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P29598

- UROK_RAT

UniProt

P29598 - UROK_RAT

Protein

Urokinase-type plasminogen activator

Gene

Plau

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

    Catalytic activityi

    Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

    Enzyme regulationi

    Inhibited by SERPINA5.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei225 – 2251Charge relay system
    Active sitei276 – 2761Charge relay system
    Active sitei377 – 3771Charge relay system

    GO - Molecular functioni

    1. peptidase activity Source: RGD
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. angiogenesis Source: RGD
    2. cellular response to fluid shear stress Source: RGD
    3. cellular response to glucose stimulus Source: RGD
    4. cellular response to hepatocyte growth factor stimulus Source: RGD
    5. cellular response to hypoxia Source: RGD
    6. cellular response to lipopolysaccharide Source: RGD
    7. cellular response to organic substance Source: RGD
    8. cellular response to staurosporine Source: RGD
    9. embryo implantation Source: RGD
    10. neuron death Source: RGD
    11. positive regulation of cell proliferation Source: RGD
    12. positive regulation of ovulation Source: RGD
    13. positive regulation of reactive oxygen species metabolic process Source: RGD
    14. positive regulation of smooth muscle cell migration Source: RGD
    15. regulation of hepatocyte proliferation Source: RGD
    16. response to activity Source: RGD
    17. response to hepatocyte growth factor Source: RGD
    18. response to hyperoxia Source: RGD
    19. skeletal muscle tissue regeneration Source: RGD
    20. spermatogenesis Source: RGD
    21. wound healing Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Plasminogen activation

    Protein family/group databases

    MEROPSiS01.231.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Urokinase-type plasminogen activator (EC:3.4.21.73)
    Short name:
    U-plasminogen activator
    Short name:
    uPA
    Cleaved into the following 3 chains:
    Gene namesi
    Name:Plau
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3343. Plau.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular space Source: RGD
    2. membrane Source: RGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 432413Urokinase-type plasminogen activatorPRO_0000028333Add
    BLAST
    Chaini20 – 177158Urokinase-type plasminogen activator long chain ABy similarityPRO_0000028334Add
    BLAST
    Chaini156 – 17722Urokinase-type plasminogen activator short chain ABy similarityPRO_0000028335Add
    BLAST
    Chaini179 – 432254Urokinase-type plasminogen activator chain BBy similarityPRO_0000028336Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 39By similarity
    Disulfide bondi33 ↔ 51By similarity
    Disulfide bondi53 ↔ 62By similarity
    Disulfide bondi70 ↔ 151By similarity
    Disulfide bondi91 ↔ 133By similarity
    Disulfide bondi122 ↔ 146By similarity
    Modified residuei158 – 1581PhosphoserineBy similarity
    Disulfide bondi168 ↔ 300Interchain (between A and B chains)PROSITE-ProRule annotation
    Disulfide bondi210 ↔ 226By similarity
    Disulfide bondi218 ↔ 289By similarity
    Disulfide bondi314 ↔ 383By similarity
    Disulfide bondi346 ↔ 362By similarity
    Disulfide bondi373 ↔ 401By similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein, Zymogen

    Proteomic databases

    PaxDbiP29598.
    PRIDEiP29598.

    PTM databases

    PhosphoSiteiP29598.

    Expressioni

    Gene expression databases

    GenevestigatoriP29598.

    Interactioni

    Subunit structurei

    Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP29598.
    SMRiP29598. Positions 29-155, 166-427.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 6337EGF-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini70 – 15182KringlePROSITE-ProRule annotationAdd
    BLAST
    Domaini179 – 425247Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni34 – 5724Binds urokinase plasminogen activator surface receptorAdd
    BLAST
    Regioni152 – 17827Connecting peptideAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 kringle domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Kringle, Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000237314.
    HOVERGENiHBG008633.
    InParanoidiP29598.
    PhylomeDBiP29598.

    Family and domain databases

    Gene3Di2.40.20.10. 1 hit.
    InterProiIPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00181. EGF. 1 hit.
    SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 1 hit.
    PROSITEiPS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29598-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVWLASLFL CALVANSEGG SELEASDESN CGCQNGGVCV SYKYFSSIRR    50
    CSCPKKFKGE HCEIDTSKTC YHGNGQSYRG KANTDTKGRP CLAWNSPAVL 100
    QQTYNAHRSD ALSLGLGKHN YCRNPDNQRR PWCYVQIGLK QFVQECMVQD 150
    CSLSKKPSST VDQQGFQCGQ KALRPRFKIV GGEFTVVENQ PWFAAIYLKN 200
    KGGSPPSFKC GGSLISPCWV ASATHCFVNQ PKKEEYVVYL GQSKRNSYNP 250
    GEMKFEVEQL ILHEDFSDET LAFHNDIALL KIRTSTGQCA QPSRTIQTIC 300
    LPPRFGDAPF GSDCEITGFG QESATDYFYP KDLKMSVVKI ISHEQCKQPH 350
    YYGSEINYKM LCAADPEWKT DSCSGDSGGP LICNIDGRPT LSGIVSWGSG 400
    CAEKNKPGVY TRVSYFLNWI QSHIGEENGL AF 432
    Length:432
    Mass (Da):47,957
    Last modified:April 1, 1993 - v1
    Checksum:i4EB1B96C716244C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161N → H in CAA46601. 1 PublicationCurated
    Sequence conflicti24 – 241E → G in CAA46601. 1 PublicationCurated
    Sequence conflicti332 – 3321D → N in CAA46601. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63434 mRNA. Translation: CAA45028.1.
    X65651 mRNA. Translation: CAA46601.1.
    X66907 Genomic DNA. Translation: CAA47356.1.
    PIRiS24604. S18932.
    UniGeneiRn.6064.

    Genome annotation databases

    UCSCiRGD:3343. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63434 mRNA. Translation: CAA45028.1 .
    X65651 mRNA. Translation: CAA46601.1 .
    X66907 Genomic DNA. Translation: CAA47356.1 .
    PIRi S24604. S18932.
    UniGenei Rn.6064.

    3D structure databases

    ProteinModelPortali P29598.
    SMRi P29598. Positions 29-155, 166-427.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1075245.

    Protein family/group databases

    MEROPSi S01.231.

    PTM databases

    PhosphoSitei P29598.

    Proteomic databases

    PaxDbi P29598.
    PRIDEi P29598.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:3343. rat.

    Organism-specific databases

    RGDi 3343. Plau.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000237314.
    HOVERGENi HBG008633.
    InParanoidi P29598.
    PhylomeDBi P29598.

    Miscellaneous databases

    PROi P29598.

    Gene expression databases

    Genevestigatori P29598.

    Family and domain databases

    Gene3Di 2.40.20.10. 1 hit.
    InterProi IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00181. EGF. 1 hit.
    SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 1 hit.
    PROSITEi PS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcriptional and posttranscriptional activation of urokinase plasminogen activator gene expression in metastatic tumor cells."
      Henderson B.R., Tansey W.P., Phillips S.M., Ramshaw I.A., Kefford R.F.
      Cancer Res. 52:2489-2496(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Fischer 344.
    2. Rabbani S.A.
      Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    3. "The receptor for the plasminogen activator of urokinase type is up-regulated in transformed rat thyroid cells."
      Ragno P., Cassano S., Degen J., Kessler C., Blasi F., Rossi G.
      FEBS Lett. 306:193-198(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-62, IDENTIFICATION OF UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR BINDING DOMAIN.

    Entry informationi

    Entry nameiUROK_RAT
    AccessioniPrimary (citable) accession number: P29598
    Secondary accession number(s): Q6LBK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3