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P29538

- MTH1_HAEPA

UniProt

P29538 - MTH1_HAEPA

Protein

Modification methylase HpaI

Gene

hpaIM

Organism
Haemophilus parainfluenzae
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3431. M.HpaI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HpaI (EC:2.1.1.72)
    Short name:
    M.HpaI
    Alternative name(s):
    Adenine-specific methyltransferase HpaI
    Gene namesi
    Name:hpaIM
    OrganismiHaemophilus parainfluenzae
    Taxonomic identifieri729 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314Modification methylase HpaIPRO_0000087965Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP29538.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00508. S21N4MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29538-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQRLICSNA IKALKNLEEN SIDLIITDPP YNLGKDYGTT DDNLNFNKYL    50
    EFSHEWLEEC YRVLKPHGTI YIFMGMKYIS YIYKILEQDL GMYFNSWITW 100
    YYTQGIGKTR GYSPRHDDIL MFTKHPKKFT FNLDRIRVPQ KYYRSVNNMR 150
    GANPSNVWEF SHVHYCNKNR KPHPTQKPEA LYERMILASS NEGDIVLDPF 200
    VGSGTLNFVC KHLNRSGIGI DINKEYIEMA KERLDSEFNG FDSIDERMKR 250
    CPNDLSDPVI RKQYIINHIN WFLKNHENAR EEFLNEVKTK YYKKMTQEEK 300
    RLLDKENNLS FDFS 314
    Length:314
    Mass (Da):37,393
    Last modified:April 1, 1993 - v1
    Checksum:iF72298845BF2778B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10668 Genomic DNA. Translation: BAA01519.1.
    PIRiS28681.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10668 Genomic DNA. Translation: BAA01519.1 .
    PIRi S28681.

    3D structure databases

    ProteinModelPortali P29538.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3431. M.HpaI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00508. S21N4MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the HpaI restriction-modification genes."
      Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.
      Nucleic Acids Res. 20:705-709(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49669.

    Entry informationi

    Entry nameiMTH1_HAEPA
    AccessioniPrimary (citable) accession number: P29538
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3