P29680 (DCUP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uroporphyrinogen decarboxylase
Short name=UPD
Short name=URO-D
EC=4.1.1.37

Gene names

Name:	hemE
Ordered Locus Names:	b3997, JW3961

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III By similarity. HAMAP-Rule MF_00218
Catalytic activity	Uroporphyrinogen III = coproporphyrinogen + 4 CO2. HAMAP-Rule MF_00218
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4. HAMAP-Rule MF_00218
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the uroporphyrinogen decarboxylase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protoporphyrinogen IX biosynthetic process from glutamate Inferred from mutant phenotype PubMed 1104578. Source: EcoCyc uroporphyrinogen III biosynthetic process Inferred from mutant phenotype PubMed 1104578. Source: EcoliWiki
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	uroporphyrinogen decarboxylase activity Inferred from mutant phenotype PubMed 1104578PubMed 7849561. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Uroporphyrinogen decarboxylase HAMAP-Rule MF_00218		PRO_0000187603
Regions
	Region	27 – 31	5	Substrate binding By similarity
Sites
	Binding site	46	1	Substrate By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	209	1	Substrate By similarity
	Binding site	327	1	Substrate By similarity
	Site	77	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	72 – 78	7	AILFSDI → RSSFRY in BAA02148. Ref.1
	Sequence conflict	83	1	D → I in BAA02148. Ref.1
	Sequence conflict	89 – 103	15	LYFEA…FTSPV → SSILKPEKVRVLPRQI in BAA02148. Ref.1
	Sequence conflict	251 – 256	6	GGGQWL → SATVA in BAA02148. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29680 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 604933C50080260C	FASTA	354	39,248
	10 20 30 40 50 60 MTELKNDRYL RALLRQPVDV TPVWMMRQAG RYLPEYKATR AQAGDFMSLC KNAELACEVT 70 80 90 100 110 120 LQPLRRYPLD AAILFSDILT VPDAMGLGLY FEAGEGPRFT SPVTCKADVD KLPIPDPEDE 130 140 150 160 170 180 LGYVMNAVRT IRRELKGEVP LIGFSGSPWT LATYMVEGGS SKAFTVIKKM MYADPQALHA 190 200 210 220 230 240 LLDKLAKSVT LYLNAQIKAG AQAVMIFDTW GGVLTGRDYQ QFSLYYMHKI VDGLLRENDG 250 260 270 280 290 300 RRVPVTLFTK GGGQWLEAMA ETGCDALGLD WTTDIADARR RVGNKVALQG NMDPSMLYAP 310 320 330 340 350 PARIEEEVAT ILAGFGHGEG HVFNLGHGIH QDVPPEHAGV FVEAVHRLSE QYHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the hemE gene encoding uroporphyrinogen III decarboxylase (UPD) from Escherichia coli K-12." Nishimura K., Inokuchi H. Gene 133:109-113(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12624 Genomic DNA. Translation: BAA02148.1. U00006 Genomic DNA. Translation: AAC43095.1. U00096 Genomic DNA. Translation: AAC76971.1. AP009048 Genomic DNA. Translation: BAE77322.1.
PIR	H65206.
RefSeq	NP_418425.1. NC_000913.2. YP_491463.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P29680.
SMR	P29680. Positions 3-354.
ModBase	Search...
Protein-protein interaction databases
IntAct	P29680. 5 interactions.
STRING	511145.b3997.
Proteomic databases
PaxDb	P29680.
PRIDE	P29680.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76971; AAC76971; b3997. BAE77322; BAE77322; BAE77322.
GeneID	12934224. 948497.
KEGG	ecj:Y75_p3199. eco:b3997.
PATRIC	32123517. VBIEscCol129921_4111.
Organism-specific databases
EchoBASE	EB1505.
EcoGene	EG11543. hemE.
Phylogenomic databases
eggNOG	COG0407.
HOGENOM	HOG000253896.
KO	K01599.
OMA	TLATYMI.
ProtClustDB	PRK00115.
Enzyme and pathway databases
BioCyc	EcoCyc:UROGENDECARBOX-MONOMER. ECOL316407:JW3961-MONOMER. MetaCyc:UROGENDECARBOX-MONOMER.
UniPathway	UPA00251; UER00321.
Gene expression databases
Genevestigator	P29680.
Family and domain databases
HAMAP	MF_00218. URO-D.
InterPro	IPR006361. Uroporphyrinogen_deCO2ase_HemE. IPR000257. Uroporphyrinogen_deCOase. [Graphical view]
PANTHER	PTHR21091:SF2. PTHR21091:SF2. 1 hit.
Pfam	PF01208. URO-D. 1 hit. [Graphical view]
TIGRFAMs	TIGR01464. hemE. 1 hit.
PROSITE	PS00906. UROD_1. 1 hit. PS00907. UROD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCUP_ECOLI

Accession

Primary (citable) accession number: P29680
Secondary accession number(s): P78135, Q2M8T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program