P29533 (VCAM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vascular cell adhesion protein 1
Short name=V-CAM 1
Short name=VCAM-1

Alternative name(s):
CD_antigen=CD106

Gene names

Name:	Vcam1
Synonyms:	Vcam-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	739 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Subunit structure	Binds to ECMV-D capsid proteins and acts as a receptor for this virus. Ref.9
Subcellular location	Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. Ref.10
Post-translational modification	The GPI-anchor is located on position 319 of isoform 2.
Sequence similarities	Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion Host-virus interaction
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to glucose stimulus Inferred from direct assay PubMed 17670746. Source: MGI chorio-allantoic fusion Inferred from mutant phenotype PubMed 7530222PubMed 7539357. Source: MGI heterophilic cell-cell adhesion Inferred from mutant phenotype PubMed 8145052. Source: MGI leukocyte cell-cell adhesion Inferred from mutant phenotype PubMed 8562500. Source: MGI virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW visceral serous pericardium development Traceable author statement PubMed 20299672. Source: DFLAT
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW cell periphery Inferred from direct assay PubMed 21464233. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P29533-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P29533-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     310-345: EKPFIVDISPGSQVAAQVGDSVVLTCAAIGCDSPSF → DGRMKSQITNGHQLTVHLMFAKSFYFICYLCLYLAL
     346-739: Missing.

Note: GPI-anchor amidated asparagine on Asn-319.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Probable
	Chain	25 – 739	715	Vascular cell adhesion protein 1		PRO_0000014998
Regions
	Topological domain	25 – 698	674	Extracellular Potential
	Transmembrane	699 – 720	22	Helical; Potential
	Topological domain	721 – 739	19	Cytoplasmic Potential
	Domain	25 – 111	87	Ig-like C2-type 1
	Domain	119 – 212	94	Ig-like C2-type 2
	Domain	223 – 309	87	Ig-like C2-type 3
	Domain	312 – 393	82	Ig-like C2-type 4
	Domain	408 – 506	99	Ig-like C2-type 5
	Domain	511 – 595	85	Ig-like C2-type 6
	Domain	600 – 682	83	Ig-like C2-type 7
Amino acid modifications
	Glycosylation	225	1	N-linked (GlcNAc...) Ref.11
	Glycosylation	264	1	N-linked (GlcNAc...) Ref.11
	Glycosylation	273	1	N-linked (GlcNAc...) Ref.11
	Glycosylation	424	1	N-linked (GlcNAc...) Potential
	Glycosylation	531	1	N-linked (GlcNAc...) Potential
	Glycosylation	552	1	N-linked (GlcNAc...) Ref.11
	Glycosylation	561	1	N-linked (GlcNAc...) Ref.11
	Disulfide bond	47 ↔ 95		By similarity
	Disulfide bond	52 ↔ 99		By similarity
	Disulfide bond	137 ↔ 195		By similarity
	Disulfide bond	246 ↔ 291		By similarity
	Disulfide bond	335 ↔ 383		By similarity
	Disulfide bond	534 ↔ 579		By similarity
Natural variations
	Alternative sequence	310 – 345	36	EKPFI…DSPSF → DGRMKSQITNGHQLTVHLMF AKSFYFICYLCLYLAL in isoform 2.		VSP_002581
	Alternative sequence	346 – 739	394	Missing in isoform 2.		VSP_002582
Experimental info
	Sequence conflict	693	1	D → N in AAA16921. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 3D2134C341E5E449	FASTA	739	81,317
	10 20 30 40 50 60 MPVKMVAVLG ASTVLWILFA VSQAFKIEIS PEYKTIAQIG DSMALTCSTT GCESPLFSWR 70 80 90 100 110 120 TQIDSPLNAK VRTEGSKSVL TMEPVSFENE HSYLCTATCG SGKLERSIHV DIYSFPKDPE 130 140 150 160 170 180 IQFSGPLEVG KPVTVKCLAP DIYPVYRLEI DLFKGDQLMN RQEFSSEEMT KSLETKSLEV 190 200 210 220 230 240 TFTPVIEDIG KALVCRAKLH IDQIDSTLKE RETVKELQVY ISPRNTTISV HPSTRLQEGG 250 260 270 280 290 300 AVTMTCSSEG LPAPEIFWGR KLDNEVLQLL SGNATLTLIA MRMEDSGVYV CEGVNLIGRD 310 320 330 340 350 360 KAEVELVVQE KPFIVDISPG SQVAAQVGDS VVLTCAAIGC DSPSFSWRTQ TDSPLNGVVR 370 380 390 400 410 420 NEGAKSTLVL SSVGFEDEHS YLCAVTCLQR TLEKRTQVEV YSFPEDPVIK MSGPLVHGRP 430 440 450 460 470 480 VTVNCTVPNV YPFDHLEIEL LKGETTLMKK YFLEEMGIKS LETKILETTF IPTIEDTGKS 490 500 510 520 530 540 LVCLARLHSG EMESEPKQRQ SVQPLYVNVA PKETTIWVSP SPILEEGSPV NLTCSSDGIP 550 560 570 580 590 600 APKILWSRQL NNGELQPLSE NTTLTFMSTK RDDSGIYVCE GINEAGISRK SVELIIQVSP 610 620 630 640 650 660 KDIQLTVFPS KSVKEGDTVI ISCTCGNVPE TWIILKKKAK TGDMVLKSVD GSYTIRQAQL 670 680 690 700 710 720 QDAGIYECES KTEVGSQLRS LTLDVKGKEH NKDYFSPELL ALYCASSLVI PAIGMIVYFA 730 RKANMKGSYS LVEAQKSKV
	Isoform 2 (Short) [UniParc]. Checksum: 973A28A199C6EA15	FASTA	345	38,372
	10 20 30 40 50 60 MPVKMVAVLG ASTVLWILFA VSQAFKIEIS PEYKTIAQIG DSMALTCSTT GCESPLFSWR 70 80 90 100 110 120 TQIDSPLNAK VRTEGSKSVL TMEPVSFENE HSYLCTATCG SGKLERSIHV DIYSFPKDPE 130 140 150 160 170 180 IQFSGPLEVG KPVTVKCLAP DIYPVYRLEI DLFKGDQLMN RQEFSSEEMT KSLETKSLEV 190 200 210 220 230 240 TFTPVIEDIG KALVCRAKLH IDQIDSTLKE RETVKELQVY ISPRNTTISV HPSTRLQEGG 250 260 270 280 290 300 AVTMTCSSEG LPAPEIFWGR KLDNEVLQLL SGNATLTLIA MRMEDSGVYV CEGVNLIGRD 310 320 330 340 KAEVELVVQD GRMKSQITNG HQLTVHLMFA KSFYFICYLC LYLAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of murine and rat vascular cell adhesion molecule-1." Hession C., Moy P., Tizard R., Chisholm P., Williams C., Wysk M., Burkly L., Miyake K., Kincade P., Lobb R. Biochem. Biophys. Res. Commun. 183:163-169(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: FVB. Tissue: Lung.
[2]	"Cloning and sequencing of mouse VCAM-1 cDNA." Araki M., Araki K., Vassalli P. Gene 126:261-264(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1). Tissue: Lymph node.
[3]	"Structure of the murine VCAM1 gene." Cybulsky M.I., Allan-Motamed M., Collins T. Genomics 18:387-391(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1). Strain: 129. Tissue: Embryo.
[4]	Kumar A.G., Dai Y.X., Kozak C.A., Mims M.P., Gotto A.M. Jr., Ballantyne C.M. Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-693 (ISOFORM 1). Strain: 129/Sv and NIH Swiss.
[5]	"Cloning of an inflammation-specific phosphatidyl inositol-linked form of murine vascular cell adhesion molecule-1." Moy P., Lobb R., Tizard R., Olson D., Hession C. J. Biol. Chem. 268:8835-8841(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: FVB. Tissue: Lung.
[6]	"Murine VCAM-1. Molecular cloning, mapping, and analysis of a truncated form." Kumar A.G., Dai X.Y., Kozak C.A., Mims M.P., Gotto A.M. Jr., Ballantyne C.M. J. Immunol. 153:4088-4098(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Liver.
[7]	"Cytokine induction of an alternatively spliced murine vascular cell adhesion molecule (VCAM) mRNA encoding a glycosylphosphatidylinositol-anchored VCAM protein." Terry R.W., Kwee L., Levine J.F., Labow M.A. Proc. Natl. Acad. Sci. U.S.A. 90:5919-5923(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 311-345 (ISOFORM 2). Strain: FVB/N. Tissue: Kidney.
[8]	Korenaga R., Ando J., Tsuboi H., Kamiya A. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-21. Tissue: Endothelial cell.
[9]	"VCAM-1 is a receptor for encephalomyocarditis virus on murine vascular endothelial cells." Huber S.A. J. Virol. 68:3453-3458(1994) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ECMV-D CAPSID PROTEINS.
[10]	"A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture." Miyake K., Medina K., Ishihara K., Kimoto M., Auerbach R., Kincade P.W. J. Cell Biol. 114:557-565(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-32, TISSUE SPECIFICITY.
[11]	"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-225; ASN-264; ASN-273; ASN-552 AND ASN-561, MASS SPECTROMETRY. Tissue: Myoblast.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

VCAM-1

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84487 mRNA. Translation: AAA40545.1. X67783 mRNA. Translation: CAA47989.1. L22355 , L22301, L22349, L22350, L22351, L22352, L22353, L22354 Genomic DNA. Translation: AAA16921.1. L22350, L22301, L22349 Genomic DNA. Translation: AAA16920.1. U12878 Genomic DNA. Translation: AAB60659.1. Sequence problems. U12879 Genomic DNA. Translation: AAB60660.1. Sequence problems. U12880 Genomic DNA. Translation: AAB60661.1. Sequence problems. U12874 Genomic DNA. Translation: AAB60662.1. Sequence problems. U12871 Genomic DNA. Translation: AAB60663.1. Sequence problems. U12883 Genomic DNA. Translation: AAB60664.1. Sequence problems. U12881 Genomic DNA. Translation: AAA80010.1. Sequence problems. U12882 Genomic DNA. Translation: AAA80011.1. Sequence problems. U12875 Genomic DNA. Translation: AAA80012.1. Sequence problems. U12872 Genomic DNA. Translation: AAA80013.1. Sequence problems. U12876 Genomic DNA. Translation: AAA80014.1. Sequence problems. U12873 Genomic DNA. Translation: AAA80015.1. Sequence problems. U12877 Genomic DNA. Translation: AAA80016.1. Sequence problems. L08431 mRNA. Translation: AAA40546.1. U12884 mRNA. Translation: AAA64832.1. L12541 mRNA. Translation: AAC37607.1. U42327 Genomic DNA. Translation: AAB88576.1.
IPI	IPI00126834. IPI00264750.
PIR	A46052. B48919. JN0581.
RefSeq	NP_035823.3. NM_011693.3.
UniGene	Mm.440909. Mm.76649.
3D structure databases
ProteinModelPortal	P29533.
SMR	P29533. Positions 25-686.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29097N.
PTM databases
PhosphoSite	P29533.
Proteomic databases
PaxDb	P29533.
PRIDE	P29533.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	22329.
KEGG	mmu:22329.
Organism-specific databases
CTD	7412.
MGI	MGI:98926. Vcam1.
Phylogenomic databases
eggNOG	NOG148090.
HOGENOM	HOG000004820.
HOVERGEN	HBG053965.
InParanoid	P29533.
KO	K06527.
OrthoDB	EOG4894M1.
Gene expression databases
CleanEx	MM_VCAM1.
Genevestigator	P29533.
GermOnline	ENSMUSG00000027962. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 7 hits.
InterPro	IPR003987. ICAM_VCAM_N. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR008424. Ig_C2-set. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR003989. VCAM-1. [Graphical view]
Pfam	PF05790. C2-set. 2 hits. PF07679. I-set. 3 hits. [Graphical view]
PRINTS	PR01472. ICAMVCAM1. PR01474. VCAM1.
SMART	SM00409. IG. 2 hits. SM00408. IGc2. 3 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 5 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	302567.
SOURCE	Search...

Entry information

Entry name

VCAM1_MOUSE

Accession

Primary (citable) accession number: P29533

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program