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Protein

Subtilisin Savinase

Gene
N/A
Organism
Bacillus lentus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.

Catalytic activityi

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2Calcium 11
Active sitei32Charge relay system1
Metal bindingi40Calcium 11
Active sitei62Charge relay system1
Metal bindingi73Calcium 1; via carbonyl oxygen1
Metal bindingi75Calcium 11
Metal bindingi77Calcium 1; via carbonyl oxygen1
Metal bindingi79Calcium 1; via carbonyl oxygen1
Metal bindingi163Calcium 2; via carbonyl oxygen1
Metal bindingi165Calcium 2; via carbonyl oxygen1
Metal bindingi168Calcium 2; via carbonyl oxygen1
Active sitei215Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Sporulation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.21.62. 668.

Protein family/group databases

MEROPSiS08.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin Savinase (EC:3.4.21.62)
Alternative name(s):
Alkaline protease
OrganismiBacillus lentus
Taxonomic identifieri1467 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Biotechnological usei

Used as a detergent protease. Sold under the name Savinase by Novozymes.

Protein family/group databases

Allergomei903. Bac l Subtilisin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000764181 – 269Subtilisin SavinaseAdd BLAST269

Structurei

Secondary structure

1269
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 10Combined sources5
Helixi13 – 18Combined sources6
Beta strandi27 – 33Combined sources7
Beta strandi43 – 48Combined sources6
Beta strandi55 – 57Combined sources3
Beta strandi59 – 61Combined sources3
Helixi62 – 71Combined sources10
Beta strandi75 – 78Combined sources4
Beta strandi87 – 92Combined sources6
Beta strandi98 – 100Combined sources3
Helixi102 – 114Combined sources13
Beta strandi118 – 122Combined sources5
Beta strandi126 – 128Combined sources3
Helixi131 – 142Combined sources12
Beta strandi146 – 150Combined sources5
Beta strandi156 – 158Combined sources3
Turni162 – 164Combined sources3
Beta strandi168 – 174Combined sources7
Beta strandi178 – 180Combined sources3
Beta strandi190 – 195Combined sources6
Beta strandi197 – 203Combined sources7
Turni204 – 206Combined sources3
Beta strandi207 – 211Combined sources5
Helixi214 – 231Combined sources18
Helixi237 – 246Combined sources10
Helixi254 – 257Combined sources4
Helixi264 – 267Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9JX-ray1.80A1-267[»]
1C9MX-ray1.67A1-269[»]
1C9NX-ray1.50A1-267[»]
1GCIX-ray0.78A1-269[»]
1IAVX-ray1.80A1-269[»]
1JEAX-ray2.00A1-269[»]
1NDQX-ray1.80A1-269[»]
1NDUX-ray1.60A1-269[»]
1Q5PX-ray1.60A1-269[»]
1SVNX-ray1.40A1-269[»]
1TK2X-ray1.54A1-263[»]
3BX1X-ray1.85A/B1-269[»]
4CFYX-ray1.17A1-269[»]
4CFZX-ray1.57A1-269[»]
4CG0X-ray1.36A1-269[»]
5AQEX-ray1.10A1-269[»]
5ARBX-ray1.15A1-269[»]
5ARCX-ray1.10A1-269[»]
5ARDX-ray1.55A1-269[»]
ProteinModelPortaliP29600.
SMRiP29600.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29600.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 268Peptidase S8Add BLAST242

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29600-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AQSVPWGISR VQAPAAHNRG LTGSGVKVAV LDTGISTHPD LNIRGGASFV
60 70 80 90 100
PGEPSTQDGN GHGTHVAGTI AALNNSIGVL GVAPSAELYA VKVLGASGSG
110 120 130 140 150
SVSSIAQGLE WAGNNGMHVA NLSLGSPSPS ATLEQAVNSA TSRGVLVVAA
160 170 180 190 200
SGNSGAGSIS YPARYANAMA VGATDQNNNR ASFSQYGAGL DIVAPGVNVQ
210 220 230 240 250
STYPGSTYAS LNGTSMATPH VAGAAALVKQ KNPSWSNVQI RNHLKNTATS
260
LGSTNLYGSG LVNAEAATR
Length:269
Mass (Da):26,698
Last modified:April 1, 1993 - v1
Checksum:i4D89F8778999BF8D
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9JX-ray1.80A1-267[»]
1C9MX-ray1.67A1-269[»]
1C9NX-ray1.50A1-267[»]
1GCIX-ray0.78A1-269[»]
1IAVX-ray1.80A1-269[»]
1JEAX-ray2.00A1-269[»]
1NDQX-ray1.80A1-269[»]
1NDUX-ray1.60A1-269[»]
1Q5PX-ray1.60A1-269[»]
1SVNX-ray1.40A1-269[»]
1TK2X-ray1.54A1-263[»]
3BX1X-ray1.85A/B1-269[»]
4CFYX-ray1.17A1-269[»]
4CFZX-ray1.57A1-269[»]
4CG0X-ray1.36A1-269[»]
5AQEX-ray1.10A1-269[»]
5ARBX-ray1.15A1-269[»]
5ARCX-ray1.10A1-269[»]
5ARDX-ray1.55A1-269[»]
ProteinModelPortaliP29600.
SMRiP29600.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei903. Bac l Subtilisin.
MEROPSiS08.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.4.21.62. 668.

Miscellaneous databases

EvolutionaryTraceiP29600.

Family and domain databases

Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSUBS_BACLE
AccessioniPrimary (citable) accession number: P29600
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.