Reviewed, UniProtKB/Swiss-Prot P29747 (CREBA_DROME)

Names and origin

Protein names

Recommended name:
    Cyclic AMP response element-binding protein A
      Short name=cAMP response element-binding protein A
      Short name=dCREB-A
Alternative name(s):
    Box B-binding factor 2
      Short name=BBF-2

Gene names

Name:	CrebA
Synonyms:	Bbbf2
ORF Names:	CG7450

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional activator. Binds to fat body-specific enhancers of alcohol dehydrogenase (ADH) and yolk protein genes. BBF-2 may play a role in fat body gene expression. It binds the consensus sequence 5'-T[AC]NACGTAN[TG]C-3'. Ref.1 Ref.2
Subunit structure	May bind DNA as heterodimers with other bZIP proteins.
Subcellular location	Nucleus.
Tissue specificity	In all cell types examined, including developing salivary gland in embryos and in adults, brain and optic lobe cell bodies, salivary gland, midgut epithelial cells of the cardia, female ovarian columnar follicle cells and male seminal vesicle, ejaculatory duct, and ejaculatory bulb. Ref.2
Developmental stage	Present throughout development.
Sequence similarities	Belongs to the bZIP family. Contains 1 bZIP domain.
Sequence caution	The sequence AAU21396.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chitin-based larval cuticle pattern formation Inferred from mutant phenotype. Source: FlyBase dorsal/ventral pattern formation Inferred from genetic interaction. Source: FlyBase regulation of transcription from RNA polymerase II promoter Ref.2 Inferred from direct assay. Source: FlyBase salivary gland morphogenesis Non-traceable author statement. Source: FlyBase transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: FlyBase
Molecular function	RNA polymerase II transcription factor activity Ref.1 Ref.2 Inferred from direct assay. Source: UniProtKB protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Cyclic AMP response element-binding protein A		PRO_0000076635
Regions
	Domain	469 – 504	36	Leucine-zipper
	DNA binding	443 – 463	21	Basic motif
Amino acid modifications
	Modified residue	75	1	Phosphoserine Ref.8
	Modified residue	79	1	Phosphoserine Ref.8
	Modified residue	82	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	114	1	Y → C Ref.1
	Sequence conflict	114	1	Y → C Ref.2
	Sequence conflict	277 – 278	2	QQ → HE in CAA45771. Ref.1
	Sequence conflict	305	1	D → G Ref.1
	Sequence conflict	305	1	D → G Ref.7
	Sequence conflict	316	1	L → T in CAA45771. Ref.1
	Sequence conflict	338 – 364	27	APLSP…VRVSR → RHCRPTQPFPSLWPIQPAVS HPYGSA in CAA45771. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29747-1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 25EA48DAE2ACE7E6	FASTA	516	56,342
	10 20 30 40 50 60 MEFYDGDLKD IWDSDLDPES LKISPDHDMH DWLFDRDVKD PTVILNDKLI SDALLNGTQP 70 80 90 100 110 120 IKTEHSYSLS SDVDSLPDSP KSLQAKIEDM DDECFPAISP KTATNGRVTI DPKYLTFHVP 130 140 150 160 170 180 PTHATPISRL SSNPALNTSV ADLTRSSGLQ SLQAHQPHHG SGSSHVVVAN LEHFQLPQHL 190 200 210 220 230 240 YDNDCSSSVS SLRDGSMSPD ICSDIEIDES AIKDEPMSPD SSCPASPTSQ ASSSQHQLSL 250 260 270 280 290 300 NLAHLQSEML FEPKHCGLLL TASSNSNNSL IKSQQRQQQI LGQDNLLMAK MEIKSEKQST 310 320 330 340 350 360 SNSSDKSHAH GYGIPLTPPS SLPSDDSEGN LSPEHLFAPL SPNATVSISV ANPAGGESSV 370 380 390 400 410 420 RVSRTAASIT RSSSGSASAS GSSTSSTVTT TRQPIHTPLI SSQPKGSTGT LLLTEEEKRT 430 440 450 460 470 480 LLAEGYPIPQ KLPLTKAEEK SLKKIRRKIK NKISAQESRR KKKEYMDQLE RRVEILVTEN 490 500 510 HDYKKRLEGL EETNANLLSQ LHKLQALVSK HNVKKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements." Abel T., Bhatt R., Maniatis T. Genes Dev. 6:466-480(1992) [PubMed: 1532159] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: Oregon-R. Tissue: Embryo.
[2]	"A cyclic AMP-responsive element-binding transcriptional activator in Drosophila melanogaster, dCREB-A, is a member of the leucine zipper family." Smolik S.M., Rose T.M., Goodman R.H. Mol. Cell. Biol. 12:4123-4131(1992) [PubMed: 1508208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Embryo.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-18. Strain: Berkeley. Tissue: Embryo.
[6]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[7]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 221-516. Strain: Berkeley. Tissue: Embryo.
[8]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75; SER-79 AND SER-82, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64429 mRNA. Translation: CAA45771.1. M87038 mRNA. Translation: AAA28427.1. AE014296 Genomic DNA. Translation: AAF49621.2. AY122251 mRNA. Translation: AAU21396.1. Sequence problems. BT011074 mRNA. Translation: AAR82739.1.
PIR	A42140. A44494.
RefSeq	NP_524087.3. NP_996096.1.
UniGene	Dm.6979
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P29747. 4 interactions.
Proteomic databases
PRIDE	P29747.
Genome annotation databases
Ensembl	FBgn0004396. Drosophila melanogaster. [Contig view]
GeneID	39682.
KEGG	dme:Dmel_CG7450.
Organism-specific databases
FlyBase	FBgn0004396. CrebA.
Phylogenomic databases
HOGENOM	P29747.
OMA	P29747. EYMDQLE.
Gene expression databases
ArrayExpress	P29747.
GermOnline	CG7450. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011616. bZIP_1. IPR004827. TF_bZIP. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	814852.

Entry information

Entry name

CREBA_DROME

Accession

Primary (citable) accession number: P29747
Secondary accession number(s): Q24282 , Q64M72, Q8MQX0, Q9VUQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	May 10, 2004
Last modified:	June 16, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project