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Protein

Alpha-2-HS-glycoprotein

Gene

Ahsg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in differentiation.

GO - Molecular functioni

  1. cysteine-type endopeptidase inhibitor activity Source: InterPro

GO - Biological processi

  1. acute-phase response Source: UniProtKB
  2. negative regulation of bone mineralization Source: UniProtKB
  3. ossification Source: MGI
  4. positive regulation of phagocytosis Source: UniProtKB
  5. regulation of inflammatory response Source: UniProtKB
Complete GO annotation...

Protein family/group databases

MEROPSiI25.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-HS-glycoprotein
Alternative name(s):
Countertrypin
Fetuin-A
Gene namesi
Name:Ahsg
Synonyms:Fetua
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:107189. Ahsg.

Subcellular locationi

GO - Cellular componenti

  1. blood microparticle Source: MGI
  2. extracellular space Source: MGI
  3. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 345327Alpha-2-HS-glycoproteinPRO_0000008891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 336PROSITE-ProRule annotation
Disulfide bondi89 ↔ 100PROSITE-ProRule annotation
Glycosylationi99 – 991N-linked (GlcNAc...)1 Publication
Disulfide bondi114 ↔ 132PROSITE-ProRule annotation
Modified residuei134 – 1341PhosphoserineBy similarity
Modified residuei138 – 1381Phosphoserine2 Publications
Disulfide bondi146 ↔ 149PROSITE-ProRule annotation
Glycosylationi156 – 1561N-linked (GlcNAc...)1 Publication
Glycosylationi176 – 1761N-linked (GlcNAc...)2 Publications
Disulfide bondi208 ↔ 219PROSITE-ProRule annotation
Disulfide bondi230 ↔ 247PROSITE-ProRule annotation
Modified residuei309 – 3091Phosphoserine2 Publications
Modified residuei312 – 3121Phosphoserine2 Publications
Modified residuei314 – 3141Phosphoserine2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP29699.
PaxDbiP29699.
PRIDEiP29699.

2D gel databases

REPRODUCTION-2DPAGEIPI00128249.

PTM databases

PhosphoSiteiP29699.

Expressioni

Tissue specificityi

Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development.

Gene expression databases

BgeeiP29699.
CleanExiMM_AHSG.
ExpressionAtlasiP29699. baseline and differential.
GenevestigatoriP29699.

Interactioni

Protein-protein interaction databases

BioGridi198039. 1 interaction.
IntActiP29699. 8 interactions.
MINTiMINT-1846156.

Structurei

3D structure databases

ProteinModelPortaliP29699.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 133115Cystatin fetuin-A-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini144 – 250107Cystatin fetuin-A-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the fetuin family.PROSITE-ProRule annotation
Contains 2 cystatin fetuin-A-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG44208.
HOGENOMiHOG000290189.
HOVERGENiHBG051607.
InParanoidiP29699.
OMAiRAHYDLR.
OrthoDBiEOG71K63R.
PhylomeDBiP29699.
TreeFamiTF333729.

Family and domain databases

InterProiIPR025760. Cystatin_Fetuin_A.
IPR000010. Prot_inh_cystat.
IPR001363. Prot_inh_fetuin_CS.
[Graphical view]
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 2 hits.
[Graphical view]
PROSITEiPS51529. CYSTATIN_FETUIN_A. 2 hits.
PS01254. FETUIN_1. 1 hit.
PS01255. FETUIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLVLLLCF AQLWGCQSAP QGTGLGFREL ACDDPEAEQV ALLAVDYLNN
60 70 80 90 100
HLLQGFKQVL NQIDKVKVWS RRPFGVVYEM EVDTLETTCH ALDPTPLANC
110 120 130 140 150
SVRQLTEHAV EGDCDFHILK QDGQFRVMHT QCHSTPDSAE DVRKLCPRCP
160 170 180 190 200
LLTPFNDTNV VHTVNTALAA FNTQNNGTYF KLVEISRAQN VPLPVSTLVE
210 220 230 240 250
FVIAATDCTA KEVTDPAKCN LLAEKQHGFC KANLMHNLGG EEVSVACKLF
260 270 280 290 300
QTQPQPANAN AVGPVPTANA ALPADPPASV VVGPVVVPRG LSDHRTYHDL
310 320 330 340
RHAFSPVASV ESASGETLHS PKVGQPGAAG PVSPMCPGRI RHFKI
Length:345
Mass (Da):37,326
Last modified:April 1, 1993 - v1
Checksum:i4B7B9C9B1410658E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711R → RQ in CAA05210 (PubMed:9395485).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S96534 mRNA. Translation: AAB22070.1.
AF007900 Genomic DNA. Translation: AAB81718.1.
AJ002146 Genomic DNA. Translation: CAA05210.1.
BC012678 mRNA. Translation: AAH12678.1.
BC019822 mRNA. Translation: AAH19822.1.
CCDSiCCDS28071.1.
PIRiS21094.
RefSeqiNP_038493.1. NM_013465.2.
UniGeneiMm.197554.

Genome annotation databases

EnsembliENSMUST00000023583; ENSMUSP00000023583; ENSMUSG00000022868.
GeneIDi11625.
KEGGimmu:11625.
UCSCiuc007ysr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S96534 mRNA. Translation: AAB22070.1.
AF007900 Genomic DNA. Translation: AAB81718.1.
AJ002146 Genomic DNA. Translation: CAA05210.1.
BC012678 mRNA. Translation: AAH12678.1.
BC019822 mRNA. Translation: AAH19822.1.
CCDSiCCDS28071.1.
PIRiS21094.
RefSeqiNP_038493.1. NM_013465.2.
UniGeneiMm.197554.

3D structure databases

ProteinModelPortaliP29699.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198039. 1 interaction.
IntActiP29699. 8 interactions.
MINTiMINT-1846156.

Protein family/group databases

MEROPSiI25.020.

PTM databases

PhosphoSiteiP29699.

2D gel databases

REPRODUCTION-2DPAGEIPI00128249.

Proteomic databases

MaxQBiP29699.
PaxDbiP29699.
PRIDEiP29699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023583; ENSMUSP00000023583; ENSMUSG00000022868.
GeneIDi11625.
KEGGimmu:11625.
UCSCiuc007ysr.1. mouse.

Organism-specific databases

CTDi197.
MGIiMGI:107189. Ahsg.

Phylogenomic databases

eggNOGiNOG44208.
HOGENOMiHOG000290189.
HOVERGENiHBG051607.
InParanoidiP29699.
OMAiRAHYDLR.
OrthoDBiEOG71K63R.
PhylomeDBiP29699.
TreeFamiTF333729.

Miscellaneous databases

NextBioi279179.
PROiP29699.
SOURCEiSearch...

Gene expression databases

BgeeiP29699.
CleanExiMM_AHSG.
ExpressionAtlasiP29699. baseline and differential.
GenevestigatoriP29699.

Family and domain databases

InterProiIPR025760. Cystatin_Fetuin_A.
IPR000010. Prot_inh_cystat.
IPR001363. Prot_inh_fetuin_CS.
[Graphical view]
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 2 hits.
[Graphical view]
PROSITEiPS51529. CYSTATIN_FETUIN_A. 2 hits.
PS01254. FETUIN_1. 1 hit.
PS01255. FETUIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human alpha 2-HS-glycoprotein/bovine fetuin homologue in mice: identification and developmental regulation of the gene."
    Yang F., Chen Z.-L., Bergeron J.M., Cupples R.L., Friedrichs W.E.
    Biochim. Biophys. Acta 1130:149-156(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver and Salivary gland.
  4. "Isolation and characterization of mouse countertrypin, a new trypsin inhibitor belonging to the mammalian fetuin family."
    Yamamoto K., Sinohara H.
    J. Biol. Chem. 268:17750-17753(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
  5. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-176.
    Strain: C57BL/6.
    Tissue: Plasma.
  6. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-99; ASN-156 AND ASN-176.
    Strain: C57BL/6.
    Tissue: Plasma.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138; SER-309; SER-312 AND SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138; SER-309; SER-312 AND SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiFETUA_MOUSE
AccessioniPrimary (citable) accession number: P29699
Secondary accession number(s): O35634
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: March 4, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.