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P29565

- T2MT_METTF

UniProt

P29565 - T2MT_METTF

Protein

Type-2 restriction enzyme MthTI

Gene

mthTIR

Organism
Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GGCC.1 Publication

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.1 Publication

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1284. MthTI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme MthTI (EC:3.1.21.4)
    Short name:
    R.MthTI
    Alternative name(s):
    Endonuclease MthTI
    Type II restriction enzyme MthTI
    Gene namesi
    Name:mthTIR
    Encoded oniPlasmid pFV10 Publication
    OrganismiMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
    Taxonomic identifieri145262 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281Type-2 restriction enzyme MthTIPRO_0000077339Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019046. Restrct_endonuc_II_NgoPII.
    [Graphical view]
    PfamiPF09521. RE_NgoPII. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29565-1 [UniParc]FASTAAdd to Basket

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    MSNLLQAIMN IKSIQERNLG SYKGVQDSKN RMNQMGVTLE VFLKDAFCNT    50
    FDIENKDLVY SEYFSYLGNQ NNPPDMILKG GDAVEVKKIT GIKTSIQLNS 100
    SYPKSKLFVS DSRITEACKN CEDWEVKDII YAIGTIPNRV LKLMFFVYGD 150
    CYAASPSIYQ RIVEEVKGGL HSTGLEFSET NELGRINRVD PLGITDLRVR 200
    GMWIIKHPIK VFKNIIPPES IKNNNFNLIA LMKAEKYKQF PKKDRKRIEA 250
    EDNIEVTDVK IKDPDNPAKL LDSVLVRYDE I 281
    Length:281
    Mass (Da):32,102
    Last modified:April 1, 1993 - v1
    Checksum:i083200CE4A7CD49A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97222 Genomic DNA. Translation: AAA73369.1.
    X68366 Genomic DNA. Translation: CAA48435.1.
    PIRiA42941.
    RefSeqiNP_039764.1. NC_001336.1.
    WP_010889850.1. NC_001336.1.

    Genome annotation databases

    GeneIDi2193585.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97222 Genomic DNA. Translation: AAA73369.1 .
    X68366 Genomic DNA. Translation: CAA48435.1 .
    PIRi A42941.
    RefSeqi NP_039764.1. NC_001336.1.
    WP_010889850.1. NC_001336.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1284. MthTI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2193585.

    Family and domain databases

    InterProi IPR019046. Restrct_endonuc_II_NgoPII.
    [Graphical view ]
    Pfami PF09521. RE_NgoPII. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae."
      Noelling J., de Vos W.M.
      J. Bacteriol. 174:5719-5726(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY.
      Strain: DSM 3848 / THF.
    2. "Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum."
      Noelling J., van Eeden F.J.M., Eggen R.I.L., de Vos W.M.
      Nucleic Acids Res. 20:6501-6507(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 3848 / THF.

    Entry informationi

    Entry nameiT2MT_METTF
    AccessioniPrimary (citable) accession number: P29565
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3