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P29566 (T2MZ_METTF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable type-2 restriction enzyme MthZI

Short name=R.MthZI
EC=3.1.21.4
Alternative name(s):
Endonuclease MthZI
Type II restriction enzyme MthZI
Gene names
Name:mthZIR
Encoded onPlasmid pFZ1
OrganismMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Taxonomic identifier145262 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTAG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 202202Probable type-2 restriction enzyme MthZI
PRO_0000077340

Sequences

Sequence LengthMass (Da)Tools
P29566 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 1AC0274DEC882F49

FASTA20223,742
        10         20         30         40         50         60 
MIDNFKEIRL DFKDELKKIT GKEVEFPKYT TQIINLANQN AQGTRPRVVG QMSDLIHECP 

        70         80         90        100        110        120 
DKSYEGWKKW YLEHYSDRIE KATKKISKMI ENMKAAMELI DEEMIRKWVE DLVITKTAEG 

       130        140        150        160        170        180 
LIIQEIILKT IAEEAGLEWR LATSKEESKN IDGFIGSTPV SIKPMSYESM RPTVREEIDI 

       190        200 
QTIFYKKPKN SRYLYIYHNL NI 

« Hide

References

[1]"Identification of the CTAG-recognizing restriction-modification systems MthZI and MthFI from Methanobacterium thermoformicicum and characterization of the plasmid-encoded mthZIM gene."
Noelling J., de Vos W.M.
Nucleic Acids Res. 20:5047-5052(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 3720 / Z-245.
[2]"Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum."
Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.
Nucleic Acids Res. 20:6501-6507(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 3720 / Z-245.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67212 Genomic DNA. Translation: CAA47652.1.
X68367 Genomic DNA. Translation: CAA48446.1.
PIRS26074.
RefSeqNP_039774.1. NC_001337.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2204. MthZI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3355220.

Family and domain databases

InterProIPR019068. Restrct_endonuc_II_MjaI.
[Graphical view]
PfamPF09568. RE_MjaI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2MZ_METTF
AccessionPrimary (citable) accession number: P29566
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries