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P29622

- KAIN_HUMAN

UniProt

P29622 - KAIN_HUMAN

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Protein

Kallistatin

Gene

SERPINA4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei388 – 3892Reactive bond

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: RefGenome

GO - Biological processi

  1. negative regulation of endopeptidase activity Source: RefGenome
  2. regulation of proteolysis Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallistatin
Alternative name(s):
Kallikrein inhibitor
Peptidase inhibitor 4
Short name:
PI-4
Serpin A4
Gene namesi
Name:SERPINA4
Synonyms:KST, PI4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 14

Organism-specific databases

HGNCiHGNC:8948. SERPINA4.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: BHF-UCL
  2. extracellular vesicular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35514.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 427407KallistatinPRO_0000032425Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi108 – 1081N-linked (GlcNAc...)1 Publication
Glycosylationi157 – 1571N-linked (GlcNAc...)2 Publications
Glycosylationi238 – 2381N-linked (GlcNAc...) (complex)2 Publications

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP29622.
PeptideAtlasiP29622.
PRIDEiP29622.

PTM databases

PhosphoSiteiP29622.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiP29622.
GenevestigatoriP29622.

Organism-specific databases

HPAiHPA003607.

Interactioni

Subunit structurei

Monomer and some homodimers.

Protein-protein interaction databases

BioGridi111285. 15 interactions.
IntActiP29622. 10 interactions.
MINTiMINT-8247389.
STRINGi9606.ENSP00000298841.

Structurei

3D structure databases

ProteinModelPortaliP29622.
SMRiP29622. Positions 57-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4826.
GeneTreeiENSGT00760000118839.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiP29622.
OMAiVPMMLQD.
OrthoDBiEOG7QC7W9.
PhylomeDBiP29622.
TreeFamiTF343201.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29622-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHLIDYLLLL LVGLLALSHG QLHVEHDGES CSNSSHQQIL ETGEGSPSLK
60 70 80 90 100
IAPANADFAF RFYYLIASET PGKNIFFSPL SISAAYAMLS LGACSHSRSQ
110 120 130 140 150
ILEGLGFNLT ELSESDVHRG FQHLLHTLNL PGHGLETRVG SALFLSHNLK
160 170 180 190 200
FLAKFLNDTM AVYEAKLFHT NFYDTVGTIQ LINDHVKKET RGKIVDLVSE
210 220 230 240 250
LKKDVLMVLV NYIYFKALWE KPFISSRTTP KDFYVDENTT VRVPMMLQDQ
260 270 280 290 300
EHHWYLHDRY LPCSVLRMDY KGDATVFFIL PNQGKMREIE EVLTPEMLMR
310 320 330 340 350
WNNLLRKRNF YKKLELHLPK FSISGSYVLD QILPRLGFTD LFSKWADLSG
360 370 380 390 400
ITKQQKLEAS KSFHKATLDV DEAGTEAAAA TSFAIKFFSA QTNRHILRFN
410 420
RPFLVVIFST STQSVLFLGK VVDPTKP
Length:427
Mass (Da):48,542
Last modified:June 21, 2005 - v3
Checksum:i68EBE7AF956BFB77
GO

Sequence cautioni

The sequence CAD66567.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti382 – 3821S → T(PubMed:8227002)Curated
Sequence conflicti382 – 3821S → T(PubMed:7835886)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19684 mRNA. Translation: AAA59454.1.
L28101 Genomic DNA. Translation: AAC41706.1.
BX248009 mRNA. Translation: CAD62337.1.
BX248760 mRNA. Translation: CAD66567.1. Different initiation.
BC014992 mRNA. Translation: AAH14992.1.
CCDSiCCDS9927.1.
PIRiA49518.
RefSeqiNP_001275961.1. NM_001289032.1.
NP_001275962.1. NM_001289033.1.
NP_006206.2. NM_006215.3.
UniGeneiHs.719893.

Genome annotation databases

EnsembliENST00000298841; ENSP00000298841; ENSG00000100665.
ENST00000555095; ENSP00000451172; ENSG00000100665.
ENST00000557004; ENSP00000450838; ENSG00000100665.
GeneIDi5267.
KEGGihsa:5267.
UCSCiuc001ydk.3. human.

Polymorphism databases

DMDMi68067608.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19684 mRNA. Translation: AAA59454.1 .
L28101 Genomic DNA. Translation: AAC41706.1 .
BX248009 mRNA. Translation: CAD62337.1 .
BX248760 mRNA. Translation: CAD66567.1 . Different initiation.
BC014992 mRNA. Translation: AAH14992.1 .
CCDSi CCDS9927.1.
PIRi A49518.
RefSeqi NP_001275961.1. NM_001289032.1.
NP_001275962.1. NM_001289033.1.
NP_006206.2. NM_006215.3.
UniGenei Hs.719893.

3D structure databases

ProteinModelPortali P29622.
SMRi P29622. Positions 57-424.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111285. 15 interactions.
IntActi P29622. 10 interactions.
MINTi MINT-8247389.
STRINGi 9606.ENSP00000298841.

Protein family/group databases

MEROPSi I04.003.

PTM databases

PhosphoSitei P29622.

Polymorphism databases

DMDMi 68067608.

Proteomic databases

PaxDbi P29622.
PeptideAtlasi P29622.
PRIDEi P29622.

Protocols and materials databases

DNASUi 5267.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000298841 ; ENSP00000298841 ; ENSG00000100665 .
ENST00000555095 ; ENSP00000451172 ; ENSG00000100665 .
ENST00000557004 ; ENSP00000450838 ; ENSG00000100665 .
GeneIDi 5267.
KEGGi hsa:5267.
UCSCi uc001ydk.3. human.

Organism-specific databases

CTDi 5267.
GeneCardsi GC14P095027.
HGNCi HGNC:8948. SERPINA4.
HPAi HPA003607.
MIMi 147935. gene.
neXtProti NX_P29622.
PharmGKBi PA35514.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG4826.
GeneTreei ENSGT00760000118839.
HOGENOMi HOG000238521.
HOVERGENi HBG005957.
InParanoidi P29622.
OMAi VPMMLQD.
OrthoDBi EOG7QC7W9.
PhylomeDBi P29622.
TreeFami TF343201.

Miscellaneous databases

GeneWikii SERPINA4.
GenomeRNAii 5267.
NextBioi 20348.
PROi P29622.
SOURCEi Search...

Gene expression databases

Bgeei P29622.
Genevestigatori P29622.

Family and domain databases

InterProi IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view ]
PANTHERi PTHR11461. PTHR11461. 1 hit.
Pfami PF00079. Serpin. 1 hit.
[Graphical view ]
SMARTi SM00093. SERPIN. 1 hit.
[Graphical view ]
SUPFAMi SSF56574. SSF56574. 1 hit.
PROSITEi PS00284. SERPIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Kallistatin: a novel human serine proteinase inhibitor. Molecular cloning, tissue distribution, and expression in Escherichia coli."
    Chai K.X., Chen L.-M., Chao J., Chao L.
    J. Biol. Chem. 268:24498-24505(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "Molecular cloning, sequence analysis, and chromosomal localization of the human protease inhibitor 4 (kallistatin) gene (PI4)."
    Chai K.X., Ward D.C., Chao J., Chao L.
    Genomics 23:370-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Full-length cDNA libraries and normalization."
    Li W.B., Gruber C., Jessee J., Polayes D.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  5. "Kallistatin: a novel human tissue kallikrein inhibitor. Purification, characterization, and reactive center sequence."
    Zhou G.X., Chao L., Chao J.
    J. Biol. Chem. 267:25873-25880(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 388-403.
    Tissue: Plasma.
  6. "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."
    Zhang H., Li X.-J., Martin D.B., Aebersold R.
    Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-157.
  7. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
    Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
    J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-108; ASN-157 AND ASN-238.
    Tissue: Plasma.
  8. Cited for: GLYCOSYLATION AT ASN-238.

Entry informationi

Entry nameiKAIN_HUMAN
AccessioniPrimary (citable) accession number: P29622
Secondary accession number(s): Q53XB5, Q86TR9, Q96BZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 21, 2005
Last modified: October 29, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3