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Reviewed, UniProtKB/Swiss-Prot P29523 (PPB_BOMMO)

Last modified June 16, 2009. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Membrane-bound alkaline phosphatase
      Short name=M-ALP
    EC=3.1.3.1
Gene names
Name: Alp-m
OrganismBombyx mori (Silk moth)
Taxonomic identifier7091 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Lepidoptera › Glossata › Ditrysia › Bombycoidea › Bombycidae › Bombycinae › Bombyx

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Protein attributes

Sequence length550 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor By similarity.

Tissue specificity

Midgut.

Sequence similarities

Belongs to the alkaline phosphatase family.

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Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMGPI-anchor
Glycoprotein
Lipoprotein
Phosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentanchored to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalkaline phosphatase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 Ref.1
Chain40 – 516477Membrane-bound alkaline phosphatase
PRO_0000024047
Propeptide517 – 55034Removed in mature form Potential
PRO_0000024048

Regions

Compositional bias10 – 7465Ala-rich

Sites

Active site1331Phosphoserine intermediate By similarity
Metal binding831Magnesium By similarity
Metal binding831Zinc 2 By similarity
Metal binding1961Magnesium By similarity
Metal binding1981Magnesium By similarity
Metal binding3561Magnesium By similarity
Metal binding3611Zinc 1 By similarity
Metal binding3651Zinc 1 By similarity
Metal binding4021Zinc 2 By similarity
Metal binding4031Zinc 2 By similarity
Metal binding4791Zinc 1 By similarity

Amino acid modifications

Lipidation5161GPI-anchor amidated glycine Potential

Experimental info

Sequence conflict591T → R Ref.1
Sequence conflict591T → R in BAA14420. Ref.2
Sequence conflict4391A → T Ref.1
Sequence conflict4391A → T in BAA14420. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P29523-1 [UniParc].

Last modified November 28, 2006. Version 3.
Checksum: C3F4C95A7F69CD57

FASTA55060,179
        10         20         30         40         50         60 
MSTWWLVVVA AAAAAGLVRA EDRYHPERLA AGEASAATRS AAESEASFWV REAQEAIETR 

        70         80         90        100        110        120 
EREGAGAKQA AGHAKNVVMF LGDGMSVPTL AAARTLLGQR RGQTGEEASL HFEQFPTLGL 

       130        140        150        160        170        180 
AKTYCVNAQV PDSSCTATAY LCGVKANQGT PGVTAAVPRH DCEASTDVTK RVQSIAEWAL 

       190        200        210        220        230        240 
ADGRDVGIVT TTRITHASPA GTFAKVANRN WENDNDVKQE GHDVNRCPDI AHQLIKMAPG 

       250        260        270        280        290        300 
NKFKVIFGGG RREFLPTTQV DEEGTRGLRT DGRNLIEEWQ NDKESQKVSY KYLWNRQELL 

       310        320        330        340        350        360 
KLGSSPPDYL LGLFEGSHLQ YHLEGDESTE PTLAELTDVA IRVLSRNERG FFLFVEGGRI 

       370        380        390        400        410        420 
DHAHHDNYAH LALDETIEMD RAVKVATDAL KEDESLVVVT ADHTHVMSFN GYSPRGTDVL 

       430        440        450        460        470        480 
GTVRSLDSNR MPFMVLSYAN GPGARIQQNG VRPDVTTDAN FGALRWRTHT DVPLDSETHG 

       490        500        510        520        530        540 
GDDVTVFAWG VHHWMFSGLY EQTHVPHRMA WAACMGPGRH VCVSAATVPT AALLSLLLAA 

       550 
FITLRHQCFL

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References

[1]"Cloning and sequence analysis of membrane-bound alkaline phosphatase cDNA of the silkworm, Bombyx mori."
Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
Biochim. Biophys. Acta 1129:135-138(1991) [PubMed: 1756175] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-46.
Strain: Aojuku.
Tissue: Midgut.
[2]Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]"Tandem duplication of alkaline phosphatase genes and polymorphism in the intergenic sequence in Bombyx mori."
Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.
Mol. Genet. Genomics 270:114-120(2003) [PubMed: 14508679] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Aojuku.
Tissue: Midgut.
[4]Itoh M., Kanamori Y., Nishida S.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO N-TERMINUS.

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Cross-references

Sequence databases

D90454 mRNA. Translation: BAA14420.3.
AB055428 Genomic DNA. Translation: BAB62745.2.
PIRS19607.
RefSeqNP_001037536.2.
UniGeneBmo.716

3D structure databases

HSSPHSSP built from PDB template 1EW2 based on UniProtKB P05187.
ModBaseSearch...

Genome annotation databases

GeneID693074.

Enzyme and pathway databases

BRENDA3.1.3.1. 252.

Family and domain databases

InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
[Graphical view]
Gene3DG3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePPB_BOMMO
AccessionPrimary (citable) accession number: P29523
Secondary accession number(s): Q966T9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 28, 2006
Last modified: June 16, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents