Squalene synthase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P29704 (FDFT_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 125. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Squalene synthase
Short name=SQS
Short name=SS
EC=2.5.1.21

Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase

Gene names

Name:	ERG9
Ordered Locus Names:	YHR190W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol. May also have a regulatory role regulating the flux of isoprene intermediates through the sterol pathway. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. ERG9 is also essential for cell growth in yeast. Ref.1 Ref.2 Ref.5
Catalytic activity	2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)⁺.
Cofactor	Magnesium. Ref.5
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome Ref.1 Ref.6.
Miscellaneous	Present with 10800 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium NADP
Molecular function	Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	ergosterol biosynthetic process Inferred from mutant phenotype Ref.1 PubMed 200835. Source: SGD isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.6. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from direct assay PubMed 350878. Source: SGD mitochondrial outer membrane Inferred from direct assay PubMed 16407407. Source: SGD
Molecular_function	farnesyl-diphosphate farnesyltransferase activity Inferred from direct assay PubMed 8157649 Ref.5. Source: SGD oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW squalene synthase activity Inferred from direct assay PubMed 8157649 Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 444

444

Squalene synthase

PRO_0000067454

Regions

Transmembrane

291 – 311

Helical; Potential

Transmembrane

421 – 441

Helical; Potential

Experimental info

Sequence conflict

L → F in CAA42583. Ref.1

Sequence conflict

286

G → S in AAA34597. Ref.2

Sequence conflict

320

N → D in CAA42583. Ref.1

Sequence conflict

330

Y → C in CAA42583. Ref.1

Sequence conflict

429

L → S in CAA42583. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P29704 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 3DA00295B8DFDB16
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FASTA

444

51,720

        10         20         30         40         50         60 
MGKLLQLALH PVEMKAALKL KFCRTPLFSI YDQSTSPYLL HCFELLNLTS RSFAAVIREL 

        70         80         90        100        110        120 
HPELRNCVTL FYLILRALDT IEDDMSIEHD LKIDLLRHFH EKLLLTKWSF DGNAPDVKDR 

       130        140        150        160        170        180 
AVLTDFESIL IEFHKLKPEY QEVIKEITEK MGNGMADYIL DENYNLNGLQ TVHDYDVYCH 

       190        200        210        220        230        240 
YVAGLVGDGL TRLIVIAKFA NESLYSNEQL YESMGLFLQK TNIIRDYNED LVDGRSFWPK 

       250        260        270        280        290        300 
EIWSQYAPQL KDFMKPENEQ LGLDCINHLV LNALSHVIDV LTYLAGIHEQ STFQFCAIPQ 

       310        320        330        340        350        360 
VMAIATLALV FNNREVLHGN VKIRKGTTCY LILKSRTLRG CVEIFDYYLR DIKSKLAVQD 

       370        380        390        400        410        420 
PNFLKLNIQI SKIEQFMEEM YQDKLPPNVK PNETPIFLKV KERSRYDDEL VPTQQEEEYK 

       430        440 
FNMVLSIILS VLLGFYYIYT LHRA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and primary structure of the ERG9 gene of Saccharomyces cerevisiae encoding squalene synthetase." Fegueur M., Richard L., Charles A.D., Karst F. Curr. Genet. 20:365-372(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"Molecular cloning and characterization of the yeast gene for squalene synthetase." Jennings S.M., Tsay Y.H., Fisch T.M., Robinson G.W. Proc. Natl. Acad. Sci. U.S.A. 88:6038-6042(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast squalene synthase: expression, purification, and characterization of soluble recombinant enzyme." Zhang D., Jennings S.M., Robinson G.W., Poulter C.D. Arch. Biochem. Biophys. 304:133-143(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, COFACTOR.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63979 Genomic DNA. Translation: AAA34597.1. X59959 Genomic DNA. Translation: CAA42583.1. U00030 Genomic DNA. Translation: AAB68360.1. BK006934 Genomic DNA. Translation: DAA06882.1.
PIR	S46682.
RefSeq	NP_012060.1. NM_001179321.1.
3D structure databases
ProteinModelPortal	P29704.
SMR	P29704. Positions 34-373.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4576N.
IntAct	P29704. 7 interactions.
MINT	MINT-491875.
STRING	4932.YHR190W.
Proteomic databases
PaxDb	P29704.
PeptideAtlas	P29704.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR190W; YHR190W; YHR190W.
GeneID	856597.
KEGG	sce:YHR190W.
Organism-specific databases
CYGD	YHR190w.
SGD	S000001233. ERG9.
Phylogenomic databases
eggNOG	COG1562.
GeneTree	ENSGT00390000016034.
HOGENOM	HOG000186940.
KO	K00801.
OMA	ELRHAVC.
OrthoDB	EOG4BP4MQ.
Enzyme and pathway databases
BioCyc	MetaCyc:YHR190W-MONOMER.
UniPathway	UPA00767; UER00751.
Gene expression databases
ArrayExpress	P29704.
Genevestigator	P29704.
GermOnline	YHR190W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR006449. Squal_synth. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR01559. squal_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982487.

Entry information

Entry name

FDFT_YEAST

Accession

Primary (citable) accession number: P29704
Secondary accession number(s): D3DLD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents