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P29566

- T2MZ_METTF

UniProt

P29566 - T2MZ_METTF

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Protein

Probable type-2 restriction enzyme MthZI

Gene
mthZIR
Organism
Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Status
Reviewed - Annotation score: 2 out of 5 - Protein predictedi

Functioni

Recognizes the double-stranded sequence CTAG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi2204. MthZI.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable type-2 restriction enzyme MthZI (EC:3.1.21.4)
Short name:
R.MthZI
Alternative name(s):
Endonuclease MthZI
Type II restriction enzyme MthZI
Gene namesi
Name:mthZIR
Encoded oniPlasmid pFZ10 Publication
OrganismiMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Taxonomic identifieri145262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Probable type-2 restriction enzyme MthZIPRO_0000077340Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019068. Restrct_endonuc_II_MjaI.
[Graphical view]
PfamiPF09568. RE_MjaI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29566-1 [UniParc]FASTAAdd to Basket

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MIDNFKEIRL DFKDELKKIT GKEVEFPKYT TQIINLANQN AQGTRPRVVG    50
QMSDLIHECP DKSYEGWKKW YLEHYSDRIE KATKKISKMI ENMKAAMELI 100
DEEMIRKWVE DLVITKTAEG LIIQEIILKT IAEEAGLEWR LATSKEESKN 150
IDGFIGSTPV SIKPMSYESM RPTVREEIDI QTIFYKKPKN SRYLYIYHNL 200
NI 202
Length:202
Mass (Da):23,742
Last modified:April 1, 1993 - v1
Checksum:i1AC0274DEC882F49
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67212 Genomic DNA. Translation: CAA47652.1.
X68367 Genomic DNA. Translation: CAA48446.1.
PIRiS26074.
RefSeqiNP_039774.1. NC_001337.1.
WP_010889861.1. NC_001337.1.

Genome annotation databases

GeneIDi3355220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67212 Genomic DNA. Translation: CAA47652.1 .
X68367 Genomic DNA. Translation: CAA48446.1 .
PIRi S26074.
RefSeqi NP_039774.1. NC_001337.1.
WP_010889861.1. NC_001337.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 2204. MthZI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3355220.

Family and domain databases

InterProi IPR019068. Restrct_endonuc_II_MjaI.
[Graphical view ]
Pfami PF09568. RE_MjaI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of the CTAG-recognizing restriction-modification systems MthZI and MthFI from Methanobacterium thermoformicicum and characterization of the plasmid-encoded mthZIM gene."
    Noelling J., de Vos W.M.
    Nucleic Acids Res. 20:5047-5052(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 3720 / Z-245.
  2. "Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum."
    Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.
    Nucleic Acids Res. 20:6501-6507(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 3720 / Z-245.

Entry informationi

Entry nameiT2MZ_METTF
AccessioniPrimary (citable) accession number: P29566
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 3, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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