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P29622

- KAIN_HUMAN

UniProt

P29622 - KAIN_HUMAN

Protein

Kallistatin

Gene

SERPINA4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 3 (21 Jun 2005)
      Previous versions | rss
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    Functioni

    Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei388 – 3892Reactive bond

    GO - Molecular functioni

    1. serine-type endopeptidase inhibitor activity Source: RefGenome

    GO - Biological processi

    1. negative regulation of endopeptidase activity Source: RefGenome
    2. regulation of proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI04.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kallistatin
    Alternative name(s):
    Kallikrein inhibitor
    Peptidase inhibitor 4
    Short name:
    PI-4
    Serpin A4
    Gene namesi
    Name:SERPINA4
    Synonyms:KST, PI4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:8948. SERPINA4.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: BHF-UCL
    2. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35514.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 427407KallistatinPRO_0000032425Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi108 – 1081N-linked (GlcNAc...)1 Publication
    Glycosylationi157 – 1571N-linked (GlcNAc...)2 Publications
    Glycosylationi238 – 2381N-linked (GlcNAc...) (complex)2 Publications

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP29622.
    PeptideAtlasiP29622.
    PRIDEiP29622.

    PTM databases

    PhosphoSiteiP29622.

    Expressioni

    Tissue specificityi

    Expressed by the liver and secreted in plasma.

    Gene expression databases

    BgeeiP29622.
    GenevestigatoriP29622.

    Organism-specific databases

    HPAiHPA003607.

    Interactioni

    Subunit structurei

    Monomer and some homodimers.

    Protein-protein interaction databases

    BioGridi111285. 12 interactions.
    IntActiP29622. 10 interactions.
    MINTiMINT-8247389.
    STRINGi9606.ENSP00000298841.

    Structurei

    3D structure databases

    ProteinModelPortaliP29622.
    SMRiP29622. Positions 57-424.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the serpin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4826.
    HOGENOMiHOG000238521.
    HOVERGENiHBG005957.
    InParanoidiP29622.
    OMAiVPMMLQD.
    OrthoDBiEOG7QC7W9.
    PhylomeDBiP29622.
    TreeFamiTF343201.

    Family and domain databases

    InterProiIPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view]
    PANTHERiPTHR11461. PTHR11461. 1 hit.
    PfamiPF00079. Serpin. 1 hit.
    [Graphical view]
    SMARTiSM00093. SERPIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF56574. SSF56574. 1 hit.
    PROSITEiPS00284. SERPIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29622-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHLIDYLLLL LVGLLALSHG QLHVEHDGES CSNSSHQQIL ETGEGSPSLK    50
    IAPANADFAF RFYYLIASET PGKNIFFSPL SISAAYAMLS LGACSHSRSQ 100
    ILEGLGFNLT ELSESDVHRG FQHLLHTLNL PGHGLETRVG SALFLSHNLK 150
    FLAKFLNDTM AVYEAKLFHT NFYDTVGTIQ LINDHVKKET RGKIVDLVSE 200
    LKKDVLMVLV NYIYFKALWE KPFISSRTTP KDFYVDENTT VRVPMMLQDQ 250
    EHHWYLHDRY LPCSVLRMDY KGDATVFFIL PNQGKMREIE EVLTPEMLMR 300
    WNNLLRKRNF YKKLELHLPK FSISGSYVLD QILPRLGFTD LFSKWADLSG 350
    ITKQQKLEAS KSFHKATLDV DEAGTEAAAA TSFAIKFFSA QTNRHILRFN 400
    RPFLVVIFST STQSVLFLGK VVDPTKP 427
    Length:427
    Mass (Da):48,542
    Last modified:June 21, 2005 - v3
    Checksum:i68EBE7AF956BFB77
    GO

    Sequence cautioni

    The sequence CAD66567.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti382 – 3821S → T(PubMed:8227002)Curated
    Sequence conflicti382 – 3821S → T(PubMed:7835886)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19684 mRNA. Translation: AAA59454.1.
    L28101 Genomic DNA. Translation: AAC41706.1.
    BX248009 mRNA. Translation: CAD62337.1.
    BX248760 mRNA. Translation: CAD66567.1. Different initiation.
    BC014992 mRNA. Translation: AAH14992.1.
    CCDSiCCDS9927.1.
    PIRiA49518.
    RefSeqiNP_001275961.1. NM_001289032.1.
    NP_001275962.1. NM_001289033.1.
    NP_006206.2. NM_006215.3.
    UniGeneiHs.719893.

    Genome annotation databases

    EnsembliENST00000298841; ENSP00000298841; ENSG00000100665.
    ENST00000555095; ENSP00000451172; ENSG00000100665.
    ENST00000557004; ENSP00000450838; ENSG00000100665.
    GeneIDi5267.
    KEGGihsa:5267.
    UCSCiuc001ydk.3. human.

    Polymorphism databases

    DMDMi68067608.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19684 mRNA. Translation: AAA59454.1 .
    L28101 Genomic DNA. Translation: AAC41706.1 .
    BX248009 mRNA. Translation: CAD62337.1 .
    BX248760 mRNA. Translation: CAD66567.1 . Different initiation.
    BC014992 mRNA. Translation: AAH14992.1 .
    CCDSi CCDS9927.1.
    PIRi A49518.
    RefSeqi NP_001275961.1. NM_001289032.1.
    NP_001275962.1. NM_001289033.1.
    NP_006206.2. NM_006215.3.
    UniGenei Hs.719893.

    3D structure databases

    ProteinModelPortali P29622.
    SMRi P29622. Positions 57-424.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111285. 12 interactions.
    IntActi P29622. 10 interactions.
    MINTi MINT-8247389.
    STRINGi 9606.ENSP00000298841.

    Protein family/group databases

    MEROPSi I04.003.

    PTM databases

    PhosphoSitei P29622.

    Polymorphism databases

    DMDMi 68067608.

    Proteomic databases

    PaxDbi P29622.
    PeptideAtlasi P29622.
    PRIDEi P29622.

    Protocols and materials databases

    DNASUi 5267.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000298841 ; ENSP00000298841 ; ENSG00000100665 .
    ENST00000555095 ; ENSP00000451172 ; ENSG00000100665 .
    ENST00000557004 ; ENSP00000450838 ; ENSG00000100665 .
    GeneIDi 5267.
    KEGGi hsa:5267.
    UCSCi uc001ydk.3. human.

    Organism-specific databases

    CTDi 5267.
    GeneCardsi GC14P095027.
    HGNCi HGNC:8948. SERPINA4.
    HPAi HPA003607.
    MIMi 147935. gene.
    neXtProti NX_P29622.
    PharmGKBi PA35514.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG4826.
    HOGENOMi HOG000238521.
    HOVERGENi HBG005957.
    InParanoidi P29622.
    OMAi VPMMLQD.
    OrthoDBi EOG7QC7W9.
    PhylomeDBi P29622.
    TreeFami TF343201.

    Miscellaneous databases

    GeneWikii SERPINA4.
    GenomeRNAii 5267.
    NextBioi 20348.
    PROi P29622.
    SOURCEi Search...

    Gene expression databases

    Bgeei P29622.
    Genevestigatori P29622.

    Family and domain databases

    InterProi IPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view ]
    PANTHERi PTHR11461. PTHR11461. 1 hit.
    Pfami PF00079. Serpin. 1 hit.
    [Graphical view ]
    SMARTi SM00093. SERPIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56574. SSF56574. 1 hit.
    PROSITEi PS00284. SERPIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Kallistatin: a novel human serine proteinase inhibitor. Molecular cloning, tissue distribution, and expression in Escherichia coli."
      Chai K.X., Chen L.-M., Chao J., Chao L.
      J. Biol. Chem. 268:24498-24505(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "Molecular cloning, sequence analysis, and chromosomal localization of the human protease inhibitor 4 (kallistatin) gene (PI4)."
      Chai K.X., Ward D.C., Chao J., Chao L.
      Genomics 23:370-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Full-length cDNA libraries and normalization."
      Li W.B., Gruber C., Jessee J., Polayes D.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Fetal liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    5. "Kallistatin: a novel human tissue kallikrein inhibitor. Purification, characterization, and reactive center sequence."
      Zhou G.X., Chao L., Chao J.
      J. Biol. Chem. 267:25873-25880(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 388-403.
      Tissue: Plasma.
    6. "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
      Zhang H., Li X.-J., Martin D.B., Aebersold R.
      Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT ASN-157.
    7. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
      J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-108; ASN-157 AND ASN-238.
      Tissue: Plasma.
    8. Cited for: GLYCOSYLATION AT ASN-238.

    Entry informationi

    Entry nameiKAIN_HUMAN
    AccessioniPrimary (citable) accession number: P29622
    Secondary accession number(s): Q53XB5, Q86TR9, Q96BZ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 131 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3