ID CDKA2_ORYSJ Reviewed; 292 AA. AC P29619; B7ERM3; Q0E4E3; Q6Z721; Q6Z722; DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot. DT 01-APR-1993, sequence version 1. DT 24-JAN-2024, entry version 155. DE RecName: Full=Cyclin-dependent kinase A-2; DE Short=CDKA;2; DE EC=2.7.11.22; DE EC=2.7.11.23; DE AltName: Full=CDC2Os-2; DE AltName: Full=Cell division control protein 2 homolog 2; GN Name=CDKA-2; Synonyms=CDC2-2; GN OrderedLocusNames=Os02g0123100, LOC_Os02g03060; GN ORFNames=P0575F10.10-1, P0575F10.10-2; OS Oryza sativa subsp. japonica (Rice). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa. OX NCBI_TaxID=39947; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Nipponbare; RX PubMed=1376401; DOI=10.1007/bf00587555; RA Hashimoto J., Hirabayashi T., Hayano Y., Hata S., Ohashi Y., Suzuka I., RA Utsugi T., Toh-e A., Kikuchi Y.; RT "Isolation and characterization of cDNA clones encoding cdc2 homologues RT from Oryza sativa: a functional homologue and cognate variants."; RL Mol. Gen. Genet. 233:10-16(1992). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Nipponbare; RX PubMed=16100779; DOI=10.1038/nature03895; RG International rice genome sequencing project (IRGSP); RT "The map-based sequence of the rice genome."; RL Nature 436:793-800(2005). RN [3] RP GENOME REANNOTATION. RC STRAIN=cv. Nipponbare; RX PubMed=18089549; DOI=10.1093/nar/gkm978; RG The rice annotation project (RAP); RT "The rice annotation project database (RAP-DB): 2008 update."; RL Nucleic Acids Res. 36:D1028-D1033(2008). RN [4] RP GENOME REANNOTATION. RC STRAIN=cv. Nipponbare; RX PubMed=24280374; DOI=10.1186/1939-8433-6-4; RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R., RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L., RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H., RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.; RT "Improvement of the Oryza sativa Nipponbare reference genome using next RT generation sequence and optical map data."; RL Rice 6:4-4(2013). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Nipponbare; RX PubMed=12869764; DOI=10.1126/science.1081288; RG The rice full-length cDNA consortium; RT "Collection, mapping, and annotation of over 28,000 cDNA clones from RT japonica rice."; RL Science 301:376-379(2003). RN [6] RP TISSUE SPECIFICITY, AND INDUCTION. RX PubMed=7742859; DOI=10.1046/j.1365-313x.1995.7040623.x; RA Sauter M., Mekhedov S.L., Kende H.; RT "Gibberellin promotes histone H1 kinase activity and the expression of cdc2 RT and cyclin genes during the induction of rapid growth in deepwater rice RT internodes."; RL Plant J. 7:623-632(1995). RN [7] RP DEVELOPMENTAL STAGE. RX PubMed=9076986; DOI=10.1046/j.1365-313x.1997.11020181.x; RA Sauter M.; RT "Differential expression of a CAK (cdc2-activating kinase)-like protein RT kinase, cyclins and cdc2 genes from rice during the cell cycle and in RT response to gibberellin."; RL Plant J. 11:181-190(1997). RN [8] RP INDUCTION. RX PubMed=9880342; DOI=10.1104/pp.119.1.21; RA Lorbiecke R., Sauter M.; RT "Adventitious root growth and cell-cycle induction in deepwater rice."; RL Plant Physiol. 119:21-30(1999). RN [9] RP TISSUE SPECIFICITY. RX PubMed=9880343; DOI=10.1104/pp.119.1.31; RA Umeda M., Umeda-Hara C., Yamaguchi M., Hashimoto J., Uchimiya H.; RT "Differential expression of genes for cyclin-dependent protein kinases in RT rice plants."; RL Plant Physiol. 119:31-40(1999). RN [10] RP INDUCTION AND GENE FAMILY. RX PubMed=17443292; DOI=10.1007/s11103-007-9154-y; RA Guo J., Song J., Wang F., Zhang X.S.; RT "Genome-wide identification and expression analysis of rice cell cycle RT genes."; RL Plant Mol. Biol. 64:349-360(2007). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl- CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA- CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.22; CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L- CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; CC EC=2.7.11.22; CC -!- CATALYTIC ACTIVITY: CC Reaction=[DNA-directed RNA polymerase] + ATP = ADP + H(+) + phospho- CC [DNA-directed RNA polymerase]; Xref=Rhea:RHEA:10216, Rhea:RHEA- CC COMP:11321, Rhea:RHEA-COMP:11322, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43176, ChEBI:CHEBI:68546, CC ChEBI:CHEBI:456216; EC=2.7.11.23; CC -!- TISSUE SPECIFICITY: Expressed in the dividing region of the root apex CC and in differentiated cells such as those in the sclerenchyma, CC pericycle and parenchyma of the central cylinder. Expressed in the CC intercalary meristem and the elongation zone of internodes. CC {ECO:0000269|PubMed:7742859, ECO:0000269|PubMed:9880343}. CC -!- DEVELOPMENTAL STAGE: Expression reaches a peak in the G1/S phases and CC then decreases in the G2/M phases. {ECO:0000269|PubMed:9076986}. CC -!- INDUCTION: By gibberellic acid (GA3) and submergence. Down-regulated by CC auxin. {ECO:0000269|PubMed:17443292, ECO:0000269|PubMed:7742859, CC ECO:0000269|PubMed:9880342}. CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr CC protein kinase family. CDC2/CDKX subfamily. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=BAD07950.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X60375; CAA42923.1; -; mRNA. DR EMBL; AP004885; BAD07949.1; -; Genomic_DNA. DR EMBL; AP004885; BAD07950.1; ALT_SEQ; Genomic_DNA. DR EMBL; AP008208; BAF07645.1; -; Genomic_DNA. DR EMBL; AP014958; BAS76730.1; -; Genomic_DNA. DR EMBL; AK101344; BAG95020.1; -; mRNA. DR PIR; S22441; S22441. DR RefSeq; XP_015623588.1; XM_015768102.1. DR AlphaFoldDB; P29619; -. DR SMR; P29619; -. DR STRING; 39947.P29619; -. DR PaxDb; 39947-P29619; -. DR EnsemblPlants; Os02t0123100-03; Os02t0123100-03; Os02g0123100. DR GeneID; 4328135; -. DR Gramene; Os02t0123100-03; Os02t0123100-03; Os02g0123100. DR KEGG; osa:4328135; -. DR eggNOG; KOG0594; Eukaryota. DR HOGENOM; CLU_000288_181_6_1; -. DR InParanoid; P29619; -. DR OrthoDB; 244018at2759; -. DR PlantReactome; R-OSA-9640760; G1 phase. DR PlantReactome; R-OSA-9640887; G1/S transition. DR Proteomes; UP000000763; Chromosome 2. DR Proteomes; UP000059680; Chromosome 2. DR ExpressionAtlas; P29619; baseline and differential. DR GO; GO:0000307; C:cyclin-dependent protein kinase holoenzyme complex; IBA:GO_Central. DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. DR GO; GO:0005634; C:nucleus; ISS:UniProtKB. DR GO; GO:0016581; C:NuRD complex; ISS:UniProtKB. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0030332; F:cyclin binding; IBA:GO_Central. DR GO; GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IBA:GO_Central. DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA. DR GO; GO:0008353; F:RNA polymerase II CTD heptapeptide repeat kinase activity; IEA:UniProtKB-EC. DR GO; GO:0000082; P:G1/S transition of mitotic cell cycle; IBA:GO_Central. DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW. DR GO; GO:0010389; P:regulation of G2/M transition of mitotic cell cycle; IBA:GO_Central. DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central. DR GO; GO:0051445; P:regulation of meiotic cell cycle; IBA:GO_Central. DR GO; GO:0010033; P:response to organic substance; IBA:GO_Central. DR GO; GO:0007165; P:signal transduction; IBA:GO_Central. DR CDD; cd07835; STKc_CDK1_CdkB_like; 1. DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1. DR InterPro; IPR011009; Kinase-like_dom_sf. DR InterPro; IPR000719; Prot_kinase_dom. DR InterPro; IPR017441; Protein_kinase_ATP_BS. DR InterPro; IPR008271; Ser/Thr_kinase_AS. DR PANTHER; PTHR24056; CELL DIVISION PROTEIN KINASE; 1. DR PANTHER; PTHR24056:SF548; CYCLIN-DEPENDENT KINASE A-1; 1. DR Pfam; PF00069; Pkinase; 1. DR SMART; SM00220; S_TKc; 1. DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1. DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1. DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1. DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1. DR Genevisible; P29619; OS. PE 2: Evidence at transcript level; KW ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein; KW Reference proteome; Serine/threonine-protein kinase; Transferase. FT CHAIN 1..292 FT /note="Cyclin-dependent kinase A-2" FT /id="PRO_0000085756" FT DOMAIN 4..286 FT /note="Protein kinase" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159" FT ACT_SITE 126 FT /note="Proton acceptor" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159, FT ECO:0000255|PROSITE-ProRule:PRU10027" FT BINDING 10..18 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159" FT BINDING 33 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159" FT MOD_RES 14 FT /note="Phosphothreonine" FT /evidence="ECO:0000250" FT MOD_RES 15 FT /note="Phosphotyrosine" FT /evidence="ECO:0000250" FT MOD_RES 160 FT /note="Phosphothreonine" FT /evidence="ECO:0000250" SQ SEQUENCE 292 AA; 33693 MW; C7791576349DFE22 CRC64; MEQYEKVEKI GEGTYGVVYK GKHRHTNETI ALKKIRLEQE DEGVPSTAIR EISLLKEMQH RNIVRLQDVV HKEKCIYLVF EYLDLDLKKH MDSSPDFKNH RIVKSFLYQI LRGIAYCHSH RVLHRDLKPQ NLLIDRRTNS LKLADFGLAR AFGIPVRTFT HEVVTLWYRA PEILLGARHY STPVDMWSVG CIFAEMVNQK PLFPGDSEID ELFKIFSIMG TPNEETWPGV ASLPDYISTF PKWPSVDLAT VVPTLDSSGL DLLSKMLRLD PSKRINARAA LEHEYFKDLE VA //