P29595 (NEDD8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NEDD8

Alternative name(s):
Neddylin
Neural precursor cell expressed developmentally down-regulated protein 8
Short name=NEDD-8
Ubiquitin-like protein Nedd8

Gene names

Name:	Nedd8
Synonyms:	Nedd-8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	81 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Ref.6
Subunit structure	Directly interacts with NUB1. Covalently attached to cullins and p53 By similarity. Interacts with AHR. Ref.7
Subcellular location	Nucleus. Note: Mainly nuclear. Ref.7
Tissue specificity	Ubiquitously expressed (at protein level). Ref.2 Ref.8
Developmental stage	According to Ref.1 and Ref.8, down-regulated during embryonic development, but according to Ref.5, expression does not change during embryonic development. Between E10 and E12, expressed in heart and spinal ganglia. Between E13.5 and E16.5, strongly expressed in spinal and sympathetic ganglia, neural epithelium of the retina, oral and olfactory epithelia and thymus. Ref.4 Ref.5 Ref.7 Ref.8
Post-translational modification	Cleavage of precursor form by UCHL3 or SENP8 is necessary for function By similarity.
Sequence similarities	Belongs to the ubiquitin family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
PTM	Isopeptide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein localization Inferred from direct assay Ref.7. Source: MGI protein neddylation Inferred from sequence alignment Ref.4. Source: MGI regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.7. Source: MGI response to organic cyclic compound Inferred from electronic annotation. Source: Compara
Cellular_component	nucleus Inferred from direct assay Ref.7. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 76	76	NEDD8		PRO_0000042769
	Propeptide	77 – 81	5	By similarity		PRO_0000042770
Regions
	Region	70 – 72	3	Interaction with UBE1C By similarity
Sites
	Site	8	1	Interaction with UBE1C By similarity
	Site	44	1	Interaction with UBE1C By similarity
Amino acid modifications
	Cross-link	76		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Sequences

	Sequence		Length	Mass (Da)
	P29595 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: DC339102BE4725D2	FASTA	81	8,972
	10 20 30 40 50 60 MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM NDEKTAADYK 70 80 ILGGSVLHLV LALRGGGGLG Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a set of genes with developmentally down-regulated expression in the mouse brain." Kumar S., Tomooka Y., Noda M. Biochem. Biophys. Res. Commun. 185:1155-1161(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Cloning of a cDNA which encodes a novel ubiquitin-like protein." Kumar S., Yoshida Y., Noda M. Biochem. Biophys. Res. Commun. 195:393-399(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SEQUENCE REVISION, TISSUE SPECIFICITY. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[4]	"Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein." Kamitani T., Kito K., Nguyen H.P., Yeh E.T.H. J. Biol. Chem. 272:28557-28562(1997) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[5]	"Covalent modification of all members of human cullin family proteins by NEDD8." Hori T., Osaka F., Chiba T., Miyamoto C., Okabayashi K., Shimbara N., Kato S., Tanaka K. Oncogene 18:6829-6834(1999) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[6]	"The NEDD8 system is essential for cell cycle progression and morphogenetic pathway in mice." Tateishi K., Omata M., Tanaka K., Chiba T. J. Cell Biol. 155:571-580(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Interaction with Nedd8, a ubiquitin-like protein, enhances the transcriptional activity of the aryl hydrocarbon receptor." Antenos M., Casper R.F., Brown T.J. J. Biol. Chem. 277:44028-44034(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AHR, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[8]	"Expression pattern of the JAB1/CSN5 gene during murine embryogenesis: colocalization with NEDD8." Carrabino S., Carminati E., Talarico D., Pardi R., Bianchi E. Gene Expr. Patterns 4:423-431(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10918 mRNA. Translation: BAA01719.1. BC004625 mRNA. Translation: AAH04625.1.
IPI	IPI00127021.
PIR	JN0710.
RefSeq	NP_032709.1. NM_008683.3.
UniGene	Mm.296566.
3D structure databases
ProteinModelPortal	P29595.
SMR	P29595. Positions 1-76.
ModBase	Search...
PTM databases
PhosphoSite	P29595.
Proteomic databases
PaxDb	P29595.
PRIDE	P29595.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000010520; ENSMUSP00000010520; ENSMUSG00000010376. ENSMUST00000163750; ENSMUSP00000130492; ENSMUSG00000010376.
GeneID	18002.
KEGG	mmu:18002.
Organism-specific databases
CTD	4738.
MGI	MGI:97301. Nedd8.
Phylogenomic databases
eggNOG	COG5272.
HOGENOM	HOG000233942.
HOVERGEN	HBG000057.
InParanoid	P29595.
KO	K12158.
OMA	EPTDKVD.
OrthoDB	EOG4N04GQ.
Gene expression databases
ArrayExpress	P29595.
Bgee	P29595.
Genevestigator	P29595.
GermOnline	ENSMUSG00000010376. Mus musculus.
Family and domain databases
InterPro	IPR000626. Ubiquitin. IPR019954. Ubiquitin_CS. IPR019956. Ubiquitin_subgr. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF00240. ubiquitin. 1 hit. [Graphical view]
PRINTS	PR00348. UBIQUITIN.
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS00299. UBIQUITIN_1. 1 hit. PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	293003.
SOURCE	Search...

Entry information

Entry name

NEDD8_MOUSE

Accession

Primary (citable) accession number: P29595

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program