P29676 (EPO_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Erythropoietin

Gene names

Name:

Epo

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
Subcellular location	Secreted.
Tissue specificity	Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Sequence similarities	Belongs to the EPO/TPO family.

Ontologies

Keywords
Biological process	Erythrocyte maturation
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 19330822. Source: RGD apoptotic process Inferred from electronic annotation. Source: Compara cellular hyperosmotic response Inferred from electronic annotation. Source: Compara embryo implantation Inferred from electronic annotation. Source: Compara erythrocyte maturation Inferred from electronic annotation. Source: UniProtKB-KW hemoglobin biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of ion transmembrane transporter activity Inferred from electronic annotation. Source: Compara negative regulation of myeloid cell apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of sodium ion transport Inferred from electronic annotation. Source: Compara peptidyl-serine phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of DNA replication Inferred from electronic annotation. Source: Compara positive regulation of Ras protein signal transduction Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of neuron differentiation Inferred from direct assay PubMed 12876482. Source: RGD positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of tyrosine phosphorylation of Stat5 protein Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara response to axon injury Inferred from expression pattern PubMed 15470751. Source: RGD response to electrical stimulus Inferred from expression pattern PubMed 19185286. Source: RGD response to estrogen stimulus Inferred from expression pattern PubMed 15784734. Source: RGD response to hyperoxia Inferred from expression pattern PubMed 19768476. Source: RGD response to hypoxia Inferred from expression pattern PubMed 19497871. Source: RGD response to interleukin-1 Inferred from expression pattern PubMed 9321887. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 9321887. Source: RGD response to salt stress Inferred from expression pattern PubMed 16815869. Source: RGD response to testosterone stimulus Inferred from expression pattern PubMed 15778925. Source: RGD response to vitamin A Inferred from expression pattern PubMed 9027729. Source: RGD
Cellular_component	extracellular space Inferred from direct assay PubMed 19136608. Source: RGD
Molecular_function	eukaryotic cell surface binding Inferred from electronic annotation. Source: Compara protein kinase activator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	By similarity
	Chain	27 – 192	166	Erythropoietin		PRO_0000008408
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) By similarity
	Glycosylation	64	1	N-linked (GlcNAc...) By similarity
	Glycosylation	109	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	33 ↔ 187		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29676 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 3EA632737E7D2443	FASTA	192	21,286
	10 20 30 40 50 60 MGVPERPTLL LLLSLLLIPL GLPVLCAPPR LICDSRVLER YILEAKEAEN VTMGCAEGPR 70 80 90 100 110 120 LSENITVPDT KVNFYAWKRM KVEEQAVEVW QGLSLLSEAI LQAQALQANS SQPPESLQLH 130 140 150 160 170 180 IDKAISGLRS LTSLLRVLGA QKELMSPPDA TQAAPLRTLT ADTFCKLFRV YSNFLRGKLK 190 LYTGEACRRG DR

References

[1]	"Nucleotide sequence of rat erythropoietin." Nagao M., Suga H., Okano M., Masuda S., Narita H., Ikura K., Sasaki R. Biochim. Biophys. Acta 1171:99-102(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Kidney.
[2]	"Erythropoietin structure-function relationships: high degree of sequence homology among mammals." Wen D., Boissel J.-P.R., Tracy T.E., Gruninger R.H., Mulcahy L.S., Czelusniak J., Goodman M., Bunn H.F. Blood 82:1507-1516(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-192. Strain: Sprague-Dawley. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10763 mRNA. Translation: BAA01593.1. L10608 mRNA. Translation: AAA41126.1.
IPI	IPI00206819.
PIR	S28148.
RefSeq	NP_058697.1. NM_017001.1.
UniGene	Rn.11365.
3D structure databases
ProteinModelPortal	P29676.
SMR	P29676. Positions 27-192.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000001914.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000001914; ENSRNOP00000001914; ENSRNOG00000001412.
GeneID	24335.
KEGG	rno:24335.
UCSC	RGD:2559. rat.
Organism-specific databases
CTD	2056.
RGD	2559. Epo.
Phylogenomic databases
eggNOG	NOG43828.
GeneTree	ENSGT00390000017226.
HOGENOM	HOG000052505.
HOVERGEN	HBG003978.
InParanoid	P29676.
KO	K05437.
OrthoDB	EOG41VK4C.
Gene expression databases
Genevestigator	P29676.
GermOnline	ENSRNOG00000001412. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR019767. EPO/TPO_CS. IPR001323. EPO_TPO. IPR003013. Erythroptn. [Graphical view]
PANTHER	PTHR10370. PTHR10370. 1 hit.
Pfam	PF00758. EPO_TPO. 1 hit. [Graphical view]
PIRSF	PIRSF001951. EPO. 1 hit.
PRINTS	PR00272. ERYTHROPTN.
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00817. EPO_TPO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603023.

Entry information

Entry name

EPO_RAT

Accession

Primary (citable) accession number: P29676
Secondary accession number(s): P70504

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program