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P29737

- MYR1_SINAL

UniProt

P29737 - MYR1_SINAL

Protein

Myrosinase MB1

Gene
N/A
Organism
Sinapis alba (White mustard) (Brassica hirta)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.

    Catalytic activityi

    A thioglucoside + H2O = a sugar + a thiol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511SubstrateBy similarity
    Active sitei125 – 1251NucleophilePROSITE-ProRule annotation
    Binding sitei173 – 1731SubstrateBy similarity

    GO - Molecular functioni

    1. thioglucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myrosinase MB1 (EC:3.2.1.147)
    Alternative name(s):
    Sinigrinase
    Thioglucosidase
    OrganismiSinapis alba (White mustard) (Brassica hirta)
    Taxonomic identifieri3728 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeSinapis

    Subcellular locationi

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 244›244Myrosinase MB1PRO_0000063902Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi32 – 321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    In vacuoles called myrosin grains of a certain class of cells, myrosin cells, distributed in the cotyledons and the axis of the embryo as well as in different organs of the growing plant.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP29737.
    SMRiP29737. Positions 1-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni180 – 1812Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P29737-1 [UniParc]FASTAAdd to Basket

    « Hide

    EFFHGWYMEP LTKGRYPAIM RKIVGSRLPK FNKTEAKLVT GSYDFLGLNY    50
    YVTQYAKPKP NPYPSETHTA MMDAGVDLTF KNSRGEYPGP VFAEDANSYY 100
    YPKGIYYVMD YFKTKYGNPL IYITENGIST PGSENRCEAI ADYKRIDYLC 150
    SHLCFLRKVI KEKGVNVRGY FAWALGDNYE FGKGFTVRFG LSYVNWDNLD 200
    DRNLKESGKW YQRFINGTAK NSAKQDFLRS SLSSQSQKKR LADA 244
    Length:244
    Mass (Da):28,163
    Last modified:April 1, 1993 - v1
    Checksum:i1BCC1B6B5F047E2A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59881 mRNA. Translation: CAA42536.1.
    PIRiS19147.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59881 mRNA. Translation: CAA42536.1 .
    PIRi S19147.

    3D structure databases

    ProteinModelPortali P29737.
    SMRi P29737. Positions 1-216.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family."
      Xue J., Lenman M., Falk A., Rask L.
      Plant Mol. Biol. 18:387-398(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: cv. Maxi.
      Tissue: Seed.

    Entry informationi

    Entry nameiMYR1_SINAL
    AccessioniPrimary (citable) accession number: P29737
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3