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P29538 (MTH1_HAEPA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HpaI

Short name=M.HpaI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase HpaI
Gene names
Name:hpaIM
OrganismHaemophilus parainfluenzae
Taxonomic identifier729 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Modification methylase HpaI
PRO_0000087965

Sequences

Sequence LengthMass (Da)Tools
P29538 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: F72298845BF2778B

FASTA31437,393
        10         20         30         40         50         60 
MDQRLICSNA IKALKNLEEN SIDLIITDPP YNLGKDYGTT DDNLNFNKYL EFSHEWLEEC 

        70         80         90        100        110        120 
YRVLKPHGTI YIFMGMKYIS YIYKILEQDL GMYFNSWITW YYTQGIGKTR GYSPRHDDIL 

       130        140        150        160        170        180 
MFTKHPKKFT FNLDRIRVPQ KYYRSVNNMR GANPSNVWEF SHVHYCNKNR KPHPTQKPEA 

       190        200        210        220        230        240 
LYERMILASS NEGDIVLDPF VGSGTLNFVC KHLNRSGIGI DINKEYIEMA KERLDSEFNG 

       250        260        270        280        290        300 
FDSIDERMKR CPNDLSDPVI RKQYIINHIN WFLKNHENAR EEFLNEVKTK YYKKMTQEEK 

       310 
RLLDKENNLS FDFS 

« Hide

References

[1]"Cloning and expression of the HpaI restriction-modification genes."
Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.
Nucleic Acids Res. 20:705-709(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49669.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10668 Genomic DNA. Translation: BAA01519.1.
PIRS28681.

3D structure databases

ProteinModelPortalP29538.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3431. M.HpaI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00508. S21N4MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTH1_HAEPA
AccessionPrimary (citable) accession number: P29538
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries