P29558 (RBMS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding motif, single-stranded-interacting protein 1

Alternative name(s):
Single-stranded DNA-binding protein MSSP-1
Suppressor of CDC2 with RNA-binding motif 2

Gene names

Name:	RBMS1
Synonyms:	C2orf12, MSSP, MSSP1, SCR2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Single-stranded DNA binding protein that interacts with the region upstream of the MYC gene. Binds specifically to the DNA sequence motif 5'-[AT]CT[AT][AT]T-3'. Probably has a role in DNA replication.
Subcellular location	Nucleus.
Tissue specificity	Highest amounts are found in placenta, lung and heart.
Developmental stage	Expressed at highest levels during the G1 to S transition of the cell cycle.
Sequence similarities	Contains 2 RRM (RNA recognition motif) domains.
Sequence caution	The sequence AAX93095.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA45923.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAA45923.1 differs from that shown. Reason: Frameshift at positions 319 and 360. The sequence CAA54628.1 differs from that shown. Reason: Erroneous translation. Wrong coding sequence.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	DNA-binding RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Non-traceable author statement Ref.2. Source: UniProtKB RNA processing Traceable author statement. Source: ProtInc
Cellular component	nucleus Non-traceable author statement Ref.2. Source: UniProtKB
Molecular function	RNA binding Non-traceable author statement Ref.1. Source: UniProtKB double-stranded DNA binding Non-traceable author statement Ref.2. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: UniProtKB single-stranded DNA binding Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P29558-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P29558-2)

The sequence of this isoform differs from the canonical sequence as follows:
     250-252: Missing.

Isoform 4 (identifier: P29558-4)

Also known as: MSSP-2;

The sequence of this isoform differs from the canonical sequence as follows:
     26-38: QSLVPAHPMAPPS → FGRHSGIPSEKEE
     39-406: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 406

406

RNA-binding motif, single-stranded-interacting protein 1

PRO_0000081798

Regions

Domain

62 – 135

74

RRM 1

Domain

141 – 226

86

RRM 2

Amino acid modifications

Modified residue

112

1

Phosphoserine Ref.6 Ref.7 Ref.8

Modified residue

208

1

Phosphothreonine Ref.6

Natural variations

Alternative sequence

26 – 38

13

QSLVP…MAPPS → FGRHSGIPSEKEE in isoform 4.

VSP_037790

Alternative sequence

39 – 406

368

Missing in isoform 4.

VSP_037791

Alternative sequence

250 – 252

3

Missing in isoform 2.

VSP_011930

Experimental info

Sequence conflict

132

1

M → K in CAA45923. Ref.5

Sequence conflict

315 – 316

2

QH → HD in CAA54628. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

406

Helix Strand Turn

Details...

Beta strand

142 – 147

6

Helix

154 – 159

6

Turn

160 – 164

5

Beta strand

167 – 174

8

Beta strand

176 – 178

3

Beta strand

180 – 189

10

Helix

191 – 201

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 23, 2004. Version 3. Checksum: 97EAAD2B0A5E01C6	FASTA	406	44,505
	10 20 30 40 50 60 MGKVWKQQMY PQYATYYYPQ YLQAKQSLVP AHPMAPPSPS TTSSNNNSSS SSNSGWDQLS 70 80 90 100 110 120 KTNLYIRGLP PHTTDQDLVK LCQPYGKIVS TKAILDKTTN KCKGYGFVDF DSPAAAQKAV 130 140 150 160 170 180 SALKASGVQA QMAKQQEQDP TNLYISNLPL SMDEQELENM LKPFGQVIST RILRDSSGTS 190 200 210 220 230 240 RGVGFARMES TEKCEAVIGH FNGKFIKTPP GVSAPTEPLL CKFADGGQKK RQNPNKYIPN 250 260 270 280 290 300 GRPWHREGEV RLAGMTLTYD PTTAAIQNGF YPSPYSIATN RMITQTSITP YIASPVSAYQ 310 320 330 340 350 360 VQSPSWMQPQ PYILQHPGAV LTPSMEHTMS LQPASMISPL AQQMSHLSLG STGTYMPATS 370 380 390 400 AMQGAYLPQY AHMQTTAVPV EEASGQQQVA VETSNDHSPY TFQPNK
	Isoform 2 [UniParc]. Checksum: 614911BB16E670B1	FASTA	403	44,137
	10 20 30 40 50 60 MGKVWKQQMY PQYATYYYPQ YLQAKQSLVP AHPMAPPSPS TTSSNNNSSS SSNSGWDQLS 70 80 90 100 110 120 KTNLYIRGLP PHTTDQDLVK LCQPYGKIVS TKAILDKTTN KCKGYGFVDF DSPAAAQKAV 130 140 150 160 170 180 SALKASGVQA QMAKQQEQDP TNLYISNLPL SMDEQELENM LKPFGQVIST RILRDSSGTS 190 200 210 220 230 240 RGVGFARMES TEKCEAVIGH FNGKFIKTPP GVSAPTEPLL CKFADGGQKK RQNPNKYIPN 250 260 270 280 290 300 GRPWHREGEA GMTLTYDPTT AAIQNGFYPS PYSIATNRMI TQTSITPYIA SPVSAYQVQS 310 320 330 340 350 360 PSWMQPQPYI LQHPGAVLTP SMEHTMSLQP ASMISPLAQQ MSHLSLGSTG TYMPATSAMQ 370 380 390 400 GAYLPQYAHM QTTAVPVEEA SGQQQVAVET SNDHSPYTFQ PNK
	Isoform 4 (MSSP-2) [UniParc]. Checksum: 6D0758AEEAA3012B	FASTA	38	4,632
	10 20 30 MGKVWKQQMY PQYATYYYPQ YLQAKFGRHS GIPSEKEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SCR: novel human suppresors of cdc2/cdc13 mutants of Schizosaccharomyces pombe harbour motifs for RNA binding proteins." Kanaoka Y., Nojima H. Nucleic Acids Res. 22:2687-2693(1994) [PubMed: 8041632] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Molecular cloning of MSSP-2, a c-myc gene single-strand binding protein: characterization of binding specificity and DNA replication activity." Takai T., Nishita Y., Iguchi-Ariga S.M.M., Ariga H. Nucleic Acids Res. 22:5576-5581(1994) [PubMed: 7838710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Muscle.
[5]	"Identification and cDNA cloning of single-stranded DNA binding proteins that interact with the region upstream of the human c-myc gene." Negishi Y., Nishita Y., Saegusa Y., Kakizaki I., Galli I., Kihara F., Tamai K., Miyajima N., Iguchi-Ariga S.M.M., Ariga H. Oncogene 9:1133-1143(1994) [PubMed: 8134115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-406 (ISOFORM 1).
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112 AND THR-208, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"Solution structure of RRM domain in RNA binding motif, single-stranded-interacting protein 1." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 121-223.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D28482 mRNA. Translation: BAA05841.1.
X77494 mRNA. Translation: CAA54628.1. Sequence problems.
AC092153 Genomic DNA. Translation: AAX93095.1. Sequence problems.
AC104598 Genomic DNA. Translation: AAX93183.1.
AC131754 Genomic DNA. Translation: AAY15005.1.
BC012992 mRNA. Translation: AAH12992.1.
BC012993 mRNA. Translation: AAH12993.1.
BC018951 mRNA. Translation: AAH18951.1.
X64652 mRNA. Translation: CAA45923.1. Sequence problems.

IPI

IPI00033611.
IPI00929296.
IPI00941661.

PIR

I38042.
S19873.
S47659.
S53612.

RefSeq

NP_002888.1. NM_002897.4.
NP_058520.1. NM_016836.3.

UniGene

Hs.470412.
Hs.708357.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X5O	NMR	-	A	123-223	[»]

ProteinModelPortal

P29558.

SMR

P29558. Positions 60-228.

ModBase

Search...

Protein-protein interaction databases

STRING

P29558.

PTM databases

PhosphoSite

P29558.

Polymorphism databases

DMDM

55977783.

Proteomic databases

PRIDE

P29558.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000348849; ENSP00000294904; ENSG00000153250.

GeneID

5937.

KEGG

hsa:5937.

UCSC

uc002ubk.1. human.
uc002ubo.1. human.

Organism-specific databases

CTD

5937.

GeneCards

GC02M161128.

H-InvDB

HIX0002538.

HGNC

HGNC:9907. RBMS1.

MIM

602310. gene.

neXtProt

NX_P29558.

PharmGKB

PA34273.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG16892.

HOVERGEN

HBG053108.

InParanoid

P29558.

OMA

WHREGEA.

OrthoDB

EOG469QV2.

PhylomeDB

P29558.

Gene expression databases

ArrayExpress

P29558.

Bgee

P29558.

CleanEx

HS_RBMS1.

Genevestigator

P29558.

GermOnline

ENSG00000153250. Homo sapiens.

Family and domain databases

InterPro

IPR002343. Hud_Sxl_RNA.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

Gene3D

G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.

Pfam

PF00076. RRM_1. 2 hits.
[Graphical view]

PRINTS

PR00961. HUDSXLRNA.

SMART

SM00360. RRM. 2 hits.
[Graphical view]

PROSITE

PS50102. RRM. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

23144.

SOURCE

Search...

Entry information

Entry name

RBMS1_HUMAN

Accession

Primary (citable) accession number: P29558
Secondary accession number(s): Q14869 , Q15433, Q53P46, Q53QX8, Q53RG6, Q8WV20

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.