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P29537

- T2H1_HAEPA

UniProt

P29537 - T2H1_HAEPA

Protein

Type-2 restriction enzyme HpaI

Gene

hpaIR

Organism
Haemophilus parainfluenzae
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (16 Apr 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GTTAAC and cleaves after T-3.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1158. HpaI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme HpaI (EC:3.1.21.4)
    Short name:
    R.HpaI
    Alternative name(s):
    Endonuclease HpaI
    Type II restriction enzyme HpaI
    Gene namesi
    Name:hpaIR
    OrganismiHaemophilus parainfluenzae
    Taxonomic identifieri729 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 254254Type-2 restriction enzyme HpaIPRO_0000077324Add
    BLAST

    Sequencei

    Sequence statusi: Complete.

    P29537-1 [UniParc]FASTAAdd to Basket

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    MKYEEINFKV PVESPYYPNY SQCVIERIYS ILRNQKDMGD DRIIINTNLK    50
    KGLPLENINK IAGPMIEAWA EEVFSGIRDN RDNQYNLINV EAQERLGISD 100
    IILQFQVNNN VITGNVDVKA TSNDIPDSGK SPNITSFSRI RTAYVKDPNF 150
    IFIILSIKHS VYVKRNEYTN LMDGIMQIID FNVYDLKYIS DSDISYNPAL 200
    GTGQIQIKDI HYVSSQKRTT WQMCQLLDLK YLRSKKRTIE QFYNEAKRNK 250
    WIKD 254
    Length:254
    Mass (Da):29,629
    Last modified:April 1, 1993 - v1
    Checksum:iC196D22A771B9CED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10668 Genomic DNA. Translation: BAA01518.1.
    PIRiS28680.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10668 Genomic DNA. Translation: BAA01518.1 .
    PIRi S28680.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1158. HpaI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the HpaI restriction-modification genes."
      Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.
      Nucleic Acids Res. 20:705-709(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22.
      Strain: ATCC 49669.

    Entry informationi

    Entry nameiT2H1_HAEPA
    AccessioniPrimary (citable) accession number: P29537
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: April 16, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3