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P29516 (TBB8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tubulin beta-8 chain
Alternative name(s):
Beta-8-tubulin
Gene names
Name:TUBB8
Synonyms:TUB8
Ordered Locus Names:At5g23860
ORF Names:MRO11.10
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Subunit structure

Dimer of alpha and beta chains.

Subcellular location

Cytoplasm › cytoskeleton.

Miscellaneous

There are nine genes coding for beta-tubulin.

Sequence similarities

Belongs to the tubulin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Tubulin beta-8 chain
PRO_0000048327

Regions

Nucleotide binding140 – 1467GTP Potential

Experimental info

Sequence conflict401N → K in AAM10035. Ref.4
Sequence conflict401N → K in AAK96884. Ref.4
Sequence conflict1241A → R in AAA32886. Ref.1
Sequence conflict164 – 1652ML → IV in AAA32886. Ref.1
Sequence conflict1701F → I in AAA32886. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P29516 [UniParc].

Last modified January 10, 2003. Version 2.
Checksum: C66EBE7C8FB18F05

FASTA44950,607
        10         20         30         40         50         60 
MREILHIQGG QCGNQIGAKF WEVVCAEHGI DSTGRYQGEN DLQLERVNVY YNEASCGRFV 

        70         80         90        100        110        120 
PRAVLMDLEP GTMDSVRSGP YGQIFRPDNF VFGQSGAGNN WAKGHYTEGA ELIDSVLDVV 

       130        140        150        160        170        180 
RKEAENCDCL QGFQVCHSLG GGTGSGMGTL LISKIREEYP DRMMLTFSVF PSPKVSDTVV 

       190        200        210        220        230        240 
EPYNATLSVH QLVENADECM VLDNEALYDI CFRTLKLTTP SFGDLNHLIS ATMSGVTCCL 

       250        260        270        280        290        300 
RFPGQLNSDL RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YRALTVPELT QQMWDAKNMM 

       310        320        330        340        350        360 
CAADPRHGRY LTASAMFRGK MSTKEVDEQM INVQNKNSSY FVEWIPNNVK STVCDIPPTG 

       370        380        390        400        410        420 
LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS 

       430        440 
EYQQYQDATA DEEEGYEYEE DEVEVQEEQ

« Hide

References

« Hide 'large scale' references
[1]"The small genome of Arabidopsis contains at least nine expressed beta-tubulin genes."
Snustad D.P., Haas N.A., Kopczak S.D., Silflow C.D.
Plant Cell 4:549-556(1992) [PubMed: 1498609] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed: 9330910] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M84705 Genomic DNA. Translation: AAA32886.1.
AB005244 Genomic DNA. Translation: BAB10059.1.
CP002688 Genomic DNA. Translation: AED93223.1.
CP002688 Genomic DNA. Translation: AED93224.1.
AY054693 mRNA. Translation: AAK96884.1.
AY081473 mRNA. Translation: AAM10035.1.
IPIIPI00539093.
PIRJQ1592.
RefSeqNP_001190373.1. NM_001203444.1.
NP_568437.1. NM_122291.3.
UniGeneAt.330.
At.67084.

3D structure databases

ProteinModelPortalP29516.
SMRP29516. Positions 2-427.
ModBaseSearch...

Protein-protein interaction databases

IntActP29516. 1 interaction.
STRINGP29516.

Proteomic databases

PRIDEP29516.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G23860.1; AT5G23860.1; AT5G23860.
AT5G23860.2; AT5G23860.2; AT5G23860.
GeneID832451.
GenomeReviewsGene locus AT5G23860 in contig BA000015_GR.
KEGGath:AT5G23860.
NMPDRfig|3702.1.peg.24566.

Organism-specific databases

TAIRAt5g23860.

Phylogenomic databases

eggNOGKOG1375.
GeneTreeEPGT00050000007628.
HOGENOMHBG750007.
InParanoidP29516.
OMARYQGEND.
PhylomeDBP29516.
ProtClustDBPLN00220.

Gene expression databases

GenevestigatorP29516.
GermOnlineAT5G23860. Arabidopsis thaliana.

Family and domain databases

InterProIPR013838. Beta-tubulin_BS.
IPR002453. Beta_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
Gene3DG3DSA:3.30.1330.20. Tubulin/FtsZ_2-layer-sand-dom. 1 hit.
G3DSA:1.10.287.600. Tubulin_C. 1 hit.
G3DSA:3.40.50.1440. Tubulin_FtsZ. 1 hit.
KOK07375.
PfamPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSPR01163. BETATUBULIN.
PR01161. TUBULIN.
SMARTSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMSSF55307. Tub_FtsZ_C. 1 hit.
SSF52490. Tubulin_FtsZ. 1 hit.
PROSITEPS00227. TUBULIN. 1 hit.
PS00228. TUBULIN_B_AUTOREG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTBB8_ARATH
AccessionPrimary (citable) accession number: P29516
Secondary accession number(s): Q93Y15, Q9FF94
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 10, 2003
Last modified: November 16, 2011
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents