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P29738 (MYR2_SINAL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myrosinase MB2

EC=3.2.1.147
Alternative name(s):
Sinigrinase
Thioglucosidase
OrganismSinapis alba (White mustard) (Brassica hirta)
Taxonomic identifier3728 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeSinapis

Protein attributes

Sequence length243 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

Catalytic activity

A thioglucoside + H2O = a sugar + a thiol.

Subunit structure

Homodimer.

Subcellular location

Vacuole.

Tissue specificity

In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Cellular componentVacuole
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentvacuole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthioglucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 243›243Myrosinase MB2
PRO_0000063903

Regions

Region180 – 1812Substrate binding By similarity

Sites

Active site1251Nucleophile By similarity
Binding site511Substrate By similarity
Binding site1731Substrate By similarity

Amino acid modifications

Glycosylation301N-linked (GlcNAc...) Potential
Glycosylation2161N-linked (GlcNAc...) Potential

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P29738 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: F79CE00FD7FDB4D7

FASTA24328,184
        10         20         30         40         50         60 
EFFHGWYMEP LTKGRYPDIM RQIVGTRLPN FTEDEAELVA GSYDFLGLNY YVTQYAKPKP 

        70         80         90        100        110        120 
NPYPSEKHTA MDDAGVDLTF KNSRGEYPGP VFAEDANSYY YPKGIYYVMD YFKTKYGNPL 

       130        140        150        160        170        180 
IYITENGIST PGSESRCERI ADYKRINYHC SHLCFLSKVI KEKGVNVRGY FAWALGDNYE 

       190        200        210        220        230        240 
FGKGFTVRFG LSYVNWDDLN DRNLKESGKW YQRFINGTAK NPVKQNFLRS SLSSQNQKKR 


LAC 

« Hide

References

[1]"The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family."
Xue J., Lenman M., Falk A., Rask L.
Plant Mol. Biol. 18:387-398(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Maxi.
Tissue: Seed.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59880 mRNA. Translation: CAA42535.1.
PIRS19148.

3D structure databases

ProteinModelPortalP29738.
SMRP29738. Positions 1-216.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMYR2_SINAL
AccessionPrimary (citable) accession number: P29738
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries