P29618 (CDKA1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase A-1
Short name=CDKA;1
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
CDC2Os-1
Cell division control protein 2 homolog 1

Gene names

Name:	CDKA-1
Synonyms:	CDC2-1
Ordered Locus Names:	Os03g0118400, LOC_Os03g02680

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Tissue specificity	Expressed in the dividing region of the root apex and in differentiated cells such as those in the sclerenchyma, pericycle and parenchyma of the central cylinder. Ref.7
Developmental stage	Expressed throughout the cell cycle. Ref.5
Induction	By submergence. Down-regulated by cytokinin. Ref.6 Ref.9
Post-translational modification	Phosphorylated at Thr-161 by CDKD-1. Ref.8
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Cyclin-dependent kinase A-1		PRO_0000085755
Regions
	Domain	4 – 287	284	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	161	1	Phosphothreonine; by CAK
Experimental info
	Mutagenesis	161	1	T → A: Loss of phosphorylation by CDKD-1. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P29618 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 51322D93AEF4C131	FASTA	294	34,071
	10 20 30 40 50 60 MEQYEKEEKI GEGTYGVVYR ARDKVTNETI ALKKIRLEQE DEGVPSTAIR EISLLKEMHH 70 80 90 100 110 120 GNIVRLHDVI HSEKRIYLVF EYLDLDLKKF MDSCPEFAKN PTLIKSYLYQ ILRGVAYCHS 130 140 150 160 170 180 HRVLHRDLKP QNLLIDRRTN ALKLADFGLA RAFGIPVRTF THEVVTLWYR APEILLGSRQ 190 200 210 220 230 240 YSTPVDMWSV GCIFAEMVNQ KPLFPGDSEI DELFKIFRVL GTPNEQSWPG VSSLPDYKSA 250 260 270 280 290 FPKWQAQDLA TIVPTLDPAG LDLLSKMLRY EPNKRITARQ ALEHEYFKDL EMVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cDNA clones encoding cdc2 homologues from Oryza sativa: a functional homologue and cognate variants." Hashimoto J., Hirabayashi T., Hayano Y., Hata S., Ohashi Y., Suzuka I., Utsugi T., Toh-e A., Kikuchi Y. Mol. Gen. Genet. 233:10-16(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Differential expression of a CAK (cdc2-activating kinase)-like protein kinase, cyclins and cdc2 genes from rice during the cell cycle and in response to gibberellin." Sauter M. Plant J. 11:181-190(1997) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[6]	"Adventitious root growth and cell-cycle induction in deepwater rice." Lorbiecke R., Sauter M. Plant Physiol. 119:21-30(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"Differential expression of genes for cyclin-dependent protein kinases in rice plants." Umeda M., Umeda-Hara C., Yamaguchi M., Hashimoto J., Uchimiya H. Plant Physiol. 119:31-40(1999) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Activation of CDK-activating kinase is dependent on interaction with H-type cyclins in plants." Yamaguchi M., Fabian T., Sauter M., Bhalerao R.P., Schrader J., Sandberg G., Umeda M., Uchimiya H. Plant J. 24:11-20(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, MUTAGENESIS OF THR-161.
[9]	"Genome-wide identification and expression analysis of rice cell cycle genes." Guo J., Song J., Wang F., Zhang X.S. Plant Mol. Biol. 64:349-360(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION AND GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60374 mRNA. Translation: CAA42922.1. DP000009 Genomic DNA. Translation: ABF93669.1. AP008209 Genomic DNA. Translation: BAF10686.1.
PIR	S22440.
RefSeq	NP_001048772.1. NM_001055307.1.
UniGene	Os.11723.
3D structure databases
ProteinModelPortal	P29618.
SMR	P29618. Positions 1-289.
ModBase	Search...
Proteomic databases
PRIDE	P29618.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g02680.2; LOC_Os03g02680.2; LOC_Os03g02680.
GeneID	4331415.
KEGG	dosa:Os03t0108800-01. dosa:Os03t0118400-01. osa:4331415.
Organism-specific databases
Gramene	P29618.
Phylogenomic databases
eggNOG	COG0515.
KO	K02206.
ProtClustDB	PLN00009.
Gene expression databases
ArrayExpress	P29618.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDKA1_ORYSJ

Accession

Primary (citable) accession number: P29618
Secondary accession number(s): Q10SL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program