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Protein

Type-2 restriction enzyme MthTI

Gene

mthTIR

Organism
Methanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GGCC.1 Publication

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system

Protein family/group databases

REBASEi1284. MthTI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MthTI (EC:3.1.21.4)
Short name:
R.MthTI
Alternative name(s):
Endonuclease MthTI
Type II restriction enzyme MthTI
Gene namesi
Name:mthTIR
Encoded oniPlasmid pFV10 Publication
OrganismiMethanothermobacter thermautotrophicus (Methanobacterium thermoformicicum)
Taxonomic identifieri145262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773391 – 281Type-2 restriction enzyme MthTIAdd BLAST281

Structurei

3D structure databases

SMRiP29565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

InterProiView protein in InterPro
IPR019046. Restrct_endonuc_II_NgoPII.
PfamiView protein in Pfam
PF09521. RE_NgoPII. 1 hit.

Sequencei

Sequence statusi: Complete.

P29565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLLQAIMN IKSIQERNLG SYKGVQDSKN RMNQMGVTLE VFLKDAFCNT
60 70 80 90 100
FDIENKDLVY SEYFSYLGNQ NNPPDMILKG GDAVEVKKIT GIKTSIQLNS
110 120 130 140 150
SYPKSKLFVS DSRITEACKN CEDWEVKDII YAIGTIPNRV LKLMFFVYGD
160 170 180 190 200
CYAASPSIYQ RIVEEVKGGL HSTGLEFSET NELGRINRVD PLGITDLRVR
210 220 230 240 250
GMWIIKHPIK VFKNIIPPES IKNNNFNLIA LMKAEKYKQF PKKDRKRIEA
260 270 280
EDNIEVTDVK IKDPDNPAKL LDSVLVRYDE I
Length:281
Mass (Da):32,102
Last modified:April 1, 1993 - v1
Checksum:i083200CE4A7CD49A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97222 Genomic DNA. Translation: AAA73369.1.
X68366 Genomic DNA. Translation: CAA48435.1.
PIRiA42941.
RefSeqiNP_039764.1. NC_001336.1.
WP_010889850.1. NC_001336.1.

Genome annotation databases

GeneIDi2193585.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiT2MT_METTF
AccessioniPrimary (citable) accession number: P29565
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: March 15, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries