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Protein

Minichromosome maintenance protein 5

Gene

MCM5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity.2 Publications

Miscellaneous

Present with 10300 molecules/cell in log phase SD medium.1 Publication
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological active complex.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi416 – 423ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: SGD
  • DNA replication origin binding Source: SGD
  • helicase activity Source: UniProtKB-KW

GO - Biological processi

  • chromatin silencing at telomere Source: SGD
  • DNA duplex unwinding Source: GOC
  • DNA replication initiation Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • establishment of chromatin silencing Source: SGD
  • negative regulation of chromatin silencing at telomere Source: SGD
  • nuclear DNA replication Source: SGD
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
  • regulation of DNA-dependent DNA replication initiation Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32373-MONOMER
ReactomeiR-SCE-68949 Orc1 removal from chromatin
R-SCE-68962 Activation of the pre-replicative complex
R-SCE-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
Minichromosome maintenance protein 5 (EC:3.6.4.12)
Alternative name(s):
Cell division control protein 46
Gene namesi
Name:MCM5
Synonyms:CDC46
Ordered Locus Names:YLR274W
ORF Names:L9328.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR274W
SGDiS000004264 MCM5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi422K → A: Loss of MCM2-7 complex helicase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941111 – 775Minichromosome maintenance protein 5Add BLAST775

Proteomic databases

MaxQBiP29496
PaxDbiP29496
PRIDEiP29496

PTM databases

iPTMnetiP29496

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5; loaded onto DNA, forms a head-head double hexamer. Interacts with CSM1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31542, 254 interactors
DIPiDIP-2406N
IntActiP29496, 81 interactors
MINTiP29496
STRINGi4932.YLR274W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JA8electron microscopy3.8051-775[»]
3JC5electron microscopy4.7051-775[»]
3JC6electron microscopy3.7051-775[»]
3JC7electron microscopy4.8051-775[»]
5BK4electron microscopy3.905/D1-775[»]
5H7Ielectron microscopy7.1051-775[»]
5U8Selectron microscopy6.1051-775[»]
5U8Telectron microscopy4.9051-775[»]
5UDBelectron microscopy3.9051-775[»]
5V8Felectron microscopy3.9051-775[»]
5XF8electron microscopy7.1051-775[»]
6F0Lelectron microscopy4.775/D1-775[»]
ProteinModelPortaliP29496
SMRiP29496
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini366 – 573MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi548 – 551Arginine finger4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi351 – 356Poly-Glu6

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074928
HOGENOMiHOG000224128
InParanoidiP29496
KOiK02209
OMAiIHKVLQL
OrthoDBiEOG092C0VUM

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008048 MCM5
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01661 MCMPROTEIN5
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 2 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P29496-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFDRPEIYS APVLQGESPN DDDNTEIIKS FKNFILEFRL DSQFIYRDQL
60 70 80 90 100
RNNILVKNYS LTVNMEHLIG YNEDIYKKLS DEPSDIIPLF ETAITQVAKR
110 120 130 140 150
ISILSRAQSA NNNDKDPENT SMDTDSLLLN SLPTFQLILN SNANQIPLRD
160 170 180 190 200
LDSEHVSKIV RLSGIIISTS VLSSRATYLS IMCRNCRHTT SITINNFNSI
210 220 230 240 250
TGNTVSLPRS CLSTIESESS MANESNIGDE STKKNCGPDP YIIIHESSKF
260 270 280 290 300
IDQQFLKLQE IPELVPVGEM PRNLTMTCDR YLTNKVIPGT RVTIVGIYSI
310 320 330 340 350
YNSKNGAGSG RSGGGNGGSG VAIRTPYIKI LGIQSDVETS SIWNSVTMFT
360 370 380 390 400
EEEEEEFLQL SRNPKLYEIL TNSIAPSIFG NEDIKKAIVC LLMGGSKKIL
410 420 430 440 450
PDGMRLRGDI NVLLLGDPGT AKSQLLKFVE KVSPIAVYTS GKGSSAAGLT
460 470 480 490 500
ASVQRDPMTR EFYLEGGAMV LADGGVVCID EFDKMRDEDR VAIHEAMEQQ
510 520 530 540 550
TISIAKAGIT TVLNSRTSVL AAANPIYGRY DDLKSPGDNI DFQTTILSRF
560 570 580 590 600
DMIFIVKDDH NEERDISIAN HVINIHTGNA NAMQNQQEEN GSEISIEKMK
610 620 630 640 650
RYITYCRLKC APRLSPQAAE KLSSNFVTIR KQLLINELES TERSSIPITI
660 670 680 690 700
RQLEAIIRIT ESLAKLELSP IAQERHVDEA IRLFQASTMD AASQDPIGGL
710 720 730 740 750
NQASGTSLSE IRRFEQELKR RLPIGWSTSY QTLRREFVDT HRFSQLALDK
760 770
ALYALEKHET IQLRHQGQNI YRSGV
Length:775
Mass (Da):86,411
Last modified:April 1, 1993 - v1
Checksum:i01D9DE208A091CF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09242 Unassigned DNA Translation: AAA18027.1
U17245 Genomic DNA Translation: AAB67364.1
BK006945 Genomic DNA Translation: DAA09587.1
PIRiA39631
RefSeqiNP_013376.1, NM_001182161.1

Genome annotation databases

EnsemblFungiiYLR274W; YLR274W; YLR274W
GeneIDi850980
KEGGisce:YLR274W

Similar proteinsi

Entry informationi

Entry nameiMCM5_YEAST
AccessioniPrimary (citable) accession number: P29496
Secondary accession number(s): D6VYS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
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Main funding by: National Institutes of Health