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Protein

Uroporphyrinogen decarboxylase

Gene

hemE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.UniRule annotation

Catalytic activityi

Uroporphyrinogen III = coproporphyrinogen + 4 CO2.UniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. Uroporphyrinogen-III synthase (hemD)
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46SubstrateUniRule annotation1
Binding sitei77SubstrateUniRule annotation1
Sitei77Transition state stabilizerUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Binding sitei209SubstrateUniRule annotation1
Binding sitei327SubstrateUniRule annotation1

GO - Molecular functioni

  • uroporphyrinogen decarboxylase activity Source: EcoCyc

GO - Biological processi

  • heme biosynthetic process Source: EcoliWiki
  • porphyrin-containing compound biosynthetic process Source: EcoliWiki
  • protoporphyrinogen IX biosynthetic process from glutamate Source: EcoCyc
  • uroporphyrinogen III biosynthetic process Source: EcoliWiki

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processPorphyrin biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:UROGENDECARBOX-MONOMER
MetaCyc:UROGENDECARBOX-MONOMER
UniPathwayiUPA00251; UER00321

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen decarboxylaseUniRule annotation (EC:4.1.1.37UniRule annotation)
Short name:
UPDUniRule annotation
Short name:
URO-DUniRule annotation
Gene namesi
Name:hemEUniRule annotation
Ordered Locus Names:b3997, JW3961
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11543 hemE

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001876031 – 354Uroporphyrinogen decarboxylaseAdd BLAST354

Proteomic databases

PaxDbiP29680
PRIDEiP29680

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridi4263218, 2 interactors
IntActiP29680, 5 interactors
STRINGi316385.ECDH10B_4186

Structurei

3D structure databases

ProteinModelPortaliP29680
SMRiP29680
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 31Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the uroporphyrinogen decarboxylase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CFZ Bacteria
COG0407 LUCA
HOGENOMiHOG000253896
InParanoidiP29680
KOiK01599
OMAiSWAGQLS
PhylomeDBiP29680

Family and domain databases

CDDicd00717 URO-D, 1 hit
Gene3Di3.20.20.210, 1 hit
HAMAPiMF_00218 URO_D, 1 hit
InterProiView protein in InterPro
IPR038071 UROD/MetE-like_sf
IPR006361 Uroporphyrinogen_deCO2ase_HemE
IPR000257 Uroporphyrinogen_deCOase
PfamiView protein in Pfam
PF01208 URO-D, 1 hit
SUPFAMiSSF51726 SSF51726, 1 hit
TIGRFAMsiTIGR01464 hemE, 1 hit
PROSITEiView protein in PROSITE
PS00906 UROD_1, 1 hit
PS00907 UROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P29680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELKNDRYL RALLRQPVDV TPVWMMRQAG RYLPEYKATR AQAGDFMSLC
60 70 80 90 100
KNAELACEVT LQPLRRYPLD AAILFSDILT VPDAMGLGLY FEAGEGPRFT
110 120 130 140 150
SPVTCKADVD KLPIPDPEDE LGYVMNAVRT IRRELKGEVP LIGFSGSPWT
160 170 180 190 200
LATYMVEGGS SKAFTVIKKM MYADPQALHA LLDKLAKSVT LYLNAQIKAG
210 220 230 240 250
AQAVMIFDTW GGVLTGRDYQ QFSLYYMHKI VDGLLRENDG RRVPVTLFTK
260 270 280 290 300
GGGQWLEAMA ETGCDALGLD WTTDIADARR RVGNKVALQG NMDPSMLYAP
310 320 330 340 350
PARIEEEVAT ILAGFGHGEG HVFNLGHGIH QDVPPEHAGV FVEAVHRLSE

QYHR
Length:354
Mass (Da):39,248
Last modified:February 1, 1996 - v3
Checksum:i604933C50080260C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72 – 78AILFSDI → RSSFRY in BAA02148 (PubMed:8224882).Curated7
Sequence conflicti83D → I in BAA02148 (PubMed:8224882).Curated1
Sequence conflicti89 – 103LYFEA…FTSPV → SSILKPEKVRVLPRQI in BAA02148 (PubMed:8224882).CuratedAdd BLAST15
Sequence conflicti251 – 256GGGQWL → SATVA in BAA02148 (PubMed:8224882).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12624 Genomic DNA Translation: BAA02148.1
U00006 Genomic DNA Translation: AAC43095.1
U00096 Genomic DNA Translation: AAC76971.1
AP009048 Genomic DNA Translation: BAE77322.1
PIRiH65206
RefSeqiNP_418425.1, NC_000913.3
WP_000137657.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76971; AAC76971; b3997
BAE77322; BAE77322; BAE77322
GeneIDi948497
KEGGiecj:JW3961
eco:b3997
PATRICifig|1411691.4.peg.2714

Similar proteinsi

Entry informationi

Entry nameiDCUP_ECOLI
AccessioniPrimary (citable) accession number: P29680
Secondary accession number(s): P78135, Q2M8T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health