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P29595

- NEDD8_MOUSE

UniProt

P29595 - NEDD8_MOUSE

Protein

NEDD8

Gene

Nedd8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei8 – 81Interaction with UBE1CBy similarity
    Sitei44 – 441Interaction with UBE1CBy similarity

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. protein localization Source: MGI
    2. protein neddylation Source: MGI
    3. regulation of transcription from RNA polymerase II promoter Source: MGI
    4. response to organic cyclic compound Source: Ensembl

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_203510. TGF-beta receptor signaling activates SMADs.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NEDD8
    Alternative name(s):
    Neddylin
    Neural precursor cell expressed developmentally down-regulated protein 8
    Short name:
    NEDD-8
    Ubiquitin-like protein Nedd8
    Gene namesi
    Name:Nedd8
    Synonyms:Nedd-8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:97301. Nedd8.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Mainly nuclear.

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7676NEDD8PRO_0000042769Add
    BLAST
    Propeptidei77 – 815By similarityPRO_0000042770

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei48 – 481N6-acetyllysine1 Publication
    Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

    Post-translational modificationi

    Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond

    Proteomic databases

    MaxQBiP29595.
    PaxDbiP29595.
    PRIDEiP29595.

    PTM databases

    PhosphoSiteiP29595.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed (at protein level).2 Publications

    Developmental stagei

    According to PubMed:1378265 and PubMed:15183309, down-regulated during embryonic development, but according to PubMed:10597293, expression does not change during embryonic development. Between E10 and E12, expressed in heart and spinal ganglia. Between E13.5 and E16.5, strongly expressed in spinal and sympathetic ganglia, neural epithelium of the retina, oral and olfactory epithelia and thymus.4 Publications

    Gene expression databases

    ArrayExpressiP29595.
    BgeeiP29595.
    GenevestigatoriP29595.

    Interactioni

    Subunit structurei

    Directly interacts with NUB1. Covalently attached to cullins and p53 By similarity. Interacts with AHR.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi201725. 9 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP29595.
    SMRiP29595. Positions 1-76.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni70 – 723Interaction with UBE1CBy similarity

    Sequence similaritiesi

    Belongs to the ubiquitin family.Curated

    Phylogenomic databases

    eggNOGiCOG5272.
    HOGENOMiHOG000233942.
    HOVERGENiHBG000057.
    InParanoidiP29595.
    KOiK12158.
    OMAiGKQMTDD.
    OrthoDBiEOG7JDR1W.
    PhylomeDBiP29595.
    TreeFamiTF300072.

    Family and domain databases

    InterProiIPR019956. Ubiquitin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view]
    PfamiPF00240. ubiquitin. 1 hit.
    [Graphical view]
    PRINTSiPR00348. UBIQUITIN.
    SMARTiSM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29595-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM   50
    NDEKTAADYK ILGGSVLHLV LALRGGGGLG Q 81
    Length:81
    Mass (Da):8,972
    Last modified:November 1, 1995 - v2
    Checksum:iDC339102BE4725D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10918 mRNA. Translation: BAA01719.1.
    BC004625 mRNA. Translation: AAH04625.1.
    CCDSiCCDS49498.1.
    PIRiJN0710.
    RefSeqiNP_032709.1. NM_008683.3.
    UniGeneiMm.296566.

    Genome annotation databases

    EnsembliENSMUST00000010520; ENSMUSP00000010520; ENSMUSG00000010376.
    ENSMUST00000163750; ENSMUSP00000130492; ENSMUSG00000010376.
    GeneIDi18002.
    KEGGimmu:18002.
    UCSCiuc007uac.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10918 mRNA. Translation: BAA01719.1 .
    BC004625 mRNA. Translation: AAH04625.1 .
    CCDSi CCDS49498.1.
    PIRi JN0710.
    RefSeqi NP_032709.1. NM_008683.3.
    UniGenei Mm.296566.

    3D structure databases

    ProteinModelPortali P29595.
    SMRi P29595. Positions 1-76.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201725. 9 interactions.

    PTM databases

    PhosphoSitei P29595.

    Proteomic databases

    MaxQBi P29595.
    PaxDbi P29595.
    PRIDEi P29595.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010520 ; ENSMUSP00000010520 ; ENSMUSG00000010376 .
    ENSMUST00000163750 ; ENSMUSP00000130492 ; ENSMUSG00000010376 .
    GeneIDi 18002.
    KEGGi mmu:18002.
    UCSCi uc007uac.1. mouse.

    Organism-specific databases

    CTDi 4738.
    MGIi MGI:97301. Nedd8.

    Phylogenomic databases

    eggNOGi COG5272.
    HOGENOMi HOG000233942.
    HOVERGENi HBG000057.
    InParanoidi P29595.
    KOi K12158.
    OMAi GKQMTDD.
    OrthoDBi EOG7JDR1W.
    PhylomeDBi P29595.
    TreeFami TF300072.

    Enzyme and pathway databases

    Reactomei REACT_203510. TGF-beta receptor signaling activates SMADs.

    Miscellaneous databases

    NextBioi 293003.
    PROi P29595.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P29595.
    Bgeei P29595.
    Genevestigatori P29595.

    Family and domain databases

    InterProi IPR019956. Ubiquitin.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    [Graphical view ]
    Pfami PF00240. ubiquitin. 1 hit.
    [Graphical view ]
    PRINTSi PR00348. UBIQUITIN.
    SMARTi SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a set of genes with developmentally down-regulated expression in the mouse brain."
      Kumar S., Tomooka Y., Noda M.
      Biochem. Biophys. Res. Commun. 185:1155-1161(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Cloning of a cDNA which encodes a novel ubiquitin-like protein."
      Kumar S., Yoshida Y., Noda M.
      Biochem. Biophys. Res. Commun. 195:393-399(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SEQUENCE REVISION, TISSUE SPECIFICITY.
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein."
      Kamitani T., Kito K., Nguyen H.P., Yeh E.T.H.
      J. Biol. Chem. 272:28557-28562(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "Covalent modification of all members of human cullin family proteins by NEDD8."
      Hori T., Osaka F., Chiba T., Miyamoto C., Okabayashi K., Shimbara N., Kato S., Tanaka K.
      Oncogene 18:6829-6834(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    6. "The NEDD8 system is essential for cell cycle progression and morphogenetic pathway in mice."
      Tateishi K., Omata M., Tanaka K., Chiba T.
      J. Cell Biol. 155:571-580(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Interaction with Nedd8, a ubiquitin-like protein, enhances the transcriptional activity of the aryl hydrocarbon receptor."
      Antenos M., Casper R.F., Brown T.J.
      J. Biol. Chem. 277:44028-44034(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AHR, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    8. "Expression pattern of the JAB1/CSN5 gene during murine embryogenesis: colocalization with NEDD8."
      Carrabino S., Carminati E., Talarico D., Pardi R., Bianchi E.
      Gene Expr. Patterns 4:423-431(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiNEDD8_MOUSE
    AccessioniPrimary (citable) accession number: P29595
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3