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P29598

- UROK_RAT

UniProt

P29598 - UROK_RAT

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Protein

Urokinase-type plasminogen activator

Gene

Plau

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei225 – 2251Charge relay system
Active sitei276 – 2761Charge relay system
Active sitei377 – 3771Charge relay system

GO - Molecular functioni

  1. peptidase activity Source: RGD
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. angiogenesis Source: RGD
  2. cellular response to fluid shear stress Source: RGD
  3. cellular response to glucose stimulus Source: RGD
  4. cellular response to hepatocyte growth factor stimulus Source: RGD
  5. cellular response to hypoxia Source: RGD
  6. cellular response to lipopolysaccharide Source: RGD
  7. cellular response to organic substance Source: RGD
  8. cellular response to staurosporine Source: RGD
  9. embryo implantation Source: RGD
  10. neuron death Source: RGD
  11. positive regulation of cell proliferation Source: RGD
  12. positive regulation of ovulation Source: RGD
  13. positive regulation of reactive oxygen species metabolic process Source: RGD
  14. positive regulation of smooth muscle cell migration Source: RGD
  15. regulation of hepatocyte proliferation Source: RGD
  16. response to activity Source: RGD
  17. response to hepatocyte growth factor Source: RGD
  18. response to hyperoxia Source: RGD
  19. skeletal muscle tissue regeneration Source: RGD
  20. spermatogenesis Source: RGD
  21. wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Plasminogen activation

Protein family/group databases

MEROPSiS01.231.

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:Plau
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3343. Plau.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular space Source: RGD
  2. membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 432413Urokinase-type plasminogen activatorPRO_0000028333Add
BLAST
Chaini20 – 177158Urokinase-type plasminogen activator long chain ABy similarityPRO_0000028334Add
BLAST
Chaini156 – 17722Urokinase-type plasminogen activator short chain ABy similarityPRO_0000028335Add
BLAST
Chaini179 – 432254Urokinase-type plasminogen activator chain BBy similarityPRO_0000028336Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 39By similarity
Disulfide bondi33 ↔ 51By similarity
Disulfide bondi53 ↔ 62By similarity
Disulfide bondi70 ↔ 151By similarity
Disulfide bondi91 ↔ 133By similarity
Disulfide bondi122 ↔ 146By similarity
Modified residuei158 – 1581PhosphoserineBy similarity
Disulfide bondi168 ↔ 300Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondi210 ↔ 226By similarity
Disulfide bondi218 ↔ 289By similarity
Disulfide bondi314 ↔ 383By similarity
Disulfide bondi346 ↔ 362By similarity
Disulfide bondi373 ↔ 401By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP29598.
PRIDEiP29598.

PTM databases

PhosphoSiteiP29598.

Expressioni

Gene expression databases

GenevestigatoriP29598.

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP29598.
SMRiP29598. Positions 29-155, 166-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 6337EGF-likePROSITE-ProRule annotationAdd
BLAST
Domaini70 – 15182KringlePROSITE-ProRule annotationAdd
BLAST
Domaini179 – 425247Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 5724Binds urokinase plasminogen activator surface receptorAdd
BLAST
Regioni152 – 17827Connecting peptideAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 kringle domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000237314.
HOVERGENiHBG008633.
InParanoidiP29598.
PhylomeDBiP29598.

Family and domain databases

Gene3Di2.40.20.10. 1 hit.
InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00181. EGF. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29598-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVWLASLFL CALVANSEGG SELEASDESN CGCQNGGVCV SYKYFSSIRR
60 70 80 90 100
CSCPKKFKGE HCEIDTSKTC YHGNGQSYRG KANTDTKGRP CLAWNSPAVL
110 120 130 140 150
QQTYNAHRSD ALSLGLGKHN YCRNPDNQRR PWCYVQIGLK QFVQECMVQD
160 170 180 190 200
CSLSKKPSST VDQQGFQCGQ KALRPRFKIV GGEFTVVENQ PWFAAIYLKN
210 220 230 240 250
KGGSPPSFKC GGSLISPCWV ASATHCFVNQ PKKEEYVVYL GQSKRNSYNP
260 270 280 290 300
GEMKFEVEQL ILHEDFSDET LAFHNDIALL KIRTSTGQCA QPSRTIQTIC
310 320 330 340 350
LPPRFGDAPF GSDCEITGFG QESATDYFYP KDLKMSVVKI ISHEQCKQPH
360 370 380 390 400
YYGSEINYKM LCAADPEWKT DSCSGDSGGP LICNIDGRPT LSGIVSWGSG
410 420 430
CAEKNKPGVY TRVSYFLNWI QSHIGEENGL AF
Length:432
Mass (Da):47,957
Last modified:April 1, 1993 - v1
Checksum:i4EB1B96C716244C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161N → H in CAA46601. 1 PublicationCurated
Sequence conflicti24 – 241E → G in CAA46601. 1 PublicationCurated
Sequence conflicti332 – 3321D → N in CAA46601. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63434 mRNA. Translation: CAA45028.1.
X65651 mRNA. Translation: CAA46601.1.
X66907 Genomic DNA. Translation: CAA47356.1.
PIRiS24604. S18932.
UniGeneiRn.6064.

Genome annotation databases

UCSCiRGD:3343. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63434 mRNA. Translation: CAA45028.1 .
X65651 mRNA. Translation: CAA46601.1 .
X66907 Genomic DNA. Translation: CAA47356.1 .
PIRi S24604. S18932.
UniGenei Rn.6064.

3D structure databases

ProteinModelPortali P29598.
SMRi P29598. Positions 29-155, 166-427.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P29598.
ChEMBLi CHEMBL1075245.

Protein family/group databases

MEROPSi S01.231.

PTM databases

PhosphoSitei P29598.

Proteomic databases

PaxDbi P29598.
PRIDEi P29598.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:3343. rat.

Organism-specific databases

RGDi 3343. Plau.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000237314.
HOVERGENi HBG008633.
InParanoidi P29598.
PhylomeDBi P29598.

Miscellaneous databases

PROi P29598.

Gene expression databases

Genevestigatori P29598.

Family and domain databases

Gene3Di 2.40.20.10. 1 hit.
InterProi IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00181. EGF. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEi PS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Transcriptional and posttranscriptional activation of urokinase plasminogen activator gene expression in metastatic tumor cells."
    Henderson B.R., Tansey W.P., Phillips S.M., Ramshaw I.A., Kefford R.F.
    Cancer Res. 52:2489-2496(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer 344.
  2. Rabbani S.A.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  3. "The receptor for the plasminogen activator of urokinase type is up-regulated in transformed rat thyroid cells."
    Ragno P., Cassano S., Degen J., Kessler C., Blasi F., Rossi G.
    FEBS Lett. 306:193-198(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-62, IDENTIFICATION OF UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR BINDING DOMAIN.

Entry informationi

Entry nameiUROK_RAT
AccessioniPrimary (citable) accession number: P29598
Secondary accession number(s): Q6LBK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 26, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3