Reviewed, UniProtKB/Swiss-Prot P29538 (MTH1_HAEPA)

Names and origin

Protein names

Recommended name:
    Modification methylase HpaI
      Short name=M.HpaI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase HpaI

Gene names

Name:

hpaIM

Organism

Haemophilus parainfluenzae

Taxonomic identifier

729 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Modification methylase HpaI		PRO_0000087965

Sequences

	Sequence		Length	Mass (Da)
	P29538-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: F72298845BF2778B	FASTA	314	37,393
	10 20 30 40 50 60 MDQRLICSNA IKALKNLEEN SIDLIITDPP YNLGKDYGTT DDNLNFNKYL EFSHEWLEEC 70 80 90 100 110 120 YRVLKPHGTI YIFMGMKYIS YIYKILEQDL GMYFNSWITW YYTQGIGKTR GYSPRHDDIL 130 140 150 160 170 180 MFTKHPKKFT FNLDRIRVPQ KYYRSVNNMR GANPSNVWEF SHVHYCNKNR KPHPTQKPEA 190 200 210 220 230 240 LYERMILASS NEGDIVLDPF VGSGTLNFVC KHLNRSGIGI DINKEYIEMA KERLDSEFNG 250 260 270 280 290 300 FDSIDERMKR CPNDLSDPVI RKQYIINHIN WFLKNHENAR EEFLNEVKTK YYKKMTQEEK 310 RLLDKENNLS FDFS

References

[1]	"Cloning and expression of the HpaI restriction-modification genes." Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I. Nucleic Acids Res. 20:705-709(1992) [PubMed: 1542567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49669.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10668 Genomic DNA. Translation: BAA01519.1.
PIR	S28681.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3431. M.HpaI.
Enzyme and pathway databases
BRENDA	2.1.1.72. 96187.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. MeTrfase_CN4. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTH1_HAEPA

Accession

Primary (citable) accession number: P29538

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	September 22, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)