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Protein

Serpin B3

Gene

SERPINB3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH2-terminal kinase (JNK1).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei354 – 355Reactive bond2

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: UniProtKB
  • protease binding Source: UniProtKB
  • serine-type endopeptidase inhibitor activity Source: GO_Central
  • virus receptor activity Source: UniProtKB

GO - Biological processi

  • autocrine signaling Source: UniProtKB
  • negative regulation of catalytic activity Source: UniProtKB
  • negative regulation of endopeptidase activity Source: UniProtKB
  • negative regulation of JUN kinase activity Source: UniProtKB
  • negative regulation of peptidase activity Source: UniProtKB
  • negative regulation of proteolysis Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • paracrine signaling Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of endopeptidase activity Source: UniProtKB
  • positive regulation of epithelial to mesenchymal transition Source: UniProtKB

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiI04.008

Names & Taxonomyi

Protein namesi
Recommended name:
Serpin B3
Alternative name(s):
Protein T4-A
Squamous cell carcinoma antigen 1
Short name:
SCCA-1
Gene namesi
Name:SERPINB3
Synonyms:SCCA, SCCA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000057149.14
HGNCiHGNC:10569 SERPINB3
MIMi600517 gene
neXtProtiNX_P29508

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi341A → R: Loss of inhibitory activity. 1 Publication1
Mutagenesisi352F → A: Loss of inhibitory activity. 2 Publications1
Mutagenesisi352F → G: Loss of inhibitory activity to papain but does not decrease the suppression activity to MAPK8. 2 Publications1
Mutagenesisi354 – 355SS → PP: Loss of inhibitory activity. 1 Publication2

Organism-specific databases

DisGeNETi6317
OpenTargetsiENSG00000057149
PharmGKBiPA35538

Chemistry databases

DrugBankiDB03929 D-Serine
DB04522 Phosphonoserine

Polymorphism and mutation databases

BioMutaiSERPINB3
DMDMi20141712

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000941031 – 390Serpin B3Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP29508
MaxQBiP29508
PaxDbiP29508
PeptideAtlasiP29508
PRIDEiP29508
TopDownProteomicsiP29508-1 [P29508-1]
P29508-2 [P29508-2]

2D gel databases

SWISS-2DPAGEiP29508
UCD-2DPAGEiP29508

PTM databases

iPTMnetiP29508
PhosphoSitePlusiP29508

Expressioni

Tissue specificityi

Squamous cells. Expressed in some hepatocellular carcinoma (at protein level).1 Publication

Developmental stagei

Its expression is closely related to cellular differentiation in both normal and malignant squamous cells.

Inductioni

Strongly up-regulated in the upper epidermis of sun-exposed skin.1 Publication

Gene expression databases

BgeeiENSG00000057149
GenevisibleiP29508 HS

Organism-specific databases

HPAiCAB018772
CAB036006
CAB036007
HPA048341
HPA049988
HPA055992

Interactioni

Subunit structurei

Interacts with MAPK8/JNK1.1 Publication

GO - Molecular functioni

  • protease binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112223, 54 interactors
IntActiP29508, 9 interactors
MINTiP29508
STRINGi9606.ENSP00000283752

Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 19Combined sources16
Beta strandi27 – 29Combined sources3
Helixi31 – 42Combined sources12
Helixi47 – 56Combined sources10
Helixi59 – 61Combined sources3
Helixi81 – 91Combined sources11
Beta strandi97 – 110Combined sources14
Helixi117 – 127Combined sources11
Beta strandi130 – 134Combined sources5
Turni136 – 138Combined sources3
Helixi140 – 153Combined sources14
Turni154 – 157Combined sources4
Turni165 – 167Combined sources3
Beta strandi174 – 188Combined sources15
Helixi192 – 194Combined sources3
Beta strandi196 – 203Combined sources8
Beta strandi206 – 224Combined sources19
Turni225 – 228Combined sources4
Beta strandi229 – 236Combined sources8
Beta strandi239 – 250Combined sources12
Turni251 – 253Combined sources3
Helixi254 – 260Combined sources7
Helixi263 – 269Combined sources7
Helixi272 – 274Combined sources3
Beta strandi276 – 285Combined sources10
Beta strandi287 – 294Combined sources8
Helixi296 – 302Combined sources7
Helixi306 – 308Combined sources3
Helixi315 – 318Combined sources4
Beta strandi319 – 321Combined sources3
Beta strandi324 – 336Combined sources13
Beta strandi338 – 352Combined sources15
Beta strandi361 – 364Combined sources4
Beta strandi369 – 375Combined sources7
Turni376 – 379Combined sources4
Beta strandi380 – 387Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZV6X-ray2.70A/B/C2-390[»]
4ZK0X-ray2.15A1-390[»]
4ZK3X-ray2.00A1-390[»]
ProteinModelPortaliP29508
SMRiP29508
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29508

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family. Ov-serpin subfamily.Curated

Phylogenomic databases

eggNOGiKOG2392 Eukaryota
COG4826 LUCA
GeneTreeiENSGT00760000118789
HOVERGENiHBG005957
InParanoidiP29508
KOiK13963
OMAiTNAYELK
PhylomeDBiP29508
TreeFamiTF352619

Family and domain databases

InterProiView protein in InterPro
IPR023795 Serpin_CS
IPR023796 Serpin_dom
IPR000215 Serpin_fam
IPR036186 Serpin_sf
PANTHERiPTHR11461 PTHR11461, 1 hit
PfamiView protein in Pfam
PF00079 Serpin, 1 hit
SMARTiView protein in SMART
SM00093 SERPIN, 1 hit
SUPFAMiSSF56574 SSF56574, 1 hit
PROSITEiView protein in PROSITE
PS00284 SERPIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P29508-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSLSEANTK FMFDLFQQFR KSKENNIFYS PISITSALGM VLLGAKDNTA
60 70 80 90 100
QQIKKVLHFD QVTENTTGKA ATYHVDRSGN VHHQFQKLLT EFNKSTDAYE
110 120 130 140 150
LKIANKLFGE KTYLFLQEYL DAIKKFYQTS VESVDFANAP EESRKKINSW
160 170 180 190 200
VESQTNEKIK NLIPEGNIGS NTTLVLVNAI YFKGQWEKKF NKEDTKEEKF
210 220 230 240 250
WPNKNTYKSI QMMRQYTSFH FASLEDVQAK VLEIPYKGKD LSMIVLLPNE
260 270 280 290 300
IDGLQKLEEK LTAEKLMEWT SLQNMRETRV DLHLPRFKVE ESYDLKDTLR
310 320 330 340 350
TMGMVDIFNG DADLSGMTGS RGLVLSGVLH KAFVEVTEEG AEAAAATAVV
360 370 380 390
GFGSSPTSTN EEFHCNHPFL FFIRQNKTNS ILFYGRFSSP
Length:390
Mass (Da):44,565
Last modified:January 23, 2002 - v2
Checksum:iE5F27F986C752CFA
GO
Isoform 2 (identifier: P29508-2) [UniParc]FASTAAdd to basket
Also known as: SCCA1b

The sequence of this isoform differs from the canonical sequence as follows:
     205-256: Missing.

Show »
Length:338
Mass (Da):38,550
Checksum:i5AA4782FF0C1ABDF
GO

Sequence cautioni

The sequence AAO11731 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16F → S in CAD56658 (PubMed:12975381).Curated1
Sequence conflicti47D → N in CAD56658 (PubMed:12975381).Curated1
Sequence conflicti105N → T in CAD56658 (PubMed:12975381).Curated1
Sequence conflicti185Q → R in AAO92272 (Ref. 5) Curated1
Sequence conflicti202P → S in AAO92272 (Ref. 5) Curated1
Sequence conflicti278T → A in AAO92269 (Ref. 5) Curated1
Sequence conflicti349V → E in AAO92272 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_024351351G → A Increased antiprotease activity and increased MAPK8 inhibition activity. 4 PublicationsCorresponds to variant dbSNP:rs3180227Ensembl.1
Natural variantiVAR_024352357T → A3 PublicationsCorresponds to variant dbSNP:rs1065205Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032657205 – 256Missing in isoform 2. 1 PublicationAdd BLAST52

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66896 mRNA Translation: AAB20405.1
U19556 mRNA Translation: AAA97552.1
U19568
, U19559, U19560, U19562, U19565, U19567 Genomic DNA Translation: AAA86317.1
U19562, U19559, U19560 Genomic DNA Translation: AAA86316.1
AB046399 mRNA Translation: BAB40772.1
AJ515706 mRNA Translation: CAD56658.1
AY245778 mRNA Translation: AAO92269.1
AY245781 mRNA Translation: AAO92272.1
EU852041 mRNA Translation: ACF21012.1
BT006748 mRNA Translation: AAP35394.1
AK222746 mRNA Translation: BAD96466.1
AK222753 mRNA Translation: BAD96473.1
AK314805 mRNA Translation: BAG37332.1
AC069356 Genomic DNA No translation available.
CH471096 Genomic DNA Translation: EAW63156.1
CH471096 Genomic DNA Translation: EAW63157.1
BC005224 mRNA Translation: AAH05224.1
AY190327 mRNA Translation: AAO11731.1 Different initiation.
CCDSiCCDS11987.1 [P29508-1]
PIRiI38201
RefSeqiNP_008850.1, NM_006919.2 [P29508-1]
UniGeneiHs.227948

Genome annotation databases

EnsembliENST00000283752; ENSP00000283752; ENSG00000057149 [P29508-1]
ENST00000332821; ENSP00000329498; ENSG00000057149 [P29508-2]
GeneIDi6317
KEGGihsa:6317
UCSCiuc002lji.3 human [P29508-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSPB3_HUMAN
AccessioniPrimary (citable) accession number: P29508
Secondary accession number(s): A6NDM2
, B2RBT5, B3W5Y6, Q53H28, Q53YB5, Q86VF3, Q86W04, Q8IWL4, Q8IXI3, Q96J21, Q9BYF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2002
Last modified: March 28, 2018
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health