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P29537

- T2H1_HAEPA

UniProt

P29537 - T2H1_HAEPA

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Protein
Type-2 restriction enzyme HpaI
Gene
hpaIR
Organism
Haemophilus parainfluenzae
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GTTAAC and cleaves after T-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1158. HpaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HpaI (EC:3.1.21.4)
Short name:
R.HpaI
Alternative name(s):
Endonuclease HpaI
Type II restriction enzyme HpaI
Gene namesi
Name:hpaIR
OrganismiHaemophilus parainfluenzae
Taxonomic identifieri729 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Type-2 restriction enzyme HpaI
PRO_0000077324Add
BLAST

Sequencei

Sequence statusi: Complete.

P29537-1 [UniParc]FASTAAdd to Basket

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MKYEEINFKV PVESPYYPNY SQCVIERIYS ILRNQKDMGD DRIIINTNLK    50
KGLPLENINK IAGPMIEAWA EEVFSGIRDN RDNQYNLINV EAQERLGISD 100
IILQFQVNNN VITGNVDVKA TSNDIPDSGK SPNITSFSRI RTAYVKDPNF 150
IFIILSIKHS VYVKRNEYTN LMDGIMQIID FNVYDLKYIS DSDISYNPAL 200
GTGQIQIKDI HYVSSQKRTT WQMCQLLDLK YLRSKKRTIE QFYNEAKRNK 250
WIKD 254
Length:254
Mass (Da):29,629
Last modified:April 1, 1993 - v1
Checksum:iC196D22A771B9CED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10668 Genomic DNA. Translation: BAA01518.1.
PIRiS28680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10668 Genomic DNA. Translation: BAA01518.1 .
PIRi S28680.

3D structure databases

ModBasei Search...

Protein family/group databases

REBASEi 1158. HpaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the HpaI restriction-modification genes."
    Ito H., Shimato H., Sadaoka A., Kotani H., Kimizuka F., Kato I.
    Nucleic Acids Res. 20:705-709(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-22.
    Strain: ATCC 49669.

Entry informationi

Entry nameiT2H1_HAEPA
AccessioniPrimary (citable) accession number: P29537
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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