Reviewed, UniProtKB/Swiss-Prot P29598 (UROK_RAT)

Names and origin

Protein names

Recommended name:
    Urokinase-type plasminogen activator
      Short name=uPA
      Short name=U-plasminogen activator
    EC=3.4.21.73
Cleaved into the following 3 chains:
    1- Recommended name:
            Urokinase-type plasminogen activator long chain A
    2- Recommended name:
            Urokinase-type plasminogen activator short chain A
    3- Recommended name:
            Urokinase-type plasminogen activator chain B

Gene names

Name:

Plau

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
Catalytic activity	Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Subunit structure	Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 EGF-like domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Plasminogen activation
Cellular component	Secreted
Domain	EGF-like domain Kringle Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	angiogenesis Inferred from mutant phenotype. Source: RGD chemotaxis Traceable author statement. Source: RGD embryo implantation Inferred from expression pattern. Source: RGD proteolysis Traceable author statement. Source: RGD signal transduction Traceable author statement. Source: RGD skeletal muscle tissue regeneration Inferred from mutant phenotype. Source: RGD
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 432	413	Urokinase-type plasminogen activator		PRO_0000028333
	Chain	20 – 177	158	Urokinase-type plasminogen activator long chain A By similarity		PRO_0000028334
	Chain	156 – 177	22	Urokinase-type plasminogen activator short chain A By similarity		PRO_0000028335
	Chain	179 – 432	254	Urokinase-type plasminogen activator chain B By similarity		PRO_0000028336
Regions
	Domain	27 – 63	37	EGF-like
	Domain	70 – 151	82	Kringle
	Domain	179 – 425	247	Peptidase S1
	Region	34 – 57	24	Binds urokinase plasminogen activator surface receptor
	Region	152 – 178	27	Connecting peptide
Sites
	Active site	225	1	Charge relay system
	Active site	276	1	Charge relay system
	Active site	377	1	Charge relay system
Amino acid modifications
	Disulfide bond	31 ↔ 39		By similarity
	Disulfide bond	33 ↔ 51		By similarity
	Disulfide bond	53 ↔ 62		By similarity
	Disulfide bond	70 ↔ 151		By similarity
	Disulfide bond	91 ↔ 133		By similarity
	Disulfide bond	122 ↔ 146		By similarity
	Disulfide bond	168 ↔ 300		Interchain (between A and B chains) By similarity
	Disulfide bond	210 ↔ 226		By similarity
	Disulfide bond	218 ↔ 289		By similarity
	Disulfide bond	314 ↔ 383		By similarity
	Disulfide bond	346 ↔ 362		By similarity
	Disulfide bond	373 ↔ 401		By similarity
Experimental info
	Sequence conflict	16	1	N → H in CAA46601. Ref.2
	Sequence conflict	24	1	E → G in CAA46601. Ref.2
	Sequence conflict	332	1	D → N in CAA46601. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P29598-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 4EB1B96C716244C8	FASTA	432	47,957
	10 20 30 40 50 60 MRVWLASLFL CALVANSEGG SELEASDESN CGCQNGGVCV SYKYFSSIRR CSCPKKFKGE 70 80 90 100 110 120 HCEIDTSKTC YHGNGQSYRG KANTDTKGRP CLAWNSPAVL QQTYNAHRSD ALSLGLGKHN 130 140 150 160 170 180 YCRNPDNQRR PWCYVQIGLK QFVQECMVQD CSLSKKPSST VDQQGFQCGQ KALRPRFKIV 190 200 210 220 230 240 GGEFTVVENQ PWFAAIYLKN KGGSPPSFKC GGSLISPCWV ASATHCFVNQ PKKEEYVVYL 250 260 270 280 290 300 GQSKRNSYNP GEMKFEVEQL ILHEDFSDET LAFHNDIALL KIRTSTGQCA QPSRTIQTIC 310 320 330 340 350 360 LPPRFGDAPF GSDCEITGFG QESATDYFYP KDLKMSVVKI ISHEQCKQPH YYGSEINYKM 370 380 390 400 410 420 LCAADPEWKT DSCSGDSGGP LICNIDGRPT LSGIVSWGSG CAEKNKPGVY TRVSYFLNWI 430 QSHIGEENGL AF

References

[1]	"Transcriptional and posttranscriptional activation of urokinase plasminogen activator gene expression in metastatic tumor cells." Henderson B.R., Tansey W.P., Phillips S.M., Ramshaw I.A., Kefford R.F. Cancer Res. 52:2489-2496(1992) [PubMed: 1568219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344.
[2]	Rabbani S.A. Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[3]	"The receptor for the plasminogen activator of urokinase type is up-regulated in transformed rat thyroid cells." Ragno P., Cassano S., Degen J., Kessler C., Blasi F., Rossi G. FEBS Lett. 306:193-198(1992) [PubMed: 1321734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-62, IDENTIFICATION OF UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR BINDING DOMAIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63434 mRNA. Translation: CAA45028.1. X65651 mRNA. Translation: CAA46601.1. X66907 Genomic DNA. Translation: CAA47356.1.
IPI	IPI00205838.
PIR	S18932. S24604.
UniGene	Rn.6064
3D structure databases
SMR	P29598. Positions 29-155, 166-427.
ModBase	Search...
Protein-protein interaction databases
STRING	P29598.
Protein family/group databases
MEROPS	S01.231.
PTM databases
PhosphoSite	P29598.
Genome annotation databases
Ensembl	ENSRNOT00000014273; ENSRNOP00000014273; ENSRNOG00000010516; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	3343. Plau.
Phylogenomic databases
eggNOG	roNOG07876.
HOVERGEN	P29598.
InParanoid	P29598.
Enzyme and pathway databases
BRENDA	3.4.21.73. 248.
Gene expression databases
ArrayExpress	P29598.
Genevestigator	P29598.
GermOnline	ENSRNOG00000010516. Rattus norvegicus.
Family and domain databases
InterPro	IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR000001. Kringle. IPR013806. Kringle-like. IPR018056. Kringle_CS. IPR018059. Kringle_sub. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Gene3D	G3DSA:2.40.20.10. Kringle. 1 hit.
Pfam	PF00051. Kringle. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN. PR00018. KRINGLE.
SMART	SM00181. EGF. 1 hit. SM00130. KR. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. False negative. PS50026. EGF_3. 1 hit. PS00021. KRINGLE_1. 1 hit. PS50070. KRINGLE_2. 1 hit. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UROK_RAT

Accession

Primary (citable) accession number: P29598
Secondary accession number(s): Q6LBK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 19, 2010
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)