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P29748

- T2T8_THET8

UniProt

P29748 - T2T8_THET8

Protein

Type-2 restriction enzyme TthHB8I

Gene

tthHB8IR

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence TCGA and cleaves after T-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-1623-MONOMER.

    Protein family/group databases

    REBASEi1831. TthHB8I.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme TthHB8I (EC:3.1.21.4)
    Short name:
    R.TthHB8I
    Alternative name(s):
    Endonuclease TthHB8I
    Type II restriction enzyme TthHB8I
    Gene namesi
    Name:tthHB8IR
    Ordered Locus Names:TTHA1584
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 263263Type-2 restriction enzyme TthHB8IPRO_0000077370Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi300852.TTHA1584.

    Structurei

    3D structure databases

    ProteinModelPortaliP29748.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG273292.
    OMAiARVNEIN.
    OrthoDBiEOG61KBSJ.

    Family and domain databases

    InterProiIPR019073. Restrct_endonuc_II_TaqI.
    [Graphical view]
    PfamiPF09573. RE_TaqI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29748-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPTQAQKVL EAFEDFLKCL DLESYQEKYR PIKTVEQDLP RELNPLPDLY    50
    DHYWKPNGNT LHFPDFETFF DQWWEKRLRP LNEFIRKYFW GCSYEFVRLG 100
    LEARLYRTAV SIWTQFHFCY RWNASCQLRL TATWELDAQG IDAQIQAEDR 150
    LIGIQIKKET YRSEAREGNR FLRRREHSAL LEVPYTLQSP EELERKAQRA 200
    RTREEAYRLW VKIAHHLERL PNGFVIFRES YVKDLENFLK QNATTLSGLI 250
    PWDKVAREAL TGP 263
    Length:263
    Mass (Da):31,584
    Last modified:March 29, 2005 - v2
    Checksum:i10EF1995DB291D98
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti119 – 1191C → S in AAA27490. (PubMed:1339363)Curated
    Sequence conflicti119 – 1191C → S in AAA27489. (PubMed:1339363)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76680 Genomic DNA. Translation: AAA27490.1.
    M74795 Genomic DNA. Translation: AAA27489.1.
    AP008226 Genomic DNA. Translation: BAD71407.1.
    RefSeqiWP_011228787.1. NC_006461.1.
    YP_144850.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD71407; BAD71407; BAD71407.
    GeneIDi3170138.
    KEGGittj:TTHA1584.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76680 Genomic DNA. Translation: AAA27490.1 .
    M74795 Genomic DNA. Translation: AAA27489.1 .
    AP008226 Genomic DNA. Translation: BAD71407.1 .
    RefSeqi WP_011228787.1. NC_006461.1.
    YP_144850.1. NC_006461.1.

    3D structure databases

    ProteinModelPortali P29748.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 300852.TTHA1584.

    Protein family/group databases

    REBASEi 1831. TthHB8I.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD71407 ; BAD71407 ; BAD71407 .
    GeneIDi 3170138.
    KEGGi ttj:TTHA1584.

    Phylogenomic databases

    eggNOGi NOG273292.
    OMAi ARVNEIN.
    OrthoDBi EOG61KBSJ.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-1623-MONOMER.

    Family and domain databases

    InterProi IPR019073. Restrct_endonuc_II_TaqI.
    [Graphical view ]
    Pfami PF09573. RE_TaqI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of genes encoding the TthHB8I restriction and modification enzymes: comparison with the isoschizomeric TaqI enzymes."
      Barany F., Danzitz M., Zebala J., Mayer A.
      Gene 112:3-12(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.

    Entry informationi

    Entry nameiT2T8_THET8
    AccessioniPrimary (citable) accession number: P29748
    Secondary accession number(s): Q5SHZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3