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P29598

- UROK_RAT

UniProt

P29598 - UROK_RAT

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Protein
Urokinase-type plasminogen activator
Gene
Plau
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationi

Inhibited by SERPINA5 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei225 – 2251Charge relay system
Active sitei276 – 2761Charge relay system
Active sitei377 – 3771Charge relay system

GO - Molecular functioni

  1. peptidase activity Source: RGD
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. angiogenesis Source: RGD
  2. cellular response to fluid shear stress Source: RGD
  3. cellular response to glucose stimulus Source: RGD
  4. cellular response to hepatocyte growth factor stimulus Source: RGD
  5. cellular response to hypoxia Source: RGD
  6. cellular response to lipopolysaccharide Source: RGD
  7. cellular response to organic substance Source: RGD
  8. cellular response to staurosporine Source: RGD
  9. embryo implantation Source: RGD
  10. neuron death Source: RGD
  11. positive regulation of cell proliferation Source: RGD
  12. positive regulation of ovulation Source: RGD
  13. positive regulation of reactive oxygen species metabolic process Source: RGD
  14. positive regulation of smooth muscle cell migration Source: RGD
  15. regulation of hepatocyte proliferation Source: RGD
  16. response to activity Source: RGD
  17. response to hepatocyte growth factor Source: RGD
  18. response to hyperoxia Source: RGD
  19. skeletal muscle tissue regeneration Source: RGD
  20. spermatogenesis Source: RGD
  21. wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Plasminogen activation

Protein family/group databases

MEROPSiS01.231.

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:Plau
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3343. Plau.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular space Source: RGD
  2. membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 432413Urokinase-type plasminogen activator
PRO_0000028333Add
BLAST
Chaini20 – 177158Urokinase-type plasminogen activator long chain A By similarity
PRO_0000028334Add
BLAST
Chaini156 – 17722Urokinase-type plasminogen activator short chain A By similarity
PRO_0000028335Add
BLAST
Chaini179 – 432254Urokinase-type plasminogen activator chain B By similarity
PRO_0000028336Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 39 By similarity
Disulfide bondi33 ↔ 51 By similarity
Disulfide bondi53 ↔ 62 By similarity
Disulfide bondi70 ↔ 151 By similarity
Disulfide bondi91 ↔ 133 By similarity
Disulfide bondi122 ↔ 146 By similarity
Modified residuei158 – 1581Phosphoserine By similarity
Disulfide bondi168 ↔ 300Interchain (between A and B chains) By similarity
Disulfide bondi210 ↔ 226 By similarity
Disulfide bondi218 ↔ 289 By similarity
Disulfide bondi314 ↔ 383 By similarity
Disulfide bondi346 ↔ 362 By similarity
Disulfide bondi373 ↔ 401 By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP29598.
PRIDEiP29598.

PTM databases

PhosphoSiteiP29598.

Expressioni

Gene expression databases

GenevestigatoriP29598.

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.

Structurei

3D structure databases

ProteinModelPortaliP29598.
SMRiP29598. Positions 29-155, 166-427.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 6337EGF-like
Add
BLAST
Domaini70 – 15182Kringle
Add
BLAST
Domaini179 – 425247Peptidase S1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 5724Binds urokinase plasminogen activator surface receptor
Add
BLAST
Regioni152 – 17827Connecting peptide
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000237314.
HOVERGENiHBG008633.
InParanoidiP29598.
PhylomeDBiP29598.

Family and domain databases

Gene3Di2.40.20.10. 1 hit.
InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00181. EGF. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29598-1 [UniParc]FASTAAdd to Basket

« Hide

MRVWLASLFL CALVANSEGG SELEASDESN CGCQNGGVCV SYKYFSSIRR    50
CSCPKKFKGE HCEIDTSKTC YHGNGQSYRG KANTDTKGRP CLAWNSPAVL 100
QQTYNAHRSD ALSLGLGKHN YCRNPDNQRR PWCYVQIGLK QFVQECMVQD 150
CSLSKKPSST VDQQGFQCGQ KALRPRFKIV GGEFTVVENQ PWFAAIYLKN 200
KGGSPPSFKC GGSLISPCWV ASATHCFVNQ PKKEEYVVYL GQSKRNSYNP 250
GEMKFEVEQL ILHEDFSDET LAFHNDIALL KIRTSTGQCA QPSRTIQTIC 300
LPPRFGDAPF GSDCEITGFG QESATDYFYP KDLKMSVVKI ISHEQCKQPH 350
YYGSEINYKM LCAADPEWKT DSCSGDSGGP LICNIDGRPT LSGIVSWGSG 400
CAEKNKPGVY TRVSYFLNWI QSHIGEENGL AF 432
Length:432
Mass (Da):47,957
Last modified:April 1, 1993 - v1
Checksum:i4EB1B96C716244C8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161N → H in CAA46601. 1 Publication
Sequence conflicti24 – 241E → G in CAA46601. 1 Publication
Sequence conflicti332 – 3321D → N in CAA46601. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63434 mRNA. Translation: CAA45028.1.
X65651 mRNA. Translation: CAA46601.1.
X66907 Genomic DNA. Translation: CAA47356.1.
PIRiS24604. S18932.
UniGeneiRn.6064.

Genome annotation databases

UCSCiRGD:3343. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63434 mRNA. Translation: CAA45028.1 .
X65651 mRNA. Translation: CAA46601.1 .
X66907 Genomic DNA. Translation: CAA47356.1 .
PIRi S24604. S18932.
UniGenei Rn.6064.

3D structure databases

ProteinModelPortali P29598.
SMRi P29598. Positions 29-155, 166-427.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1075245.

Protein family/group databases

MEROPSi S01.231.

PTM databases

PhosphoSitei P29598.

Proteomic databases

PaxDbi P29598.
PRIDEi P29598.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:3343. rat.

Organism-specific databases

RGDi 3343. Plau.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000237314.
HOVERGENi HBG008633.
InParanoidi P29598.
PhylomeDBi P29598.

Miscellaneous databases

PROi P29598.

Gene expression databases

Genevestigatori P29598.

Family and domain databases

Gene3Di 2.40.20.10. 1 hit.
InterProi IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00181. EGF. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEi PS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Transcriptional and posttranscriptional activation of urokinase plasminogen activator gene expression in metastatic tumor cells."
    Henderson B.R., Tansey W.P., Phillips S.M., Ramshaw I.A., Kefford R.F.
    Cancer Res. 52:2489-2496(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer 344.
  2. Rabbani S.A.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  3. "The receptor for the plasminogen activator of urokinase type is up-regulated in transformed rat thyroid cells."
    Ragno P., Cassano S., Degen J., Kessler C., Blasi F., Rossi G.
    FEBS Lett. 306:193-198(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-62, IDENTIFICATION OF UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR BINDING DOMAIN.

Entry informationi

Entry nameiUROK_RAT
AccessioniPrimary (citable) accession number: P29598
Secondary accession number(s): Q6LBK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 14, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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