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P29748 (T2T8_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme TthHB8I

Short name=R.TthHB8I
EC=3.1.21.4
Alternative name(s):
Endonuclease TthHB8I
Type II restriction enzyme TthHB8I
Gene names
Name:tthHB8IR
Ordered Locus Names:TTHA1584
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence TCGA and cleaves after T-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263Type-2 restriction enzyme TthHB8I
PRO_0000077370

Experimental info

Sequence conflict1191C → S in AAA27490. Ref.1
Sequence conflict1191C → S in AAA27489. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P29748 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: 10EF1995DB291D98

FASTA26331,584
        10         20         30         40         50         60 
MAPTQAQKVL EAFEDFLKCL DLESYQEKYR PIKTVEQDLP RELNPLPDLY DHYWKPNGNT 

        70         80         90        100        110        120 
LHFPDFETFF DQWWEKRLRP LNEFIRKYFW GCSYEFVRLG LEARLYRTAV SIWTQFHFCY 

       130        140        150        160        170        180 
RWNASCQLRL TATWELDAQG IDAQIQAEDR LIGIQIKKET YRSEAREGNR FLRRREHSAL 

       190        200        210        220        230        240 
LEVPYTLQSP EELERKAQRA RTREEAYRLW VKIAHHLERL PNGFVIFRES YVKDLENFLK 

       250        260 
QNATTLSGLI PWDKVAREAL TGP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of genes encoding the TthHB8I restriction and modification enzymes: comparison with the isoschizomeric TaqI enzymes."
Barany F., Danzitz M., Zebala J., Mayer A.
Gene 112:3-12(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M76680 Genomic DNA. Translation: AAA27490.1.
M74795 Genomic DNA. Translation: AAA27489.1.
AP008226 Genomic DNA. Translation: BAD71407.1.
RefSeqYP_144850.1. NC_006461.1.

3D structure databases

ProteinModelPortalP29748.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1584.

Protein family/group databases

REBASE1831. TthHB8I.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71407; BAD71407; BAD71407.
GeneID3170138.
KEGGttj:TTHA1584.

Phylogenomic databases

eggNOGNOG273292.
OMAARVNEIN.
OrthoDBEOG61KBSJ.
ProtClustDBCLSK2395607.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1623-MONOMER.

Family and domain databases

InterProIPR019073. Restrct_endonuc_II_TaqI.
[Graphical view]
PfamPF09573. RE_TaqI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2T8_THET8
AccessionPrimary (citable) accession number: P29748
Secondary accession number(s): Q5SHZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 29, 2005
Last modified: April 16, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries