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Protein

NEDD8

Gene

Nedd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei8 – 81Interaction with UBE1CBy similarity
Sitei44 – 441Interaction with UBE1CBy similarity

GO - Molecular functioni

GO - Biological processi

  • protein localization Source: MGI
  • protein neddylation Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to organic cyclic compound Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_297798. TGF-beta receptor signaling activates SMADs.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8
Alternative name(s):
Neddylin
Neural precursor cell expressed developmentally down-regulated protein 8
Short name:
NEDD-8
Ubiquitin-like protein Nedd8
Gene namesi
Name:Nedd8
Synonyms:Nedd-8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:97301. Nedd8.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Mainly nuclear.

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7676NEDD8PRO_0000042769Add
BLAST
Propeptidei77 – 815By similarityPRO_0000042770

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481N6-acetyllysine1 Publication
Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Post-translational modificationi

Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond

Proteomic databases

MaxQBiP29595.
PaxDbiP29595.
PRIDEiP29595.

PTM databases

PhosphoSiteiP29595.

Expressioni

Tissue specificityi

Ubiquitously expressed (at protein level).2 Publications

Developmental stagei

According to PubMed:1378265 and PubMed:15183309, down-regulated during embryonic development, but according to PubMed:10597293, expression does not change during embryonic development. Between E10 and E12, expressed in heart and spinal ganglia. Between E13.5 and E16.5, strongly expressed in spinal and sympathetic ganglia, neural epithelium of the retina, oral and olfactory epithelia and thymus.4 Publications

Gene expression databases

BgeeiP29595.
ExpressionAtlasiP29595. baseline and differential.
GenevisibleiP29595. MM.

Interactioni

Subunit structurei

Directly interacts with NUB1. Covalently attached to cullins and p53 (By similarity). Interacts with AHR.By similarity1 Publication

Protein-protein interaction databases

BioGridi201725. 9 interactions.
STRINGi10090.ENSMUSP00000010520.

Structurei

3D structure databases

ProteinModelPortaliP29595.
SMRiP29595. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 723Interaction with UBE1CBy similarity

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Phylogenomic databases

eggNOGiCOG5272.
HOGENOMiHOG000233942.
HOVERGENiHBG000057.
InParanoidiP29595.
KOiK12158.
OMAiEPTDKVD.
OrthoDBiEOG7JDR1W.
PhylomeDBiP29595.
TreeFamiTF300072.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM
60 70 80
NDEKTAADYK ILGGSVLHLV LALRGGGGLG Q
Length:81
Mass (Da):8,972
Last modified:November 1, 1995 - v2
Checksum:iDC339102BE4725D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10918 mRNA. Translation: BAA01719.1.
BC004625 mRNA. Translation: AAH04625.1.
CCDSiCCDS49498.1.
PIRiJN0710.
RefSeqiNP_032709.1. NM_008683.3.
UniGeneiMm.296566.

Genome annotation databases

EnsembliENSMUST00000010520; ENSMUSP00000010520; ENSMUSG00000010376.
ENSMUST00000163750; ENSMUSP00000130492; ENSMUSG00000010376.
GeneIDi18002.
KEGGimmu:18002.
UCSCiuc007uac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10918 mRNA. Translation: BAA01719.1.
BC004625 mRNA. Translation: AAH04625.1.
CCDSiCCDS49498.1.
PIRiJN0710.
RefSeqiNP_032709.1. NM_008683.3.
UniGeneiMm.296566.

3D structure databases

ProteinModelPortaliP29595.
SMRiP29595. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201725. 9 interactions.
STRINGi10090.ENSMUSP00000010520.

PTM databases

PhosphoSiteiP29595.

Proteomic databases

MaxQBiP29595.
PaxDbiP29595.
PRIDEiP29595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010520; ENSMUSP00000010520; ENSMUSG00000010376.
ENSMUST00000163750; ENSMUSP00000130492; ENSMUSG00000010376.
GeneIDi18002.
KEGGimmu:18002.
UCSCiuc007uac.1. mouse.

Organism-specific databases

CTDi4738.
MGIiMGI:97301. Nedd8.

Phylogenomic databases

eggNOGiCOG5272.
HOGENOMiHOG000233942.
HOVERGENiHBG000057.
InParanoidiP29595.
KOiK12158.
OMAiEPTDKVD.
OrthoDBiEOG7JDR1W.
PhylomeDBiP29595.
TreeFamiTF300072.

Enzyme and pathway databases

ReactomeiREACT_297798. TGF-beta receptor signaling activates SMADs.

Miscellaneous databases

NextBioi293003.
PROiP29595.
SOURCEiSearch...

Gene expression databases

BgeeiP29595.
ExpressionAtlasiP29595. baseline and differential.
GenevisibleiP29595. MM.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a set of genes with developmentally down-regulated expression in the mouse brain."
    Kumar S., Tomooka Y., Noda M.
    Biochem. Biophys. Res. Commun. 185:1155-1161(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Cloning of a cDNA which encodes a novel ubiquitin-like protein."
    Kumar S., Yoshida Y., Noda M.
    Biochem. Biophys. Res. Commun. 195:393-399(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SEQUENCE REVISION, TISSUE SPECIFICITY.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein."
    Kamitani T., Kito K., Nguyen H.P., Yeh E.T.H.
    J. Biol. Chem. 272:28557-28562(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "Covalent modification of all members of human cullin family proteins by NEDD8."
    Hori T., Osaka F., Chiba T., Miyamoto C., Okabayashi K., Shimbara N., Kato S., Tanaka K.
    Oncogene 18:6829-6834(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "The NEDD8 system is essential for cell cycle progression and morphogenetic pathway in mice."
    Tateishi K., Omata M., Tanaka K., Chiba T.
    J. Cell Biol. 155:571-580(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Interaction with Nedd8, a ubiquitin-like protein, enhances the transcriptional activity of the aryl hydrocarbon receptor."
    Antenos M., Casper R.F., Brown T.J.
    J. Biol. Chem. 277:44028-44034(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AHR, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  8. "Expression pattern of the JAB1/CSN5 gene during murine embryogenesis: colocalization with NEDD8."
    Carrabino S., Carminati E., Talarico D., Pardi R., Bianchi E.
    Gene Expr. Patterns 4:423-431(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNEDD8_MOUSE
AccessioniPrimary (citable) accession number: P29595
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.