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Protein

Metallothionein-1

Gene
N/A
Organism
Homarus americanus (American lobster)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals. The different forms of lobster metallothioneins may have different biological functions. Class I MTS in marine crustacea are involved in the sequestration of elevated levels of heavy-metal ions. Binds 6 metal ions. Known to bind cadmium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi4 – 41Divalent metal cation; cluster B
Metal bindingi5 – 51Divalent metal cation; cluster B
Metal bindingi9 – 91Divalent metal cation; cluster B
Metal bindingi11 – 111Divalent metal cation; cluster B
Metal bindingi16 – 161Divalent metal cation; cluster B
Metal bindingi20 – 201Divalent metal cation; cluster B
Metal bindingi22 – 221Divalent metal cation; cluster B
Metal bindingi25 – 251Divalent metal cation; cluster B
Metal bindingi27 – 271Divalent metal cation; cluster B
Metal bindingi30 – 301Divalent metal cation; cluster A
Metal bindingi33 – 331Divalent metal cation; cluster A
Metal bindingi37 – 371Divalent metal cation; cluster A
Metal bindingi39 – 391Divalent metal cation; cluster A
Metal bindingi45 – 451Divalent metal cation; cluster A
Metal bindingi49 – 491Divalent metal cation; cluster A
Metal bindingi53 – 531Divalent metal cation; cluster A
Metal bindingi55 – 551Divalent metal cation; cluster A
Metal bindingi56 – 561Divalent metal cation; cluster A

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Cadmium, Copper, Metal-binding, Metal-thiolate cluster

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-1
Alternative name(s):
CuMT-1
OrganismiHomarus americanus (American lobster)
Taxonomic identifieri6706 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMalacostracaEumalacostracaEucaridaDecapodaPleocyemataAstacideaNephropoideaNephropidaeHomarus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5858Metallothionein-1PRO_0000197342Add
BLAST

Structurei

Secondary structure

1
58
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83Combined sources
Beta strandi11 – 144Combined sources
Helixi31 – 333Combined sources
Helixi45 – 484Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J5LNMR-A29-56[»]
1J5MNMR-A1-28[»]
ProteinModelPortaliP29499.
SMRiP29499. Positions 1-28.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29499.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2828BetaAdd
BLAST
Regioni29 – 5830AlphaAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR002045. Metalthion_3_crustacean.
IPR017854. Metalthion_dom.
IPR003019. Metalthion_sfam_euk.
[Graphical view]
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00858. MTCRUSTACEAN.
SUPFAMiSSF57868. SSF57868. 2 hits.

Sequencei

Sequence statusi: Complete.

P29499-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
PGPCCKDKCE CAEGGCKTGC KCTSCRCAPC EKCTSGCKCP SKDECAKTCS

KPCSCCXX
Length:58
Mass (Da):5,977
Last modified:November 1, 1997 - v2
Checksum:i176ABAAF60A32F96
GO

Sequence databases

PIRiA37039.

Cross-referencesi

Sequence databases

PIRiA37039.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J5LNMR-A29-56[»]
1J5MNMR-A1-28[»]
ProteinModelPortaliP29499.
SMRiP29499. Positions 1-28.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP29499.

Family and domain databases

InterProiIPR002045. Metalthion_3_crustacean.
IPR017854. Metalthion_dom.
IPR003019. Metalthion_sfam_euk.
[Graphical view]
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00858. MTCRUSTACEAN.
SUPFAMiSSF57868. SSF57868. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Structural and functional diversity of copper-metallothioneins from the American lobster Homarus americanus."
    Brouwer M., Winge D.R., Gray W.R.
    J. Inorg. Biochem. 35:289-303(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Hepatopancreas.
  2. "Sequential proton resonance assignments and metal cluster topology of lobster metallothionein-1."
    Zhu Z., Derose E.F., Mullen G.P., Petering D.H., Shaw C.F. III
    Biochemistry 33:8858-8865(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiMT1_HOMAM
AccessioniPrimary (citable) accession number: P29499
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.