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P29603 (FER_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ferredoxin
Gene names
Name:fdxA
Ordered Locus Names:PF1909
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length67 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.

Cofactor

Binds 1 4Fe-4S cluster which readily converts to a stable 3Fe-4S form.

Subunit structure

Homodimer Potential. Ref.15

Sequence similarities

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 6766Ferredoxin
PRO_0000159199

Regions

Domain3 – 31294Fe-4S ferredoxin-type 1
Domain36 – 67324Fe-4S ferredoxin-type 2

Sites

Metal binding121Iron-sulfur (4Fe-4S)
Metal binding151Iron-sulfur (4Fe-4S)
Metal binding181Iron-sulfur (4Fe-4S)
Metal binding571Iron-sulfur (4Fe-4S)

Amino acid modifications

Disulfide bond22 ↔ 49 Ref.10 Ref.11

Experimental info

Mutagenesis151D → C: No effect on the efficiency of electron transfer. Ref.3 Ref.5 Ref.12 Ref.13
Mutagenesis151D → S: The efficiency of electron transfer is compromised. Ref.3 Ref.5 Ref.12 Ref.13
Mutagenesis341A → C: The mixed spin cluster is converted into a homogeneous spin cluster (S=3/2). Ref.4 Ref.5 Ref.6

Secondary structure

................ 67
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29603 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: AF9B062690B34420

FASTA677,298
        10         20         30         40         50         60 
MAWKVSVDQD TCIGDAICAS LCPDVFEMND EGKAQPKVEV IEDEELYNCA KEAMEACPVS 


AITIEEA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, and molecular characterization of the gene encoding an extremely thermostable [4Fe-4S] ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus."
Heltzel A., Smith E.T., Zhou Z.H., Blamey J.M., Adams M.W.W.
J. Bacteriol. 176:4790-4793(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]"Site-directed mutations of the 4Fe-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus: role of the cluster-coordinating aspartate in physiological electron transfer reactions."
Zhou Z.H., Adams M.W.W.
Biochemistry 36:10892-10900(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ASP-15, CIRCULAR DICHROISM ANALYSIS, EPR SPECTROSCOPY.
[4]"Spectroscopic evidence for a new type of [Fe3S4] cluster in a mutant form of Pyrococcus furiosus ferredoxin."
Duderstadt R.E., Brereton P.S., Adams M.W.W., Johnson M.K.
J. Am. Chem. Soc. 120:8525-8526(1998)
Cited for: MUTAGENESIS OF ALA-34, MAGNETIC CIRCULAR DICHROISM, EPR SPECTROSCOPY, RESONANCE RAMAN SPECTROSCOPY OF (3FE--4S) FORM.
[5]"Effect of serinate ligation at each of the iron sites of the [Fe4S4] cluster of Pyrococcus furiosus ferredoxin on the redox, spectroscopic, and biological properties."
Brereton P.S., Duderstadt R.E., Staples C.R., Johnson M.K., Adams M.W.W.
Biochemistry 38:10594-10605(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF CYSTEINE RESIDUES, MAGNETIC CIRCULAR DICHROISM, EPR SPECTROSCOPY, RESONANCE RAMAN SPECTROSCOPY.
[6]"A pure S=3/2 [Fe4S4]+ cluster in the A33Y variant of Pyrococcus furiosus ferredoxin."
Duderstadt R.E., Brereton P.S., Adams M.W.W., Johnson M.K.
FEBS Lett. 454:21-26(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ALA-34, MAGNETIC CIRCULAR DICHROISM, EPR SPECTROSCOPY, RESONANCE RAMAN SPECTROSCOPY.
[7]"Proton NMR investigation of the oxidized three-iron clusters in the ferredoxins from the hyperthermophilic archae Pyrococcus furiosus and Thermococcus litoralis."
Busse S.C., la Mar G.N., Yu L.P., Howard J.B., Smith E.T., Zhou Z.H., Adams M.W.W.
Biochemistry 31:11952-11962(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[8]"Solution 1H NMR determination of secondary structure for the three-iron form of ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus."
Teng Q., Zhou Z.H., Smith E.T., Busse S.C., Howard J.B., Adams M.W.W., La Mar G.N.
Biochemistry 33:6316-6326(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 3FE--4S FORM.
[9]"1H NMR investigation of the electronic and molecular structure of the four-iron cluster ferredoxin from the hyperthermophile Pyrococcus furiosus. Identification of Asp 14 as a cluster ligand in each of the four redox states."
Calzolai L., Gorst C.M., Zhou Z.H., Teng Q., Adams M.W.W., La Mar G.N.
Biochemistry 34:11373-11384(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[10]"1H NMR investigation of the paramagnetic cluster environment in Pyrococcus furiosus three-iron ferredoxin: sequence-specific assignment of ligated cysteines independent of tertiary structure."
Gorst C.M., Yeh Y.-H., Teng Q., Calzolai L., Zhou Z.H., Adams M.W.W., La Mar G.N.
Biochemistry 34:600-610(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 3FE--4S FORM.
[11]"Participation of the disulfide bridge in the redox cycle of the ferredoxin from the hyperthermophile Pyrococcus furiosus: 1H nuclear magnetic resonance time resolution of the four redox states at ambient temperature."
Gorst C.M., Zhou Z.H., Teng Q., Howard J.B., Adams M.W.W., La Mar G.N.
Biochemistry 34:8788-8795(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR, DISULFIDE BOND.
[12]"Role of cluster-ligated aspartate in gating electron transfer in the four-iron ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus."
Calzolai L., Zhou Z.H., Adams M.W.W., La Mar G.N.
J. Am. Chem. Soc. 118:2513-2514(1996)
Cited for: STRUCTURE BY NMR, MUTAGENESIS OF ASP-15.
[13]"Solution NMR study of the electronic structure and magnetic properties of cluster ligation mutants of the four-iron ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus."
Calzolai L., Gorst C.M., Bren K.L., Zhou Z.H., Adams M.W.W., La Mar G.N.
J. Am. Chem. Soc. 119:9341-9350(1997)
Cited for: STRUCTURE BY NMR, MUTAGENESIS OF ASP-15.
[14]"Solution NMR characterization of the thermodynamics of the disulfide bond orientational isomerism and its effect of cluster electronic properties for the hyperthermostable three-iron cluster ferredoxin from the archaeon Pyrococcus furiosus."
Webba da Silva M., Sham S., Gorst C.M., Calzolai L., Brereton P.S., Adams M.W.W., La Mar G.N.
Biochemistry 40:12575-12583(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 3FE--4S FORM.
[15]"The 1.5 A resolution crystal structure of [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus."
Nielsen M.S., Harris P., Ooi B.L., Christensen H.E.
Biochemistry 43:5188-5194(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR CLUSTER, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79502 Genomic DNA. Translation: CAA56040.1.
AE009950 Genomic DNA. Translation: AAL82033.1.
PIRA44203.
RefSeqNP_579638.1. NC_003413.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SIZX-ray2.25A/C2-67[»]
1SJ1X-ray1.50A/B2-67[»]
2Z8QX-ray1.70A/B2-67[»]
3PNIX-ray2.80A/B2-67[»]
4DHVX-ray1.95A/B2-67[»]
ProteinModelPortalP29603.
SMRP29603. Positions 2-67.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING186497.PF1909.

Proteomic databases

PRIDEP29603.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL82033; AAL82033; PF1909.
GeneID1469789.
KEGGpfu:PF1909.

Phylogenomic databases

eggNOGCOG1141.
HOGENOMHOG000044619.
KOK05337.
OMAANIYRSC.

Family and domain databases

InterProIPR001080. 3Fe4S_ferredoxin.
IPR017896. 4Fe4S_Fe-S-bd.
[Graphical view]
PRINTSPR00352. 3FE4SFRDOXIN.
PROSITEPS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP29603.

Entry information

Entry nameFER_PYRFU
AccessionPrimary (citable) accession number: P29603
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references