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P29478 (SEC65_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal recognition particle subunit SEC65
Gene names
Name:SEC65
Ordered Locus Names:YML105C
ORF Names:YM8339.14C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SEC65 is required for SRP integrity. HAMAP-Rule MF_00305

Subunit structure

Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14. Ref.6

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00305.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273Signal recognition particle subunit SEC65 HAMAP-Rule MF_00305
PRO_0000135212

Sequences

Sequence LengthMass (Da)Tools
P29478 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 4F4A6CBB9225E385

FASTA27331,170
        10         20         30         40         50         60 
MPRLEEIDDF NDIDDLDMEL AELDPSLRTP IAPKITPKVV RSQDQENPAF LPGTNNNSNS 

        70         80         90        100        110        120 
NNNSSNEKEQ LSFINPKTGK VERSEAISKK DLEEVKRFQV LYPCYFDINR SHKEGRRVPK 

       130        140        150        160        170        180 
ELAVENPLAK TMADAVRELG ILCIFEGEKC HPQDFGNPGR IRVLFKENGQ LIGAATKFKG 

       190        200        210        220        230        240 
GKRQLMKAVG EYMKRHPTTI ESLREIPYGP DFDNIEFKKI PRVKGFKMNE IVPLHSPFLM 

       250        260        270 
GHPMTKSVYE TPKITAAEKS FKPPKNKYKV VRR 

« Hide

References

« Hide 'large scale' references
[1]"The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19."
Stirling C.J., Hewitt E.W.
Nature 356:534-537(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity."
Hann B.C., Stirling C.J., Walter P.
Nature 356:532-533(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression."
Brown J.D., Hann B.C., Medzihradszky K.F., Niwa M., Burlingame A.L., Walter P.
EMBO J. 13:4390-4400(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE SRP COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65783 Genomic DNA. Translation: CAA46666.1.
Z49210 Genomic DNA. Translation: CAA89113.1.
AY693145 Genomic DNA. Translation: AAT93164.1.
BK006946 Genomic DNA. Translation: DAA09793.1.
PIRS21731.
RefSeqNP_013602.1. NM_001182467.1.

3D structure databases

ProteinModelPortalP29478.
SMRP29478. Positions 99-195.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35038. 35 interactions.
DIPDIP-3850N.
IntActP29478. 5 interactions.
MINTMINT-506803.
STRING4932.YML105C.

Proteomic databases

PaxDbP29478.
PeptideAtlasP29478.
PRIDEP29478.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML105C; YML105C; YML105C.
GeneID854866.
KEGGsce:YML105C.

Organism-specific databases

CYGDYML105c.
SGDS000004573. SEC65.

Phylogenomic databases

eggNOGCOG1400.
HOGENOMHOG000248333.
KOK03105.
OMAVKGFKMN.
OrthoDBEOG7W6WZX.

Enzyme and pathway databases

BioCycYEAST:G3O-32689-MONOMER.

Gene expression databases

GenevestigatorP29478.

Family and domain databases

Gene3D3.30.56.30. 1 hit.
HAMAPMF_00305. SRP19.
InterProIPR002778. Signal_recog_particle_SRP19.
IPR022938. SRP19_arc-type.
[Graphical view]
PANTHERPTHR17453. PTHR17453. 1 hit.
PfamPF01922. SRP19. 1 hit.
[Graphical view]
SUPFAMSSF69695. SSF69695. 1 hit.
ProtoNetSearch...

Other

NextBio977792.
PROP29478.

Entry information

Entry nameSEC65_YEAST
AccessionPrimary (citable) accession number: P29478
Secondary accession number(s): D6W0H9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD