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P29478

- SEC65_YEAST

UniProt

P29478 - SEC65_YEAST

Protein

Signal recognition particle subunit SEC65

Gene

SEC65

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SEC65 is required for SRP integrity.

    GO - Molecular functioni

    1. 7S RNA binding Source: SGD

    GO - Biological processi

    1. protein targeting to ER Source: SGD
    2. SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32689-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal recognition particle subunit SEC65
    Gene namesi
    Name:SEC65
    Ordered Locus Names:YML105C
    ORF Names:YM8339.14C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML105c.
    SGDiS000004573. SEC65.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. signal recognition particle, endoplasmic reticulum targeting Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Signal recognition particle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 273273Signal recognition particle subunit SEC65PRO_0000135212Add
    BLAST

    Proteomic databases

    MaxQBiP29478.
    PaxDbiP29478.
    PeptideAtlasiP29478.
    PRIDEiP29478.

    Expressioni

    Gene expression databases

    GenevestigatoriP29478.

    Interactioni

    Subunit structurei

    Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14.1 Publication

    Protein-protein interaction databases

    BioGridi35038. 35 interactions.
    DIPiDIP-3850N.
    IntActiP29478. 5 interactions.
    MINTiMINT-506803.
    STRINGi4932.YML105C.

    Structurei

    3D structure databases

    ProteinModelPortaliP29478.
    SMRiP29478. Positions 100-168.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG1400.
    HOGENOMiHOG000248333.
    KOiK03105.
    OMAiDIDNMDM.
    OrthoDBiEOG7W6WZX.

    Family and domain databases

    Gene3Di3.30.56.30. 1 hit.
    HAMAPiMF_00305. SRP19.
    InterProiIPR002778. Signal_recog_particle_SRP19.
    IPR022938. SRP19_arc-type.
    [Graphical view]
    PANTHERiPTHR17453. PTHR17453. 1 hit.
    PfamiPF01922. SRP19. 1 hit.
    [Graphical view]
    SUPFAMiSSF69695. SSF69695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P29478-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRLEEIDDF NDIDDLDMEL AELDPSLRTP IAPKITPKVV RSQDQENPAF    50
    LPGTNNNSNS NNNSSNEKEQ LSFINPKTGK VERSEAISKK DLEEVKRFQV 100
    LYPCYFDINR SHKEGRRVPK ELAVENPLAK TMADAVRELG ILCIFEGEKC 150
    HPQDFGNPGR IRVLFKENGQ LIGAATKFKG GKRQLMKAVG EYMKRHPTTI 200
    ESLREIPYGP DFDNIEFKKI PRVKGFKMNE IVPLHSPFLM GHPMTKSVYE 250
    TPKITAAEKS FKPPKNKYKV VRR 273
    Length:273
    Mass (Da):31,170
    Last modified:April 1, 1993 - v1
    Checksum:i4F4A6CBB9225E385
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65783 Genomic DNA. Translation: CAA46666.1.
    Z49210 Genomic DNA. Translation: CAA89113.1.
    AY693145 Genomic DNA. Translation: AAT93164.1.
    BK006946 Genomic DNA. Translation: DAA09793.1.
    PIRiS21731.
    RefSeqiNP_013602.1. NM_001182467.1.

    Genome annotation databases

    EnsemblFungiiYML105C; YML105C; YML105C.
    GeneIDi854866.
    KEGGisce:YML105C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65783 Genomic DNA. Translation: CAA46666.1 .
    Z49210 Genomic DNA. Translation: CAA89113.1 .
    AY693145 Genomic DNA. Translation: AAT93164.1 .
    BK006946 Genomic DNA. Translation: DAA09793.1 .
    PIRi S21731.
    RefSeqi NP_013602.1. NM_001182467.1.

    3D structure databases

    ProteinModelPortali P29478.
    SMRi P29478. Positions 100-168.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35038. 35 interactions.
    DIPi DIP-3850N.
    IntActi P29478. 5 interactions.
    MINTi MINT-506803.
    STRINGi 4932.YML105C.

    Proteomic databases

    MaxQBi P29478.
    PaxDbi P29478.
    PeptideAtlasi P29478.
    PRIDEi P29478.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML105C ; YML105C ; YML105C .
    GeneIDi 854866.
    KEGGi sce:YML105C.

    Organism-specific databases

    CYGDi YML105c.
    SGDi S000004573. SEC65.

    Phylogenomic databases

    eggNOGi COG1400.
    HOGENOMi HOG000248333.
    KOi K03105.
    OMAi DIDNMDM.
    OrthoDBi EOG7W6WZX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32689-MONOMER.

    Miscellaneous databases

    NextBioi 977792.
    PROi P29478.

    Gene expression databases

    Genevestigatori P29478.

    Family and domain databases

    Gene3Di 3.30.56.30. 1 hit.
    HAMAPi MF_00305. SRP19.
    InterProi IPR002778. Signal_recog_particle_SRP19.
    IPR022938. SRP19_arc-type.
    [Graphical view ]
    PANTHERi PTHR17453. PTHR17453. 1 hit.
    Pfami PF01922. SRP19. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69695. SSF69695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19."
      Stirling C.J., Hewitt E.W.
      Nature 356:534-537(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity."
      Hann B.C., Stirling C.J., Walter P.
      Nature 356:532-533(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression."
      Brown J.D., Hann B.C., Medzihradszky K.F., Niwa M., Burlingame A.L., Walter P.
      EMBO J. 13:4390-4400(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SRP COMPLEX.

    Entry informationi

    Entry nameiSEC65_YEAST
    AccessioniPrimary (citable) accession number: P29478
    Secondary accession number(s): D6W0H9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3