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Protein

Modification methylase MthTI

Gene

mthTIM

Organism
Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the MthTI endonuclease.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3448. M.MthTI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase MthTI (EC:2.1.1.37)
Short name:
M.MthTI
Alternative name(s):
Cytosine-specific methyltransferase MthTI
Gene namesi
Name:mthTIM
Encoded oniPlasmid pFV10 Publication
OrganismiMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Taxonomic identifieri145262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Modification methylase MthTIPRO_0000087894Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP29567.
SMRiP29567. Positions 3-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 328326SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29567-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMDIASFFS GAGGLDLGFT KAGFNIVFAN DNWKGCWKTF EKNHGIKINK
60 70 80 90 100
KPIEWLKPSE IPDVVGFIGG PPCQSWSLAG SMCGADDPRG KTFYAYVDLV
110 120 130 140 150
KEKDPLFFLA ENVPGIVSRT HLPEFKRLVN SFIDIGYNVE YKVLNAKDYG
160 170 180 190 200
VPQDRKRVFI VGYREDLNLK FEFPKPLNKK VTLRDAIGDL PEPKPALEKN
210 220 230 240 250
RSNGENLEVP NHEYMTGTFS SRYMSRNRVR SWDEVSFTIQ AGGRHAPCHP
260 270 280 290 300
QANKMIKVGP DKFIFDPESP KPYRRLSVRE CARIQGFPDD FIFYYKNVAD
310 320 330
GYTMVGNAVP VKLAEELAKK IKKDLEGVLN
Length:330
Mass (Da):37,360
Last modified:April 1, 1993 - v1
Checksum:iEFC15E57D86385A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97222 Genomic DNA. Translation: AAA73370.1.
X68366 Genomic DNA. Translation: CAA48436.1.
PIRiB42941.
RefSeqiNP_039765.1. NC_001336.1.
WP_010889851.1. NC_001336.1.

Genome annotation databases

GeneIDi2193586.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97222 Genomic DNA. Translation: AAA73370.1.
X68366 Genomic DNA. Translation: CAA48436.1.
PIRiB42941.
RefSeqiNP_039765.1. NC_001336.1.
WP_010889851.1. NC_001336.1.

3D structure databases

ProteinModelPortaliP29567.
SMRiP29567. Positions 3-325.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3448. M.MthTI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2193586.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae."
    Noelling J., de Vos W.M.
    J. Bacteriol. 174:5719-5726(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY.
    Strain: DSM 3848 / THF.
  2. "Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum."
    Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.
    Nucleic Acids Res. 20:6501-6507(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 3848 / THF.

Entry informationi

Entry nameiMTHT_METTF
AccessioniPrimary (citable) accession number: P29567
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.