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Protein

Tubulin beta-8 chain

Gene

TUBB8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 146GTPSequence analysis7

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: TAIR

GO - Biological processi

  • microtubule-based process Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin beta-8 chain
Alternative name(s):
Beta-8-tubulin
Gene namesi
Name:TUBB8
Synonyms:TUB8
Ordered Locus Names:At5g23860
ORF Names:MRO11.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G23860.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: TAIR
  • membrane Source: TAIR
  • microtubule Source: UniProtKB-KW
  • tubulin complex Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000483271 – 449Tubulin beta-8 chainAdd BLAST449

Proteomic databases

PaxDbiP29516.
PRIDEiP29516.

Expressioni

Gene expression databases

GenevisibleiP29516. AT.

Interactioni

Subunit structurei

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Protein-protein interaction databases

BioGridi17726. 7 interactors.
IntActiP29516. 1 interactor.
STRINGi3702.AT5G23860.1.

Structurei

3D structure databases

ProteinModelPortaliP29516.
SMRiP29516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tubulin family.Curated

Phylogenomic databases

eggNOGiKOG1375. Eukaryota.
COG5023. LUCA.
HOGENOMiHOG000165710.
InParanoidiP29516.
KOiK07375.
OMAiQRELLWH.
OrthoDBiEOG093609OI.
PhylomeDBiP29516.

Family and domain databases

Gene3Di1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProiIPR013838. Beta-tubulin_BS.
IPR002453. Beta_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERiPTHR11588. PTHR11588. 1 hit.
PfamiPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSiPR01163. BETATUBULIN.
PR01161. TUBULIN.
SMARTiSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMiSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEiPS00227. TUBULIN. 1 hit.
PS00228. TUBULIN_B_AUTOREG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREILHIQGG QCGNQIGAKF WEVVCAEHGI DSTGRYQGEN DLQLERVNVY
60 70 80 90 100
YNEASCGRFV PRAVLMDLEP GTMDSVRSGP YGQIFRPDNF VFGQSGAGNN
110 120 130 140 150
WAKGHYTEGA ELIDSVLDVV RKEAENCDCL QGFQVCHSLG GGTGSGMGTL
160 170 180 190 200
LISKIREEYP DRMMLTFSVF PSPKVSDTVV EPYNATLSVH QLVENADECM
210 220 230 240 250
VLDNEALYDI CFRTLKLTTP SFGDLNHLIS ATMSGVTCCL RFPGQLNSDL
260 270 280 290 300
RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YRALTVPELT QQMWDAKNMM
310 320 330 340 350
CAADPRHGRY LTASAMFRGK MSTKEVDEQM INVQNKNSSY FVEWIPNNVK
360 370 380 390 400
STVCDIPPTG LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG
410 420 430 440
EGMDEMEFTE AESNMNDLVS EYQQYQDATA DEEEGYEYEE DEVEVQEEQ
Length:449
Mass (Da):50,607
Last modified:January 10, 2003 - v2
Checksum:iC66EBE7C8FB18F05
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40N → K in AAM10035 (PubMed:14593172).Curated1
Sequence conflicti40N → K in AAK96884 (PubMed:14593172).Curated1
Sequence conflicti124A → R in AAA32886 (PubMed:1498609).Curated1
Sequence conflicti164 – 165ML → IV in AAA32886 (PubMed:1498609).Curated2
Sequence conflicti170F → I in AAA32886 (PubMed:1498609).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84705 Genomic DNA. Translation: AAA32886.1.
AB005244 Genomic DNA. Translation: BAB10059.1.
CP002688 Genomic DNA. Translation: AED93223.1.
CP002688 Genomic DNA. Translation: AED93224.1.
AY054693 mRNA. Translation: AAK96884.1.
AY081473 mRNA. Translation: AAM10035.1.
PIRiJQ1592.
RefSeqiNP_001190373.1. NM_001203444.1.
NP_568437.1. NM_122291.4.
UniGeneiAt.330.
At.67084.

Genome annotation databases

EnsemblPlantsiAT5G23860.1; AT5G23860.1; AT5G23860.
AT5G23860.2; AT5G23860.2; AT5G23860.
GeneIDi832451.
GrameneiAT5G23860.1; AT5G23860.1; AT5G23860.
AT5G23860.2; AT5G23860.2; AT5G23860.
KEGGiath:AT5G23860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84705 Genomic DNA. Translation: AAA32886.1.
AB005244 Genomic DNA. Translation: BAB10059.1.
CP002688 Genomic DNA. Translation: AED93223.1.
CP002688 Genomic DNA. Translation: AED93224.1.
AY054693 mRNA. Translation: AAK96884.1.
AY081473 mRNA. Translation: AAM10035.1.
PIRiJQ1592.
RefSeqiNP_001190373.1. NM_001203444.1.
NP_568437.1. NM_122291.4.
UniGeneiAt.330.
At.67084.

3D structure databases

ProteinModelPortaliP29516.
SMRiP29516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17726. 7 interactors.
IntActiP29516. 1 interactor.
STRINGi3702.AT5G23860.1.

Proteomic databases

PaxDbiP29516.
PRIDEiP29516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G23860.1; AT5G23860.1; AT5G23860.
AT5G23860.2; AT5G23860.2; AT5G23860.
GeneIDi832451.
GrameneiAT5G23860.1; AT5G23860.1; AT5G23860.
AT5G23860.2; AT5G23860.2; AT5G23860.
KEGGiath:AT5G23860.

Organism-specific databases

TAIRiAT5G23860.

Phylogenomic databases

eggNOGiKOG1375. Eukaryota.
COG5023. LUCA.
HOGENOMiHOG000165710.
InParanoidiP29516.
KOiK07375.
OMAiQRELLWH.
OrthoDBiEOG093609OI.
PhylomeDBiP29516.

Miscellaneous databases

PROiP29516.

Gene expression databases

GenevisibleiP29516. AT.

Family and domain databases

Gene3Di1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProiIPR013838. Beta-tubulin_BS.
IPR002453. Beta_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERiPTHR11588. PTHR11588. 1 hit.
PfamiPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSiPR01163. BETATUBULIN.
PR01161. TUBULIN.
SMARTiSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMiSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEiPS00227. TUBULIN. 1 hit.
PS00228. TUBULIN_B_AUTOREG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTBB8_ARATH
AccessioniPrimary (citable) accession number: P29516
Secondary accession number(s): Q93Y15, Q9FF94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 10, 2003
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are nine genes coding for beta-tubulin.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.