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P29523

- PPB_BOMMO

UniProt

P29523 - PPB_BOMMO

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Protein
Membrane-bound alkaline phosphatase
Gene
Alp-m
Organism
Bombyx mori (Silk moth)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 magnesium ion By similarity.
Binds 2 zinc ions By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi83 – 831Magnesium By similarity
Metal bindingi83 – 831Zinc 2 By similarity
Active sitei133 – 1331Phosphoserine intermediate By similarity
Metal bindingi196 – 1961Magnesium By similarity
Metal bindingi198 – 1981Magnesium By similarity
Metal bindingi356 – 3561Magnesium By similarity
Metal bindingi361 – 3611Zinc 1 By similarity
Metal bindingi365 – 3651Zinc 1 By similarity
Metal bindingi402 – 4021Zinc 2 By similarity
Metal bindingi403 – 4031Zinc 2 By similarity
Metal bindingi479 – 4791Zinc 1 By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound alkaline phosphatase (EC:3.1.3.1)
Short name:
M-ALP
Gene namesi
Name:Alp-m
OrganismiBombyx mori (Silk moth)
Taxonomic identifieri7091 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx
ProteomesiUP000005204: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 39391 Publication
Add
BLAST
Chaini40 – 524485Membrane-bound alkaline phosphatase
PRO_0000024047Add
BLAST
Propeptidei525 – 55026Removed in mature form Reviewed prediction
PRO_0000024048Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi524 – 5241GPI-anchor amidated serine Reviewed prediction

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Midgut.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi10 – 7465Ala-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK01077.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29523-1 [UniParc]FASTAAdd to Basket

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MSTWWLVVVA AAAAAGLVRA EDRYHPERLA AGEASAATRS AAESEASFWV    50
REAQEAIERR EREGAGAKQA AGHAKNVVMF LGDGMSVPTL AAARTLLGQR 100
RGQTGEEASL HFEQFPTLGL AKTYCVNAQV PDSSCTATAY LCGVKANQGT 150
LGVTAAVPRH DCEASTDVTK RVQSIAEWAL ADGRDVGIVT TTRITHASPA 200
GTFAKVANRN WENDNDVKQE GHDVNRCPDI AHQLIKMAPG NKFKVIFGGG 250
RREFLPTTQV DEEGTRGLRT DGRNLIEEWQ NDKESQKVSY KYLWNRQELL 300
KLGSSPPDYL LGLFEGSHLQ YHLEGDESTE PTLAELTDVA IRVLSRNERG 350
FFLFVEGGRI DHAHHDNYAH LALDETIEMD RAVKVATDAL KEDESLVVVT 400
ADHTHVMSFN GYSPRGTDVL GTVRSLDSNR MPFMVLSYTN GPGARIQQNG 450
VRPDVTTDAN FGALRWRTHT DVPLDSETHG GDDVTVFAWG VHHWMFSGLY 500
EQTHVPHRMA WAACMGPGRH VCVSAATVPT AALLSLLLAA FITLRHQCFL 550
Length:550
Mass (Da):60,281
Last modified:September 18, 2013 - v4
Checksum:iD28C778B538EC96B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90454 mRNA. Translation: BAA14420.4.
AB055428 Genomic DNA. Translation: BAB62745.3.
PIRiS19607.
RefSeqiNP_001037536.3. NM_001044071.3.
UniGeneiBmo.17535.

Genome annotation databases

GeneIDi693074.
KEGGibmor:693074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D90454 mRNA. Translation: BAA14420.4 .
AB055428 Genomic DNA. Translation: BAB62745.3 .
PIRi S19607.
RefSeqi NP_001037536.3. NM_001044071.3.
UniGenei Bmo.17535.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 693074.
KEGGi bmor:693074.

Organism-specific databases

CTDi 693074.

Phylogenomic databases

KOi K01077.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence analysis of membrane-bound alkaline phosphatase cDNA of the silkworm, Bombyx mori."
    Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
    Biochim. Biophys. Acta 1129:135-138(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 40-46.
    Strain: Aojuku.
    Tissue: Midgut.
  2. Itoh M., Takeda S., Yamamoto H., Izumi S., Tomino S., Eguchi M.
    Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 151 AND N-TERMINUS.
  3. "Tandem duplication of alkaline phosphatase genes and polymorphism in the intergenic sequence in Bombyx mori."
    Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.
    Mol. Genet. Genomics 270:114-120(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Aojuku.
    Tissue: Midgut.
  4. Itoh M., Inoue T., Kanamori Y., Nishida S., Yamaguchi M.
    Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 59; 151; 439 AND N-TERMINUS.

Entry informationi

Entry nameiPPB_BOMMO
AccessioniPrimary (citable) accession number: P29523
Secondary accession number(s): Q966T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: September 18, 2013
Last modified: September 3, 2014
This is version 97 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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