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P29618 (CDKA1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase A-1

Short name=CDKA;1
EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
CDC2Os-1
Cell division control protein 2 homolog 1
Gene names
Name:CDKA-1
Synonyms:CDC2-1
Ordered Locus Names:Os03g0118400, LOC_Os03g02680
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Tissue specificity

Expressed in the dividing region of the root apex and in differentiated cells such as those in the sclerenchyma, pericycle and parenchyma of the central cylinder. Ref.7

Developmental stage

Expressed throughout the cell cycle. Ref.5

Induction

By submergence. Down-regulated by cytokinin. Ref.6 Ref.9

Post-translational modification

Phosphorylated at Thr-161 by CDKD-1. Ref.8

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294Cyclin-dependent kinase A-1
PRO_0000085755

Regions

Domain4 – 287284Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1271Proton acceptor By similarity
Binding site331ATP By similarity

Amino acid modifications

Modified residue141Phosphothreonine By similarity
Modified residue151Phosphotyrosine By similarity
Modified residue1611Phosphothreonine; by CAK

Experimental info

Mutagenesis1611T → A: Loss of phosphorylation by CDKD-1. Ref.8

Sequences

Sequence LengthMass (Da)Tools
P29618 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 51322D93AEF4C131

FASTA29434,071
        10         20         30         40         50         60 
MEQYEKEEKI GEGTYGVVYR ARDKVTNETI ALKKIRLEQE DEGVPSTAIR EISLLKEMHH 

        70         80         90        100        110        120 
GNIVRLHDVI HSEKRIYLVF EYLDLDLKKF MDSCPEFAKN PTLIKSYLYQ ILRGVAYCHS 

       130        140        150        160        170        180 
HRVLHRDLKP QNLLIDRRTN ALKLADFGLA RAFGIPVRTF THEVVTLWYR APEILLGSRQ 

       190        200        210        220        230        240 
YSTPVDMWSV GCIFAEMVNQ KPLFPGDSEI DELFKIFRVL GTPNEQSWPG VSSLPDYKSA 

       250        260        270        280        290 
FPKWQAQDLA TIVPTLDPAG LDLLSKMLRY EPNKRITARQ ALEHEYFKDL EMVQ 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of cDNA clones encoding cdc2 homologues from Oryza sativa: a functional homologue and cognate variants."
Hashimoto J., Hirabayashi T., Hayano Y., Hata S., Ohashi Y., Suzuka I., Utsugi T., Toh-e A., Kikuchi Y.
Mol. Gen. Genet. 233:10-16(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Nipponbare.
[2]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"Differential expression of a CAK (cdc2-activating kinase)-like protein kinase, cyclins and cdc2 genes from rice during the cell cycle and in response to gibberellin."
Sauter M.
Plant J. 11:181-190(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[6]"Adventitious root growth and cell-cycle induction in deepwater rice."
Lorbiecke R., Sauter M.
Plant Physiol. 119:21-30(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Differential expression of genes for cyclin-dependent protein kinases in rice plants."
Umeda M., Umeda-Hara C., Yamaguchi M., Hashimoto J., Uchimiya H.
Plant Physiol. 119:31-40(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"Activation of CDK-activating kinase is dependent on interaction with H-type cyclins in plants."
Yamaguchi M., Fabian T., Sauter M., Bhalerao R.P., Schrader J., Sandberg G., Umeda M., Uchimiya H.
Plant J. 24:11-20(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, MUTAGENESIS OF THR-161.
[9]"Genome-wide identification and expression analysis of rice cell cycle genes."
Guo J., Song J., Wang F., Zhang X.S.
Plant Mol. Biol. 64:349-360(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION AND GENE FAMILY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60374 mRNA. Translation: CAA42922.1.
DP000009 Genomic DNA. Translation: ABF93669.1.
AP008209 Genomic DNA. Translation: BAF10686.1.
PIRS22440.
RefSeqNP_001048772.1. NM_001055307.1.
UniGeneOs.11723.

3D structure databases

ProteinModelPortalP29618.
SMRP29618. Positions 1-289.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP29618.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4331415.
KEGGosa:4331415.

Organism-specific databases

GrameneP29618.

Phylogenomic databases

eggNOGCOG0515.
KOK02206.
ProtClustDBPLN00009.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDKA1_ORYSJ
AccessionPrimary (citable) accession number: P29618
Secondary accession number(s): Q10SL8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations