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Protein

Kallistatin

Gene

SERPINA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.1 Publication

Miscellaneous

Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei388 – 389Reactive bond2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-HSA-114608. Platelet degranulation.

Protein family/group databases

MEROPSiI04.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallistatin
Alternative name(s):
Kallikrein inhibitor
Peptidase inhibitor 4
Short name:
PI-4
Serpin A4
Gene namesi
Name:SERPINA4
Synonyms:KST, PI4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100665.11.
HGNCiHGNC:8948. SERPINA4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5267.
OpenTargetsiENSG00000100665.
PharmGKBiPA35514.

Polymorphism and mutation databases

DMDMi68067608.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003242521 – 427KallistatinAdd BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi108N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi157N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi238N-linked (GlcNAc...) (complex) asparagine2 Publications1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP29622.
PeptideAtlasiP29622.
PRIDEiP29622.
TopDownProteomicsiP29622.

PTM databases

iPTMnetiP29622.
PhosphoSitePlusiP29622.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiENSG00000100665.
ExpressionAtlasiP29622. baseline and differential.
GenevisibleiP29622. HS.

Organism-specific databases

HPAiHPA003607.

Interactioni

Subunit structurei

Monomer and some homodimers.

Protein-protein interaction databases

BioGridi111285. 15 interactors.
IntActiP29622. 10 interactors.
MINTiMINT-8247389.
STRINGi9606.ENSP00000298841.

Structurei

3D structure databases

ProteinModelPortaliP29622.
SMRiP29622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118839.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiP29622.
KOiK04525.
OMAiHWYLHDR.
OrthoDBiEOG091G0ION.
PhylomeDBiP29622.
TreeFamiTF343201.

Family and domain databases

InterProiView protein in InterPro
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiView protein in Pfam
PF00079. Serpin. 1 hit.
SMARTiView protein in SMART
SM00093. SERPIN. 1 hit.
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiView protein in PROSITE
PS00284. SERPIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLIDYLLLL LVGLLALSHG QLHVEHDGES CSNSSHQQIL ETGEGSPSLK
60 70 80 90 100
IAPANADFAF RFYYLIASET PGKNIFFSPL SISAAYAMLS LGACSHSRSQ
110 120 130 140 150
ILEGLGFNLT ELSESDVHRG FQHLLHTLNL PGHGLETRVG SALFLSHNLK
160 170 180 190 200
FLAKFLNDTM AVYEAKLFHT NFYDTVGTIQ LINDHVKKET RGKIVDLVSE
210 220 230 240 250
LKKDVLMVLV NYIYFKALWE KPFISSRTTP KDFYVDENTT VRVPMMLQDQ
260 270 280 290 300
EHHWYLHDRY LPCSVLRMDY KGDATVFFIL PNQGKMREIE EVLTPEMLMR
310 320 330 340 350
WNNLLRKRNF YKKLELHLPK FSISGSYVLD QILPRLGFTD LFSKWADLSG
360 370 380 390 400
ITKQQKLEAS KSFHKATLDV DEAGTEAAAA TSFAIKFFSA QTNRHILRFN
410 420
RPFLVVIFST STQSVLFLGK VVDPTKP
Length:427
Mass (Da):48,542
Last modified:June 21, 2005 - v3
Checksum:i68EBE7AF956BFB77
GO

Sequence cautioni

The sequence CAD66567 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti382S → T (PubMed:8227002).Curated1
Sequence conflicti382S → T (PubMed:7835886).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19684 mRNA. Translation: AAA59454.1.
L28101 Genomic DNA. Translation: AAC41706.1.
BX248009 mRNA. Translation: CAD62337.1.
BX248760 mRNA. Translation: CAD66567.1. Different initiation.
BC014992 mRNA. Translation: AAH14992.1.
CCDSiCCDS9927.1.
PIRiA49518.
RefSeqiNP_001275961.1. NM_001289032.1.
NP_001275962.1. NM_001289033.1.
NP_006206.2. NM_006215.3.
UniGeneiHs.719893.

Genome annotation databases

EnsembliENST00000298841; ENSP00000298841; ENSG00000100665.
ENST00000555095; ENSP00000451172; ENSG00000100665.
ENST00000557004; ENSP00000450838; ENSG00000100665.
GeneIDi5267.
KEGGihsa:5267.
UCSCiuc001ydk.5. human.

Similar proteinsi

Entry informationi

Entry nameiKAIN_HUMAN
AccessioniPrimary (citable) accession number: P29622
Secondary accession number(s): Q53XB5, Q86TR9, Q96BZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 21, 2005
Last modified: September 27, 2017
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families