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P29748

- T2T8_THET8

UniProt

P29748 - T2T8_THET8

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Protein

Type-2 restriction enzyme TthHB8I

Gene

tthHB8IR

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Recognizes the double-stranded sequence TCGA and cleaves after T-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1623-MONOMER.

Protein family/group databases

REBASEi1831. TthHB8I.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme TthHB8I (EC:3.1.21.4)
Short name:
R.TthHB8I
Alternative name(s):
Endonuclease TthHB8I
Type II restriction enzyme TthHB8I
Gene namesi
Name:tthHB8IR
Ordered Locus Names:TTHA1584
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Type-2 restriction enzyme TthHB8IPRO_0000077370Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1584.

Structurei

3D structure databases

ProteinModelPortaliP29748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG273292.
OMAiARVNEIN.
OrthoDBiEOG61KBSJ.

Family and domain databases

InterProiIPR019073. Restrct_endonuc_II_TaqI.
[Graphical view]
PfamiPF09573. RE_TaqI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29748-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPTQAQKVL EAFEDFLKCL DLESYQEKYR PIKTVEQDLP RELNPLPDLY
60 70 80 90 100
DHYWKPNGNT LHFPDFETFF DQWWEKRLRP LNEFIRKYFW GCSYEFVRLG
110 120 130 140 150
LEARLYRTAV SIWTQFHFCY RWNASCQLRL TATWELDAQG IDAQIQAEDR
160 170 180 190 200
LIGIQIKKET YRSEAREGNR FLRRREHSAL LEVPYTLQSP EELERKAQRA
210 220 230 240 250
RTREEAYRLW VKIAHHLERL PNGFVIFRES YVKDLENFLK QNATTLSGLI
260
PWDKVAREAL TGP
Length:263
Mass (Da):31,584
Last modified:March 29, 2005 - v2
Checksum:i10EF1995DB291D98
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191C → S in AAA27490. (PubMed:1339363)Curated
Sequence conflicti119 – 1191C → S in AAA27489. (PubMed:1339363)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76680 Genomic DNA. Translation: AAA27490.1.
M74795 Genomic DNA. Translation: AAA27489.1.
AP008226 Genomic DNA. Translation: BAD71407.1.
RefSeqiYP_144850.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71407; BAD71407; BAD71407.
GeneIDi3170138.
KEGGittj:TTHA1584.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76680 Genomic DNA. Translation: AAA27490.1 .
M74795 Genomic DNA. Translation: AAA27489.1 .
AP008226 Genomic DNA. Translation: BAD71407.1 .
RefSeqi YP_144850.1. NC_006461.1.

3D structure databases

ProteinModelPortali P29748.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 300852.TTHA1584.

Protein family/group databases

REBASEi 1831. TthHB8I.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD71407 ; BAD71407 ; BAD71407 .
GeneIDi 3170138.
KEGGi ttj:TTHA1584.

Phylogenomic databases

eggNOGi NOG273292.
OMAi ARVNEIN.
OrthoDBi EOG61KBSJ.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-1623-MONOMER.

Family and domain databases

InterProi IPR019073. Restrct_endonuc_II_TaqI.
[Graphical view ]
Pfami PF09573. RE_TaqI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of genes encoding the TthHB8I restriction and modification enzymes: comparison with the isoschizomeric TaqI enzymes."
    Barany F., Danzitz M., Zebala J., Mayer A.
    Gene 112:3-12(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiT2T8_THET8
AccessioniPrimary (citable) accession number: P29748
Secondary accession number(s): Q5SHZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 29, 2005
Last modified: October 29, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3