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Protein

Uroporphyrinogen decarboxylase

Gene

hemE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.UniRule annotation

Catalytic activityi

Uroporphyrinogen III = coproporphyrinogen + 4 CO2.UniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. Uroporphyrinogen-III synthase (hemD)
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46SubstrateUniRule annotation1
Binding sitei77SubstrateUniRule annotation1
Sitei77Transition state stabilizerUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Binding sitei209SubstrateUniRule annotation1
Binding sitei327SubstrateUniRule annotation1

GO - Molecular functioni

  • uroporphyrinogen decarboxylase activity Source: EcoCyc

GO - Biological processi

  • heme biosynthetic process Source: EcoliWiki
  • porphyrin-containing compound biosynthetic process Source: EcoliWiki
  • protoporphyrinogen IX biosynthetic process from glutamate Source: EcoCyc
  • uroporphyrinogen III biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:UROGENDECARBOX-MONOMER.
ECOL316407:JW3961-MONOMER.
MetaCyc:UROGENDECARBOX-MONOMER.
UniPathwayiUPA00251; UER00321.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen decarboxylaseUniRule annotation (EC:4.1.1.37UniRule annotation)
Short name:
UPDUniRule annotation
Short name:
URO-DUniRule annotation
Gene namesi
Name:hemEUniRule annotation
Ordered Locus Names:b3997, JW3961
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11543. hemE.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001876031 – 354Uroporphyrinogen decarboxylaseAdd BLAST354

Proteomic databases

PaxDbiP29680.
PRIDEiP29680.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP29680. 5 interactors.
STRINGi511145.b3997.

Structurei

3D structure databases

ProteinModelPortaliP29680.
SMRiP29680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 31Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the uroporphyrinogen decarboxylase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CFZ. Bacteria.
COG0407. LUCA.
HOGENOMiHOG000253896.
InParanoidiP29680.
KOiK01599.
OMAiGSSKDFR.
PhylomeDBiP29680.

Family and domain databases

CDDicd00717. URO-D. 1 hit.
HAMAPiMF_00218. URO_D. 1 hit.
InterProiIPR006361. Uroporphyrinogen_deCO2ase_HemE.
IPR000257. Uroporphyrinogen_deCOase.
[Graphical view]
PfamiPF01208. URO-D. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01464. hemE. 1 hit.
PROSITEiPS00906. UROD_1. 1 hit.
PS00907. UROD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELKNDRYL RALLRQPVDV TPVWMMRQAG RYLPEYKATR AQAGDFMSLC
60 70 80 90 100
KNAELACEVT LQPLRRYPLD AAILFSDILT VPDAMGLGLY FEAGEGPRFT
110 120 130 140 150
SPVTCKADVD KLPIPDPEDE LGYVMNAVRT IRRELKGEVP LIGFSGSPWT
160 170 180 190 200
LATYMVEGGS SKAFTVIKKM MYADPQALHA LLDKLAKSVT LYLNAQIKAG
210 220 230 240 250
AQAVMIFDTW GGVLTGRDYQ QFSLYYMHKI VDGLLRENDG RRVPVTLFTK
260 270 280 290 300
GGGQWLEAMA ETGCDALGLD WTTDIADARR RVGNKVALQG NMDPSMLYAP
310 320 330 340 350
PARIEEEVAT ILAGFGHGEG HVFNLGHGIH QDVPPEHAGV FVEAVHRLSE

QYHR
Length:354
Mass (Da):39,248
Last modified:February 1, 1996 - v3
Checksum:i604933C50080260C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72 – 78AILFSDI → RSSFRY in BAA02148 (PubMed:8224882).Curated7
Sequence conflicti83D → I in BAA02148 (PubMed:8224882).Curated1
Sequence conflicti89 – 103LYFEA…FTSPV → SSILKPEKVRVLPRQI in BAA02148 (PubMed:8224882).CuratedAdd BLAST15
Sequence conflicti251 – 256GGGQWL → SATVA in BAA02148 (PubMed:8224882).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12624 Genomic DNA. Translation: BAA02148.1.
U00006 Genomic DNA. Translation: AAC43095.1.
U00096 Genomic DNA. Translation: AAC76971.1.
AP009048 Genomic DNA. Translation: BAE77322.1.
PIRiH65206.
RefSeqiNP_418425.1. NC_000913.3.
WP_000137657.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76971; AAC76971; b3997.
BAE77322; BAE77322; BAE77322.
GeneIDi948497.
KEGGiecj:JW3961.
eco:b3997.
PATRICi32123517. VBIEscCol129921_4111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12624 Genomic DNA. Translation: BAA02148.1.
U00006 Genomic DNA. Translation: AAC43095.1.
U00096 Genomic DNA. Translation: AAC76971.1.
AP009048 Genomic DNA. Translation: BAE77322.1.
PIRiH65206.
RefSeqiNP_418425.1. NC_000913.3.
WP_000137657.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP29680.
SMRiP29680.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP29680. 5 interactors.
STRINGi511145.b3997.

Proteomic databases

PaxDbiP29680.
PRIDEiP29680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76971; AAC76971; b3997.
BAE77322; BAE77322; BAE77322.
GeneIDi948497.
KEGGiecj:JW3961.
eco:b3997.
PATRICi32123517. VBIEscCol129921_4111.

Organism-specific databases

EchoBASEiEB1505.
EcoGeneiEG11543. hemE.

Phylogenomic databases

eggNOGiENOG4105CFZ. Bacteria.
COG0407. LUCA.
HOGENOMiHOG000253896.
InParanoidiP29680.
KOiK01599.
OMAiGSSKDFR.
PhylomeDBiP29680.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00321.
BioCyciEcoCyc:UROGENDECARBOX-MONOMER.
ECOL316407:JW3961-MONOMER.
MetaCyc:UROGENDECARBOX-MONOMER.

Miscellaneous databases

PROiP29680.

Family and domain databases

CDDicd00717. URO-D. 1 hit.
HAMAPiMF_00218. URO_D. 1 hit.
InterProiIPR006361. Uroporphyrinogen_deCO2ase_HemE.
IPR000257. Uroporphyrinogen_deCOase.
[Graphical view]
PfamiPF01208. URO-D. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01464. hemE. 1 hit.
PROSITEiPS00906. UROD_1. 1 hit.
PS00907. UROD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCUP_ECOLI
AccessioniPrimary (citable) accession number: P29680
Secondary accession number(s): P78135, Q2M8T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.