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P29704

- FDFT_YEAST

UniProt

P29704 - FDFT_YEAST

Protein

Squalene synthase

Gene

ERG9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol. May also have a regulatory role regulating the flux of isoprene intermediates through the sterol pathway. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. ERG9 is also essential for cell growth in yeast.3 Publications

    Catalytic activityi

    2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

    Cofactori

    Magnesium.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. farnesyl-diphosphate farnesyltransferase activity Source: SGD
    2. oxidoreductase activity Source: UniProtKB-KW
    3. squalene synthase activity Source: SGD

    GO - Biological processi

    1. ergosterol biosynthetic process Source: SGD
    2. isoprenoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase, Transferase

    Keywords - Biological processi

    Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    Magnesium, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:YHR190W-MONOMER.
    YEAST:YHR190W-MONOMER.
    UniPathwayiUPA00767; UER00751.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Squalene synthase (EC:2.5.1.21)
    Short name:
    SQS
    Short name:
    SS
    Alternative name(s):
    FPP:FPP farnesyltransferase
    Farnesyl-diphosphate farnesyltransferase
    Gene namesi
    Name:ERG9
    Ordered Locus Names:YHR190W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR190w.
    SGDiS000001233. ERG9.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444Squalene synthasePRO_0000067454Add
    BLAST

    Proteomic databases

    MaxQBiP29704.
    PaxDbiP29704.
    PeptideAtlasiP29704.

    Expressioni

    Gene expression databases

    GenevestigatoriP29704.

    Interactioni

    Protein-protein interaction databases

    BioGridi36624. 32 interactions.
    DIPiDIP-4576N.
    IntActiP29704. 7 interactions.
    MINTiMINT-491875.
    STRINGi4932.YHR190W.

    Structurei

    3D structure databases

    ProteinModelPortaliP29704.
    SMRiP29704. Positions 34-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei291 – 31121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei421 – 44121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phytoene/squalene synthase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1562.
    GeneTreeiENSGT00390000016034.
    HOGENOMiHOG000186940.
    KOiK00801.
    OMAiEMRHAVC.
    OrthoDBiEOG7GJ6P5.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR002060. Squ/phyt_synthse.
    IPR006449. Squal_synth.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    TIGRFAMsiTIGR01559. squal_synth. 1 hit.
    PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29704-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKLLQLALH PVEMKAALKL KFCRTPLFSI YDQSTSPYLL HCFELLNLTS    50
    RSFAAVIREL HPELRNCVTL FYLILRALDT IEDDMSIEHD LKIDLLRHFH 100
    EKLLLTKWSF DGNAPDVKDR AVLTDFESIL IEFHKLKPEY QEVIKEITEK 150
    MGNGMADYIL DENYNLNGLQ TVHDYDVYCH YVAGLVGDGL TRLIVIAKFA 200
    NESLYSNEQL YESMGLFLQK TNIIRDYNED LVDGRSFWPK EIWSQYAPQL 250
    KDFMKPENEQ LGLDCINHLV LNALSHVIDV LTYLAGIHEQ STFQFCAIPQ 300
    VMAIATLALV FNNREVLHGN VKIRKGTTCY LILKSRTLRG CVEIFDYYLR 350
    DIKSKLAVQD PNFLKLNIQI SKIEQFMEEM YQDKLPPNVK PNETPIFLKV 400
    KERSRYDDEL VPTQQEEEYK FNMVLSIILS VLLGFYYIYT LHRA 444
    Length:444
    Mass (Da):51,720
    Last modified:February 1, 1995 - v2
    Checksum:i3DA00295B8DFDB16
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti48 – 481L → F in CAA42583. (PubMed:1807826)Curated
    Sequence conflicti286 – 2861G → S in AAA34597. (PubMed:2068081)Curated
    Sequence conflicti320 – 3201N → D in CAA42583. (PubMed:1807826)Curated
    Sequence conflicti330 – 3301Y → C in CAA42583. (PubMed:1807826)Curated
    Sequence conflicti429 – 4291L → S in CAA42583. (PubMed:1807826)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63979 Genomic DNA. Translation: AAA34597.1.
    X59959 Genomic DNA. Translation: CAA42583.1.
    U00030 Genomic DNA. Translation: AAB68360.1.
    BK006934 Genomic DNA. Translation: DAA06882.1.
    PIRiS46682.
    RefSeqiNP_012060.1. NM_001179321.1.

    Genome annotation databases

    EnsemblFungiiYHR190W; YHR190W; YHR190W.
    GeneIDi856597.
    KEGGisce:YHR190W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63979 Genomic DNA. Translation: AAA34597.1 .
    X59959 Genomic DNA. Translation: CAA42583.1 .
    U00030 Genomic DNA. Translation: AAB68360.1 .
    BK006934 Genomic DNA. Translation: DAA06882.1 .
    PIRi S46682.
    RefSeqi NP_012060.1. NM_001179321.1.

    3D structure databases

    ProteinModelPortali P29704.
    SMRi P29704. Positions 34-373.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36624. 32 interactions.
    DIPi DIP-4576N.
    IntActi P29704. 7 interactions.
    MINTi MINT-491875.
    STRINGi 4932.YHR190W.

    Proteomic databases

    MaxQBi P29704.
    PaxDbi P29704.
    PeptideAtlasi P29704.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR190W ; YHR190W ; YHR190W .
    GeneIDi 856597.
    KEGGi sce:YHR190W.

    Organism-specific databases

    CYGDi YHR190w.
    SGDi S000001233. ERG9.

    Phylogenomic databases

    eggNOGi COG1562.
    GeneTreei ENSGT00390000016034.
    HOGENOMi HOG000186940.
    KOi K00801.
    OMAi EMRHAVC.
    OrthoDBi EOG7GJ6P5.

    Enzyme and pathway databases

    UniPathwayi UPA00767 ; UER00751 .
    BioCyci MetaCyc:YHR190W-MONOMER.
    YEAST:YHR190W-MONOMER.

    Miscellaneous databases

    NextBioi 982487.
    PROi P29704.

    Gene expression databases

    Genevestigatori P29704.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR002060. Squ/phyt_synthse.
    IPR006449. Squal_synth.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    TIGRFAMsi TIGR01559. squal_synth. 1 hit.
    PROSITEi PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
    PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and primary structure of the ERG9 gene of Saccharomyces cerevisiae encoding squalene synthetase."
      Fegueur M., Richard L., Charles A.D., Karst F.
      Curr. Genet. 20:365-372(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "Molecular cloning and characterization of the yeast gene for squalene synthetase."
      Jennings S.M., Tsay Y.H., Fisch T.M., Robinson G.W.
      Proc. Natl. Acad. Sci. U.S.A. 88:6038-6042(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Yeast squalene synthase: expression, purification, and characterization of soluble recombinant enzyme."
      Zhang D., Jennings S.M., Robinson G.W., Poulter C.D.
      Arch. Biochem. Biophys. 304:133-143(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, COFACTOR.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFDFT_YEAST
    AccessioniPrimary (citable) accession number: P29704
    Secondary accession number(s): D3DLD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 10800 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3