Reviewed, UniProtKB/Swiss-Prot P29555 (ABDA_DROME)

Names and origin

Protein names

Recommended name:
    Homeobox protein abdominal-A

Gene names

Name:	abd-A
ORF Names:	CG10325

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for segmental identity of the second through eighth abdominal segments. Once a pattern of abd-A expression is turned on in a given parasegment, it remains on the more posterior parasegment, so that the complex pattern of expression is built up in the successive parasegments. Appears to repress expression of Ubx whenever they appear in the same cell, but abd-A is repressed by Abd-B only in the eight and ninth abdominal segments. Ref.1
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the Antp homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from mutant phenotype. Source: FlyBase cardiac muscle tissue development Inferred from mutant phenotype. Source: FlyBase cardioblast differentiation Inferred from mutant phenotype. Source: FlyBase determination of genital disc primordium Inferred from mutant phenotype. Source: FlyBase dorsal vessel heart proper cell fate commitment Inferred from mutant phenotype. Source: FlyBase genital disc anterior/posterior pattern formation Inferred from expression pattern. Source: FlyBase genitalia development Inferred from mutant phenotype. Source: FlyBase gonadal mesoderm development Inferred from mutant phenotype. Source: FlyBase mesodermal cell fate specification Inferred from mutant phenotype. Source: FlyBase midgut development Traceable author statement. Source: FlyBase oenocyte development Inferred from mutant phenotype. Source: FlyBase peripheral nervous system development Traceable author statement. Source: FlyBase pole cell migration Traceable author statement. Source: FlyBase positive regulation of muscle organ development Inferred from mutant phenotype. Source: FlyBase regulation of transcription, DNA-dependent Ref.1 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Ref.1 Inferred from mutant phenotype. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Abd-A2 (identifier: P29555-1)

Also known as: ABD-AII; B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Abd-A1 (identifier: P29555-2)

Also known as: ABD-AI; A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-260: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Homeobox protein abdominal-A		PRO_0000200249
Regions
	DNA binding	398 – 457	60	Homeobox
	Motif	368 – 373	6	Antp-type hexapeptide
	Compositional bias	35 – 50	16	Poly-Ala
	Compositional bias	51 – 119	69	Ser-rich
	Compositional bias	136 – 139	4	Poly-Gln (OPA repeat)
	Compositional bias	144 – 147	4	Poly-Gln (OPA repeat)
	Compositional bias	160 – 165	6	Poly-Gln (OPA repeat)
	Compositional bias	172 – 177	6	Poly-Ala
	Compositional bias	240 – 250	11	Poly-Ala
	Compositional bias	425 – 428	4	Poly-Arg
	Compositional bias	491 – 518	28	Poly-Gln (OPA repeat)
Natural variations
	Alternative sequence	1 – 260	260	Missing in isoform Abd-A1.		VSP_002394

Sequences

	Sequence		Length	Mass (Da)
	Isoform Abd-A2 (ABD-AII) (B) [UniParc]. Last modified October 1, 1996. Version 2. Checksum: FF080CC2D71ECA82	FASTA	590	62,410
	10 20 30 40 50 60 MSKFVFDSML PKYPQFQPFI SSHHLTTTPP NSSSAAVAAA LAAAAASASA SVSASSSSNN 70 80 90 100 110 120 NSSNTIAGSN TSNTNNSSSS PSSSSNNNSN LNLSGGSLSP SHLSQHLGQS PHSPVSSSSP 130 140 150 160 170 180 FQQHHPQVQQ QHLNHQQQQH LHHQQQQHHH QYSSLSAALQ LQQQQHHISK LAAAAVASHG 190 200 210 220 230 240 HAHQQLLLTP PSAGNSQAGD SSCSPSPSAS GSSSLHRSLN DNSPGSASAS ASASAASSVA 250 260 270 280 290 300 AAAAAAAAAA SSSFAIPTSK MYPYVSNHPS SHGGLSGMAG FTGLEDKSCS RYTDTVMNSY 310 320 330 340 350 360 QSMSVPASAS AQFAQFYQHA TAAASAVSAA SAGAIGVDSL GNACTQPASG VMPGAGGAGG 370 380 390 400 410 420 AGIADLPRYP WMTLTDWMGS PFERVVCGDF NGPNGCPRRR GRQTYTRFQT LELEKEFHFN 430 440 450 460 470 480 HYLTRRRRIE IAHALCLTER QIKIWFQNRR MKLKKELRAV KEINEQARRD REEQEKMKAQ 490 500 510 520 530 540 ETMKSAQQNK QVQQQQQQQQ QQQQQQQQQH QQQQQQPQDH HSIIAHNPGH LHHSVVGQND 550 560 570 580 590 LKLGLGMGVG VGVGGIGPGI GGGLGGNLGM MSALDKSNHD LLKAVSKVNS
	Isoform Abd-A1 (ABD-AI) (A). Checksum: 30C665D1F817AA10	FASTA	330	36,180
	10 20 30 40 50 60 MYPYVSNHPS SHGGLSGMAG FTGLEDKSCS RYTDTVMNSY QSMSVPASAS AQFAQFYQHA 70 80 90 100 110 120 TAAASAVSAA SAGAIGVDSL GNACTQPASG VMPGAGGAGG AGIADLPRYP WMTLTDWMGS 130 140 150 160 170 180 PFERVVCGDF NGPNGCPRRR GRQTYTRFQT LELEKEFHFN HYLTRRRRIE IAHALCLTER 190 200 210 220 230 240 QIKIWFQNRR MKLKKELRAV KEINEQARRD REEQEKMKAQ ETMKSAQQNK QVQQQQQQQQ 250 260 270 280 290 300 QQQQQQQQQH QQQQQQPQDH HSIIAHNPGH LHHSVVGQND LKLGLGMGVG VGVGGIGPGI 310 320 330 GGGLGGNLGM MSALDKSNHD LLKAVSKVNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"abdA expression in Drosophila embryos." Karch F., Bender W., Weiffenbach B. Genes Dev. 4:1573-1587(1990) [PubMed: 1979297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ABD-A1), FUNCTION, SUBCELLULAR LOCATION.
[2]	"Complete sequence of the bithorax complex of Drosophila." Martin C.H., Mayeda C.A., Davis C.A., Ericsson C.L., Knafels J.D., Mathog D.R., Celniker S.E., Lewis E.B., Palazzolo M.J. Proc. Natl. Acad. Sci. U.S.A. 92:8398-8402(1995) [PubMed: 7667301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS ABD-A1 AND ABD-A2). Strain: Canton-S.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ABD-A1). Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54453 mRNA. Translation: CAA38321.1. U31961 Genomic DNA. Translation: AAA84405.1. U31961 Genomic DNA. Translation: AAA84406.1. AE014297 Genomic DNA. Translation: AAF55359.1. AE014297 Genomic DNA. Translation: AAF55360.2. BT016031 mRNA. Translation: AAV36916.1.
PIR	A35915.
RefSeq	NP_476693.1. NP_732176.1.
UniGene	Dm.3286
3D structure databases
SMR	P29555. Positions 398-456.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-17987N.
IntAct	P29555. 2 interactions.
STRING	P29555.
Genome annotation databases
Ensembl	FBtr0083387; FBpp0082828; FBgn0000014; Drosophila melanogaster. [Genome view] FBtr0083388; FBpp0082829; FBgn0000014; Drosophila melanogaster. [Genome view] FBtr0300485; FBpp0289712; FBgn0000014; Drosophila melanogaster. [Genome view]
GeneID	42037.
KEGG	dme:Dmel_CG10325.
NMPDR	fig|7227.3.peg.13206.
Organism-specific databases
CTD	42037.
FlyBase	FBgn0000014. abd-A.
Phylogenomic databases
InParanoid	P29555.
OMA	HHISKLA.
OrthoDB	EOG91C72V.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-011989-MON.
Gene expression databases
ArrayExpress	P29555.
GermOnline	CG10325. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR020479. Homeobox_region. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00032. ANTENNAPEDIA. False negative. PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	826865.

Entry information

Entry name

ABDA_DROME

Accession

Primary (citable) accession number: P29555
Secondary accession number(s): Q5U161, Q9VER1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	October 1, 1996
Last modified:	December 15, 2009
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project