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P29567

- MTHT_METTF

UniProt

P29567 - MTHT_METTF

Protein

Modification methylase MthTI

Gene

mthTIM

Organism
Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the MthTI endonuclease.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3448. M.MthTI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase MthTI (EC:2.1.1.37)
    Short name:
    M.MthTI
    Alternative name(s):
    Cytosine-specific methyltransferase MthTI
    Gene namesi
    Name:mthTIM
    Encoded oniPlasmid pFV10 Publication
    OrganismiMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
    Taxonomic identifieri145262 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 330330Modification methylase MthTIPRO_0000087894Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP29567.
    SMRiP29567. Positions 3-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 328326SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29567-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNMDIASFFS GAGGLDLGFT KAGFNIVFAN DNWKGCWKTF EKNHGIKINK    50
    KPIEWLKPSE IPDVVGFIGG PPCQSWSLAG SMCGADDPRG KTFYAYVDLV 100
    KEKDPLFFLA ENVPGIVSRT HLPEFKRLVN SFIDIGYNVE YKVLNAKDYG 150
    VPQDRKRVFI VGYREDLNLK FEFPKPLNKK VTLRDAIGDL PEPKPALEKN 200
    RSNGENLEVP NHEYMTGTFS SRYMSRNRVR SWDEVSFTIQ AGGRHAPCHP 250
    QANKMIKVGP DKFIFDPESP KPYRRLSVRE CARIQGFPDD FIFYYKNVAD 300
    GYTMVGNAVP VKLAEELAKK IKKDLEGVLN 330
    Length:330
    Mass (Da):37,360
    Last modified:April 1, 1993 - v1
    Checksum:iEFC15E57D86385A5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97222 Genomic DNA. Translation: AAA73370.1.
    X68366 Genomic DNA. Translation: CAA48436.1.
    PIRiB42941.
    RefSeqiNP_039765.1. NC_001336.1.
    WP_010889851.1. NC_001336.1.

    Genome annotation databases

    GeneIDi2193586.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97222 Genomic DNA. Translation: AAA73370.1 .
    X68366 Genomic DNA. Translation: CAA48436.1 .
    PIRi B42941.
    RefSeqi NP_039765.1. NC_001336.1.
    WP_010889851.1. NC_001336.1.

    3D structure databases

    ProteinModelPortali P29567.
    SMRi P29567. Positions 3-325.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3448. M.MthTI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2193586.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae."
      Noelling J., de Vos W.M.
      J. Bacteriol. 174:5719-5726(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY.
      Strain: DSM 3848 / THF.
    2. "Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum."
      Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.
      Nucleic Acids Res. 20:6501-6507(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 3848 / THF.

    Entry informationi

    Entry nameiMTHT_METTF
    AccessioniPrimary (citable) accession number: P29567
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3