Alpha-2-HS-glycoprotein precursor - Mus musculus (Mouse)

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P29699 (FETUA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-2-HS-glycoprotein

Alternative name(s):
Countertrypin
Fetuin-A

Gene names

Name:	Ahsg
Synonyms:	Fetua

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in differentiation.
Subcellular location	Secreted.
Tissue specificity	Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development.
Sequence similarities	Belongs to the fetuin family. Contains 2 cystatin fetuin-A-type domains.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	acute-phase response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of bone mineralization Inferred from direct assay PubMed 12556469. Source: UniProtKB ossification Inferred from direct assay PubMed 8695840. Source: MGI positive regulation of phagocytosis Inferred from sequence or structural similarity. Source: UniProtKB regulation of inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	extracellular space Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

Potential

Chain

19 – 345

327

Alpha-2-HS-glycoprotein

PRO_0000008891

Regions

Domain

19 – 133

115

Cystatin fetuin-A-type 1

Domain

144 – 250

107

Cystatin fetuin-A-type 2

Amino acid modifications

Modified residue

138

Phosphoserine Ref.8 Ref.9

Modified residue

305

Phosphoserine Ref.7

Modified residue

309

Phosphoserine Ref.7 Ref.9

Modified residue

312

Phosphoserine Ref.7 Ref.9

Modified residue

314

Phosphoserine Ref.7

Glycosylation

N-linked (GlcNAc...) Ref.6

Glycosylation

156

N-linked (GlcNAc...) Ref.6

Glycosylation

176

N-linked (GlcNAc...) Ref.5 Ref.6

Disulfide bond

32 ↔ 336

By similarity

Disulfide bond

89 ↔ 100

By similarity

Disulfide bond

114 ↔ 132

By similarity

Disulfide bond

146 ↔ 149

By similarity

Disulfide bond

208 ↔ 219

By similarity

Disulfide bond

230 ↔ 247

By similarity

Experimental info

Sequence conflict

R → RQ in CAA05210. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P29699 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 4B7B9C9B1410658E
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FASTA

345

37,326

        10         20         30         40         50         60 
MKSLVLLLCF AQLWGCQSAP QGTGLGFREL ACDDPEAEQV ALLAVDYLNN HLLQGFKQVL 

        70         80         90        100        110        120 
NQIDKVKVWS RRPFGVVYEM EVDTLETTCH ALDPTPLANC SVRQLTEHAV EGDCDFHILK 

       130        140        150        160        170        180 
QDGQFRVMHT QCHSTPDSAE DVRKLCPRCP LLTPFNDTNV VHTVNTALAA FNTQNNGTYF 

       190        200        210        220        230        240 
KLVEISRAQN VPLPVSTLVE FVIAATDCTA KEVTDPAKCN LLAEKQHGFC KANLMHNLGG 

       250        260        270        280        290        300 
EEVSVACKLF QTQPQPANAN AVGPVPTANA ALPADPPASV VVGPVVVPRG LSDHRTYHDL 

       310        320        330        340 
RHAFSPVASV ESASGETLHS PKVGQPGAAG PVSPMCPGRI RHFKI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human alpha 2-HS-glycoprotein/bovine fetuin homologue in mice: identification and developmental regulation of the gene." Yang F., Chen Z.-L., Bergeron J.M., Cupples R.L., Friedrichs W.E. Biochim. Biophys. Acta 1130:149-156(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Liver.
[2]	"Cloning and targeted deletion of the mouse fetuin gene." Jahnen-Dechent W., Schinke T., Trindl A., Mueller-Esterl W., Sablitzky F., Kaiser S., Blessing M. J. Biol. Chem. 272:31496-31503(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver and Salivary gland.
[4]	"Isolation and characterization of mouse countertrypin, a new trypsin inhibitor belonging to the mammalian fetuin family." Yamamoto K., Sinohara H. J. Biol. Chem. 268:17750-17753(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[5]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-176, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
[6]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-99; ASN-156 AND ASN-176, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
[7]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305; SER-309; SER-312 AND SER-314, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, MASS SPECTROMETRY. Tissue: Liver.
[9]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138; SER-309 AND SER-312, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S96534 mRNA. Translation: AAB22070.1. AF007900 Genomic DNA. Translation: AAB81718.1. AJ002146 Genomic DNA. Translation: CAA05210.1. BC012678 mRNA. Translation: AAH12678.1. BC019822 mRNA. Translation: AAH19822.1.
IPI	IPI00128249.
PIR	S21094.
RefSeq	NP_038493.1. NM_013465.2.
UniGene	Mm.197554.
3D structure databases
ProteinModelPortal	P29699.
SMR	P29699. Positions 34-89.
ModBase	Search...
Protein-protein interaction databases
IntAct	P29699. 3 interactions.
Protein family/group databases
MEROPS	I25.020.
PTM databases
PhosphoSite	P29699.
2D gel databases
REPRODUCTION-2DPAGE	IPI00128249.
Proteomic databases
PaxDb	P29699.
PRIDE	P29699.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023583; ENSMUSP00000023583; ENSMUSG00000022868.
GeneID	11625.
KEGG	mmu:11625.
Organism-specific databases
CTD	197.
MGI	MGI:107189. Ahsg.
Phylogenomic databases
eggNOG	NOG44208.
HOGENOM	HOG000290189.
HOVERGEN	HBG051607.
InParanoid	P29699.
OMA	RAHYDLR.
OrthoDB	EOG4QC164.
Gene expression databases
ArrayExpress	P29699.
Bgee	P29699.
CleanEx	MM_AHSG.
Genevestigator	P29699.
GermOnline	ENSMUSG00000022868. Mus musculus.
Family and domain databases
InterPro	IPR025760. Cystatin_Fetuin_A. IPR000010. Prot_inh_cystat. IPR001363. Prot_inh_fetuin_CS. [Graphical view]
Pfam	PF00031. Cystatin. 1 hit. [Graphical view]
SMART	SM00043. CY. 2 hits. [Graphical view]
PROSITE	PS51529. CYSTATIN_FETUIN_A. 2 hits. PS01254. FETUIN_1. 1 hit. PS01255. FETUIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279179.
SOURCE	Search...

Entry information

Entry name

FETUA_MOUSE

Accession

Primary (citable) accession number: P29699
Secondary accession number(s): O35634

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents