Reviewed, UniProtKB/Swiss-Prot P29566 (T2MZ_METTF)

Names and origin

Protein names

Recommended name:
    Probable type-2 restriction enzyme MthZI
      Short name=R.MthZI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme MthZI
    Endonuclease MthZI

Gene names

Name:

mthZIR

Encoded on

Plasmid pFZ1

Organism

Methanobacterium thermoformicicum

Taxonomic identifier

145262 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTAG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Probable type-2 restriction enzyme MthZI		PRO_0000077340

Sequences

	Sequence		Length	Mass (Da)
	P29566-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 1AC0274DEC882F49	FASTA	202	23,742
	10 20 30 40 50 60 MIDNFKEIRL DFKDELKKIT GKEVEFPKYT TQIINLANQN AQGTRPRVVG QMSDLIHECP 70 80 90 100 110 120 DKSYEGWKKW YLEHYSDRIE KATKKISKMI ENMKAAMELI DEEMIRKWVE DLVITKTAEG 130 140 150 160 170 180 LIIQEIILKT IAEEAGLEWR LATSKEESKN IDGFIGSTPV SIKPMSYESM RPTVREEIDI 190 200 QTIFYKKPKN SRYLYIYHNL NI

References

[1]	"Identification of the CTAG-recognizing restriction-modification systems MthZI and MthFI from Methanobacterium thermoformicicum and characterization of the plasmid-encoded mthZIM gene." Noelling J., de Vos W.M. Nucleic Acids Res. 20:5047-5052(1992) [PubMed: 1408820] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3720 / Z-245.
[2]	"Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum." Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M. Nucleic Acids Res. 20:6501-6507(1992) [PubMed: 1336177] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3720 / Z-245.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X67212 Genomic DNA. Translation: CAA47652.1. X68367 Genomic DNA. Translation: CAA48446.1.
PIR	S26074.
RefSeq	NP_039774.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	2204. MthZI.
Genome annotation databases
GeneID	3355220.
Enzyme and pathway databases
BRENDA	3.1.21.4. 258835.
Family and domain databases
InterPro	IPR019068. Restrict_endonuc_II_MjaI. [Graphical view]
Pfam	PF09568. RE_MjaI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2MZ_METTF

Accession

Primary (citable) accession number: P29566

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)