Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Elongation factor 1-gamma 1

Gene

CAM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the eukaryotic elongation factor 1 complex (eEF1). Probably plays a role in anchoring the complex to other cellular components. May be involved in transcriptional regulation of MXR1.1 Publication

Pathwayi: polypeptide chain elongation

This protein is involved in the pathway polypeptide chain elongation, which is part of Protein biosynthesis.
View all proteins of this organism that are known to be involved in the pathway polypeptide chain elongation and in Protein biosynthesis.

GO - Molecular functioni

  • calcium ion binding Source: SGD
  • core promoter binding Source: SGD
  • guanyl-nucleotide exchange factor activity Source: SGD
  • phospholipid binding Source: SGD
  • transcription factor activity, sequence-specific DNA binding Source: SGD
  • translation elongation factor activity Source: GO_Central

GO - Biological processi

  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of translational termination Source: SGD
  • ribosome biogenesis Source: SGD
  • translational elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-33961-MONOMER.
ReactomeiR-SCE-156842. Eukaryotic Translation Elongation.
UniPathwayiUPA00345.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-gamma 1
Short name:
EF-1-gamma 1
Alternative name(s):
Calcium and membrane-binding protein 1
Calcium phospholipid-binding protein
Short name:
CPBP
Eukaryotic elongation factor 1Bgamma 1
Short name:
eEF1Bgamma 1
Translation elongation factor 1B gamma 1
Gene namesi
Name:CAM1
Synonyms:TEF3
Ordered Locus Names:YPL048W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL048W.
SGDiS000005969. CAM1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytoplasmic stress granule Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002088312 – 415Elongation factor 1-gamma 1Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei32PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP29547.
PRIDEiP29547.

PTM databases

iPTMnetiP29547.

Interactioni

Subunit structurei

The eukaryotic elongation factor 1 complex (eEF1) is probably a heterohexamer. Two trimeric complexes, each composed of eEF1A (TEF1 or TEF2), eEF1Balpha (EFB1) and eEF1Bgamma (CAM1 or TEF4), are probably dimerized via the eF1Bgamma subunits. The eEF1B subcomplex with the GEF activity is formed of eEF1Balpha and eEF1Bgamma. CAM1 interacts with EFB1. Component of a complex bound to MXR1 promoter region.1 Publication

Protein-protein interaction databases

BioGridi36132. 56 interactors.
DIPiDIP-6813N.
IntActiP29547. 11 interactors.
MINTiMINT-644858.

Structurei

Secondary structure

1415
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Helixi12 – 24Combined sources13
Beta strandi29 – 31Combined sources3
Helixi33 – 35Combined sources3
Helixi37 – 43Combined sources7
Beta strandi49 – 53Combined sources5
Helixi55 – 57Combined sources3
Beta strandi59 – 62Combined sources4
Helixi63 – 73Combined sources11
Helixi77 – 83Combined sources7
Helixi90 – 104Combined sources15
Turni105 – 108Combined sources4
Helixi109 – 113Combined sources5
Helixi115 – 118Combined sources4
Helixi126 – 147Combined sources22
Beta strandi151 – 157Combined sources7
Helixi160 – 175Combined sources16
Helixi179 – 184Combined sources6
Helixi186 – 197Combined sources12
Turni199 – 201Combined sources3
Helixi202 – 204Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NHYX-ray3.00A1-219[»]
ProteinModelPortaliP29547.
SMRiP29547.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29547.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 78GST N-terminalAdd BLAST77
Domaini89 – 215GST C-terminalAdd BLAST127
Domaini254 – 415EF-1-gamma C-terminalPROSITE-ProRule annotationAdd BLAST162

Domaini

Ths GST-like domain mediates the interaction to eEFB1 and may be responsible for dimerization of the eEF1 complex.

Sequence similaritiesi

Contains 1 EF-1-gamma C-terminal domain.PROSITE-ProRule annotation
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

GeneTreeiENSGT00390000007552.
HOGENOMiHOG000201196.
InParanoidiP29547.
KOiK03233.
OMAiYVEAQWS.
OrthoDBiEOG092C37XH.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.30.70.1010. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
IPR001662. Transl_elong_EF1_G_con.
[Graphical view]
PfamiPF00647. EF1G. 1 hit.
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SMARTiSM01183. EF1G. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF89942. SSF89942. 1 hit.
PROSITEiPS50040. EF1G_C. 1 hit.
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQGTLYANF RIRTWVPRGL VKALKLDVKV VTPDAAAEQF ARDFPLKKVP
60 70 80 90 100
AFVGPKGYKL TEAMAINYYL VKLSQDDKMK TQLLGADDDL NAQAQIIRWQ
110 120 130 140 150
SLANSDLCIQ IANTIVPLKG GAPYNKKSVD SAMDAVDKIV DIFENRLKNY
160 170 180 190 200
TYLATENISL ADLVAASIFT RYFESLFGTE WRAQHPAIVR WFNTVRASPF
210 220 230 240 250
LKDEYKDFKF ADKPLSPPQK KKEKKAPAAA PAASKKKEEA KPAATETETS
260 270 280 290 300
SKKPKHPLEL LGKSTFVLDD WKRKYSNEDT RPVALPWFWE HYNPEEYSLW
310 320 330 340 350
KVTYKYNDEL TLTFMSNNLV GGFFNRLSAS TKYMFGCLVV YGENNNNGIV
360 370 380 390 400
GAVMVRGQDY VPAFDVAPDW ESYDYAKLDP TNDDDKEFIN NMWAWDKPVS
410
VNGEPKEIVD GKVLK
Length:415
Mass (Da):47,088
Last modified:October 1, 1996 - v2
Checksum:i17BCE60FFB7B2890
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191W → C in AAA16892 (PubMed:8247005).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01879 Unassigned DNA. Translation: AAA16892.1.
X67917 Genomic DNA. Translation: CAA48116.1.
U44030 Genomic DNA. Translation: AAB68173.1.
BK006949 Genomic DNA. Translation: DAA11382.1.
PIRiS29345.
RefSeqiNP_015277.1. NM_001183862.1.

Genome annotation databases

EnsemblFungiiYPL048W; YPL048W; YPL048W.
GeneIDi856059.
KEGGisce:YPL048W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01879 Unassigned DNA. Translation: AAA16892.1.
X67917 Genomic DNA. Translation: CAA48116.1.
U44030 Genomic DNA. Translation: AAB68173.1.
BK006949 Genomic DNA. Translation: DAA11382.1.
PIRiS29345.
RefSeqiNP_015277.1. NM_001183862.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NHYX-ray3.00A1-219[»]
ProteinModelPortaliP29547.
SMRiP29547.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36132. 56 interactors.
DIPiDIP-6813N.
IntActiP29547. 11 interactors.
MINTiMINT-644858.

PTM databases

iPTMnetiP29547.

Proteomic databases

MaxQBiP29547.
PRIDEiP29547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL048W; YPL048W; YPL048W.
GeneIDi856059.
KEGGisce:YPL048W.

Organism-specific databases

EuPathDBiFungiDB:YPL048W.
SGDiS000005969. CAM1.

Phylogenomic databases

GeneTreeiENSGT00390000007552.
HOGENOMiHOG000201196.
InParanoidiP29547.
KOiK03233.
OMAiYVEAQWS.
OrthoDBiEOG092C37XH.

Enzyme and pathway databases

UniPathwayiUPA00345.
BioCyciYEAST:G3O-33961-MONOMER.
ReactomeiR-SCE-156842. Eukaryotic Translation Elongation.

Miscellaneous databases

EvolutionaryTraceiP29547.
PROiP29547.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.30.70.1010. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
IPR001662. Transl_elong_EF1_G_con.
[Graphical view]
PfamiPF00647. EF1G. 1 hit.
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SMARTiSM01183. EF1G. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF89942. SSF89942. 1 hit.
PROSITEiPS50040. EF1G_C. 1 hit.
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1G1_YEAST
AccessioniPrimary (citable) accession number: P29547
Secondary accession number(s): D6W3W6, Q9URC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 60865 molecules/cell in log phase SD medium.1 Publication
There are 2 isoforms for eEF1Bgamma in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.