNA_MeTrfase_TaqI_C.
IPR029063. SAM-dependent_MTases.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF12950. TaqI_C. 1 hit.
[Graphical view]
PIRSFiPIRSF037236. Ade-sp_methyltransferase_TaqI. 1 hit.
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of genes encoding the TthHB8I restriction and modification enzymes: comparison with the isoschizomeric TaqI enzymes."
    Barany F., Danzitz M., Zebala J., Mayer A.
    Gene 112:3-12(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiMTT8_THET8
AccessioniPrimary (citable) accession number: P29749
Secondary accession number(s): Q5SHZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 31, 1993
Last sequence update: March 28, 2005
Last modified: March 3, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.