P29737 (MYR1_SINAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Myrosinase MB1 EC=3.2.1.147 Alternative name(s): Sinigrinase Thioglucosidase
Organism	Sinapis alba (White mustard) (Brassica hirta)
Taxonomic identifier	3728 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Sinapis

Protein attributes

Sequence length	244 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.
Catalytic activity	A thioglucoside + H2O = a sugar + a thiol.
Subunit structure	Homodimer.
Subcellular location	Vacuole.
Tissue specificity	In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Cellular component	Vacuole
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thioglucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 244	›244	Myrosinase MB1		PRO_0000063902
Regions
	Region	180 – 181	2	Substrate binding By similarity
Sites
	Active site	125	1	Nucleophile By similarity
	Binding site	51	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity
Amino acid modifications
	Glycosylation	32	1	N-linked (GlcNAc...) Potential
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P29737 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 1BCC1B6B5F047E2A	FASTA	244	28,163
	10 20 30 40 50 60 EFFHGWYMEP LTKGRYPAIM RKIVGSRLPK FNKTEAKLVT GSYDFLGLNY YVTQYAKPKP 70 80 90 100 110 120 NPYPSETHTA MMDAGVDLTF KNSRGEYPGP VFAEDANSYY YPKGIYYVMD YFKTKYGNPL 130 140 150 160 170 180 IYITENGIST PGSENRCEAI ADYKRIDYLC SHLCFLRKVI KEKGVNVRGY FAWALGDNYE 190 200 210 220 230 240 FGKGFTVRFG LSYVNWDNLD DRNLKESGKW YQRFINGTAK NSAKQDFLRS SLSSQSQKKR LADA

References

[1]	"The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family." Xue J., Lenman M., Falk A., Rask L. Plant Mol. Biol. 18:387-398(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Maxi. Tissue: Seed.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59881 mRNA. Translation: CAA42536.1.
PIR	S19147.
3D structure databases
ProteinModelPortal	P29737.
SMR	P29737. Positions 1-216.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10353. PTHR10353. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MYR1_SINAL

Accession

Primary (citable) accession number: P29737

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program