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P29478

- SEC65_YEAST

UniProt

P29478 - SEC65_YEAST

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Protein

Signal recognition particle subunit SEC65

Gene

SEC65

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SEC65 is required for SRP integrity.

GO - Molecular functioni

  1. 7S RNA binding Source: SGD

GO - Biological processi

  1. protein targeting to ER Source: SGD
  2. SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Enzyme and pathway databases

BioCyciYEAST:G3O-32689-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle subunit SEC65
Gene namesi
Name:SEC65
Ordered Locus Names:YML105C
ORF Names:YM8339.14C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML105c.
SGDiS000004573. SEC65.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. signal recognition particle, endoplasmic reticulum targeting Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Signal recognition particle subunit SEC65PRO_0000135212Add
BLAST

Proteomic databases

MaxQBiP29478.
PaxDbiP29478.
PeptideAtlasiP29478.
PRIDEiP29478.

Expressioni

Gene expression databases

GenevestigatoriP29478.

Interactioni

Subunit structurei

Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14.1 Publication

Protein-protein interaction databases

BioGridi35038. 36 interactions.
DIPiDIP-3850N.
IntActiP29478. 5 interactions.
MINTiMINT-506803.
STRINGi4932.YML105C.

Structurei

3D structure databases

ProteinModelPortaliP29478.
SMRiP29478. Positions 100-168.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1400.
HOGENOMiHOG000248333.
InParanoidiP29478.
KOiK03105.
OMAiDIDNMDM.
OrthoDBiEOG7W6WZX.

Family and domain databases

Gene3Di3.30.56.30. 1 hit.
HAMAPiMF_00305. SRP19.
InterProiIPR002778. Signal_recog_particle_SRP19.
IPR022938. SRP19_arc-type.
[Graphical view]
PANTHERiPTHR17453. PTHR17453. 1 hit.
PfamiPF01922. SRP19. 1 hit.
[Graphical view]
SUPFAMiSSF69695. SSF69695. 1 hit.

Sequencei

Sequence statusi: Complete.

P29478-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPRLEEIDDF NDIDDLDMEL AELDPSLRTP IAPKITPKVV RSQDQENPAF
60 70 80 90 100
LPGTNNNSNS NNNSSNEKEQ LSFINPKTGK VERSEAISKK DLEEVKRFQV
110 120 130 140 150
LYPCYFDINR SHKEGRRVPK ELAVENPLAK TMADAVRELG ILCIFEGEKC
160 170 180 190 200
HPQDFGNPGR IRVLFKENGQ LIGAATKFKG GKRQLMKAVG EYMKRHPTTI
210 220 230 240 250
ESLREIPYGP DFDNIEFKKI PRVKGFKMNE IVPLHSPFLM GHPMTKSVYE
260 270
TPKITAAEKS FKPPKNKYKV VRR
Length:273
Mass (Da):31,170
Last modified:April 1, 1993 - v1
Checksum:i4F4A6CBB9225E385
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65783 Genomic DNA. Translation: CAA46666.1.
Z49210 Genomic DNA. Translation: CAA89113.1.
AY693145 Genomic DNA. Translation: AAT93164.1.
BK006946 Genomic DNA. Translation: DAA09793.1.
PIRiS21731.
RefSeqiNP_013602.1. NM_001182467.1.

Genome annotation databases

EnsemblFungiiYML105C; YML105C; YML105C.
GeneIDi854866.
KEGGisce:YML105C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X65783 Genomic DNA. Translation: CAA46666.1 .
Z49210 Genomic DNA. Translation: CAA89113.1 .
AY693145 Genomic DNA. Translation: AAT93164.1 .
BK006946 Genomic DNA. Translation: DAA09793.1 .
PIRi S21731.
RefSeqi NP_013602.1. NM_001182467.1.

3D structure databases

ProteinModelPortali P29478.
SMRi P29478. Positions 100-168.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35038. 36 interactions.
DIPi DIP-3850N.
IntActi P29478. 5 interactions.
MINTi MINT-506803.
STRINGi 4932.YML105C.

Proteomic databases

MaxQBi P29478.
PaxDbi P29478.
PeptideAtlasi P29478.
PRIDEi P29478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML105C ; YML105C ; YML105C .
GeneIDi 854866.
KEGGi sce:YML105C.

Organism-specific databases

CYGDi YML105c.
SGDi S000004573. SEC65.

Phylogenomic databases

eggNOGi COG1400.
HOGENOMi HOG000248333.
InParanoidi P29478.
KOi K03105.
OMAi DIDNMDM.
OrthoDBi EOG7W6WZX.

Enzyme and pathway databases

BioCyci YEAST:G3O-32689-MONOMER.

Miscellaneous databases

NextBioi 977792.
PROi P29478.

Gene expression databases

Genevestigatori P29478.

Family and domain databases

Gene3Di 3.30.56.30. 1 hit.
HAMAPi MF_00305. SRP19.
InterProi IPR002778. Signal_recog_particle_SRP19.
IPR022938. SRP19_arc-type.
[Graphical view ]
PANTHERi PTHR17453. PTHR17453. 1 hit.
Pfami PF01922. SRP19. 1 hit.
[Graphical view ]
SUPFAMi SSF69695. SSF69695. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19."
    Stirling C.J., Hewitt E.W.
    Nature 356:534-537(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity."
    Hann B.C., Stirling C.J., Walter P.
    Nature 356:532-533(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression."
    Brown J.D., Hann B.C., Medzihradszky K.F., Niwa M., Burlingame A.L., Walter P.
    EMBO J. 13:4390-4400(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SRP COMPLEX.

Entry informationi

Entry nameiSEC65_YEAST
AccessioniPrimary (citable) accession number: P29478
Secondary accession number(s): D6W0H9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3