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P29567 (MTHT_METTF) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase MthTI

Short name=M.MthTI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase MthTI
Gene names
Name:mthTIM
Encoded onPlasmid pFV1
OrganismMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Taxonomic identifier145262 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the MthTI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330Modification methylase MthTI
PRO_0000087894

Regions

Domain3 – 328326SAM-dependent MTase C5-type

Sites

Active site731 By similarity

Sequences

Sequence LengthMass (Da)Tools
P29567 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: EFC15E57D86385A5

FASTA33037,360
        10         20         30         40         50         60 
MNMDIASFFS GAGGLDLGFT KAGFNIVFAN DNWKGCWKTF EKNHGIKINK KPIEWLKPSE 

        70         80         90        100        110        120 
IPDVVGFIGG PPCQSWSLAG SMCGADDPRG KTFYAYVDLV KEKDPLFFLA ENVPGIVSRT 

       130        140        150        160        170        180 
HLPEFKRLVN SFIDIGYNVE YKVLNAKDYG VPQDRKRVFI VGYREDLNLK FEFPKPLNKK 

       190        200        210        220        230        240 
VTLRDAIGDL PEPKPALEKN RSNGENLEVP NHEYMTGTFS SRYMSRNRVR SWDEVSFTIQ 

       250        260        270        280        290        300 
AGGRHAPCHP QANKMIKVGP DKFIFDPESP KPYRRLSVRE CARIQGFPDD FIFYYKNVAD 

       310        320        330 
GYTMVGNAVP VKLAEELAKK IKKDLEGVLN 

« Hide

References

[1]"Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae."
Noelling J., de Vos W.M.
J. Bacteriol. 174:5719-5726(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 3848 / THF.
[2]"Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum."
Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.
Nucleic Acids Res. 20:6501-6507(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 3848 / THF.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M97222 Genomic DNA. Translation: AAA73370.1.
X68366 Genomic DNA. Translation: CAA48436.1.
PIRB42941.
RefSeqNP_039765.1. NC_001336.1.

3D structure databases

ProteinModelPortalP29567.
SMRP29567. Positions 3-325.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3448. M.MthTI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2193586.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTHT_METTF
AccessionPrimary (citable) accession number: P29567
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries