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P29537 (T2H1_HAEPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme HpaI

Short name=R.HpaI
EC=3.1.21.4
Alternative name(s):
Endonuclease HpaI
Type II restriction enzyme HpaI
Gene names
Name:hpaIR
OrganismHaemophilus parainfluenzae
Taxonomic identifier729 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GTTAAC and cleaves after T-3.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Type-2 restriction enzyme HpaI
PRO_0000077324

Sequences

Sequence LengthMass (Da)Tools
P29537 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: C196D22A771B9CED

FASTA25429,629
        10         20         30         40         50         60 
MKYEEINFKV PVESPYYPNY SQCVIERIYS ILRNQKDMGD DRIIINTNLK KGLPLENINK 

        70         80         90        100        110        120 
IAGPMIEAWA EEVFSGIRDN RDNQYNLINV EAQERLGISD IILQFQVNNN VITGNVDVKA 

       130        140        150        160        170        180 
TSNDIPDSGK SPNITSFSRI RTAYVKDPNF IFIILSIKHS VYVKRNEYTN LMDGIMQIID 

       190        200        210        220        230        240 
FNVYDLKYIS DSDISYNPAL GTGQIQIKDI HYVSSQKRTT WQMCQLLDLK YLRSKKRTIE 

       250 
QFYNEAKRNK WIKD 

« Hide

References

[1]"Cloning and expression of the HpaI restriction-modification genes."
Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.
Nucleic Acids Res. 20:705-709(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22.
Strain: ATCC 49669.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10668 Genomic DNA. Translation: BAA01518.1.
PIRS28680.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1158. HpaI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2H1_HAEPA
AccessionPrimary (citable) accession number: P29537
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries