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Protein

Peptidase T

Gene

pepT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves the N-terminal amino acid of tripeptides.By similarity

Catalytic activityi

Release of the N-terminal residue from a tripeptide.

Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi78Zinc 11 Publication1
Active sitei80By similarity1
Metal bindingi140Zinc 11 Publication1
Metal bindingi140Zinc 21 Publication1
Active sitei173Proton acceptorBy similarity1
Metal bindingi174Zinc 21 Publication1
Metal bindingi196Zinc 11 Publication1
Metal bindingi379Zinc 21 Publication1

GO - Molecular functioni

  • metallopeptidase activity Source: UniProtKB-KW
  • tripeptide aminopeptidase activity Source: EcoCyc
  • zinc ion binding Source: InterPro

GO - Biological processi

  • peptide metabolic process Source: EcoCyc

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG11549-MONOMER
MetaCyc:EG11549-MONOMER

Protein family/group databases

MEROPSiM20.003

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidase T (EC:3.4.11.4)
Alternative name(s):
Aminotripeptidase
Short name:
Tripeptidase
Tripeptide aminopeptidase
Gene namesi
Name:pepT
Ordered Locus Names:b1127, JW1113
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11549 pepT

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001852921 – 408Peptidase TAdd BLAST408

Proteomic databases

PaxDbiP29745
PRIDEiP29745

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4261675, 73 interactors
DIPiDIP-10461N
IntActiP29745, 20 interactors
STRINGi316385.ECDH10B_1199

Structurei

Secondary structure

1408
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 13Combined sources12
Beta strandi23 – 28Combined sources6
Helixi30 – 43Combined sources14
Turni44 – 46Combined sources3
Beta strandi48 – 52Combined sources5
Beta strandi58 – 62Combined sources5
Beta strandi73 – 78Combined sources6
Beta strandi93 – 95Combined sources3
Beta strandi103 – 105Combined sources3
Turni106 – 109Combined sources4
Beta strandi110 – 112Combined sources3
Turni114 – 116Combined sources3
Helixi118 – 122Combined sources5
Beta strandi128 – 130Combined sources3
Beta strandi133 – 135Combined sources3
Helixi139 – 157Combined sources19
Beta strandi165 – 171Combined sources7
Helixi173 – 175Combined sources3
Turni178 – 181Combined sources4
Helixi184 – 187Combined sources4
Beta strandi190 – 195Combined sources6
Beta strandi202 – 204Combined sources3
Beta strandi209 – 218Combined sources10
Helixi224 – 226Combined sources3
Turni228 – 230Combined sources3
Helixi234 – 244Combined sources11
Turni251 – 253Combined sources3
Beta strandi260 – 283Combined sources24
Helixi284 – 302Combined sources19
Beta strandi310 – 319Combined sources10
Helixi323 – 327Combined sources5
Helixi331 – 342Combined sources12
Beta strandi352 – 354Combined sources3
Helixi357 – 361Combined sources5
Turni362 – 365Combined sources4
Beta strandi374 – 377Combined sources4
Beta strandi384 – 386Combined sources3
Helixi387 – 406Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VIXX-ray2.50A/B2-408[»]
ProteinModelPortaliP29745
SMRiP29745
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29745

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20B family.Curated

Phylogenomic databases

eggNOGiENOG4105D42 Bacteria
COG2195 LUCA
HOGENOMiHOG000032390
InParanoidiP29745
KOiK01258
OMAiGHNFHGK
PhylomeDBiP29745

Family and domain databases

CDDicd03892 M20_peptT, 1 hit
HAMAPiMF_00550 Aminopeptidase_M20, 1 hit
InterProiView protein in InterPro
IPR001261 ArgE/DapE_CS
IPR036264 Bact_exopeptidase_dim_dom
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
IPR010161 Peptidase_M20B
PANTHERiPTHR42994:SF1 PTHR42994:SF1, 1 hit
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
PIRSFiPIRSF037215 Peptidase_M20B, 1 hit
SUPFAMiSSF55031 SSF55031, 1 hit
TIGRFAMsiTIGR01882 peptidase-T, 1 hit
PROSITEiView protein in PROSITE
PS00758 ARGE_DAPE_CPG2_1, 1 hit
PS00759 ARGE_DAPE_CPG2_2, 1 hit

Sequencei

Sequence statusi: Complete.

P29745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKLLERFLN YVSLDTQSKA GVRQVPSTEG QWKLLHLLKE QLEEMGLINV
60 70 80 90 100
TLSEKGTLMA TLPANVPGDI PAIGFISHVD TSPDCSGKNV NPQIVENYRG
110 120 130 140 150
GDIALGIGDE VLSPVMFPVL HQLLGQTLIT TDGKTLLGAD DKAGIAEIMT
160 170 180 190 200
ALAVLQQKKI PHGDIRVAFT PDEEVGKGAK HFDVDAFDAR WAYTVDGGGV
210 220 230 240 250
GELEFENFNA ASVNIKIVGN NVHPGTAKGV MVNALSLAAR IHAEVPADES
260 270 280 290 300
PEMTEGYEGF YHLASMKGTV ERADMHYIIR DFDRKQFEAR KRKMMEIAKK
310 320 330 340 350
VGKGLHPDCY IELVIEDSYY NMREKVVEHP HILDIAQQAM RDCDIEPELK
360 370 380 390 400
PIRGGTDGAQ LSFMGLPCPN LFTGGYNYHG KHEFVTLEGM EKAVQVIVRI

AELTAQRK
Length:408
Mass (Da):44,923
Last modified:November 1, 1997 - v2
Checksum:i35751A1DF4CE6DA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74211.1
AP009048 Genomic DNA Translation: BAA35949.1
M64519 Genomic DNA No translation available.
PIRiD64857
RefSeqiNP_415645.1, NC_000913.3
WP_000359434.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74211; AAC74211; b1127
BAA35949; BAA35949; BAA35949
GeneIDi946333
KEGGiecj:JW1113
eco:b1127
PATRICifig|1411691.4.peg.1139

Similar proteinsi

Entry informationi

Entry nameiPEPT_ECOLI
AccessioniPrimary (citable) accession number: P29745
Secondary accession number(s): P77794
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health