P29748 (T2T8_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme TthHB8I
Short name=R.TthHB8I
EC=3.1.21.4

Alternative name(s):
Endonuclease TthHB8I
Type II restriction enzyme TthHB8I

Gene names

Name:	tthHB8IR
Ordered Locus Names:	TTHA1584

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence TCGA and cleaves after T-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Type-2 restriction enzyme TthHB8I		PRO_0000077370
Experimental info
	Sequence conflict	119	1	C → S in AAA27490. Ref.1
	Sequence conflict	119	1	C → S in AAA27489. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29748 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 10EF1995DB291D98	FASTA	263	31,584
	10 20 30 40 50 60 MAPTQAQKVL EAFEDFLKCL DLESYQEKYR PIKTVEQDLP RELNPLPDLY DHYWKPNGNT 70 80 90 100 110 120 LHFPDFETFF DQWWEKRLRP LNEFIRKYFW GCSYEFVRLG LEARLYRTAV SIWTQFHFCY 130 140 150 160 170 180 RWNASCQLRL TATWELDAQG IDAQIQAEDR LIGIQIKKET YRSEAREGNR FLRRREHSAL 190 200 210 220 230 240 LEVPYTLQSP EELERKAQRA RTREEAYRLW VKIAHHLERL PNGFVIFRES YVKDLENFLK 250 260 QNATTLSGLI PWDKVAREAL TGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of genes encoding the TthHB8I restriction and modification enzymes: comparison with the isoschizomeric TaqI enzymes." Barany F., Danzitz M., Zebala J., Mayer A. Gene 112:3-12(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M76680 Genomic DNA. Translation: AAA27490.1. M74795 Genomic DNA. Translation: AAA27489.1. AP008226 Genomic DNA. Translation: BAD71407.1.
RefSeq	YP_144850.1. NC_006461.1.
3D structure databases
ProteinModelPortal	P29748.
ModBase	Search...
Protein-protein interaction databases
STRING	300852.TTHA1584.
Protein family/group databases
REBASE	1831. TthHB8I.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD71407; BAD71407; BAD71407.
GeneID	3170138.
KEGG	ttj:TTHA1584.
Phylogenomic databases
eggNOG	NOG273292.
OMA	ARVNEIN.
ProtClustDB	CLSK2395607.
Family and domain databases
InterPro	IPR019073. Restrct_endonuc_II_TaqI. [Graphical view]
Pfam	PF09573. RE_TaqI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2T8_THET8

Accession

Primary (citable) accession number: P29748
Secondary accession number(s): Q5SHZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program