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P29745 (PEPT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptidase T
EC=3.4.11.4
Alternative name(s):
Aminotripeptidase
Short name=Tripeptidase
Tripeptide aminopeptidase
Gene names
Name:pepT
Ordered Locus Names:b1127, JW1113
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves the N-terminal amino acid of tripeptides By similarity. HAMAP MF_00550

Catalytic activity

Release of the N-terminal residue from a tripeptide. HAMAP MF_00550

Cofactor

Binds 2 zinc ions per subunit. Ref.6

Subunit structure

Homodimer Probable. Ref.6

Subcellular location

Cytoplasm By similarity HAMAP MF_00550.

Sequence similarities

Belongs to the peptidase M20B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 408408Peptidase T HAMAP MF_00550
PRO_0000185292

Sites

Active site801 By similarity
Active site1731Proton acceptor By similarity
Metal binding781Zinc 1
Metal binding1401Zinc 1
Metal binding1401Zinc 2
Metal binding1741Zinc 2
Metal binding1961Zinc 1
Metal binding3791Zinc 2

Secondary structure

1 ....................................................................... 408
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29745 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 35751A1DF4CE6DA2

FASTA40844,923
        10         20         30         40         50         60 
MDKLLERFLN YVSLDTQSKA GVRQVPSTEG QWKLLHLLKE QLEEMGLINV TLSEKGTLMA 

        70         80         90        100        110        120 
TLPANVPGDI PAIGFISHVD TSPDCSGKNV NPQIVENYRG GDIALGIGDE VLSPVMFPVL 

       130        140        150        160        170        180 
HQLLGQTLIT TDGKTLLGAD DKAGIAEIMT ALAVLQQKKI PHGDIRVAFT PDEEVGKGAK 

       190        200        210        220        230        240 
HFDVDAFDAR WAYTVDGGGV GELEFENFNA ASVNIKIVGN NVHPGTAKGV MVNALSLAAR 

       250        260        270        280        290        300 
IHAEVPADES PEMTEGYEGF YHLASMKGTV ERADMHYIIR DFDRKQFEAR KRKMMEIAKK 

       310        320        330        340        350        360 
VGKGLHPDCY IELVIEDSYY NMREKVVEHP HILDIAQQAM RDCDIEPELK PIRGGTDGAQ 

       370        380        390        400 
LSFMGLPCPN LFTGGYNYHG KHEFVTLEGM EKAVQVIVRI AELTAQRK

« Hide

References

« Hide 'large scale' references
[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome."
Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K.
J. Biol. Chem. 266:20928-20933(1991) [PubMed: 1939142] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
Strain: K12.
[5]"Physical mapping of the Escherichia coli pepT and potABCD genes."
Lombardo M.-J., Miller C.G., Rudd K.E.
J. Bacteriol. 175:7745-7746(1993) [PubMed: 8244951] [Abstract]
Cited for: IDENTIFICATION.
[6]"Structural analysis of a set of proteins resulting from a bacterial genomics project."
Badger J., Sauder J.M., Adams J.M., Antonysamy S., Bain K., Bergseid M.G., Buchanan S.G., Buchanan M.D., Batiyenko Y., Christopher J.A., Emtage S., Eroshkina A., Feil I., Furlong E.B., Gajiwala K.S., Gao X., He D., Hendle J. expand/collapse author list , Huber A., Hoda K., Kearins P., Kissinger C., Laubert B., Lewis H.A., Lin J., Loomis K., Lorimer D., Louie G., Maletic M., Marsh C.D., Miller I., Molinari J., Muller-Dieckmann H.J., Newman J.M., Noland B.W., Pagarigan B., Park F., Peat T.S., Post K.W., Radojicic S., Ramos A., Romero R., Rutter M.E., Sanderson W.E., Schwinn K.D., Tresser J., Winhoven J., Wright T.A., Wu L., Xu J., Harris T.J.R.
Proteins 60:787-796(2005) [PubMed: 16021622] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 2-408 IN COMPLEX WITH ZINC, COFACTOR, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U00096 Genomic DNA. Translation: AAC74211.1.
AP009048 Genomic DNA. Translation: BAA35949.1.
M64519 Genomic DNA. No translation available.
PIRD64857.
RefSeqNP_415645.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VIXX-ray2.50A/B2-408[»]
ProteinModelPortalP29745.
SMRP29745. Positions 1-408.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10461N.
IntActP29745. 17 interactions.
MINTMINT-1219063.

Protein family/group databases

MEROPSM20.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000003789; EBESCP00000003789; EBESCG00000003098.
EBESCT00000015275; EBESCP00000014566; EBESCG00000014335.
GeneID946333.
GenomeReviewsGene locus JW1113 in contig AP009048_GR.
Gene locus b1127 in contig U00096_GR.
KEGGecj:JW1113.
eco:b1127.
PATRIC32117503. VBIEscCol129921_1174.

Organism-specific databases

EchoBASEEB1511.
EcoGeneEG11549. pepT.

Phylogenomic databases

eggNOGCOG2195.
GeneTreeEBGT00050000009694.
HOGENOMHBG288638.
OMAHMDTAPD.
PhylomeDBP29745.
ProtClustDBPRK05469.

Enzyme and pathway databases

BioCycEcoCyc:EG11549-MONOMER.
MetaCyc:EG11549-MONOMER.

Gene expression databases

GenevestigatorP29745.

Family and domain databases

HAMAPMF_00550. Aminopeptidase_M20.
[Tree]
InterProIPR001261. ArgE/DapE_CS.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
IPR010161. Peptidase_M20B.
[Graphical view]
KOK01258.
PfamPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFPIRSF037215. Peptidase_M20B. 1 hit.
SUPFAMSSF55031. Peptidase_M20_dimer. 1 hit.
TIGRFAMsTIGR01882. Peptidase-T. 1 hit.
PROSITEPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePEPT_ECOLI
AccessionPrimary (citable) accession number: P29745
Secondary accession number(s): P77794
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents