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P29564

- SUMT_METIV

UniProt

P29564 - SUMT_METIV

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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

cobA

Organism
Methanobacterium ivanovii
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei115 – 1151S-adenosyl-L-methionineBy similarity
Binding sitei166 – 1661S-adenosyl-L-methionine; via amide nitrogenBy similarity

GO - Molecular functioni

  1. precorrin-2 dehydrogenase activity Source: InterPro
  2. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
  2. porphyrin-containing compound biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12172.
UniPathwayiUPA00148; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:cobA
Synonyms:corA
OrganismiMethanobacterium ivanovii
Taxonomic identifieri2163 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 231230Uroporphyrinogen-III C-methyltransferasePRO_0000150370Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP29564.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni85 – 873S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29564-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVVYLVGAGP GDPELITLKA VNVLKKADVV LYDKPANEEI LKYAEGAKLI
60 70 80 90 100
YVGKQAGHHY KSQNEINTLL VEEAKENDLV VRLKGGDPFV FGRGGEEILA
110 120 130 140 150
LVEEGIDFEL VPGVTSAIGV PTTIGLPVTH RGVATSFTVV TGHEDPTKCK
160 170 180 190 200
KQVGWDFKAD TIVILMGIGN LAENTAEIMK HKDPETPVCV IENGTMEGQR
210 220 230
IITGTLENIA GKDIKPPALV VLEMLSMFLK K
Length:231
Mass (Da):24,916
Last modified:January 23, 2007 - v2
Checksum:i6EB100DAA96EB37A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62874 Genomic DNA. Translation: AAA72997.1.
PIRiA42471.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62874 Genomic DNA. Translation: AAA72997.1 .
PIRi A42471.

3D structure databases

ProteinModelPortali P29564.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
BioCyci MetaCyc:MONOMER-12172.

Family and domain databases

Gene3Di 3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF00590. TP_methylase. 1 hit.
[Graphical view ]
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification, characterization, and molecular cloning of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Methanobacterium ivanovii."
    Blanche F., Robin C., Couder M., Faucher D., Cauchois L., Cameron B., Crouzet J.
    J. Bacteriol. 173:4637-4645(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31 AND 201-212.
    Strain: DSM 2611.

Entry informationi

Entry nameiSUMT_METIV
AccessioniPrimary (citable) accession number: P29564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3