Reviewed, UniProtKB/Swiss-Prot P29478 (SEC65_YEAST)

Names and origin

Protein names

Recommended name:
    Signal recognition particle subunit SEC65

Gene names

Name:	SEC65
Ordered Locus Names:	YML105C
ORF Names:	YM8339.14C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SEC65 is required for SRP integrity.
Subunit structure	Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Cellular component	Cytoplasm Signal recognition particle
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition Ref.4 Inferred from mutant phenotype. Source: SGD
Cellular component	signal recognition particle, endoplasmic reticulum targeting Ref.1 Ref.4 Inferred from direct assay. Source: SGD
Molecular function	7S RNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Signal recognition particle subunit SEC65		PRO_0000135212
Amino acid modifications
	Modified residue	36	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P29478-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 4F4A6CBB9225E385	FASTA	273	31,170
	10 20 30 40 50 60 MPRLEEIDDF NDIDDLDMEL AELDPSLRTP IAPKITPKVV RSQDQENPAF LPGTNNNSNS 70 80 90 100 110 120 NNNSSNEKEQ LSFINPKTGK VERSEAISKK DLEEVKRFQV LYPCYFDINR SHKEGRRVPK 130 140 150 160 170 180 ELAVENPLAK TMADAVRELG ILCIFEGEKC HPQDFGNPGR IRVLFKENGQ LIGAATKFKG 190 200 210 220 230 240 GKRQLMKAVG EYMKRHPTTI ESLREIPYGP DFDNIEFKKI PRVKGFKMNE IVPLHSPFLM 250 260 270 GHPMTKSVYE TPKITAAEKS FKPPKNKYKV VRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19." Stirling C.J., Hewitt E.W. Nature 356:534-537(1992) [PubMed: 1313948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity." Hann B.C., Stirling C.J., Walter P. Nature 356:532-533(1992) [PubMed: 1313947] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression." Brown J.D., Hann B.C., Medzihradszky K.F., Niwa M., Burlingame A.L., Walter P. EMBO J. 13:4390-4400(1994) [PubMed: 7925282] [Abstract] Cited for: IDENTIFICATION IN THE SRP COMPLEX.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-36, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65783 Genomic DNA. Translation: CAA46666.1. Z49210 Genomic DNA. Translation: CAA89113.1. AY693145 Genomic DNA. Translation: AAT93164.1.
PIR	S21731.
RefSeq	NP_013602.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3850N.
IntAct	P29478. 10 interactions.
STRING	P29478.
Proteomic databases
PeptideAtlas	P29478.
Genome annotation databases
Ensembl	YML105C; YML105C; YML105C; Saccharomyces cerevisiae. [Genome view]
GeneID	854866.
KEGG	sce:YML105C.
NMPDR	fig|4932.3.peg.4638.
Organism-specific databases
CYGD	YML105c.
SGD	S000004573. SEC65.
Phylogenomic databases
HOGENOM	P29478.
OMA	PQDFGNP
OrthoDB	EOG98GXKF
Gene expression databases
ArrayExpress	P29478.
Genevestigator	P29478.
GermOnline	YML105C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002778. Signal_recog_particle_SRP19. [Graphical view]
Gene3D	G3DSA:3.30.56.30. SRP19. 1 hit.
PANTHER	PTHR17453. SRP19. 1 hit.
Pfam	PF01922. SRP19. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977792.

Entry information

Entry name

SEC65_YEAST

Accession

Primary (citable) accession number: P29478

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 24, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)