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Protein

RNA-binding motif, single-stranded-interacting protein 1

Gene

RBMS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded DNA binding protein that interacts with the region upstream of the MYC gene. Binds specifically to the DNA sequence motif 5'-[AT]CT[AT][AT]T-3'. Probably has a role in DNA replication.

GO - Molecular functioni

  • double-stranded DNA binding Source: UniProtKB
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • single-stranded DNA binding Source: UniProtKB

GO - Biological processi

  • DNA replication Source: UniProtKB
  • RNA processing Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000153250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding motif, single-stranded-interacting protein 1
Alternative name(s):
Single-stranded DNA-binding protein MSSP-1
Suppressor of CDC2 with RNA-binding motif 2
Gene namesi
Name:RBMS1
Synonyms:C2orf12, MSSP, MSSP1, SCR2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:9907. RBMS1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5937.
OpenTargetsiENSG00000153250.
PharmGKBiPA34273.

Polymorphism and mutation databases

BioMutaiRBMS1.
DMDMi55977783.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817981 – 406RNA-binding motif, single-stranded-interacting protein 1Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei208PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP29558.
MaxQBiP29558.
PaxDbiP29558.
PeptideAtlasiP29558.
PRIDEiP29558.

PTM databases

iPTMnetiP29558.
PhosphoSitePlusiP29558.

Expressioni

Tissue specificityi

Highest amounts are found in placenta, lung and heart.

Developmental stagei

Expressed at highest levels during the G1 to S transition of the cell cycle.

Gene expression databases

BgeeiENSG00000153250.
CleanExiHS_RBMS1.
ExpressionAtlasiP29558. baseline and differential.
GenevisibleiP29558. HS.

Organism-specific databases

HPAiHPA047943.
HPA061791.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TFCP2Q128003EBI-5462600,EBI-717422

Protein-protein interaction databases

BioGridi111872. 39 interactors.
IntActiP29558. 5 interactors.
MINTiMINT-3046865.
STRINGi9606.ENSP00000294904.

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi142 – 147Combined sources6
Helixi154 – 159Combined sources6
Turni160 – 164Combined sources5
Beta strandi167 – 174Combined sources8
Beta strandi176 – 178Combined sources3
Beta strandi180 – 189Combined sources10
Helixi191 – 201Combined sources11
Beta strandi219 – 223Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X5ONMR-A123-223[»]
ProteinModelPortaliP29558.
SMRiP29558.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29558.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 135RRM 1PROSITE-ProRule annotationAdd BLAST74
Domaini141 – 226RRM 2PROSITE-ProRule annotationAdd BLAST86

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4733. Eukaryota.
ENOG410XRB5. LUCA.
GeneTreeiENSGT00760000118913.
HOVERGENiHBG053108.
InParanoidiP29558.
OMAiKVQSPSW.
OrthoDBiEOG091G0FXS.
PhylomeDBiP29558.
TreeFamiTF314644.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR002343. Hud_Sxl_RNA.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
PRINTSiPR00961. HUDSXLRNA.
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P29558-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKVWKQQMY PQYATYYYPQ YLQAKQSLVP AHPMAPPSPS TTSSNNNSSS
60 70 80 90 100
SSNSGWDQLS KTNLYIRGLP PHTTDQDLVK LCQPYGKIVS TKAILDKTTN
110 120 130 140 150
KCKGYGFVDF DSPAAAQKAV SALKASGVQA QMAKQQEQDP TNLYISNLPL
160 170 180 190 200
SMDEQELENM LKPFGQVIST RILRDSSGTS RGVGFARMES TEKCEAVIGH
210 220 230 240 250
FNGKFIKTPP GVSAPTEPLL CKFADGGQKK RQNPNKYIPN GRPWHREGEV
260 270 280 290 300
RLAGMTLTYD PTTAAIQNGF YPSPYSIATN RMITQTSITP YIASPVSAYQ
310 320 330 340 350
VQSPSWMQPQ PYILQHPGAV LTPSMEHTMS LQPASMISPL AQQMSHLSLG
360 370 380 390 400
STGTYMPATS AMQGAYLPQY AHMQTTAVPV EEASGQQQVA VETSNDHSPY

TFQPNK
Length:406
Mass (Da):44,505
Last modified:November 23, 2004 - v3
Checksum:i97EAAD2B0A5E01C6
GO
Isoform 2 (identifier: P29558-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     250-252: Missing.

Show »
Length:403
Mass (Da):44,137
Checksum:i614911BB16E670B1
GO
Isoform 4 (identifier: P29558-4) [UniParc]FASTAAdd to basket
Also known as: MSSP-2

The sequence of this isoform differs from the canonical sequence as follows:
     26-38: QSLVPAHPMAPPS → FGRHSGIPSEKEE
     39-406: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:38
Mass (Da):4,632
Checksum:i6D0758AEEAA3012B
GO

Sequence cautioni

The sequence AAX93095 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA45923 differs from that shown. Reason: Frameshift at positions 319 and 360.Curated
The sequence CAA45923 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA54628 differs from that shown. Reason: Erroneous translation. Wrong coding sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132M → K in CAA45923 (PubMed:8134115).Curated1
Sequence conflicti315 – 316QH → HD in CAA54628 (PubMed:7838710).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03779026 – 38QSLVP…MAPPS → FGRHSGIPSEKEE in isoform 4. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_03779139 – 406Missing in isoform 4. 1 PublicationAdd BLAST368
Alternative sequenceiVSP_011930250 – 252Missing in isoform 2. 2 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28482 mRNA. Translation: BAA05841.1.
X77494 mRNA. Translation: CAA54628.1. Sequence problems.
AC092153 Genomic DNA. Translation: AAX93095.1. Sequence problems.
AC104598 Genomic DNA. Translation: AAX93183.1.
AC131754 Genomic DNA. Translation: AAY15005.1.
BC012992 mRNA. Translation: AAH12992.1.
BC012993 mRNA. Translation: AAH12993.1.
BC018951 mRNA. Translation: AAH18951.1.
X64652 mRNA. Translation: CAA45923.1. Sequence problems.
CCDSiCCDS2213.1. [P29558-1]
PIRiI38042.
S19873.
S47659.
S53612.
RefSeqiNP_002888.1. NM_002897.4. [P29558-2]
NP_058520.1. NM_016836.3. [P29558-1]
UniGeneiHs.470412.
Hs.708357.

Genome annotation databases

EnsembliENST00000348849; ENSP00000294904; ENSG00000153250. [P29558-1]
GeneIDi5937.
KEGGihsa:5937.
UCSCiuc002ubo.4. human. [P29558-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28482 mRNA. Translation: BAA05841.1.
X77494 mRNA. Translation: CAA54628.1. Sequence problems.
AC092153 Genomic DNA. Translation: AAX93095.1. Sequence problems.
AC104598 Genomic DNA. Translation: AAX93183.1.
AC131754 Genomic DNA. Translation: AAY15005.1.
BC012992 mRNA. Translation: AAH12992.1.
BC012993 mRNA. Translation: AAH12993.1.
BC018951 mRNA. Translation: AAH18951.1.
X64652 mRNA. Translation: CAA45923.1. Sequence problems.
CCDSiCCDS2213.1. [P29558-1]
PIRiI38042.
S19873.
S47659.
S53612.
RefSeqiNP_002888.1. NM_002897.4. [P29558-2]
NP_058520.1. NM_016836.3. [P29558-1]
UniGeneiHs.470412.
Hs.708357.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X5ONMR-A123-223[»]
ProteinModelPortaliP29558.
SMRiP29558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111872. 39 interactors.
IntActiP29558. 5 interactors.
MINTiMINT-3046865.
STRINGi9606.ENSP00000294904.

PTM databases

iPTMnetiP29558.
PhosphoSitePlusiP29558.

Polymorphism and mutation databases

BioMutaiRBMS1.
DMDMi55977783.

Proteomic databases

EPDiP29558.
MaxQBiP29558.
PaxDbiP29558.
PeptideAtlasiP29558.
PRIDEiP29558.

Protocols and materials databases

DNASUi5937.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000348849; ENSP00000294904; ENSG00000153250. [P29558-1]
GeneIDi5937.
KEGGihsa:5937.
UCSCiuc002ubo.4. human. [P29558-1]

Organism-specific databases

CTDi5937.
DisGeNETi5937.
GeneCardsiRBMS1.
HGNCiHGNC:9907. RBMS1.
HPAiHPA047943.
HPA061791.
MIMi602310. gene.
neXtProtiNX_P29558.
OpenTargetsiENSG00000153250.
PharmGKBiPA34273.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4733. Eukaryota.
ENOG410XRB5. LUCA.
GeneTreeiENSGT00760000118913.
HOVERGENiHBG053108.
InParanoidiP29558.
OMAiKVQSPSW.
OrthoDBiEOG091G0FXS.
PhylomeDBiP29558.
TreeFamiTF314644.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000153250-MONOMER.

Miscellaneous databases

ChiTaRSiRBMS1. human.
EvolutionaryTraceiP29558.
GeneWikiiRBMS1.
GenomeRNAii5937.
PROiP29558.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000153250.
CleanExiHS_RBMS1.
ExpressionAtlasiP29558. baseline and differential.
GenevisibleiP29558. HS.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR002343. Hud_Sxl_RNA.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
PRINTSiPR00961. HUDSXLRNA.
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBMS1_HUMAN
AccessioniPrimary (citable) accession number: P29558
Secondary accession number(s): Q14869
, Q15433, Q53P46, Q53QX8, Q53RG6, Q8WV20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 23, 2004
Last modified: November 2, 2016
This is version 159 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.