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P29523

- PPB_BOMMO

UniProt

P29523 - PPB_BOMMO

Protein

Membrane-bound alkaline phosphatase

Gene

Alp-m

Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 4 (18 Sep 2013)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi83 – 831MagnesiumBy similarity
    Metal bindingi83 – 831Zinc 2By similarity
    Active sitei133 – 1331Phosphoserine intermediatePROSITE-ProRule annotation
    Metal bindingi196 – 1961MagnesiumBy similarity
    Metal bindingi198 – 1981MagnesiumBy similarity
    Metal bindingi356 – 3561MagnesiumBy similarity
    Metal bindingi361 – 3611Zinc 1By similarity
    Metal bindingi365 – 3651Zinc 1By similarity
    Metal bindingi402 – 4021Zinc 2By similarity
    Metal bindingi403 – 4031Zinc 2By similarity
    Metal bindingi479 – 4791Zinc 1By similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Membrane-bound alkaline phosphatase (EC:3.1.3.1)
    Short name:
    M-ALP
    Gene namesi
    Name:Alp-m
    OrganismiBombyx mori (Silk moth)
    Taxonomic identifieri7091 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
    ProteomesiUP000005204: Unplaced

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 39391 PublicationAdd
    BLAST
    Chaini40 – 524485Membrane-bound alkaline phosphatasePRO_0000024047Add
    BLAST
    Propeptidei525 – 55026Removed in mature formSequence AnalysisPRO_0000024048Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi524 – 5241GPI-anchor amidated serineSequence Analysis

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Expressioni

    Tissue specificityi

    Midgut.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi10 – 7465Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    KOiK01077.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29523-1 [UniParc]FASTAAdd to Basket

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    MSTWWLVVVA AAAAAGLVRA EDRYHPERLA AGEASAATRS AAESEASFWV    50
    REAQEAIERR EREGAGAKQA AGHAKNVVMF LGDGMSVPTL AAARTLLGQR 100
    RGQTGEEASL HFEQFPTLGL AKTYCVNAQV PDSSCTATAY LCGVKANQGT 150
    LGVTAAVPRH DCEASTDVTK RVQSIAEWAL ADGRDVGIVT TTRITHASPA 200
    GTFAKVANRN WENDNDVKQE GHDVNRCPDI AHQLIKMAPG NKFKVIFGGG 250
    RREFLPTTQV DEEGTRGLRT DGRNLIEEWQ NDKESQKVSY KYLWNRQELL 300
    KLGSSPPDYL LGLFEGSHLQ YHLEGDESTE PTLAELTDVA IRVLSRNERG 350
    FFLFVEGGRI DHAHHDNYAH LALDETIEMD RAVKVATDAL KEDESLVVVT 400
    ADHTHVMSFN GYSPRGTDVL GTVRSLDSNR MPFMVLSYTN GPGARIQQNG 450
    VRPDVTTDAN FGALRWRTHT DVPLDSETHG GDDVTVFAWG VHHWMFSGLY 500
    EQTHVPHRMA WAACMGPGRH VCVSAATVPT AALLSLLLAA FITLRHQCFL 550
    Length:550
    Mass (Da):60,281
    Last modified:September 18, 2013 - v4
    Checksum:iD28C778B538EC96B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90454 mRNA. Translation: BAA14420.4.
    AB055428 Genomic DNA. Translation: BAB62745.3.
    PIRiS19607.
    RefSeqiNP_001037536.3. NM_001044071.3.
    UniGeneiBmo.17535.

    Genome annotation databases

    GeneIDi693074.
    KEGGibmor:693074.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90454 mRNA. Translation: BAA14420.4 .
    AB055428 Genomic DNA. Translation: BAB62745.3 .
    PIRi S19607.
    RefSeqi NP_001037536.3. NM_001044071.3.
    UniGenei Bmo.17535.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 693074.
    KEGGi bmor:693074.

    Organism-specific databases

    CTDi 693074.

    Phylogenomic databases

    KOi K01077.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of membrane-bound alkaline phosphatase cDNA of the silkworm, Bombyx mori."
      Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
      Biochim. Biophys. Acta 1129:135-138(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-46.
      Strain: Aojuku.
      Tissue: Midgut.
    2. Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
      Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 151 AND N-TERMINUS.
    3. "Tandem duplication of alkaline phosphatase genes and polymorphism in the intergenic sequence in Bombyx mori."
      Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.
      Mol. Genet. Genomics 270:114-120(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Aojuku.
      Tissue: Midgut.
    4. Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.
      Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 59; 151; 439 AND N-TERMINUS.

    Entry informationi

    Entry nameiPPB_BOMMO
    AccessioniPrimary (citable) accession number: P29523
    Secondary accession number(s): Q966T9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: September 18, 2013
    Last modified: October 1, 2014
    This is version 98 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3