Reviewed, UniProtKB/Swiss-Prot P29565 (T2MT_METTF)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme MthTI
      Short name=R.MthTI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme MthTI
    Endonuclease MthTI

Gene names

Name:

mthTIR

Encoded on

Plasmid pFV1

Organism

Methanobacterium thermoformicicum

Taxonomic identifier

145262 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGCC.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Plasmid
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Type-2 restriction enzyme MthTI		PRO_0000077339

Sequences

	Sequence		Length	Mass (Da)
	P29565-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 083200CE4A7CD49A	FASTA	281	32,102
	10 20 30 40 50 60 MSNLLQAIMN IKSIQERNLG SYKGVQDSKN RMNQMGVTLE VFLKDAFCNT FDIENKDLVY 70 80 90 100 110 120 SEYFSYLGNQ NNPPDMILKG GDAVEVKKIT GIKTSIQLNS SYPKSKLFVS DSRITEACKN 130 140 150 160 170 180 CEDWEVKDII YAIGTIPNRV LKLMFFVYGD CYAASPSIYQ RIVEEVKGGL HSTGLEFSET 190 200 210 220 230 240 NELGRINRVD PLGITDLRVR GMWIIKHPIK VFKNIIPPES IKNNNFNLIA LMKAEKYKQF 250 260 270 280 PKKDRKRIEA EDNIEVTDVK IKDPDNPAKL LDSVLVRYDE I

References

[1]	"Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae." Noelling J., de Vos W.M. J. Bacteriol. 174:5719-5726(1992) [PubMed: 1512204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3848 / THF.
[2]	"Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum." Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M. Nucleic Acids Res. 20:6501-6507(1992) [PubMed: 1336177] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3848 / THF.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M97222 Genomic DNA. Translation: AAA73369.1. X68366 Genomic DNA. Translation: CAA48435.1.
PIR	A42941.
RefSeq	NP_039764.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1284. MthTI.
Genome annotation databases
GeneID	2193585.
Enzyme and pathway databases
BRENDA	3.1.21.4. 258835.
Family and domain databases
InterPro	IPR019046. Restrict_endonuc_II_NgoPII. [Graphical view]
Pfam	PF09521. RE_NgoPII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2MT_METTF

Accession

Primary (citable) accession number: P29565

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)