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Protein

Myrosinase MB2

Gene
N/A
Organism
Sinapis alba (White mustard) (Brassica hirta)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51SubstrateBy similarity1
Active sitei125NucleophilePROSITE-ProRule annotation1
Binding sitei173SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase MB2 (EC:3.2.1.147)
Alternative name(s):
Sinigrinase
Thioglucosidase
OrganismiSinapis alba (White mustard) (Brassica hirta)
Taxonomic identifieri3728 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeSinapis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000063903‹1 – 243Myrosinase MB2Add BLAST›243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi30N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi216N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP29738

Expressioni

Tissue specificityi

In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP29738
SMRiP29738
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 181Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR018120 Glyco_hydro_1_AS
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR10353 PTHR10353, 1 hit
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 1 hit
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00572 GLYCOSYL_HYDROL_F1_1, 1 hit

Sequencei

Sequence statusi: Fragment.

P29738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
EFFHGWYMEP LTKGRYPDIM RQIVGTRLPN FTEDEAELVA GSYDFLGLNY
60 70 80 90 100
YVTQYAKPKP NPYPSEKHTA MDDAGVDLTF KNSRGEYPGP VFAEDANSYY
110 120 130 140 150
YPKGIYYVMD YFKTKYGNPL IYITENGIST PGSESRCERI ADYKRINYHC
160 170 180 190 200
SHLCFLSKVI KEKGVNVRGY FAWALGDNYE FGKGFTVRFG LSYVNWDDLN
210 220 230 240
DRNLKESGKW YQRFINGTAK NPVKQNFLRS SLSSQNQKKR LAC
Length:243
Mass (Da):28,184
Last modified:April 1, 1993 - v1
Checksum:iF79CE00FD7FDB4D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59880 mRNA Translation: CAA42535.1
PIRiS19148

Similar proteinsi

Entry informationi

Entry nameiMYR2_SINAL
AccessioniPrimary (citable) accession number: P29738
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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