Reviewed, UniProtKB/Swiss-Prot P29567 (MTHT_METTF)

Names and origin

Protein names

Recommended name:
    Modification methylase MthTI
      Short name=M.MthTI
    EC=2.1.1.37
Alternative name(s):
    Cytosine-specific methyltransferase MthTI

Gene names

Name:

mthTIM

Encoded on

Plasmid pFV1

Organism

Methanobacterium thermoformicicum

Taxonomic identifier

145262 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the MthTI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Modification methylase MthTI		PRO_0000087894
Sites
	Active site	73	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29567-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: EFC15E57D86385A5	FASTA	330	37,360
	10 20 30 40 50 60 MNMDIASFFS GAGGLDLGFT KAGFNIVFAN DNWKGCWKTF EKNHGIKINK KPIEWLKPSE 70 80 90 100 110 120 IPDVVGFIGG PPCQSWSLAG SMCGADDPRG KTFYAYVDLV KEKDPLFFLA ENVPGIVSRT 130 140 150 160 170 180 HLPEFKRLVN SFIDIGYNVE YKVLNAKDYG VPQDRKRVFI VGYREDLNLK FEFPKPLNKK 190 200 210 220 230 240 VTLRDAIGDL PEPKPALEKN RSNGENLEVP NHEYMTGTFS SRYMSRNRVR SWDEVSFTIQ 250 260 270 280 290 300 AGGRHAPCHP QANKMIKVGP DKFIFDPESP KPYRRLSVRE CARIQGFPDD FIFYYKNVAD 310 320 330 GYTMVGNAVP VKLAEELAKK IKKDLEGVLN

References

[1]	"Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae." Noelling J., de Vos W.M. J. Bacteriol. 174:5719-5726(1992) [PubMed: 1512204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3848 / THF.
[2]	"Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum." Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M. Nucleic Acids Res. 20:6501-6507(1992) [PubMed: 1336177] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3848 / THF.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M97222 Genomic DNA. Translation: AAA73370.1. X68366 Genomic DNA. Translation: CAA48436.1.
PIR	B42941.
RefSeq	NP_039765.1.
3D structure databases
HSSP	HSSP built from PDB template 1DCT based on UniProtKB P20589.
ModBase	Search...
Protein family/group databases
REBASE	3448. M.MthTI.
Genome annotation databases
GeneID	2193586.
Enzyme and pathway databases
BRENDA	2.1.1.37. 258835.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. False negative. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTHT_METTF

Accession

Primary (citable) accession number: P29567

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	March 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)