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P29523

- PPB_BOMMO

UniProt

P29523 - PPB_BOMMO

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Protein

Membrane-bound alkaline phosphatase

Gene

Alp-m

Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg(2+) ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn(2+) ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi83 – 831MagnesiumBy similarity
Metal bindingi83 – 831Zinc 2By similarity
Active sitei133 – 1331Phosphoserine intermediatePROSITE-ProRule annotation
Metal bindingi196 – 1961MagnesiumBy similarity
Metal bindingi198 – 1981MagnesiumBy similarity
Metal bindingi356 – 3561MagnesiumBy similarity
Metal bindingi361 – 3611Zinc 1By similarity
Metal bindingi365 – 3651Zinc 1By similarity
Metal bindingi402 – 4021Zinc 2By similarity
Metal bindingi403 – 4031Zinc 2By similarity
Metal bindingi479 – 4791Zinc 1By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound alkaline phosphatase (EC:3.1.3.1)
Short name:
M-ALP
Gene namesi
Name:Alp-m
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
ProteomesiUP000005204: Unplaced

Subcellular locationi

Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 39391 PublicationAdd
BLAST
Chaini40 – 524485Membrane-bound alkaline phosphatasePRO_0000024047Add
BLAST
Propeptidei525 – 55026Removed in mature formSequence AnalysisPRO_0000024048Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi524 – 5241GPI-anchor amidated serineSequence Analysis

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Midgut.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi10 – 7465Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiP29523.
KOiK01077.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29523-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTWWLVVVA AAAAAGLVRA EDRYHPERLA AGEASAATRS AAESEASFWV
60 70 80 90 100
REAQEAIERR EREGAGAKQA AGHAKNVVMF LGDGMSVPTL AAARTLLGQR
110 120 130 140 150
RGQTGEEASL HFEQFPTLGL AKTYCVNAQV PDSSCTATAY LCGVKANQGT
160 170 180 190 200
LGVTAAVPRH DCEASTDVTK RVQSIAEWAL ADGRDVGIVT TTRITHASPA
210 220 230 240 250
GTFAKVANRN WENDNDVKQE GHDVNRCPDI AHQLIKMAPG NKFKVIFGGG
260 270 280 290 300
RREFLPTTQV DEEGTRGLRT DGRNLIEEWQ NDKESQKVSY KYLWNRQELL
310 320 330 340 350
KLGSSPPDYL LGLFEGSHLQ YHLEGDESTE PTLAELTDVA IRVLSRNERG
360 370 380 390 400
FFLFVEGGRI DHAHHDNYAH LALDETIEMD RAVKVATDAL KEDESLVVVT
410 420 430 440 450
ADHTHVMSFN GYSPRGTDVL GTVRSLDSNR MPFMVLSYTN GPGARIQQNG
460 470 480 490 500
VRPDVTTDAN FGALRWRTHT DVPLDSETHG GDDVTVFAWG VHHWMFSGLY
510 520 530 540 550
EQTHVPHRMA WAACMGPGRH VCVSAATVPT AALLSLLLAA FITLRHQCFL
Length:550
Mass (Da):60,281
Last modified:September 18, 2013 - v4
Checksum:iD28C778B538EC96B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90454 mRNA. Translation: BAA14420.4.
AB055428 Genomic DNA. Translation: BAB62745.3.
PIRiS19607.
RefSeqiNP_001037536.3. NM_001044071.3.
UniGeneiBmo.17535.

Genome annotation databases

GeneIDi693074.
KEGGibmor:693074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90454 mRNA. Translation: BAA14420.4 .
AB055428 Genomic DNA. Translation: BAB62745.3 .
PIRi S19607.
RefSeqi NP_001037536.3. NM_001044071.3.
UniGenei Bmo.17535.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 693074.
KEGGi bmor:693074.

Organism-specific databases

CTDi 693074.

Phylogenomic databases

InParanoidi P29523.
KOi K01077.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence analysis of membrane-bound alkaline phosphatase cDNA of the silkworm, Bombyx mori."
    Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
    Biochim. Biophys. Acta 1129:135-138(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-46.
    Strain: Aojuku.
    Tissue: Midgut.
  2. Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
    Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 151 AND N-TERMINUS.
  3. "Tandem duplication of alkaline phosphatase genes and polymorphism in the intergenic sequence in Bombyx mori."
    Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.
    Mol. Genet. Genomics 270:114-120(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Aojuku.
    Tissue: Midgut.
  4. Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.
    Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 59; 151; 439 AND N-TERMINUS.

Entry informationi

Entry nameiPPB_BOMMO
AccessioniPrimary (citable) accession number: P29523
Secondary accession number(s): Q966T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: September 18, 2013
Last modified: November 26, 2014
This is version 100 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3