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Protein

Probable type-2 restriction enzyme MthZI

Gene

mthZIR

Organism
Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CTAG and cleaves after C-1.1 Publication

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system

Protein family/group databases

REBASEi2204. MthZI.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable type-2 restriction enzyme MthZI (EC:3.1.21.4)
Short name:
R.MthZI
Alternative name(s):
Endonuclease MthZI
Type II restriction enzyme MthZI
Gene namesi
Name:mthZIR
Encoded oniPlasmid pFZ10 Publication
OrganismiMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Taxonomic identifieri145262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773401 – 202Probable type-2 restriction enzyme MthZIAdd BLAST202

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiView protein in InterPro
IPR019068. Restrct_endonuc_II_MjaI.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF09568. RE_MjaI. 1 hit.

Sequencei

Sequence statusi: Complete.

P29566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDNFKEIRL DFKDELKKIT GKEVEFPKYT TQIINLANQN AQGTRPRVVG
60 70 80 90 100
QMSDLIHECP DKSYEGWKKW YLEHYSDRIE KATKKISKMI ENMKAAMELI
110 120 130 140 150
DEEMIRKWVE DLVITKTAEG LIIQEIILKT IAEEAGLEWR LATSKEESKN
160 170 180 190 200
IDGFIGSTPV SIKPMSYESM RPTVREEIDI QTIFYKKPKN SRYLYIYHNL

NI
Length:202
Mass (Da):23,742
Last modified:April 1, 1993 - v1
Checksum:i1AC0274DEC882F49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67212 Genomic DNA. Translation: CAA47652.1.
X68367 Genomic DNA. Translation: CAA48446.1.
PIRiS26074.
RefSeqiNP_039774.1. NC_001337.1.
WP_010889861.1. NC_001337.1.

Genome annotation databases

GeneIDi3355220.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiT2MZ_METTF
AccessioniPrimary (citable) accession number: P29566
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 7, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries