P29677 (MPPA_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial-processing peptidase subunit alpha
EC=3.4.24.64

Alternative name(s):
Alpha-MPP
Ubiquinol-cytochrome-c reductase subunit II
EC=1.10.2.2

Gene names

Name:

MPP

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves presequences (transit peptides) from mitochondrial protein precursors By similarity. This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. Mediate formation of the complex between cytochromes c and c1.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2. QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Subunit structure	Heterodimer of alpha and beta subunits.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the peptidase M16 family.
Caution	Does not seem to have a protease activity as it lack the zinc-binding site.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Molecular function	Hydrolase Metalloprotease Oxidoreductase Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 504		Mitochondrial-processing peptidase subunit alpha		PRO_0000026776

Sequences

	Sequence		Length	Mass (Da)
	P29677 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: FD3053688571D987	FASTA	504	54,677
	10 20 30 40 50 60 MYRCASSRLS SLKARQGNRV LTRFSSSAAV ATKPSGGLFS WITGDTSSSV TPLDFPLNDV 70 80 90 100 110 120 KLSPPLPDYV EPAKTQITTL ANGLKVASEA SVNPAASIGL YVDCGSIYET PASYGATHLL 130 140 150 160 170 180 ERMAFKSTLN RSHLRIVREI EAIGGNVTAS ASREHMIYTY DALKTYVPQM VEMLADCVRN 190 200 210 220 230 240 PAFLDWEVKE QLEKVKAEIS EYSKNPQHLL LEAVHSAGYA GPYGNSLMAT EATINRLNST 250 260 270 280 290 300 VLEEFVAENY TAPRMVLAAS GVEHEEFLKV AEPLLSDLPK VATIEEPKPV YVGGDYRCQA 310 320 330 340 350 360 DAEMTHFALA FEVPGGWMSE KESMTLTVLQ MLMGGGGSFS AGGPGKGMYS RLYLRVLNQY 370 380 390 400 410 420 PQIHAFSAFS SIYNNTGLFG IQGTTSSDFG PQAVDVAVKE LIAVANPSEV DQVQLNRAKQ 430 440 450 460 470 480 ATKSAILMNL ESRMVASEDI GRQLLTYGER NPVEHFLKAI DAVSAKDIAS VVQKLISSPL 490 500 TMASYGDVLS LPSYDAVSSR FRSK

References

[1]	"The general mitochondrial processing peptidase from potato is an integral part of cytochrome c reductase of the respiratory chain." Braun H.P., Emmermann M., Kruft V., Schmitz U.K. EMBO J. 11:3219-3227(1992) [PubMed: 1324169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 270-280 AND 400-419. Strain: cv. Desiree. Tissue: Green leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66284 mRNA. Translation: CAA46990.1.
PIR	S23558.
3D structure databases
ProteinModelPortal	P29677.
ModBase	Search...
Protein-protein interaction databases
IntAct	P29677. 1 interaction.
Protein family/group databases
MEROPS	M16.971.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011249. Metalloenz_metal-bd. IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
SUPFAM	SSF63411. Metalloenz_metal-bd. 2 hits.
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPPA_SOLTU

Accession

Primary (citable) accession number: P29677

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 16, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program