Reviewed, UniProtKB/Swiss-Prot P29568 (MTHZ_METTF)

Names and origin

Protein names

Recommended name:
    Modification methylase MthZI
      Short name=M.MthZI
    EC=2.1.1.113
Alternative name(s):
    N-4 cytosine-specific methyltransferase MthZI

Gene names

Name:

mthZIM

Encoded on

Plasmid pFZ1

Organism

Methanobacterium thermoformicicum

Taxonomic identifier

145262 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CTAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MthZI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Modification methylase MthZI		PRO_0000087928

Sequences

	Sequence		Length	Mass (Da)
	P29568-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 0FC26598445CA704	FASTA	355	42,477
	10 20 30 40 50 60 MKTTHRIYFK NSADMNELKD KSINLVVTSP PYPMVEIWDR LFSELNPKIE ETLIDEEDGL 70 80 90 100 110 120 RSYNLMHEEL EKVWHEVDRV TAPGGVVIIN IGDATRKIGK KFQLYPNHVR TIDFFFDRGY 130 140 150 160 170 180 QVLPFIIWRK QSNKPTKFMG SGMLPPNAYV THEHEYILIF RKEGPRQFKT EEERKLRRES 190 200 210 220 230 240 AYFWEERNQW FSDVWTDLTG VSQRLNHKNL RKRAAAYPFE LAYRLINMYS IMGDWVLDPF 250 260 270 280 290 300 LGTGTTMIAA ACAGRNSIGY ELDHNFKDLI ESRINETLKL SNEIVMRRIN DHIEFIREKN 310 320 330 340 350 GKYYSENYKF KVTTRQEQDI RLYYPRTYKK IKNNEFEFFY QEVNPKKERQ SKLNI

References

[1]	"Identification of the CTAG-recognizing restriction-modification systems MthZI and MthFI from Methanobacterium thermoformicicum and characterization of the plasmid-encoded mthZIM gene." Noelling J., de Vos W.M. Nucleic Acids Res. 20:5047-5052(1992) [PubMed: 1408820] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3720 / Z-245.
[2]	"Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum." Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M. Nucleic Acids Res. 20:6501-6507(1992) [PubMed: 1336177] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3720 / Z-245.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67212 Genomic DNA. Translation: CAA47651.1. X68367 Genomic DNA. Translation: CAA48447.1.
PIR	S30315.
RefSeq	NP_039775.1.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3540. M.MthZI.
Genome annotation databases
GeneID	2193587.
Enzyme and pathway databases
BRENDA	2.1.1.113. 258835.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTHZ_METTF

Accession

Primary (citable) accession number: P29568

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)