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Protein

Clathrin heavy chain

Gene

Chc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

GO - Molecular functioni

GO - Biological processi

  • chemical synaptic transmission Source: FlyBase
  • compound eye development Source: FlyBase
  • compound eye retinal cell programmed cell death Source: FlyBase
  • dsRNA transport Source: FlyBase
  • endocytosis Source: FlyBase
  • extracellular matrix organization Source: FlyBase
  • intracellular protein transport Source: InterPro
  • liquid clearance, open tracheal system Source: FlyBase
  • neurotransmitter secretion Source: FlyBase
  • oocyte microtubule cytoskeleton polarization Source: FlyBase
  • pole plasm oskar mRNA localization Source: FlyBase
  • positive regulation of endocytosis Source: FlyBase
  • positive regulation of Notch signaling pathway Source: FlyBase
  • puparial adhesion Source: FlyBase
  • regulation of growth Source: FlyBase
  • regulation of tube length, open tracheal system Source: FlyBase
  • secretory granule organization Source: FlyBase
  • sperm individualization Source: FlyBase
  • synaptic vesicle coating Source: FlyBase
  • synaptic vesicle exocytosis Source: FlyBase
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-DME-171052. LDL-mediated lipid transport.
R-DME-177504. Retrograde neurotrophin signalling.
R-DME-190873. Gap junction degradation.
R-DME-196025. Formation of annular gap junctions.
R-DME-432720. Lysosome Vesicle Biogenesis.
R-DME-432722. Golgi Associated Vesicle Biogenesis.
R-DME-437239. Recycling pathway of L1.
R-DME-5099900. WNT5A-dependent internalization of FZD4.
R-DME-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-DME-8856828. Clathrin-mediated endocytosis.
R-DME-8866427. VLDLR internalisation and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin heavy chain
Gene namesi
Name:Chc
ORF Names:CG9012
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000319. Chc.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: FlyBase
  • clathrin-coated pit Source: FlyBase
  • clathrin-coated vesicle Source: FlyBase
  • clathrin coat of coated pit Source: InterPro
  • clathrin coat of trans-Golgi network vesicle Source: InterPro
  • clathrin complex Source: FlyBase
  • clathrin vesicle coat Source: FlyBase
  • coated vesicle Source: FlyBase
  • cytoplasmic vesicle Source: FlyBase
  • lipid particle Source: FlyBase
  • perinuclear region of cytoplasm Source: FlyBase
  • plasma membrane Source: FlyBase
  • secretory granule Source: FlyBase
  • synaptic vesicle Source: FlyBase
  • trans-Golgi network Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002057831 – 1678Clathrin heavy chainAdd BLAST1678

Proteomic databases

PaxDbiP29742.
PRIDEiP29742.

Expressioni

Gene expression databases

BgeeiFBgn0000319.
ExpressionAtlasiP29742. baseline.
GenevisibleiP29742. DM.

Interactioni

Subunit structurei

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat (By similarity). Interacts with sau (PubMed:24786584).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BicDP165685EBI-160368,EBI-112159

GO - Molecular functioni

Protein-protein interaction databases

BioGridi58885. 91 interactors.
DIPiDIP-17970N.
IntActiP29742. 10 interactors.
MINTiMINT-1330735.
STRINGi7227.FBpp0073966.

Structurei

3D structure databases

ProteinModelPortaliP29742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati538 – 684CHCR 1Add BLAST147
Repeati687 – 829CHCR 2Add BLAST143
Repeati834 – 973CHCR 3Add BLAST140
Repeati980 – 1125CHCR 4Add BLAST146
Repeati1129 – 1270CHCR 5Add BLAST142
Repeati1275 – 1421CHCR 6Add BLAST147
Repeati1424 – 1567CHCR 7Add BLAST144

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 67WD40-like repeat 1Add BLAST44
Regioni68 – 107WD40-like repeat 2Add BLAST40
Regioni108 – 149WD40-like repeat 3Add BLAST42
Regioni150 – 195WD40-like repeat 4Add BLAST46
Regioni196 – 257WD40-like repeat 5Add BLAST62
Regioni258 – 301WD40-like repeat 6Add BLAST44
Regioni302 – 330WD40-like repeat 7Add BLAST29
Regioni1334 – 1643Involved in binding clathrin light chainBy similarityAdd BLAST310
Regioni1552 – 1677TrimerizationBy similarityAdd BLAST126

Domaini

The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.
The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It constitutes a major protein-protein interaction node (By similarity).By similarity

Sequence similaritiesi

Belongs to the clathrin heavy chain family.Curated
Contains 7 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0985. Eukaryota.
ENOG410XPH1. LUCA.
InParanoidiP29742.
KOiK04646.
OMAiDPEIWAF.
OrthoDBiEOG091G009O.
PhylomeDBiP29742.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
2.130.10.110. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 5 hits.
[Graphical view]
PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTiSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF50989. SSF50989. 1 hit.
PROSITEiPS50236. CHCR. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQPLPIRFQ EHLQLTNVGI NANSFSFSTL TMESDKFICV REKVNDTAQV
60 70 80 90 100
VIIDMNDATN PTRRPISADS AIMNPASKVI ALKAQKTLQI FNIEMKSKMK
110 120 130 140 150
AHTMNEDVVF WKWISLNTLA LVTETSVFHW SMEGDSMPQK MFDRHSSLNG
160 170 180 190 200
CQIINYRCNA SQQWLLLVGI SALPSRVAGA MQLYSVERKV SQAIEGHAAS
210 220 230 240 250
FATFKIDANK EPTTLFCFAV RTATGGKLHI IEVGAPPNGN QPFAKKAVDV
260 270 280 290 300
FFPPEAQNDF PVAMQVSAKY DTIYLITKYG YIHLYDMETA TCIYMNRISA
310 320 330 340 350
DTIFVTAPHE ASGGIIGVNR KGQVLSVTVD EEQIIPYINT VLQNPDLALR
360 370 380 390 400
MAVRNNLAGA EDLFVRKFNK LFTAGQYAEA AKVAALAPKA ILRTPQTIQR
410 420 430 440 450
FQQVQTPAGS TTPPLLQYFG ILLDQGKLNK FESLELCRPV LLQGKKQLCE
460 470 480 490 500
KWLKEEKLEC SEELGDLVKA SDLTLALSIY LRANVPNKVI QCFAETGQFQ
510 520 530 540 550
KIVLYAKKVN YTPDYVFLLR SVMRSNPEQG AGFASMLVAE EEPLADINQI
560 570 580 590 600
VDIFMEHSMV QQCTAFLLDA LKHNRPAEGA LQTRLLEMNL MSAPQVADAI
610 620 630 640 650
LGNAMFTHYD RAHIAQLCEK AGLLQRALEH YTDLYDIKRA VVHTHMLNAE
660 670 680 690 700
WLVSFFGTLS VEDSLECLKA MLTANLRQNL QICVQIATKY HEQLTNKALI
710 720 730 740 750
DLFEGFKSYD GLFYFLSSIV NFSQDPEVHF KYIQAACKTN QIKEVERICR
760 770 780 790 800
ESNCYNPERV KNFLKEAKLT DQLPLIIVCD RFDFVHDLVL YLYRNNLQKY
810 820 830 840 850
IEIYVQKVNP SRLPVVVGGL LDVDCSEDII KNLILVVKGQ FSTDELVEEV
860 870 880 890 900
EKRNRLKLLL PWLESRVHEG CVEPATHNAL AKIYIDSNNN PERYLKENQY
910 920 930 940 950
YDSRVVGRYC EKRDPHLACV AYERGLCDRE LIAVCNENSL FKSEARYLVG
960 970 980 990 1000
RRDAELWAEV LSESNPYKRQ LIDQVVQTAL SETQDPDDIS VTVKAFMTAD
1010 1020 1030 1040 1050
LPNELIELLE KIILDSSVFS DHRNLQNLLI LTAIKADRTR VMDYINRLEN
1060 1070 1080 1090 1100
YDAPDIANIA ISNQLYEEAF AIFKKFDVNT SAIQVLIDQV NNLERANEFA
1110 1120 1130 1140 1150
ERCNEPAVWS QLAKAQLQQG LVKEAIDSYI KADDPSAYVD VVDVASKVES
1160 1170 1180 1190 1200
WDDLVRYLQM ARKKARESYI ESELIYAYAR TGRLADLEEF ISGPNHADIQ
1210 1220 1230 1240 1250
KIGNRCFSDG MYDAAKLLYN NVSNFARLAI TLVYLKEFQG AVDSARKANS
1260 1270 1280 1290 1300
TRTWKEVCFA CVDAEEFRLA QMCGLHIVVH ADELEDLINY YQNRGYFDEL
1310 1320 1330 1340 1350
IALLESALGL ERAHMGMFTE LAILYSKFKP SKMREHLELF WSRVNIPKVL
1360 1370 1380 1390 1400
RAAESAHLWS ELVFLYDKYE EYDNAVLAMM AHPTEAWREG HFKDIITKVA
1410 1420 1430 1440 1450
NIELYYKAIE FYLDFKPLLL NDMLLVLAPR MDHTRAVSYF SKTGYLPLVK
1460 1470 1480 1490 1500
PYLRSVQSLN NKAINEALNG LLIDEEDYQG LRNSIDGFDN FDNIALAQKL
1510 1520 1530 1540 1550
EKHELTEFRR IAAYLYKGNN RWKQSVELCK KDKLYKDAME YAAESCKQDI
1560 1570 1580 1590 1600
AEELLGWFLE RDAYDCFAAC LYQCYDLLRP DVILELAWKH KIVDFAMPYL
1610 1620 1630 1640 1650
IQVLREYTTK VDKLELNEAQ REKEDDSTEH KNIIQMEPQL MITAGPAMGI
1660 1670
PPQYAQNYPP GAATVTAAGG RNMGYPYL
Length:1,678
Mass (Da):191,177
Last modified:April 1, 1993 - v1
Checksum:i73CAF8631BEE9BA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14133 mRNA. Translation: CAA78507.1.
AE014298 Genomic DNA. Translation: AAF48522.1.
AE014298 Genomic DNA. Translation: AAN09367.1.
AE014298 Genomic DNA. Translation: AAS65352.1.
AE014298 Genomic DNA. Translation: AAS65353.1.
AE014298 Genomic DNA. Translation: ABW09424.1.
AE014298 Genomic DNA. Translation: ABW09425.1.
AY119615 mRNA. Translation: AAM50269.1.
PIRiS52588.
RefSeqiNP_001096993.1. NM_001103523.2.
NP_001096994.1. NM_001103524.2.
NP_001285300.1. NM_001298371.1.
NP_477042.1. NM_057694.3.
NP_727901.1. NM_167466.2.
NP_996451.1. NM_206728.2.
NP_996452.1. NM_206729.2.
UniGeneiDm.3174.

Genome annotation databases

EnsemblMetazoaiFBtr0074179; FBpp0073966; FBgn0000319.
FBtr0074180; FBpp0073967; FBgn0000319.
FBtr0074181; FBpp0089397; FBgn0000319.
FBtr0074182; FBpp0089398; FBgn0000319.
FBtr0112797; FBpp0111709; FBgn0000319.
FBtr0112798; FBpp0111710; FBgn0000319.
FBtr0339445; FBpp0308531; FBgn0000319.
GeneIDi32537.
KEGGidme:Dmel_CG9012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14133 mRNA. Translation: CAA78507.1.
AE014298 Genomic DNA. Translation: AAF48522.1.
AE014298 Genomic DNA. Translation: AAN09367.1.
AE014298 Genomic DNA. Translation: AAS65352.1.
AE014298 Genomic DNA. Translation: AAS65353.1.
AE014298 Genomic DNA. Translation: ABW09424.1.
AE014298 Genomic DNA. Translation: ABW09425.1.
AY119615 mRNA. Translation: AAM50269.1.
PIRiS52588.
RefSeqiNP_001096993.1. NM_001103523.2.
NP_001096994.1. NM_001103524.2.
NP_001285300.1. NM_001298371.1.
NP_477042.1. NM_057694.3.
NP_727901.1. NM_167466.2.
NP_996451.1. NM_206728.2.
NP_996452.1. NM_206729.2.
UniGeneiDm.3174.

3D structure databases

ProteinModelPortaliP29742.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58885. 91 interactors.
DIPiDIP-17970N.
IntActiP29742. 10 interactors.
MINTiMINT-1330735.
STRINGi7227.FBpp0073966.

Proteomic databases

PaxDbiP29742.
PRIDEiP29742.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0074179; FBpp0073966; FBgn0000319.
FBtr0074180; FBpp0073967; FBgn0000319.
FBtr0074181; FBpp0089397; FBgn0000319.
FBtr0074182; FBpp0089398; FBgn0000319.
FBtr0112797; FBpp0111709; FBgn0000319.
FBtr0112798; FBpp0111710; FBgn0000319.
FBtr0339445; FBpp0308531; FBgn0000319.
GeneIDi32537.
KEGGidme:Dmel_CG9012.

Organism-specific databases

CTDi32537.
FlyBaseiFBgn0000319. Chc.

Phylogenomic databases

eggNOGiKOG0985. Eukaryota.
ENOG410XPH1. LUCA.
InParanoidiP29742.
KOiK04646.
OMAiDPEIWAF.
OrthoDBiEOG091G009O.
PhylomeDBiP29742.

Enzyme and pathway databases

ReactomeiR-DME-171052. LDL-mediated lipid transport.
R-DME-177504. Retrograde neurotrophin signalling.
R-DME-190873. Gap junction degradation.
R-DME-196025. Formation of annular gap junctions.
R-DME-432720. Lysosome Vesicle Biogenesis.
R-DME-432722. Golgi Associated Vesicle Biogenesis.
R-DME-437239. Recycling pathway of L1.
R-DME-5099900. WNT5A-dependent internalization of FZD4.
R-DME-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-DME-8856828. Clathrin-mediated endocytosis.
R-DME-8866427. VLDLR internalisation and degradation.

Miscellaneous databases

ChiTaRSiChc. fly.
GenomeRNAii32537.
PROiP29742.

Gene expression databases

BgeeiFBgn0000319.
ExpressionAtlasiP29742. baseline.
GenevisibleiP29742. DM.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
2.130.10.110. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 5 hits.
[Graphical view]
PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTiSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF50989. SSF50989. 1 hit.
PROSITEiPS50236. CHCR. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLH_DROME
AccessioniPrimary (citable) accession number: P29742
Secondary accession number(s): Q540W2, Q9VXN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.