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Protein

Tubulin beta-5 chain

Gene

TUBB5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi141 – 1477GTPSequence analysis

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: TAIR

GO - Biological processi

  • microtubule-based process Source: TAIR
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin beta-5 chain
Alternative name(s):
Beta-5-tubulin
Gene namesi
Name:TUBB5
Synonyms:TUB5
Ordered Locus Names:At1g20010
ORF Names:T20H2.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G20010.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • membrane Source: TAIR
  • microtubule Source: UniProtKB-KW
  • plasma membrane Source: TAIR
  • tubulin complex Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Tubulin beta-5 chainPRO_0000048324Add
BLAST

Proteomic databases

PaxDbiP29513.
PRIDEiP29513.

Expressioni

Gene expression databases

GenevisibleiP29513. AT.

Interactioni

Subunit structurei

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Protein-protein interaction databases

BioGridi23829. 6 interactions.
IntActiP29513. 2 interactions.
STRINGi3702.AT1G20010.1.

Structurei

3D structure databases

ProteinModelPortaliP29513.
SMRiP29513. Positions 1-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tubulin family.Curated

Phylogenomic databases

eggNOGiKOG1375. Eukaryota.
COG5023. LUCA.
HOGENOMiHOG000165710.
InParanoidiP29513.
KOiK07375.
OMAiYSGDTAD.
PhylomeDBiP29513.

Family and domain databases

Gene3Di1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProiIPR013838. Beta-tubulin_BS.
IPR002453. Beta_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERiPTHR11588. PTHR11588. 1 hit.
PfamiPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSiPR01163. BETATUBULIN.
PR01161. TUBULIN.
SMARTiSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMiSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEiPS00227. TUBULIN. 1 hit.
PS00228. TUBULIN_B_AUTOREG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29513-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREILHIQGG QCGNQIGSKF WEVICDEHGI DSTGRYSGDT ADLQLERINV
60 70 80 90 100
YYNEASGGRY VPRAVLMDLE PGTMDSIRSG PFGQIFRPDN FVFGQSGAGN
110 120 130 140 150
NWAKGHYTEG AELIDAVLDV VRKEAENCDC LQGFQVCHSL GGGTGSGMGT
160 170 180 190 200
LLISKIREEY PDRMMLTFSV FPSPKVSDTV VEPYNATLSV HQLVENADEC
210 220 230 240 250
MVLDNEALYD ICFRTLKLST PSFGDLNHLI SATMSGVTCS LRFPGQLNSD
260 270 280 290 300
LRKLAVNLIP FPRLHFFMVG FAPLTSRGSQ QYISLTVPEL TQQMWDSKNM
310 320 330 340 350
MCAADPRHGR YLTASAIFRG QMSTKEVDEQ ILNIQNKNSS YFVEWIPNNV
360 370 380 390 400
KSSVCDIPPK GLKMAATFVG NSTSIQEMFR RVSEQFTAMF RRKAFLHWYT
410 420 430 440
GEGMDEMEFT EAESNMNDLV AEYQQYQDAT ADEEGEYDVE EEEEGDYET
Length:449
Mass (Da):50,342
Last modified:April 1, 1993 - v1
Checksum:iE53CDF6D764417DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84702 Genomic DNA. Translation: AAA32883.1.
AC022472 Genomic DNA. Translation: AAF79912.1.
CP002684 Genomic DNA. Translation: AEE29922.1.
AF361585 mRNA. Translation: AAK32753.1.
AY093989 mRNA. Translation: AAM16250.1.
PIRiJQ1589.
RefSeqiNP_564101.1. NM_101856.2.
UniGeneiAt.25087.

Genome annotation databases

EnsemblPlantsiAT1G20010.1; AT1G20010.1; AT1G20010.
GeneIDi838590.
GrameneiAT1G20010.1; AT1G20010.1; AT1G20010.
KEGGiath:AT1G20010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84702 Genomic DNA. Translation: AAA32883.1.
AC022472 Genomic DNA. Translation: AAF79912.1.
CP002684 Genomic DNA. Translation: AEE29922.1.
AF361585 mRNA. Translation: AAK32753.1.
AY093989 mRNA. Translation: AAM16250.1.
PIRiJQ1589.
RefSeqiNP_564101.1. NM_101856.2.
UniGeneiAt.25087.

3D structure databases

ProteinModelPortaliP29513.
SMRiP29513. Positions 1-432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23829. 6 interactions.
IntActiP29513. 2 interactions.
STRINGi3702.AT1G20010.1.

Proteomic databases

PaxDbiP29513.
PRIDEiP29513.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G20010.1; AT1G20010.1; AT1G20010.
GeneIDi838590.
GrameneiAT1G20010.1; AT1G20010.1; AT1G20010.
KEGGiath:AT1G20010.

Organism-specific databases

TAIRiAT1G20010.

Phylogenomic databases

eggNOGiKOG1375. Eukaryota.
COG5023. LUCA.
HOGENOMiHOG000165710.
InParanoidiP29513.
KOiK07375.
OMAiYSGDTAD.
PhylomeDBiP29513.

Miscellaneous databases

PROiP29513.

Gene expression databases

GenevisibleiP29513. AT.

Family and domain databases

Gene3Di1.10.287.600. 1 hit.
3.30.1330.20. 1 hit.
3.40.50.1440. 1 hit.
InterProiIPR013838. Beta-tubulin_BS.
IPR002453. Beta_tubulin.
IPR008280. Tub_FtsZ_C.
IPR000217. Tubulin.
IPR018316. Tubulin/FtsZ_2-layer-sand-dom.
IPR023123. Tubulin_C.
IPR017975. Tubulin_CS.
IPR003008. Tubulin_FtsZ_GTPase.
[Graphical view]
PANTHERiPTHR11588. PTHR11588. 1 hit.
PfamiPF00091. Tubulin. 1 hit.
PF03953. Tubulin_C. 1 hit.
[Graphical view]
PRINTSiPR01163. BETATUBULIN.
PR01161. TUBULIN.
SMARTiSM00864. Tubulin. 1 hit.
SM00865. Tubulin_C. 1 hit.
[Graphical view]
SUPFAMiSSF52490. SSF52490. 1 hit.
SSF55307. SSF55307. 1 hit.
PROSITEiPS00227. TUBULIN. 1 hit.
PS00228. TUBULIN_B_AUTOREG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The small genome of Arabidopsis contains at least nine expressed beta-tubulin genes."
    Snustad D.P., Haas N.A., Kopczak S.D., Silflow C.D.
    Plant Cell 4:549-556(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiTBB5_ARATH
AccessioniPrimary (citable) accession number: P29513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: February 17, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are nine genes coding for beta-tubulin.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.