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Protein

Membrane-bound alkaline phosphatase

Gene

Alp-m

Organism
Bombyx mori (Silk moth)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83MagnesiumBy similarity1
Metal bindingi83Zinc 2By similarity1
Active sitei133Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi196MagnesiumBy similarity1
Metal bindingi198MagnesiumBy similarity1
Metal bindingi356MagnesiumBy similarity1
Metal bindingi361Zinc 1By similarity1
Metal bindingi365Zinc 1By similarity1
Metal bindingi402Zinc 2By similarity1
Metal bindingi403Zinc 2By similarity1
Metal bindingi479Zinc 1By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound alkaline phosphatase (EC:3.1.3.1)
Short name:
M-ALP
Gene namesi
Name:Alp-m
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
Proteomesi
  • UP000005204 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 391 PublicationAdd BLAST39
ChainiPRO_000002404740 – 524Membrane-bound alkaline phosphataseAdd BLAST485
PropeptideiPRO_0000024048525 – 550Removed in mature formSequence analysisAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi524GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Midgut.

Interactioni

Protein-protein interaction databases

STRINGi7091.BGIBMGA008818-TA.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 74Ala-richAdd BLAST65

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
InParanoidiP29523.
KOiK01077.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29523-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTWWLVVVA AAAAAGLVRA EDRYHPERLA AGEASAATRS AAESEASFWV
60 70 80 90 100
REAQEAIERR EREGAGAKQA AGHAKNVVMF LGDGMSVPTL AAARTLLGQR
110 120 130 140 150
RGQTGEEASL HFEQFPTLGL AKTYCVNAQV PDSSCTATAY LCGVKANQGT
160 170 180 190 200
LGVTAAVPRH DCEASTDVTK RVQSIAEWAL ADGRDVGIVT TTRITHASPA
210 220 230 240 250
GTFAKVANRN WENDNDVKQE GHDVNRCPDI AHQLIKMAPG NKFKVIFGGG
260 270 280 290 300
RREFLPTTQV DEEGTRGLRT DGRNLIEEWQ NDKESQKVSY KYLWNRQELL
310 320 330 340 350
KLGSSPPDYL LGLFEGSHLQ YHLEGDESTE PTLAELTDVA IRVLSRNERG
360 370 380 390 400
FFLFVEGGRI DHAHHDNYAH LALDETIEMD RAVKVATDAL KEDESLVVVT
410 420 430 440 450
ADHTHVMSFN GYSPRGTDVL GTVRSLDSNR MPFMVLSYTN GPGARIQQNG
460 470 480 490 500
VRPDVTTDAN FGALRWRTHT DVPLDSETHG GDDVTVFAWG VHHWMFSGLY
510 520 530 540 550
EQTHVPHRMA WAACMGPGRH VCVSAATVPT AALLSLLLAA FITLRHQCFL
Length:550
Mass (Da):60,281
Last modified:September 18, 2013 - v4
Checksum:iD28C778B538EC96B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90454 mRNA. Translation: BAA14420.4.
AB055428 Genomic DNA. Translation: BAB62745.3.
PIRiS19607.
RefSeqiNP_001037536.3. NM_001044071.3.
UniGeneiBmo.17535.

Genome annotation databases

GeneIDi693074.
KEGGibmor:693074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90454 mRNA. Translation: BAA14420.4.
AB055428 Genomic DNA. Translation: BAB62745.3.
PIRiS19607.
RefSeqiNP_001037536.3. NM_001044071.3.
UniGeneiBmo.17535.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7091.BGIBMGA008818-TA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi693074.
KEGGibmor:693074.

Organism-specific databases

CTDi693074.

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
InParanoidiP29523.
KOiK01077.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPB_BOMMO
AccessioniPrimary (citable) accession number: P29523
Secondary accession number(s): Q966T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: September 18, 2013
Last modified: November 30, 2016
This is version 105 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.