P29619 (CDKA2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase A-2
Short name=CDKA;2
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
CDC2Os-2
Cell division control protein 2 homolog 2

Gene names

Name:	CDKA-2
Synonyms:	CDC2-2
Ordered Locus Names:	Os02g0123100, LOC_Os02g03060
ORF Names:	P0575F10.10-1, P0575F10.10-2

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Tissue specificity	Expressed in the dividing region of the root apex and in differentiated cells such as those in the sclerenchyma, pericycle and parenchyma of the central cylinder. Expressed in the intercalary meristem and the elongation zone of internodes. Ref.4 Ref.7
Developmental stage	Expression reaches a peak in the G1/S phases and then decreases in the G2/M phases. Ref.5
Induction	By gibberellic acid (GA3) and submergence. Down-regulated by auxin. Ref.4 Ref.6 Ref.8
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence BAD07950.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Cyclin-dependent kinase A-2		PRO_0000085756
Regions
	Domain	4 – 286	283	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	126	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	160	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29619 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: C7791576349DFE22	FASTA	292	33,693
	10 20 30 40 50 60 MEQYEKVEKI GEGTYGVVYK GKHRHTNETI ALKKIRLEQE DEGVPSTAIR EISLLKEMQH 70 80 90 100 110 120 RNIVRLQDVV HKEKCIYLVF EYLDLDLKKH MDSSPDFKNH RIVKSFLYQI LRGIAYCHSH 130 140 150 160 170 180 RVLHRDLKPQ NLLIDRRTNS LKLADFGLAR AFGIPVRTFT HEVVTLWYRA PEILLGARHY 190 200 210 220 230 240 STPVDMWSVG CIFAEMVNQK PLFPGDSEID ELFKIFSIMG TPNEETWPGV ASLPDYISTF 250 260 270 280 290 PKWPSVDLAT VVPTLDSSGL DLLSKMLRLD PSKRINARAA LEHEYFKDLE VA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cDNA clones encoding cdc2 homologues from Oryza sativa: a functional homologue and cognate variants." Hashimoto J., Hirabayashi T., Hayano Y., Hata S., Ohashi Y., Suzuka I., Utsugi T., Toh-e A., Kikuchi Y. Mol. Gen. Genet. 233:10-16(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Gibberellin promotes histone H1 kinase activity and the expression of cdc2 and cyclin genes during the induction of rapid growth in deepwater rice internodes." Sauter M., Mekhedov S.L., Kende H. Plant J. 7:623-632(1995) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[5]	"Differential expression of a CAK (cdc2-activating kinase)-like protein kinase, cyclins and cdc2 genes from rice during the cell cycle and in response to gibberellin." Sauter M. Plant J. 11:181-190(1997) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[6]	"Adventitious root growth and cell-cycle induction in deepwater rice." Lorbiecke R., Sauter M. Plant Physiol. 119:21-30(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"Differential expression of genes for cyclin-dependent protein kinases in rice plants." Umeda M., Umeda-Hara C., Yamaguchi M., Hashimoto J., Uchimiya H. Plant Physiol. 119:31-40(1999) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Genome-wide identification and expression analysis of rice cell cycle genes." Guo J., Song J., Wang F., Zhang X.S. Plant Mol. Biol. 64:349-360(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION AND GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60375 mRNA. Translation: CAA42923.1. AP004885 Genomic DNA. Translation: BAD07949.1. AP004885 Genomic DNA. Translation: BAD07950.1. Sequence problems. AP008208 Genomic DNA. Translation: BAF07645.1.
PIR	S22441.
RefSeq	NP_001045731.1. NM_001052266.1.
UniGene	Os.3428.
3D structure databases
ProteinModelPortal	P29619.
SMR	P29619. Positions 1-288.
ModBase	Search...
Proteomic databases
PRIDE	P29619.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g03060.3; LOC_Os02g03060.3; LOC_Os02g03060.
GeneID	4328135.
KEGG	osa:4328135.
Organism-specific databases
Gramene	P29619.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K02206.
ProtClustDB	PLN00009.
Gene expression databases
ArrayExpress	P29619.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDKA2_ORYSJ

Accession

Primary (citable) accession number: P29619
Secondary accession number(s): Q0E4E3, Q6Z721, Q6Z722

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program