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P29538

- MTH1_HAEPA

UniProt

P29538 - MTH1_HAEPA

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Protein

Modification methylase HpaI

Gene

hpaIM

Organism
Haemophilus parainfluenzae
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3431. M.HpaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HpaI (EC:2.1.1.72)
Short name:
M.HpaI
Alternative name(s):
Adenine-specific methyltransferase HpaI
Gene namesi
Name:hpaIM
OrganismiHaemophilus parainfluenzae
Taxonomic identifieri729 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Modification methylase HpaIPRO_0000087965Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP29538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29538-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDQRLICSNA IKALKNLEEN SIDLIITDPP YNLGKDYGTT DDNLNFNKYL
60 70 80 90 100
EFSHEWLEEC YRVLKPHGTI YIFMGMKYIS YIYKILEQDL GMYFNSWITW
110 120 130 140 150
YYTQGIGKTR GYSPRHDDIL MFTKHPKKFT FNLDRIRVPQ KYYRSVNNMR
160 170 180 190 200
GANPSNVWEF SHVHYCNKNR KPHPTQKPEA LYERMILASS NEGDIVLDPF
210 220 230 240 250
VGSGTLNFVC KHLNRSGIGI DINKEYIEMA KERLDSEFNG FDSIDERMKR
260 270 280 290 300
CPNDLSDPVI RKQYIINHIN WFLKNHENAR EEFLNEVKTK YYKKMTQEEK
310
RLLDKENNLS FDFS
Length:314
Mass (Da):37,393
Last modified:April 1, 1993 - v1
Checksum:iF72298845BF2778B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10668 Genomic DNA. Translation: BAA01519.1.
PIRiS28681.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10668 Genomic DNA. Translation: BAA01519.1 .
PIRi S28681.

3D structure databases

ProteinModelPortali P29538.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3431. M.HpaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00508. S21N4MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the HpaI restriction-modification genes."
    Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.
    Nucleic Acids Res. 20:705-709(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49669.

Entry informationi

Entry nameiMTH1_HAEPA
AccessioniPrimary (citable) accession number: P29538
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 1, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3