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P29704 (FDFT_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Squalene synthase

Short name=SQS
Short name=SS
EC=2.5.1.21
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene names
Name:ERG9
Ordered Locus Names:YHR190W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol. May also have a regulatory role regulating the flux of isoprene intermediates through the sterol pathway. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. ERG9 is also essential for cell growth in yeast. Ref.1 Ref.2 Ref.5

Catalytic activity

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactor

Magnesium. Ref.5

Pathway

Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome Ref.1 Ref.6.

Miscellaneous

Present with 10800 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the phytoene/squalene synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Squalene synthase
PRO_0000067454

Regions

Transmembrane291 – 31121Helical; Potential
Transmembrane421 – 44121Helical; Potential

Experimental info

Sequence conflict481L → F in CAA42583. Ref.1
Sequence conflict2861G → S in AAA34597. Ref.2
Sequence conflict3201N → D in CAA42583. Ref.1
Sequence conflict3301Y → C in CAA42583. Ref.1
Sequence conflict4291L → S in CAA42583. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P29704 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 3DA00295B8DFDB16

FASTA44451,720
        10         20         30         40         50         60 
MGKLLQLALH PVEMKAALKL KFCRTPLFSI YDQSTSPYLL HCFELLNLTS RSFAAVIREL 

        70         80         90        100        110        120 
HPELRNCVTL FYLILRALDT IEDDMSIEHD LKIDLLRHFH EKLLLTKWSF DGNAPDVKDR 

       130        140        150        160        170        180 
AVLTDFESIL IEFHKLKPEY QEVIKEITEK MGNGMADYIL DENYNLNGLQ TVHDYDVYCH 

       190        200        210        220        230        240 
YVAGLVGDGL TRLIVIAKFA NESLYSNEQL YESMGLFLQK TNIIRDYNED LVDGRSFWPK 

       250        260        270        280        290        300 
EIWSQYAPQL KDFMKPENEQ LGLDCINHLV LNALSHVIDV LTYLAGIHEQ STFQFCAIPQ 

       310        320        330        340        350        360 
VMAIATLALV FNNREVLHGN VKIRKGTTCY LILKSRTLRG CVEIFDYYLR DIKSKLAVQD 

       370        380        390        400        410        420 
PNFLKLNIQI SKIEQFMEEM YQDKLPPNVK PNETPIFLKV KERSRYDDEL VPTQQEEEYK 

       430        440 
FNMVLSIILS VLLGFYYIYT LHRA 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and primary structure of the ERG9 gene of Saccharomyces cerevisiae encoding squalene synthetase."
Fegueur M., Richard L., Charles A.D., Karst F.
Curr. Genet. 20:365-372(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"Molecular cloning and characterization of the yeast gene for squalene synthetase."
Jennings S.M., Tsay Y.H., Fisch T.M., Robinson G.W.
Proc. Natl. Acad. Sci. U.S.A. 88:6038-6042(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Yeast squalene synthase: expression, purification, and characterization of soluble recombinant enzyme."
Zhang D., Jennings S.M., Robinson G.W., Poulter C.D.
Arch. Biochem. Biophys. 304:133-143(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COFACTOR.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63979 Genomic DNA. Translation: AAA34597.1.
X59959 Genomic DNA. Translation: CAA42583.1.
U00030 Genomic DNA. Translation: AAB68360.1.
BK006934 Genomic DNA. Translation: DAA06882.1.
PIRS46682.
RefSeqNP_012060.1. NM_001179321.1.

3D structure databases

ProteinModelPortalP29704.
SMRP29704. Positions 34-373.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36624. 32 interactions.
DIPDIP-4576N.
IntActP29704. 7 interactions.
MINTMINT-491875.
STRING4932.YHR190W.

Proteomic databases

PaxDbP29704.
PeptideAtlasP29704.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR190W; YHR190W; YHR190W.
GeneID856597.
KEGGsce:YHR190W.

Organism-specific databases

CYGDYHR190w.
SGDS000001233. ERG9.

Phylogenomic databases

eggNOGCOG1562.
GeneTreeENSGT00390000016034.
HOGENOMHOG000186940.
KOK00801.
OMAEMRHAVC.
OrthoDBEOG7GJ6P5.

Enzyme and pathway databases

BioCycMetaCyc:YHR190W-MONOMER.
YEAST:YHR190W-MONOMER.
UniPathwayUPA00767; UER00751.

Gene expression databases

GenevestigatorP29704.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR002060. Squ/phyt_synthse.
IPR006449. Squal_synth.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
TIGRFAMsTIGR01559. squal_synth. 1 hit.
PROSITEPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982487.
PROP29704.

Entry information

Entry nameFDFT_YEAST
AccessionPrimary (citable) accession number: P29704
Secondary accession number(s): D3DLD8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways