P29537 (T2H1_HAEPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme HpaI
Short name=R.HpaI
EC=3.1.21.4

Alternative name(s):
Endonuclease HpaI
Type II restriction enzyme HpaI

Gene names

Name:

hpaIR

Organism

Haemophilus parainfluenzae

Taxonomic identifier

729 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes the double-stranded sequence GTTAAC and cleaves after T-3.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Type-2 restriction enzyme HpaI		PRO_0000077324

Sequences

	Sequence		Length	Mass (Da)
	P29537 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: C196D22A771B9CED	FASTA	254	29,629
	10 20 30 40 50 60 MKYEEINFKV PVESPYYPNY SQCVIERIYS ILRNQKDMGD DRIIINTNLK KGLPLENINK 70 80 90 100 110 120 IAGPMIEAWA EEVFSGIRDN RDNQYNLINV EAQERLGISD IILQFQVNNN VITGNVDVKA 130 140 150 160 170 180 TSNDIPDSGK SPNITSFSRI RTAYVKDPNF IFIILSIKHS VYVKRNEYTN LMDGIMQIID 190 200 210 220 230 240 FNVYDLKYIS DSDISYNPAL GTGQIQIKDI HYVSSQKRTT WQMCQLLDLK YLRSKKRTIE 250 QFYNEAKRNK WIKD

References

[1]	"Cloning and expression of the HpaI restriction-modification genes." Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I. Nucleic Acids Res. 20:705-709(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22. Strain: ATCC 49669.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10668 Genomic DNA. Translation: BAA01518.1.
PIR	S28680.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1158. HpaI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2H1_HAEPA

Accession

Primary (citable) accession number: P29537

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program