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Protein

Urokinase-type plasminogen activator

Gene

Plau

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei225Charge relay system1
Active sitei276Charge relay system1
Active sitei377Charge relay system1

GO - Molecular functioni

  • peptidase activity Source: RGD
  • serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

  • angiogenesis Source: RGD
  • cellular response to fluid shear stress Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to hepatocyte growth factor stimulus Source: RGD
  • cellular response to hypoxia Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • cellular response to organic substance Source: RGD
  • cellular response to staurosporine Source: RGD
  • embryo implantation Source: RGD
  • fibrinolysis Source: RGD
  • neuron death Source: RGD
  • plasminogen activation Source: RGD
  • positive regulation of cell migration Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of ovulation Source: RGD
  • positive regulation of reactive oxygen species metabolic process Source: RGD
  • positive regulation of smooth muscle cell migration Source: RGD
  • regulation of cell adhesion mediated by integrin Source: RGD
  • regulation of cell proliferation Source: RGD
  • regulation of hepatocyte proliferation Source: RGD
  • regulation of receptor activity Source: RGD
  • regulation of smooth muscle cell-matrix adhesion Source: RGD
  • regulation of smooth muscle cell migration Source: RGD
  • response to activity Source: RGD
  • response to hepatocyte growth factor Source: RGD
  • response to hyperoxia Source: RGD
  • response to hypoxia Source: RGD
  • skeletal muscle tissue regeneration Source: RGD
  • smooth muscle cell migration Source: RGD
  • spermatogenesis Source: RGD
  • wound healing Source: RGD

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processPlasminogen activation

Protein family/group databases

MEROPSiS01.231

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:Plau
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3343 Plau

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075245

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002833320 – 432Urokinase-type plasminogen activatorAdd BLAST413
ChainiPRO_000002833420 – 177Urokinase-type plasminogen activator long chain ABy similarityAdd BLAST158
ChainiPRO_0000028335156 – 177Urokinase-type plasminogen activator short chain ABy similarityAdd BLAST22
ChainiPRO_0000028336179 – 432Urokinase-type plasminogen activator chain BBy similarityAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 39By similarity
Disulfide bondi33 ↔ 51By similarity
Disulfide bondi53 ↔ 62By similarity
Disulfide bondi70 ↔ 151By similarity
Disulfide bondi91 ↔ 133By similarity
Disulfide bondi122 ↔ 146By similarity
Modified residuei158PhosphoserineBy similarity1
Disulfide bondi168 ↔ 300Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondi210 ↔ 226By similarity
Disulfide bondi218 ↔ 289By similarity
Disulfide bondi314 ↔ 383By similarity
Disulfide bondi346 ↔ 362By similarity
Disulfide bondi373 ↔ 401By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP29598
PRIDEiP29598

PTM databases

PhosphoSitePlusiP29598

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014273

Chemistry databases

BindingDBiP29598

Structurei

3D structure databases

ProteinModelPortaliP29598
SMRiP29598
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 63EGF-likePROSITE-ProRule annotationAdd BLAST37
Domaini70 – 151KringlePROSITE-ProRule annotationAdd BLAST82
Domaini179 – 425Peptidase S1PROSITE-ProRule annotationAdd BLAST247

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 57Binds urokinase plasminogen activator surface receptorAdd BLAST24
Regioni152 – 178Connecting peptideAdd BLAST27

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiENOG410IGFI Eukaryota
COG5640 LUCA
HOGENOMiHOG000237314
HOVERGENiHBG008633
InParanoidiP29598
PhylomeDBiP29598

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR033116 TRYPSIN_SER
IPR034814 Urokinase
PANTHERiPTHR24264:SF38 PTHR24264:SF38, 1 hit
PfamiView protein in Pfam
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVWLASLFL CALVANSEGG SELEASDESN CGCQNGGVCV SYKYFSSIRR
60 70 80 90 100
CSCPKKFKGE HCEIDTSKTC YHGNGQSYRG KANTDTKGRP CLAWNSPAVL
110 120 130 140 150
QQTYNAHRSD ALSLGLGKHN YCRNPDNQRR PWCYVQIGLK QFVQECMVQD
160 170 180 190 200
CSLSKKPSST VDQQGFQCGQ KALRPRFKIV GGEFTVVENQ PWFAAIYLKN
210 220 230 240 250
KGGSPPSFKC GGSLISPCWV ASATHCFVNQ PKKEEYVVYL GQSKRNSYNP
260 270 280 290 300
GEMKFEVEQL ILHEDFSDET LAFHNDIALL KIRTSTGQCA QPSRTIQTIC
310 320 330 340 350
LPPRFGDAPF GSDCEITGFG QESATDYFYP KDLKMSVVKI ISHEQCKQPH
360 370 380 390 400
YYGSEINYKM LCAADPEWKT DSCSGDSGGP LICNIDGRPT LSGIVSWGSG
410 420 430
CAEKNKPGVY TRVSYFLNWI QSHIGEENGL AF
Length:432
Mass (Da):47,957
Last modified:April 1, 1993 - v1
Checksum:i4EB1B96C716244C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16N → H in CAA46601 (Ref. 2) Curated1
Sequence conflicti24E → G in CAA46601 (Ref. 2) Curated1
Sequence conflicti332D → N in CAA46601 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63434 mRNA Translation: CAA45028.1
X65651 mRNA Translation: CAA46601.1
X66907 Genomic DNA Translation: CAA47356.1
PIRiS24604 S18932
UniGeneiRn.6064

Genome annotation databases

UCSCiRGD:3343 rat

Similar proteinsi

Entry informationi

Entry nameiUROK_RAT
AccessioniPrimary (citable) accession number: P29598
Secondary accession number(s): Q6LBK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: March 28, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health