P29747 (CREBA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclic AMP response element-binding protein A
Short name=cAMP response element-binding protein A
Short name=dCREB-A

Alternative name(s):
Box B-binding factor 2
Short name=BBF-2

Gene names

Name:	CrebA
Synonyms:	Bbbf2
ORF Names:	CG7450

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator. Binds to fat body-specific enhancers of alcohol dehydrogenase (ADH) and yolk protein genes. BBF-2 may play a role in fat body gene expression. It binds the consensus sequence 5'-T[AC]NACGTAN[TG]C-3'. Ref.1 Ref.2
Subunit structure	May bind DNA as heterodimers with other bZIP proteins.
Subcellular location	Nucleus.
Tissue specificity	In all cell types examined, including developing salivary gland in embryos and in adults, brain and optic lobe cell bodies, salivary gland, midgut epithelial cells of the cardia, female ovarian columnar follicle cells and male seminal vesicle, ejaculatory duct, and ejaculatory bulb. Ref.2
Developmental stage	Present throughout development.
Sequence similarities	Belongs to the bZIP family. Contains 1 bZIP (basic-leucine zipper) domain.
Sequence caution	The sequence AAU21396.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chitin-based larval cuticle pattern formation Inferred from mutant phenotype PubMed 15901661PubMed 9006079. Source: FlyBase dorsal/ventral pattern formation Inferred from genetic interaction PubMed 9006079. Source: FlyBase positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.2. Source: FlyBase salivary gland morphogenesis Non-traceable author statement PubMed 11598957. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.1PubMed 19253394. Source: FlyBase
Molecular_function	enhancer sequence-specific DNA binding Inferred from direct assay PubMed 21041443. Source: FlyBase protein homodimerization activity Inferred from sequence or structural similarity PubMed 12176927. Source: FlyBase sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.2. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Cyclic AMP response element-binding protein A		PRO_0000076635
Regions
	Domain	441 – 504	64	bZIP
	Region	443 – 463	21	Basic motif By similarity
	Region	469 – 476	8	Leucine-zipper By similarity
Amino acid modifications
	Modified residue	75	1	Phosphoserine Ref.8
	Modified residue	79	1	Phosphoserine Ref.8
	Modified residue	82	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	114	1	Y → C in CAA45771. Ref.1
	Sequence conflict	114	1	Y → C in AAA28427. Ref.2
	Sequence conflict	277 – 278	2	QQ → HE in CAA45771. Ref.1
	Sequence conflict	305	1	D → G in CAA45771. Ref.1
	Sequence conflict	305	1	D → G in AAR82739. Ref.7
	Sequence conflict	316	1	L → T in CAA45771. Ref.1
	Sequence conflict	338 – 364	27	APLSP…VRVSR → RHCRPTQPFPSLWPIQPAVS HPYGSA in CAA45771. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29747 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 25EA48DAE2ACE7E6	FASTA	516	56,342
	10 20 30 40 50 60 MEFYDGDLKD IWDSDLDPES LKISPDHDMH DWLFDRDVKD PTVILNDKLI SDALLNGTQP 70 80 90 100 110 120 IKTEHSYSLS SDVDSLPDSP KSLQAKIEDM DDECFPAISP KTATNGRVTI DPKYLTFHVP 130 140 150 160 170 180 PTHATPISRL SSNPALNTSV ADLTRSSGLQ SLQAHQPHHG SGSSHVVVAN LEHFQLPQHL 190 200 210 220 230 240 YDNDCSSSVS SLRDGSMSPD ICSDIEIDES AIKDEPMSPD SSCPASPTSQ ASSSQHQLSL 250 260 270 280 290 300 NLAHLQSEML FEPKHCGLLL TASSNSNNSL IKSQQRQQQI LGQDNLLMAK MEIKSEKQST 310 320 330 340 350 360 SNSSDKSHAH GYGIPLTPPS SLPSDDSEGN LSPEHLFAPL SPNATVSISV ANPAGGESSV 370 380 390 400 410 420 RVSRTAASIT RSSSGSASAS GSSTSSTVTT TRQPIHTPLI SSQPKGSTGT LLLTEEEKRT 430 440 450 460 470 480 LLAEGYPIPQ KLPLTKAEEK SLKKIRRKIK NKISAQESRR KKKEYMDQLE RRVEILVTEN 490 500 510 HDYKKRLEGL EETNANLLSQ LHKLQALVSK HNVKKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Drosophila CREB/ATF transcriptional activator binds to both fat body- and liver-specific regulatory elements." Abel T., Bhatt R., Maniatis T. Genes Dev. 6:466-480(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: Oregon-R. Tissue: Embryo.
[2]	"A cyclic AMP-responsive element-binding transcriptional activator in Drosophila melanogaster, dCREB-A, is a member of the leucine zipper family." Smolik S.M., Rose T.M., Goodman R.H. Mol. Cell. Biol. 12:4123-4131(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Embryo.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-18. Strain: Berkeley. Tissue: Embryo.
[6]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[7]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 221-516. Strain: Berkeley. Tissue: Embryo.
[8]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75; SER-79 AND SER-82, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64429 mRNA. Translation: CAA45771.1. M87038 mRNA. Translation: AAA28427.1. AE014296 Genomic DNA. Translation: AAF49621.2. AY122251 mRNA. Translation: AAU21396.1. Sequence problems. BT011074 mRNA. Translation: AAR82739.1.
PIR	A42140. A44494.
RefSeq	NP_524087.3. NM_079363.4. NP_996096.1. NM_206374.1.
UniGene	Dm.6979.
3D structure databases
ProteinModelPortal	P29747.
SMR	P29747. Positions 448-492.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-883694.
Proteomic databases
PaxDb	P29747.
PRIDE	P29747.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0075557; FBpp0075311; FBgn0004396. FBtr0075558; FBpp0089339; FBgn0004396.
GeneID	39682.
KEGG	dme:Dmel_CG7450.
Organism-specific databases
CTD	39682.
FlyBase	FBgn0004396. CrebA.
Phylogenomic databases
eggNOG	NOG294764.
InParanoid	P29747.
OMA	PDICSDI.
OrthoDB	EOG4Q575N.
PhylomeDB	P29747.
Gene expression databases
Bgee	P29747.
GermOnline	CG7450. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004827. bZIP. IPR008917. Euk_TF_DNA-bd. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
SUPFAM	SSF47454. Euk_transcr_DNA. 1 hit.
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CrebA. drosophila.
GenomeRNAi	39682.
NextBio	814852.

Entry information

Entry name

CREBA_DROME

Accession

Primary (citable) accession number: P29747
Secondary accession number(s): Q24282 , Q64M72, Q8MQX0, Q9VUQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	May 10, 2004
Last modified:	May 1, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project