P29719 (GUNA_PAELA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase A
EC=3.2.1.4

Alternative name(s):
Cellulase A
EG-A
Endo-1,4-beta-glucanase

Gene names

Name:

celA

Organism

Paenibacillus lautus (Bacillus lautus)

Taxonomic identifier

1401 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Paenibacillus

Protein attributes

Sequence length	700 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Post-translational modification	A short form (EG-A-S) arises from post-translational proteolysis of approximately 150 AA at the C-terminus of EG-A-L.
Sequence similarities	Belongs to the glycosyl hydrolase 44 (cellulase J) family. Contains 1 CBM3 (carbohydrate binding type-3) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 700	667	Endoglucanase A		PRO_0000008021
Regions
	Domain	550 – 700	151	CBM3
Sites
	Active site	213	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29719 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 3D5C8CADA53EEE0F	FASTA	700	76,910
	10 20 30 40 50 60 MKTRQRKRLF VSAALAVSLT MTVPMPASVN AAASDVTFTI NTQSERAAIS PNIYGTNQDL 70 80 90 100 110 120 SGTENWSSRR LGGNRLTGYN WENNASSAGR DWLHYSDDFL CGNGGVPDTD CDKPGAVVTA 130 140 150 160 170 180 FHDKSLENGA YSIVTLQMAG YVSRDKNGPV DESETAPSPR WDKVEFAKNA PFSLQPHLND 190 200 210 220 230 240 GQVYMDEEVN FLVNRYGNAS TSTGIKAYSL DNEPALWSET HPRIHPEQLQ AAELVAKSID 250 260 270 280 290 300 LSKAVKNVDP HAEIFGPALY GFGAYLSLQD APGWPSLQGN YSWFIDYYLD QMKNAHTQNG 310 320 330 340 350 360 KRLLDVLDVH WYPEAQGGGQ RIVFGGAGNI DTQKARVQAP RSLWDPAYQE DSWIGTWFSS 370 380 390 400 410 420 YLPLIPKLQS SIQTYYPGTK LAITESSYGG DNHISGGIAT ADALGIFGKY GVYAANYWQT 430 440 450 460 470 480 EDNTDYTSAA YKLYRNYDGN KSGFGSIKVD AATSDTENSS VYASVTDEEN SELHLIVLNK 490 500 510 520 530 540 NFDDPINATF QLSGDKTYTS GRVWGFDQTG SDITEQAAIT NINNNQFTYT LPPLSAYHIV 550 560 570 580 590 600 LKADSTEPVN SDLVVQYKDG DRNNATDNQI KPHFNIQNKG TSPVDLSSLT LRYYFTKDSS 610 620 630 640 650 660 AAMNGWIDWA KLGGSNIQIS FGNHNGADSD TYAELGFSSG AGSIAEGGQS GEIQLRMSKA 670 680 690 700 DWSNFNEAND YSFDGAKTAY IDWDRVTLYQ DGQLVWGIEP

References

[1]	"celA from Bacillus lautus PL236 encodes a novel cellulose-binding endo-beta-1,4-glucanase." Hansen C.K., Diderichsen B., Joergensen P.L. J. Bacteriol. 174:3522-3531(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PL236.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M76588 Genomic DNA. Translation: AAA22303.1.
PIR	B41897.
3D structure databases
ProteinModelPortal	P29719.
SMR	P29719. Positions 33-543.
ModBase	Search...
Protein family/group databases
CAZy	CBM3. Carbohydrate-Binding Module Family 3. GH44. Glycoside Hydrolase Family 44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.40.710. 1 hit.
InterPro	IPR008965. Carb-bd_dom. IPR001956. CBD_3. IPR024745. Glyco_hydro_44. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00942. CBM_3. 1 hit. PF12891. Glyco_hydro_44. 1 hit. [Graphical view]
SMART	SM01067. CBM_3. 1 hit. [Graphical view]
SUPFAM	SSF49384. Cellul_bind. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS51172. CBM3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNA_PAELA

Accession

Primary (citable) accession number: P29719

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program