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Protein

Cyclin-dependent kinase A-1

Gene

CDKA-1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase A-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKA;1
Alternative name(s):
CDC2Os-1
Cell division control protein 2 homolog 1
Gene namesi
Name:CDKA-1
Synonyms:CDC2-1
Ordered Locus Names:Os03g0118400, LOC_Os03g02680
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 3

Organism-specific databases

GrameneiP29618.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi161 – 1611T → A: Loss of phosphorylation by CDKD-1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294Cyclin-dependent kinase A-1PRO_0000085755Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphothreonineBy similarity
Modified residuei15 – 151PhosphotyrosineBy similarity
Modified residuei161 – 1611Phosphothreonine; by CAK1 Publication

Post-translational modificationi

Phosphorylated at Thr-161 by CDKD-1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP29618.

Expressioni

Tissue specificityi

Expressed in the dividing region of the root apex and in differentiated cells such as those in the sclerenchyma, pericycle and parenchyma of the central cylinder.1 Publication

Developmental stagei

Expressed throughout the cell cycle.1 Publication

Inductioni

By submergence. Down-regulated by cytokinin.2 Publications

Structurei

3D structure databases

ProteinModelPortaliP29618.
SMRiP29618. Positions 1-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 287284Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiP29618.
KOiK02206.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQYEKEEKI GEGTYGVVYR ARDKVTNETI ALKKIRLEQE DEGVPSTAIR
60 70 80 90 100
EISLLKEMHH GNIVRLHDVI HSEKRIYLVF EYLDLDLKKF MDSCPEFAKN
110 120 130 140 150
PTLIKSYLYQ ILRGVAYCHS HRVLHRDLKP QNLLIDRRTN ALKLADFGLA
160 170 180 190 200
RAFGIPVRTF THEVVTLWYR APEILLGSRQ YSTPVDMWSV GCIFAEMVNQ
210 220 230 240 250
KPLFPGDSEI DELFKIFRVL GTPNEQSWPG VSSLPDYKSA FPKWQAQDLA
260 270 280 290
TIVPTLDPAG LDLLSKMLRY EPNKRITARQ ALEHEYFKDL EMVQ
Length:294
Mass (Da):34,071
Last modified:April 1, 1993 - v1
Checksum:i51322D93AEF4C131
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60374 mRNA. Translation: CAA42922.1.
DP000009 Genomic DNA. Translation: ABF93669.1.
AP008209 Genomic DNA. Translation: BAF10686.1.
PIRiS22440.
RefSeqiNP_001048772.1. NM_001055307.1.
UniGeneiOs.11723.

Genome annotation databases

GeneIDi4331415.
KEGGiosa:4331415.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60374 mRNA. Translation: CAA42922.1.
DP000009 Genomic DNA. Translation: ABF93669.1.
AP008209 Genomic DNA. Translation: BAF10686.1.
PIRiS22440.
RefSeqiNP_001048772.1. NM_001055307.1.
UniGeneiOs.11723.

3D structure databases

ProteinModelPortaliP29618.
SMRiP29618. Positions 1-289.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP29618.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4331415.
KEGGiosa:4331415.

Organism-specific databases

GrameneiP29618.

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiP29618.
KOiK02206.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of cDNA clones encoding cdc2 homologues from Oryza sativa: a functional homologue and cognate variants."
    Hashimoto J., Hirabayashi T., Hayano Y., Hata S., Ohashi Y., Suzuka I., Utsugi T., Toh-e A., Kikuchi Y.
    Mol. Gen. Genet. 233:10-16(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
  2. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Differential expression of a CAK (cdc2-activating kinase)-like protein kinase, cyclins and cdc2 genes from rice during the cell cycle and in response to gibberellin."
    Sauter M.
    Plant J. 11:181-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "Adventitious root growth and cell-cycle induction in deepwater rice."
    Lorbiecke R., Sauter M.
    Plant Physiol. 119:21-30(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Differential expression of genes for cyclin-dependent protein kinases in rice plants."
    Umeda M., Umeda-Hara C., Yamaguchi M., Hashimoto J., Uchimiya H.
    Plant Physiol. 119:31-40(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Activation of CDK-activating kinase is dependent on interaction with H-type cyclins in plants."
    Yamaguchi M., Fabian T., Sauter M., Bhalerao R.P., Schrader J., Sandberg G., Umeda M., Uchimiya H.
    Plant J. 24:11-20(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, MUTAGENESIS OF THR-161.
  9. "Genome-wide identification and expression analysis of rice cell cycle genes."
    Guo J., Song J., Wang F., Zhang X.S.
    Plant Mol. Biol. 64:349-360(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION AND GENE FAMILY.

Entry informationi

Entry nameiCDKA1_ORYSJ
AccessioniPrimary (citable) accession number: P29618
Secondary accession number(s): Q10SL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: January 7, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.