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Protein

Squalene synthase

Gene

ERG9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol. May also have a regulatory role regulating the flux of isoprene intermediates through the sterol pathway. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. ERG9 is also essential for cell growth in yeast.3 Publications

Miscellaneous

Present with 10800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Mg2+1 Publication

Pathwayi: lanosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (ERG9)
  2. Squalene monooxygenase (ERG1)
  3. Lanosterol synthase (ERG7)
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

GO - Molecular functioni

  • farnesyl-diphosphate farnesyltransferase activity Source: SGD
  • oxidoreductase activity Source: UniProtKB-KW
  • squalene synthase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
  • farnesyl diphosphate metabolic process Source: GO_Central
  • isoprenoid biosynthetic process Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase, Transferase
Biological processIsoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandMagnesium, NADP

Enzyme and pathway databases

BioCyciMetaCyc:YHR190W-MONOMER.
YEAST:YHR190W-MONOMER.
ReactomeiR-SCE-191273. Cholesterol biosynthesis.
UniPathwayiUPA00767; UER00751.

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:ERG9
Ordered Locus Names:YHR190W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR190W.
SGDiS000001233. ERG9.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei291 – 311HelicalSequence analysisAdd BLAST21
Transmembranei421 – 441HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3271930.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000674541 – 444Squalene synthaseAdd BLAST444

Proteomic databases

MaxQBiP29704.
PRIDEiP29704.

Interactioni

Protein-protein interaction databases

BioGridi36624. 338 interactors.
DIPiDIP-4576N.
IntActiP29704. 7 interactors.
MINTiMINT-491875.
STRINGi4932.YHR190W.

Structurei

3D structure databases

ProteinModelPortaliP29704.
SMRiP29704.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016034.
HOGENOMiHOG000186940.
InParanoidiP29704.
KOiK00801.
OMAiFNFVAIP.
OrthoDBiEOG092C2IU6.

Family and domain databases

CDDicd00683. Trans_IPPS_HH. 1 hit.
Gene3Di1.10.600.10. 1 hit.
InterProiView protein in InterPro
IPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
IPR033904. Trans_IPPS_HH.
PfamiView protein in Pfam
PF00494. SQS_PSY. 1 hit.
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR01559. squal_synth. 1 hit.
PROSITEiView protein in PROSITE
PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P29704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKLLQLALH PVEMKAALKL KFCRTPLFSI YDQSTSPYLL HCFELLNLTS
60 70 80 90 100
RSFAAVIREL HPELRNCVTL FYLILRALDT IEDDMSIEHD LKIDLLRHFH
110 120 130 140 150
EKLLLTKWSF DGNAPDVKDR AVLTDFESIL IEFHKLKPEY QEVIKEITEK
160 170 180 190 200
MGNGMADYIL DENYNLNGLQ TVHDYDVYCH YVAGLVGDGL TRLIVIAKFA
210 220 230 240 250
NESLYSNEQL YESMGLFLQK TNIIRDYNED LVDGRSFWPK EIWSQYAPQL
260 270 280 290 300
KDFMKPENEQ LGLDCINHLV LNALSHVIDV LTYLAGIHEQ STFQFCAIPQ
310 320 330 340 350
VMAIATLALV FNNREVLHGN VKIRKGTTCY LILKSRTLRG CVEIFDYYLR
360 370 380 390 400
DIKSKLAVQD PNFLKLNIQI SKIEQFMEEM YQDKLPPNVK PNETPIFLKV
410 420 430 440
KERSRYDDEL VPTQQEEEYK FNMVLSIILS VLLGFYYIYT LHRA
Length:444
Mass (Da):51,720
Last modified:February 1, 1995 - v2
Checksum:i3DA00295B8DFDB16
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48L → F in CAA42583 (PubMed:1807826).Curated1
Sequence conflicti286G → S in AAA34597 (PubMed:2068081).Curated1
Sequence conflicti320N → D in CAA42583 (PubMed:1807826).Curated1
Sequence conflicti330Y → C in CAA42583 (PubMed:1807826).Curated1
Sequence conflicti429L → S in CAA42583 (PubMed:1807826).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63979 Genomic DNA. Translation: AAA34597.1.
X59959 Genomic DNA. Translation: CAA42583.1.
U00030 Genomic DNA. Translation: AAB68360.1.
BK006934 Genomic DNA. Translation: DAA06882.1.
PIRiS46682.
RefSeqiNP_012060.1. NM_001179321.1.

Genome annotation databases

EnsemblFungiiYHR190W; YHR190W; YHR190W.
GeneIDi856597.
KEGGisce:YHR190W.

Similar proteinsi

Entry informationi

Entry nameiFDFT_YEAST
AccessioniPrimary (citable) accession number: P29704
Secondary accession number(s): D3DLD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1995
Last modified: October 25, 2017
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names