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P29567

- MTHT_METTF

UniProt

P29567 - MTHT_METTF

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Protein

Modification methylase MthTI

Gene
mthTIM
Organism
Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the MthTI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3448. M.MthTI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase MthTI (EC:2.1.1.37)
Short name:
M.MthTI
Alternative name(s):
Cytosine-specific methyltransferase MthTI
Gene namesi
Name:mthTIM
Encoded oniPlasmid pFV10 Publication
OrganismiMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Taxonomic identifieri145262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Modification methylase MthTIPRO_0000087894Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP29567.
SMRiP29567. Positions 3-325.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 328326SAM-dependent MTase C5-typeAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29567-1 [UniParc]FASTAAdd to Basket

« Hide

MNMDIASFFS GAGGLDLGFT KAGFNIVFAN DNWKGCWKTF EKNHGIKINK    50
KPIEWLKPSE IPDVVGFIGG PPCQSWSLAG SMCGADDPRG KTFYAYVDLV 100
KEKDPLFFLA ENVPGIVSRT HLPEFKRLVN SFIDIGYNVE YKVLNAKDYG 150
VPQDRKRVFI VGYREDLNLK FEFPKPLNKK VTLRDAIGDL PEPKPALEKN 200
RSNGENLEVP NHEYMTGTFS SRYMSRNRVR SWDEVSFTIQ AGGRHAPCHP 250
QANKMIKVGP DKFIFDPESP KPYRRLSVRE CARIQGFPDD FIFYYKNVAD 300
GYTMVGNAVP VKLAEELAKK IKKDLEGVLN 330
Length:330
Mass (Da):37,360
Last modified:April 1, 1993 - v1
Checksum:iEFC15E57D86385A5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97222 Genomic DNA. Translation: AAA73370.1.
X68366 Genomic DNA. Translation: CAA48436.1.
PIRiB42941.
RefSeqiNP_039765.1. NC_001336.1.
WP_010889851.1. NC_001336.1.

Genome annotation databases

GeneIDi2193586.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97222 Genomic DNA. Translation: AAA73370.1 .
X68366 Genomic DNA. Translation: CAA48436.1 .
PIRi B42941.
RefSeqi NP_039765.1. NC_001336.1.
WP_010889851.1. NC_001336.1.

3D structure databases

ProteinModelPortali P29567.
SMRi P29567. Positions 3-325.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3448. M.MthTI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2193586.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae."
    Noelling J., de Vos W.M.
    J. Bacteriol. 174:5719-5726(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 3848 / THF.
  2. "Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum."
    Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.
    Nucleic Acids Res. 20:6501-6507(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 3848 / THF.

Entry informationi

Entry nameiMTHT_METTF
AccessioniPrimary (citable) accession number: P29567
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 3, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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