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Protein

Elongation factor 2

Gene

eef-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Involved in the morphogenesis of epidermal tissues.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338GTPBy similarity
Nucleotide bindingi116 – 1205GTPBy similarity
Nucleotide bindingi170 – 1734GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-156902. Peptide chain elongation.
R-CEL-5358493. Synthesis of diphthamide-EEF2.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:eef-2
ORF Names:F25H5.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF25H5.4a; CE15900; WBGene00001167; eef-2.
F25H5.4b; CE46149; WBGene00001167; eef-2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Ray defect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 852851Elongation factor 2PRO_0000091008Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571PhosphothreonineBy similarity
Modified residuei59 – 591PhosphothreonineBy similarity
Modified residuei709 – 7091DiphthamideBy similarity

Post-translational modificationi

Phosphorylation by EF-2 kinase completely inactivates EF-2.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP29691.
PaxDbiP29691.
PRIDEiP29691.

2D gel databases

World-2DPAGE0020:P29691.

PTM databases

iPTMnetiP29691.

Interactioni

Protein-protein interaction databases

BioGridi38174. 4 interactions.
DIPiDIP-26688N.
IntActiP29691. 5 interactions.
MINTiMINT-228785.
STRINGi6239.F25H5.4a.2.

Structurei

3D structure databases

ProteinModelPortaliP29691.
SMRiP29691. Positions 2-852.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 356340tr-type GPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0469. Eukaryota.
COG0480. LUCA.
GeneTreeiENSGT00840000129841.
HOGENOMiHOG000231589.
InParanoidiP29691.
KOiK03234.
OMAiFTGHVTR.
PhylomeDBiP29691.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P29691-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVNFTVDEIR ALMDRKRNIR NMSVIAHVDH GKSTLTDSLV SKAGIIAGSK
60 70 80 90 100
AGETRFTDTR KDEQERCITI KSTAISLFFE LEKKDLEFVK GENQFETVEV
110 120 130 140 150
DGKKEKYNGF LINLIDSPGH VDFSSEVTAA LRVTDGALVV VDCVSGVCVQ
160 170 180 190 200
TETVLRQAIA ERIKPVLFMN KMDRALLELQ LGAEELFQTF QRIVENINVI
210 220 230 240 250
IATYGDDDGP MGPIMVDPSI GNVGFGSGLH GWAFTLKQFA EMYAGKFGVQ
260 270 280 290 300
VDKLMKNLWG DRFFDLKTKK WSSTQTDESK RGFCQFVLDP IFMVFDAVMN
310 320 330 340 350
IKKDKTAALV EKLGIKLAND EKDLEGKPLM KVFMRKWLPA GDTMLQMIAF
360 370 380 390 400
HLPSPVTAQK YRMEMLYEGP HDDEAAVAIK TCDPNGPLMM YISKMVPTSD
410 420 430 440 450
KGRFYAFGRV FSGKVATGMK ARIQGPNYVP GKKEDLYEKT IQRTILMMGR
460 470 480 490 500
FIEPIEDIPS GNIAGLVGVD QYLVKGGTIT TYKDAHNMRV MKFSVSPVVR
510 520 530 540 550
VAVEAKNPAD LPKLVEGLKR LAKSDPMVQC IFEESGEHII AGAGELHLEI
560 570 580 590 600
CLKDLEEDHA CIPLKKSDPV VSYRETVQSE SNQICLSKSP NKHNRLHCTA
610 620 630 640 650
QPMPDGLADD IEGGTVNARD EFKARAKILA EKYEYDVTEA RKIWCFGPDG
660 670 680 690 700
TGPNLLMDVT KGVQYLNEIK DSVVAGFQWA TREGVLSDEN MRGVRFNVHD
710 720 730 740 750
VTLHADAIHR GGGQIIPTAR RVFYASVLTA EPRLLEPVYL VEIQCPEAAV
760 770 780 790 800
GGIYGVLNRR RGHVFEESQV TGTPMFVVKA YLPVNESFGF TADLRSNTGG
810 820 830 840 850
QAFPQCVFDH WQVLPGDPLE AGTKPNQIVL DTRKRKGLKE GVPALDNYLD

KM
Length:852
Mass (Da):94,796
Last modified:January 23, 2007 - v4
Checksum:i849676A861DBC4C4
GO
Isoform b (identifier: P29691-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: Missing.

Note: No experimental confirmation available.
Show »
Length:840
Mass (Da):93,407
Checksum:i17D93EA5D98D7B2E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321R → G in AAD03339 (PubMed:1930695).Curated
Sequence conflicti617 – 6171N → S in AAD03339 (PubMed:1930695).Curated
Sequence conflicti628 – 6303ILA → YPG in AAD03339 (PubMed:1930695).Curated
Sequence conflicti636 – 6361D → A in AAD03339 (PubMed:1930695).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1212Missing in isoform b. CuratedVSP_044101Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86959 mRNA. Translation: AAD03339.1.
Z81068 Genomic DNA. Translation: CAB02985.1.
Z81068 Genomic DNA. Translation: CCD31064.1.
PIRiA40411.
T21362.
RefSeqiNP_001251010.1. NM_001264081.1. [P29691-1]
NP_001251011.1. NM_001264082.1. [P29691-2]
UniGeneiCel.38673.

Genome annotation databases

EnsemblMetazoaiF25H5.4a.1; F25H5.4a.1; WBGene00001167. [P29691-1]
F25H5.4a.2; F25H5.4a.2; WBGene00001167. [P29691-1]
GeneIDi172743.
KEGGicel:CELE_F25H5.4.
UCSCiF25H5.4.2. c. elegans. [P29691-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86959 mRNA. Translation: AAD03339.1.
Z81068 Genomic DNA. Translation: CAB02985.1.
Z81068 Genomic DNA. Translation: CCD31064.1.
PIRiA40411.
T21362.
RefSeqiNP_001251010.1. NM_001264081.1. [P29691-1]
NP_001251011.1. NM_001264082.1. [P29691-2]
UniGeneiCel.38673.

3D structure databases

ProteinModelPortaliP29691.
SMRiP29691. Positions 2-852.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi38174. 4 interactions.
DIPiDIP-26688N.
IntActiP29691. 5 interactions.
MINTiMINT-228785.
STRINGi6239.F25H5.4a.2.

PTM databases

iPTMnetiP29691.

2D gel databases

World-2DPAGE0020:P29691.

Proteomic databases

EPDiP29691.
PaxDbiP29691.
PRIDEiP29691.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF25H5.4a.1; F25H5.4a.1; WBGene00001167. [P29691-1]
F25H5.4a.2; F25H5.4a.2; WBGene00001167. [P29691-1]
GeneIDi172743.
KEGGicel:CELE_F25H5.4.
UCSCiF25H5.4.2. c. elegans. [P29691-1]

Organism-specific databases

CTDi172743.
WormBaseiF25H5.4a; CE15900; WBGene00001167; eef-2.
F25H5.4b; CE46149; WBGene00001167; eef-2.

Phylogenomic databases

eggNOGiKOG0469. Eukaryota.
COG0480. LUCA.
GeneTreeiENSGT00840000129841.
HOGENOMiHOG000231589.
InParanoidiP29691.
KOiK03234.
OMAiFTGHVTR.
PhylomeDBiP29691.

Enzyme and pathway databases

ReactomeiR-CEL-156902. Peptide chain elongation.
R-CEL-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

PROiP29691.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the Caenorhabditis elegans elongation factor 2 gene (eft-2)."
    Ofulue E.N., Candido E.P.M.
    DNA Cell Biol. 10:603-611(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Semaphorin controls epidermal morphogenesis by stimulating mRNA translation via eIF2alpha in Caenorhabditis elegans."
    Nukazuka A., Fujisawa H., Inada T., Oda Y., Takagi S.
    Genes Dev. 22:1025-1036(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiEF2_CAEEL
AccessioniPrimary (citable) accession number: P29691
Secondary accession number(s): G3MU53, O17837
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 137 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.