P29499 (MT1_HOMAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Metallothionein-1 Alternative name(s): CuMT-1
Organism	Homarus americanus (American lobster)
Taxonomic identifier	6706 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Astacidea › Nephropoidea › Nephropidae › Homarus

Protein attributes

Sequence length	58 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals. The different forms of lobster metallothioneins may have different biological functions. Class I MTS in marine crustacea are involved in the sequestration of elevated levels of heavy-metal ions. Binds 6 metal ions. Known to bind cadmium.
Sequence similarities	Belongs to the metallothionein superfamily. Type 3 family.

Ontologies

Keywords
Ligand	Cadmium Copper Metal-binding Metal-thiolate cluster
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 58

58

Metallothionein-1

PRO_0000197342

Regions

Region

1 – 28

28

Beta

Region

29 – 58

30

Alpha

Sites

Metal binding

4

1

Divalent metal cation; cluster B

Metal binding

5

1

Divalent metal cation; cluster B

Metal binding

9

1

Divalent metal cation; cluster B

Metal binding

11

1

Divalent metal cation; cluster B

Metal binding

16

1

Divalent metal cation; cluster B

Metal binding

20

1

Divalent metal cation; cluster B

Metal binding

22

1

Divalent metal cation; cluster B

Metal binding

25

1

Divalent metal cation; cluster B

Metal binding

27

1

Divalent metal cation; cluster B

Metal binding

30

1

Divalent metal cation; cluster A

Metal binding

33

1

Divalent metal cation; cluster A

Metal binding

37

1

Divalent metal cation; cluster A

Metal binding

39

1

Divalent metal cation; cluster A

Metal binding

45

1

Divalent metal cation; cluster A

Metal binding

49

1

Divalent metal cation; cluster A

Metal binding

53

1

Divalent metal cation; cluster A

Metal binding

55

1

Divalent metal cation; cluster A

Metal binding

56

1

Divalent metal cation; cluster A

Secondary structure

1

.

.

.

.

.

.

.

.

.

58

Helix Strand Turn

Details...

Beta strand

6 – 8

3

Beta strand

11 – 14

4

Helix

31 – 33

3

Helix

45 – 48

4

Sequences

	Sequence		Length	Mass (Da)
	P29499 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 176ABAAF60A32F96	FASTA	58	5,977
	10 20 30 40 50 PGPCCKDKCE CAEGGCKTGC KCTSCRCAPC EKCTSGCKCP SKDECAKTCS KPCSCCXX

References

[1]	"Structural and functional diversity of copper-metallothioneins from the American lobster Homarus americanus." Brouwer M., Winge D.R., Gray W.R. J. Inorg. Biochem. 35:289-303(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Hepatopancreas.
[2]	"Sequential proton resonance assignments and metal cluster topology of lobster metallothionein-1." Zhu Z., Derose E.F., Mullen G.P., Petering D.H., Shaw C.F. III Biochemistry 33:8858-8865(1994) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

A37039.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J5L	NMR	-	A	29-56	[»]
1J5M	NMR	-	A	1-28	[»]

ProteinModelPortal

P29499.

SMR

P29499. Positions 1-28.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR002045. Metalthion_3_crustacean.
IPR017854. Metalthion_dom.
IPR003019. Metalthion_sfam_euk.
[Graphical view]

Pfam

PF00131. Metallothio. 1 hit.
[Graphical view]

PRINTS

PR00858. MTCRUSTACEAN.

SUPFAM

SSF57868. Metallothionein_sfam. 2 hits.

ProtoNet

Search...

Other

EvolutionaryTrace

P29499.

Entry information

Entry name

MT1_HOMAM

Accession

Primary (citable) accession number: P29499

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)