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P29566

- T2MZ_METTF

UniProt

P29566 - T2MZ_METTF

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Protein

Probable type-2 restriction enzyme MthZI

Gene

mthZIR

Organism
Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CTAG and cleaves after C-1.1 Publication

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.1 Publication

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi2204. MthZI.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable type-2 restriction enzyme MthZI (EC:3.1.21.4)
Short name:
R.MthZI
Alternative name(s):
Endonuclease MthZI
Type II restriction enzyme MthZI
Gene namesi
Name:mthZIR
Encoded oniPlasmid pFZ10 Publication
OrganismiMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Taxonomic identifieri145262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Probable type-2 restriction enzyme MthZIPRO_0000077340Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019068. Restrct_endonuc_II_MjaI.
[Graphical view]
PfamiPF09568. RE_MjaI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29566-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIDNFKEIRL DFKDELKKIT GKEVEFPKYT TQIINLANQN AQGTRPRVVG
60 70 80 90 100
QMSDLIHECP DKSYEGWKKW YLEHYSDRIE KATKKISKMI ENMKAAMELI
110 120 130 140 150
DEEMIRKWVE DLVITKTAEG LIIQEIILKT IAEEAGLEWR LATSKEESKN
160 170 180 190 200
IDGFIGSTPV SIKPMSYESM RPTVREEIDI QTIFYKKPKN SRYLYIYHNL

NI
Length:202
Mass (Da):23,742
Last modified:April 1, 1993 - v1
Checksum:i1AC0274DEC882F49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67212 Genomic DNA. Translation: CAA47652.1.
X68367 Genomic DNA. Translation: CAA48446.1.
PIRiS26074.
RefSeqiNP_039774.1. NC_001337.1.
WP_010889861.1. NC_001337.1.

Genome annotation databases

GeneIDi3355220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67212 Genomic DNA. Translation: CAA47652.1 .
X68367 Genomic DNA. Translation: CAA48446.1 .
PIRi S26074.
RefSeqi NP_039774.1. NC_001337.1.
WP_010889861.1. NC_001337.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 2204. MthZI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3355220.

Family and domain databases

InterProi IPR019068. Restrct_endonuc_II_MjaI.
[Graphical view ]
Pfami PF09568. RE_MjaI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of the CTAG-recognizing restriction-modification systems MthZI and MthFI from Methanobacterium thermoformicicum and characterization of the plasmid-encoded mthZIM gene."
    Noelling J., de Vos W.M.
    Nucleic Acids Res. 20:5047-5052(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY.
    Strain: DSM 3720 / Z-245.
  2. "Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum."
    Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.
    Nucleic Acids Res. 20:6501-6507(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 3720 / Z-245.

Entry informationi

Entry nameiT2MZ_METTF
AccessioniPrimary (citable) accession number: P29566
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 1, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3