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Reviewed, UniProtKB/Swiss-Prot P29533 (VCAM1_MOUSE)

Last modified July 13, 2010. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
Vascular cell adhesion protein 1

Short name=V-CAM 1
Short name=VCAM-1
Alternative name(s):
CD_antigen=CD106
Gene names
Name:Vcam1
Synonyms:Vcam-1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus
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Protein attributesHide

Sequence length739 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Function

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Subunit structure

Binds to ECMV-D capsid proteins and acts as a receptor for this virus. Ref.9

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Isoform 2: Cell membrane; Lipid-anchorGPI-anchor.

Tissue specificity

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. Ref.10

Post-translational modification

The GPI-anchor is located on position 319 of isoform 2.

Sequence similarities

Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

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Alternative productsHide

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P29533-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P29533-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     310-345: EKPFIVDISPGSQVAAQVGDSVVLTCAAIGCDSPSF → DGRMKSQITNGHQLTVHLMFAKSFYFICYLCLYLAL
     346-739: Missing.
Note: GPI-anchored form.
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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Probable
Chain25 – 739715Vascular cell adhesion protein 1
PRO_0000014998

Regions

Topological domain25 – 698674Extracellular Potential
Transmembrane699 – 72022Helical; Potential
Topological domain721 – 73919Cytoplasmic Potential
Domain25 – 11187Ig-like C2-type 1
Domain119 – 21294Ig-like C2-type 2
Domain223 – 30987Ig-like C2-type 3
Domain312 – 39382Ig-like C2-type 4
Domain408 – 50699Ig-like C2-type 5
Domain511 – 59585Ig-like C2-type 6
Domain600 – 68283Ig-like C2-type 7

Amino acid modifications

Glycosylation2251N-linked (GlcNAc...) Ref.11
Glycosylation2641N-linked (GlcNAc...) Ref.11
Glycosylation2731N-linked (GlcNAc...) Ref.11
Glycosylation4241N-linked (GlcNAc...) Potential
Glycosylation5311N-linked (GlcNAc...) Potential
Glycosylation5521N-linked (GlcNAc...) Ref.11
Glycosylation5611N-linked (GlcNAc...) Ref.11
Disulfide bond47 ↔ 95 By similarity
Disulfide bond52 ↔ 99 By similarity
Disulfide bond137 ↔ 195 By similarity
Disulfide bond246 ↔ 291 By similarity
Disulfide bond335 ↔ 383 By similarity
Disulfide bond534 ↔ 579 By similarity

Natural variations

Alternative sequence310 – 34536EKPFI…DSPSF → DGRMKSQITNGHQLTVHLMF AKSFYFICYLCLYLAL in isoform 2.
VSP_002581
Alternative sequence346 – 739394Missing in isoform 2.
VSP_002582

Experimental info

Sequence conflict6931D → N in AAA16921. Ref.3
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SequencesHide

Sequence LengthMass (Da)Tools
Isoform 1 (Long) [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 3D2134C341E5E449

FASTA73981,317
        10         20         30         40         50         60 
MPVKMVAVLG ASTVLWILFA VSQAFKIEIS PEYKTIAQIG DSMALTCSTT GCESPLFSWR 

        70         80         90        100        110        120 
TQIDSPLNAK VRTEGSKSVL TMEPVSFENE HSYLCTATCG SGKLERSIHV DIYSFPKDPE 

       130        140        150        160        170        180 
IQFSGPLEVG KPVTVKCLAP DIYPVYRLEI DLFKGDQLMN RQEFSSEEMT KSLETKSLEV 

       190        200        210        220        230        240 
TFTPVIEDIG KALVCRAKLH IDQIDSTLKE RETVKELQVY ISPRNTTISV HPSTRLQEGG 

       250        260        270        280        290        300 
AVTMTCSSEG LPAPEIFWGR KLDNEVLQLL SGNATLTLIA MRMEDSGVYV CEGVNLIGRD 

       310        320        330        340        350        360 
KAEVELVVQE KPFIVDISPG SQVAAQVGDS VVLTCAAIGC DSPSFSWRTQ TDSPLNGVVR 

       370        380        390        400        410        420 
NEGAKSTLVL SSVGFEDEHS YLCAVTCLQR TLEKRTQVEV YSFPEDPVIK MSGPLVHGRP 

       430        440        450        460        470        480 
VTVNCTVPNV YPFDHLEIEL LKGETTLMKK YFLEEMGIKS LETKILETTF IPTIEDTGKS 

       490        500        510        520        530        540 
LVCLARLHSG EMESEPKQRQ SVQPLYVNVA PKETTIWVSP SPILEEGSPV NLTCSSDGIP 

       550        560        570        580        590        600 
APKILWSRQL NNGELQPLSE NTTLTFMSTK RDDSGIYVCE GINEAGISRK SVELIIQVSP 

       610        620        630        640        650        660 
KDIQLTVFPS KSVKEGDTVI ISCTCGNVPE TWIILKKKAK TGDMVLKSVD GSYTIRQAQL 

       670        680        690        700        710        720 
QDAGIYECES KTEVGSQLRS LTLDVKGKEH NKDYFSPELL ALYCASSLVI PAIGMIVYFA 

       730 
RKANMKGSYS LVEAQKSKV 

« Hide

Isoform 2 (Short).

Checksum: 973A28A199C6EA15
Show »

FASTA34538,372
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ReferencesHide

« Hide 'large scale' references
[1]"Cloning of murine and rat vascular cell adhesion molecule-1."
Hession C., Moy P., Tizard R., Chisholm P., Williams C., Wysk M., Burkly L., Miyake K., Kincade P., Lobb R.
Biochem. Biophys. Res. Commun. 183:163-169(1992) [PubMed: 1371918] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: FVB.
Tissue: Lung.
[2]"Cloning and sequencing of mouse VCAM-1 cDNA."
Araki M., Araki K., Vassalli P.
Gene 126:261-264(1993) [PubMed: 7683304] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
Tissue: Lymph node.
[3]"Structure of the murine VCAM1 gene."
Cybulsky M.I., Allan-Motamed M., Collins T.
Genomics 18:387-391(1993) [PubMed: 7507076] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
Strain: 129.
Tissue: Embryo.
[4]Kumar A.G., Dai Y.X., Kozak C.A., Mims M.P., Gotto A.M. Jr., Ballantyne C.M.
Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 1-693 (ISOFORM 1).
Strain: 129/Sv and NIH Swiss.
[5]"Cloning of an inflammation-specific phosphatidyl inositol-linked form of murine vascular cell adhesion molecule-1."
Moy P., Lobb R., Tizard R., Olson D., Hession C.
J. Biol. Chem. 268:8835-8841(1993) [PubMed: 7682556] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: FVB.
Tissue: Lung.
[6]"Murine VCAM-1. Molecular cloning, mapping, and analysis of a truncated form."
Kumar A.G., Dai X.Y., Kozak C.A., Mims M.P., Gotto A.M. Jr., Ballantyne C.M.
J. Immunol. 153:4088-4098(1994) [PubMed: 7523515] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Liver.
[7]"Cytokine induction of an alternatively spliced murine vascular cell adhesion molecule (VCAM) mRNA encoding a glycosylphosphatidylinositol-anchored VCAM protein."
Terry R.W., Kwee L., Levine J.F., Labow M.A.
Proc. Natl. Acad. Sci. U.S.A. 90:5919-5923(1993) [PubMed: 7687058] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 311-345 (ISOFORM 2).
Strain: FVB/N.
Tissue: Kidney.
[8]Korenaga R., Ando J., Tsuboi H., Kamiya A.
Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 1-21.
Tissue: Endothelial cell.
[9]"VCAM-1 is a receptor for encephalomyocarditis virus on murine vascular endothelial cells."
Huber S.A.
J. Virol. 68:3453-3458(1994) [PubMed: 7514674] [Abstract]
Cited for: INTERACTION WITH ECMV-D CAPSID PROTEINS.
[10]"A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture."
Miyake K., Medina K., Ishihara K., Kimoto M., Auerbach R., Kincade P.W.
J. Cell Biol. 114:557-565(1991) [PubMed: 1713592] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-32, TISSUE SPECIFICITY.
[11]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed: 19656770] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-225; ASN-264; ASN-273; ASN-552 AND ASN-561, MASS SPECTROMETRY.
Tissue: Myoblast.
+Additional computationally mapped references.
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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ
M84487 mRNA. Translation: AAA40545.1.
X67783 mRNA. Translation: CAA47989.1.
L22355 expand/collapse EMBL AC list , L22301, L22349, L22350, L22351, L22352, L22353, L22354 Genomic DNA. Translation: AAA16921.1.
L22350, L22301, L22349 Genomic DNA. Translation: AAA16920.1.
U12878 Genomic DNA. Translation: AAB60659.1. Sequence problems.
U12879 Genomic DNA. Translation: AAB60660.1. Sequence problems.
U12880 Genomic DNA. Translation: AAB60661.1. Sequence problems.
U12874 Genomic DNA. Translation: AAB60662.1. Sequence problems.
U12871 Genomic DNA. Translation: AAB60663.1. Sequence problems.
U12883 Genomic DNA. Translation: AAB60664.1. Sequence problems.
U12881 Genomic DNA. Translation: AAA80010.1. Sequence problems.
U12882 Genomic DNA. Translation: AAA80011.1. Sequence problems.
U12875 Genomic DNA. Translation: AAA80012.1. Sequence problems.
U12872 Genomic DNA. Translation: AAA80013.1. Sequence problems.
U12876 Genomic DNA. Translation: AAA80014.1. Sequence problems.
U12873 Genomic DNA. Translation: AAA80015.1. Sequence problems.
U12877 Genomic DNA. Translation: AAA80016.1. Sequence problems.
L08431 mRNA. Translation: AAA40546.1.
U12884 mRNA. Translation: AAA64832.1.
L12541 mRNA. Translation: AAC37607.1.
U42327 Genomic DNA. Translation: AAB88576.1.
IPIIPI00126834.
IPI00264750.
PIRA46052. B48919.
JN0581.
RefSeqNP_035823.3.
UniGeneMm.440909
Mm.76649

3D structure databases

SMRP29533. Positions 25-223, 35-386, 235-685.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-29097N.
STRINGP29533.

PTM databases

PhosphoSiteP29533.

Proteomic databases

PRIDEP29533.

Genome annotation databases

EnsemblENSMUST00000029574; ENSMUSP00000029574; ENSMUSG00000027962; Mus musculus. [Genome view]
ENSMUST00000106493; ENSMUSP00000102102; ENSMUSG00000027962; Mus musculus. [Genome view]
GeneID22329.
KEGGmmu:22329.

Organism-specific databases

CTD22329.
MGIMGI:98926. Vcam1.

Phylogenomic databases

eggNOGroNOG04595.
HOGENOMHBG126178.
HOVERGENHBG053965.
InParanoidP29533.

Gene expression databases

ArrayExpressP29533.
BgeeP29533.
CleanExMM_VCAM1.
GenevestigatorP29533.
GermOnlineENSMUSG00000027962. Mus musculus.

Family and domain databases

InterProIPR003987. ICAM_VCAM_N.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR003989. VCAM-1.
[Graphical view]
Gene3DG3DSA:2.60.40.10. Ig-like_fold. 7 hits.
PfamPF05790. C2-set. 2 hits.
PF07679. I-set. 5 hits.
[Graphical view]
PRINTSPR01472. ICAMVCAM1.
PR01474. VCAM1.
SMARTSM00409. IG. 2 hits.
SM00408. IGc2. 3 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...
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Entry informationHide

Entry nameVCAM1_MOUSE
AccessionPrimary (citable) accession number: P29533
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 13, 2010
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
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Relevant documentsHide

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents