Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P29523 (PPB_BOMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Membrane-bound alkaline phosphatase

Short name=M-ALP
EC=3.1.3.1
Gene names
Name:Alp-m
OrganismBombyx mori (Silk moth) [Reference proteome]
Taxonomic identifier7091 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx

Protein attributes

Sequence length550 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor By similarity.

Tissue specificity

Midgut.

Sequence similarities

Belongs to the alkaline phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 Ref.1
Chain40 – 524485Membrane-bound alkaline phosphatase
PRO_0000024047
Propeptide525 – 55026Removed in mature form Potential
PRO_0000024048

Regions

Compositional bias10 – 7465Ala-rich

Sites

Active site1331Phosphoserine intermediate By similarity
Metal binding831Magnesium By similarity
Metal binding831Zinc 2 By similarity
Metal binding1961Magnesium By similarity
Metal binding1981Magnesium By similarity
Metal binding3561Magnesium By similarity
Metal binding3611Zinc 1 By similarity
Metal binding3651Zinc 1 By similarity
Metal binding4021Zinc 2 By similarity
Metal binding4031Zinc 2 By similarity
Metal binding4791Zinc 1 By similarity

Amino acid modifications

Lipidation5241GPI-anchor amidated serine Potential

Sequences

Sequence LengthMass (Da)Tools
P29523 [UniParc].

Last modified September 18, 2013. Version 4.
Checksum: D28C778B538EC96B

FASTA55060,281
        10         20         30         40         50         60 
MSTWWLVVVA AAAAAGLVRA EDRYHPERLA AGEASAATRS AAESEASFWV REAQEAIERR 

        70         80         90        100        110        120 
EREGAGAKQA AGHAKNVVMF LGDGMSVPTL AAARTLLGQR RGQTGEEASL HFEQFPTLGL 

       130        140        150        160        170        180 
AKTYCVNAQV PDSSCTATAY LCGVKANQGT LGVTAAVPRH DCEASTDVTK RVQSIAEWAL 

       190        200        210        220        230        240 
ADGRDVGIVT TTRITHASPA GTFAKVANRN WENDNDVKQE GHDVNRCPDI AHQLIKMAPG 

       250        260        270        280        290        300 
NKFKVIFGGG RREFLPTTQV DEEGTRGLRT DGRNLIEEWQ NDKESQKVSY KYLWNRQELL 

       310        320        330        340        350        360 
KLGSSPPDYL LGLFEGSHLQ YHLEGDESTE PTLAELTDVA IRVLSRNERG FFLFVEGGRI 

       370        380        390        400        410        420 
DHAHHDNYAH LALDETIEMD RAVKVATDAL KEDESLVVVT ADHTHVMSFN GYSPRGTDVL 

       430        440        450        460        470        480 
GTVRSLDSNR MPFMVLSYTN GPGARIQQNG VRPDVTTDAN FGALRWRTHT DVPLDSETHG 

       490        500        510        520        530        540 
GDDVTVFAWG VHHWMFSGLY EQTHVPHRMA WAACMGPGRH VCVSAATVPT AALLSLLLAA 

       550 
FITLRHQCFL 

« Hide

References

[1]"Cloning and sequence analysis of membrane-bound alkaline phosphatase cDNA of the silkworm, Bombyx mori."
Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
Biochim. Biophys. Acta 1129:135-138(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-46.
Strain: Aojuku.
Tissue: Midgut.
[2]Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 151 AND N-TERMINUS.
[3]"Tandem duplication of alkaline phosphatase genes and polymorphism in the intergenic sequence in Bombyx mori."
Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.
Mol. Genet. Genomics 270:114-120(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Aojuku.
Tissue: Midgut.
[4]Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.
Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 59; 151; 439 AND N-TERMINUS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D90454 mRNA. Translation: BAA14420.4.
AB055428 Genomic DNA. Translation: BAB62745.3.
PIRS19607.
RefSeqNP_001037536.3. NM_001044071.3.
UniGeneBmo.17535.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID693074.
KEGGbmor:693074.

Organism-specific databases

CTD693074.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. SSF53649. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPB_BOMMO
AccessionPrimary (citable) accession number: P29523
Secondary accession number(s): Q966T9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: September 18, 2013
Last modified: July 9, 2014
This is version 96 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families