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P29537

- T2H1_HAEPA

UniProt

P29537 - T2H1_HAEPA

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Protein

Type-2 restriction enzyme HpaI

Gene

hpaIR

Organism
Haemophilus parainfluenzae
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GTTAAC and cleaves after T-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1158. HpaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HpaI (EC:3.1.21.4)
Short name:
R.HpaI
Alternative name(s):
Endonuclease HpaI
Type II restriction enzyme HpaI
Gene namesi
Name:hpaIR
OrganismiHaemophilus parainfluenzae
Taxonomic identifieri729 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Type-2 restriction enzyme HpaIPRO_0000077324Add
BLAST

Sequencei

Sequence statusi: Complete.

P29537-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MKYEEINFKV PVESPYYPNY SQCVIERIYS ILRNQKDMGD DRIIINTNLK
60 70 80 90 100
KGLPLENINK IAGPMIEAWA EEVFSGIRDN RDNQYNLINV EAQERLGISD
110 120 130 140 150
IILQFQVNNN VITGNVDVKA TSNDIPDSGK SPNITSFSRI RTAYVKDPNF
160 170 180 190 200
IFIILSIKHS VYVKRNEYTN LMDGIMQIID FNVYDLKYIS DSDISYNPAL
210 220 230 240 250
GTGQIQIKDI HYVSSQKRTT WQMCQLLDLK YLRSKKRTIE QFYNEAKRNK

WIKD
Length:254
Mass (Da):29,629
Last modified:April 1, 1993 - v1
Checksum:iC196D22A771B9CED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10668 Genomic DNA. Translation: BAA01518.1.
PIRiS28680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10668 Genomic DNA. Translation: BAA01518.1 .
PIRi S28680.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1158. HpaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the HpaI restriction-modification genes."
    Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.
    Nucleic Acids Res. 20:705-709(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22.
    Strain: ATCC 49669.

Entry informationi

Entry nameiT2H1_HAEPA
AccessioniPrimary (citable) accession number: P29537
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3