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P29565 (T2MT_METTF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme MthTI

Short name=R.MthTI
EC=3.1.21.4
Alternative name(s):
Endonuclease MthTI
Type II restriction enzyme MthTI
Gene names
Name:mthTIR
Encoded onPlasmid pFV1
OrganismMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Taxonomic identifier145262 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGCC.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 281281Type-2 restriction enzyme MthTI
PRO_0000077339

Sequences

Sequence LengthMass (Da)Tools
P29565 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 083200CE4A7CD49A

FASTA28132,102
        10         20         30         40         50         60 
MSNLLQAIMN IKSIQERNLG SYKGVQDSKN RMNQMGVTLE VFLKDAFCNT FDIENKDLVY 

        70         80         90        100        110        120 
SEYFSYLGNQ NNPPDMILKG GDAVEVKKIT GIKTSIQLNS SYPKSKLFVS DSRITEACKN 

       130        140        150        160        170        180 
CEDWEVKDII YAIGTIPNRV LKLMFFVYGD CYAASPSIYQ RIVEEVKGGL HSTGLEFSET 

       190        200        210        220        230        240 
NELGRINRVD PLGITDLRVR GMWIIKHPIK VFKNIIPPES IKNNNFNLIA LMKAEKYKQF 

       250        260        270        280 
PKKDRKRIEA EDNIEVTDVK IKDPDNPAKL LDSVLVRYDE I 

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References

[1]"Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae."
Noelling J., de Vos W.M.
J. Bacteriol. 174:5719-5726(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 3848 / THF.
[2]"Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum."
Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.
Nucleic Acids Res. 20:6501-6507(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 3848 / THF.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M97222 Genomic DNA. Translation: AAA73369.1.
X68366 Genomic DNA. Translation: CAA48435.1.
PIRA42941.
RefSeqNP_039764.1. NC_001336.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1284. MthTI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2193585.

Family and domain databases

InterProIPR019046. Restrct_endonuc_II_NgoPII.
[Graphical view]
PfamPF09521. RE_NgoPII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2MT_METTF
AccessionPrimary (citable) accession number: P29565
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries