Reviewed, UniProtKB/Swiss-Prot P29535 (1A14_SOLLC)

Names and origin

Protein names

Recommended name:
    1-aminocyclopropane-1-carboxylate synthase 4
      Short name=ACC synthase 4
    EC=4.4.1.14
Alternative name(s):
    Le-ACS4
      Short name=ACS-4
    S-adenosyl-L-methionine methylthioadenosine-lyase 4

Gene names

Name:	ACS4
Synonyms:	ACC4, BTAS4, PCVV4B

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of 1-aminocyclopropane-1-carboxylate, a direct precursor of ethylene in higher plants.
Catalytic activity	S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.
Cofactor	Pyridoxal phosphate.
Pathway	Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 1/2.
Subunit structure	Homodimer.
Induction	Associated with fruit ripening, but unresponsive to auxin treatment in vegetative tissue. Ref.5
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Ethylene biosynthesis Fruit ripening
Ligand	Pyridoxal phosphate S-adenosyl-L-methionine
Molecular function	Lyase
Gene Ontology (GO)
Biological process	ethylene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	1-aminocyclopropane-1-carboxylate synthase 4		PRO_0000123914
Amino acid modifications
	Modified residue	282	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	15	1	V → A in CAA41857. Ref.2
	Sequence conflict	82	1	T → A in AAA81581. Ref.4
	Sequence conflict	253	1	S → P Ref.2
	Sequence conflict	253	1	S → P Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P29535-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 9BC7D97BD64CB044	FASTA	476	53,537
	10 20 30 40 50 60 MDLETSEISN YKSSVVLSKL ASNEQHGENS PYFDGWKAYD NDPFHLVNNL NGVIQMGLAE 70 80 90 100 110 120 NQLSVDLIEE WIKRNPKASI CTNDGIESFR RIANFQDYHG LPEFTNAIAK FMEKTRGGKV 130 140 150 160 170 180 KFDAKRVVMA GGATGANETL ILCLADPGDA FLVPTPYYPG FNRDLRWRSG VQLLPISCKS 190 200 210 220 230 240 CNNFKITIEA IEEAYEKGQQ ANVKIKGLIL TNPCNPLGTI LDRDTLKKIS TFTNEHNIHL 250 260 270 280 290 300 VCDEIYAATV FNSPKFVSIA EIINEDNCIN KDLVHIVSSL SKDLGFPGFR VGIVYSFNDD 310 320 330 340 350 360 VVNCARKMSS FGLVSTQTQH LLAFMLSDDE FVEEFLIESA KRLRERYEKF TRGLEEIGIK 370 380 390 400 410 420 CLESNAGVYC WMDLRSLLKE ATLDAEMSLW KLIINEVKLN VSPGSSFNCS EVGWFRVCFA 430 440 450 460 470 NIDDQTMEIA LARIRMFMDA YNNVNKNGVM KNKHNGRGTT YDLTPQMGST MKMLLA

References

[1]	"LE-ACS4, a fruit ripening and wound-induced 1-aminocyclopropane-1-carboxylate synthase gene of tomato (Lycopersicon esculentum). Expression in Escherichia coli, structural characterization, expression characteristics, and phylogenetic analysis." Lincoln J.E., Campbell A.D., Oetiker J., Rottmann W.H., Oeller P.W., Shen N.F., Theologis A. J. Biol. Chem. 268:19422-19430(1993) [PubMed: 8366090] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Caruso. Tissue: Etiolated hypocotyl.
[2]	"1-aminocyclopropane-1-carboxylate synthase in tomato is encoded by a multigene family whose transcription is induced during fruit and floral senescence." Rottmann W.H., Peter G.F., Oeller P.W., Keller J.A., Shen N.F., Nagy B.P., Taylor L.P., Campbell A.D., Theologis A. J. Mol. Biol. 222:937-961(1991) [PubMed: 1762159] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Etiolated hypocotyl.
[3]	"Differential expression of two genes for 1-aminocyclopropane-1-carboxylate synthase in tomato fruits." Olson D.C., White J.A., Edelman L., Harkins R.N., Kende H. Proc. Natl. Acad. Sci. U.S.A. 88:5340-5344(1991) [PubMed: 1711229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1-carboxylate synthase in tomato." van der Straeten D., van Wiemeersch L., Goodman H.M., van Montagu M. Proc. Natl. Acad. Sci. U.S.A. 87:4859-4863(1990) [PubMed: 2191304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-159. Strain: cv. Orlando. Tissue: Fruit.
[5]	"Differential accumulation of transcripts for four tomato 1-aminocyclopropane-1-carboxylate synthase homologs under various conditions." Yip W.K., Moore T., Yang S.F. Proc. Natl. Acad. Sci. U.S.A. 89:2475-2479(1992) [PubMed: 1549612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 235-307, INDUCTION. Tissue: Pericarp.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M88487 Unassigned DNA. Translation: AAA03164.1. X59146 mRNA. Translation: CAA41857.1. M63490 mRNA. Translation: AAA34131.1. M38705 mRNA. Translation: AAA81581.1. M83329 mRNA. Translation: AAA68623.1.
PIR	S19679.
UniGene	Les.21209 Les.21920
3D structure databases
HSSP	HSSP built from PDB template 1IAX based on UniProtKB P18485.
SMR	P29535. Positions 21-438.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.4.1.14. 281054.
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

1A14_SOLLC

Accession

Primary (citable) accession number: P29535
Secondary accession number(s): Q42894

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	September 22, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)