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P29465

- CHS3_YEAST

UniProt

P29465 - CHS3_YEAST

Protein

Chitin synthase 3

Gene

CHS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Appears to be responsible for the synthesis of the majority of the chitin found in the cell wall periphery. It is involved in the synthesis of the chitin ring that forms in the cell wall just before bud emergence. This ring remains at the base of the bud as the bud grows and ultimately forms part of the bud scar marking the division site on the mother cell.

    Catalytic activityi

    UDP-N-acetyl-alpha-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).

    GO - Molecular functioni

    1. chitin synthase activity Source: SGD

    GO - Biological processi

    1. ascospore wall assembly Source: SGD
    2. fungal-type cell wall chitin biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciYEAST:YBR023C-MONOMER.
    BRENDAi2.4.1.16. 984.

    Protein family/group databases

    CAZyiGT2. Glycosyltransferase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chitin synthase 3 (EC:2.4.1.16)
    Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase 3
    Class-IV chitin synthase 3
    Gene namesi
    Name:CHS3
    Synonyms:CAL1, CSD2, DIT101, KIT2
    Ordered Locus Names:YBR023C
    ORF Names:YBR0305
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR023c.
    SGDiS000000227. CHS3.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. chitosome Source: SGD
    3. cytoplasm Source: SGD
    4. incipient cellular bud site Source: SGD
    5. integral component of membrane Source: UniProtKB-KW
    6. prospore membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11651165Chitin synthase 3PRO_0000193729Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki136 – 136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Modified residuei537 – 5371Phosphoserine1 Publication
    Modified residuei538 – 5381Phosphothreonine1 Publication

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP29465.
    PaxDbiP29465.
    PeptideAtlasiP29465.

    Expressioni

    Gene expression databases

    GenevestigatoriP29465.

    Interactioni

    Subunit structurei

    Seems to interact with BNI4 and SKT5 which link CHS3 to septins.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CHS7P388431EBI-4632,EBI-24756
    DNF1P326601EBI-4632,EBI-3121
    DRS2P395241EBI-4632,EBI-3106
    DTR1P381251EBI-4632,EBI-20904
    FLC1Q089671EBI-4632,EBI-36673
    FLC2P397191EBI-4632,EBI-20703
    KEX1P096201EBI-4632,EBI-9653
    KEX2P131341EBI-4632,EBI-9658
    PIN2Q120571EBI-4632,EBI-29838
    RET2P436211EBI-4632,EBI-4876

    Protein-protein interaction databases

    BioGridi32726. 174 interactions.
    DIPiDIP-2482N.
    IntActiP29465. 19 interactions.
    MINTiMINT-1363219.
    STRINGi4932.YBR023C.

    Structurei

    3D structure databases

    ProteinModelPortaliP29465.
    SMRiP29465. Positions 235-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 170170ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini192 – 20211CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini224 – 452229ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini474 – 1006533CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1028 – 10292ExtracellularSequence Analysis
    Topological domaini1051 – 10555CytoplasmicSequence Analysis
    Topological domaini1077 – 10804ExtracellularSequence Analysis
    Topological domaini1102 – 116564CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei171 – 19121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei203 – 22321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei453 – 47321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1007 – 102721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1030 – 105021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1056 – 107621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1081 – 110121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1215.
    GeneTreeiENSGT00530000064569.
    HOGENOMiHOG000158449.
    KOiK00698.
    OMAiTELEFEM.
    OrthoDBiEOG7V76FQ.

    Family and domain databases

    InterProiIPR004835. Chitin_synth.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR22914. PTHR22914. 1 hit.
    SUPFAMiSSF53448. SSF53448. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P29465-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGLNGDDPD DYYLNLNQDE ESLLRSRHSV GSGAPHRQGS LVRPERSRLN     50
    NPDNPHFYYA QKTQEQMNHL DVLPSSTGVN PNATRRSGSL RSKGSVRSKF 100
    SGRETDSYLL QDMNTTDKKA SVKISDEGVA EDEFDKDGDV DNFEESSTQP 150
    INKSIKPLRK ETNDTLSFWQ MYCYFITFWA PAPILAFCGM PKKERQMAWR 200
    EKVALISVIL YIGAIVAFLT FGFTKTVCSS SKLRLKNNEV STEFVVINGK 250
    AYELDTSSRS GIQDVEVDSD TLYGPWSDAG KDASFLFQNV NGNCHNLITP 300
    KSNSSIPHDD DNNLAWYFPC KLKNQDGSSK PNFTVENYAG WNCHTSKEDR 350
    DAFYGLKSKA DVYFTWDGIK NSSRNLIVYN GDVLDLDLLD WLEKDDVDYP 400
    VVFDDLKTSN LQGYDLSLVL SNGHERKIAR CLSEIIKVGE VDSKTVGCIA 450
    SDVVLYVSLV FILSVVIIKF IIACYFRWTV ARKQGAYIVD NKTMDKHTND 500
    IEDWSNNIQT KAPLKEVDPH LRPKKYSKKS LGHKRASTFD LLKKHSSKMF 550
    QFNESVIDLD TSMSSSLQSS GSYRGMTTMT TQNAWKLSNE NKAVHSRNPS 600
    TLLPTSSMFW NKATSSPVPG SSLIQSLDST IIHPDIVQQP PLDFMPYGFP 650
    LIHTICFVTC YSEDEEGLRT TLDSLSTTDY PNSHKLLMVV CDGLIKGSGN 700
    DKTTPEIALG MMDDFVTPPD EVKPYSYVAV ASGSKRHNMA KIYAGFYKYD 750
    DSTIPPENQQ RVPIITIVKC GTPAEQGAAK PGNRGKRDSQ IILMSFLEKI 800
    TFDERMTQLE FQLLKNIWQI TGLMADFYET VLMVDADTKV FPDALTHMVA 850
    EMVKDPLIMG LCGETKIANK AQSWVTAIQV FEYYISHHQA KAFESVFGSV 900
    TCLPGCFSMY RIKSPKGSDG YWVPVLANPD IVERYSDNVT NTLHKKNLLL 950
    LGEDRFLSSL MLKTFPKRKQ VFVPKAACKT IAPDKFKVLL SQRRRWINST 1000
    VHNLFELVLI RDLCGTFCFS MQFVIGIELI GTMVLPLAIC FTIYVIIFAI 1050
    VSKPTPVITL VLLAIILGLP GLIVVITATR WSYLWWMCVY ICALPIWNFV 1100
    LPSYAYWKFD DFSWGDTRTI AGGNKKAQDE NEGEFDHSKI KMRTWREFER 1150
    EDILNRKEES DSFVA 1165
    Length:1,165
    Mass (Da):131,601
    Last modified:October 1, 1994 - v3
    Checksum:iEBF9227DC30D3EA4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1163 – 11631F → L in AAA34844. (PubMed:1532231)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76078 Genomic DNA. Translation: CAA53680.1.
    M73697 Genomic DNA. Translation: AAA34844.1.
    Z35892 Genomic DNA. Translation: CAA84965.1.
    X57300 Genomic DNA. Translation: CAA40559.1.
    BK006936 Genomic DNA. Translation: DAA07145.1.
    PIRiS45879.
    RefSeqiNP_009579.1. NM_001178371.1.

    Genome annotation databases

    EnsemblFungiiYBR023C; YBR023C; YBR023C.
    GeneIDi852311.
    KEGGisce:YBR023C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76078 Genomic DNA. Translation: CAA53680.1 .
    M73697 Genomic DNA. Translation: AAA34844.1 .
    Z35892 Genomic DNA. Translation: CAA84965.1 .
    X57300 Genomic DNA. Translation: CAA40559.1 .
    BK006936 Genomic DNA. Translation: DAA07145.1 .
    PIRi S45879.
    RefSeqi NP_009579.1. NM_001178371.1.

    3D structure databases

    ProteinModelPortali P29465.
    SMRi P29465. Positions 235-290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32726. 174 interactions.
    DIPi DIP-2482N.
    IntActi P29465. 19 interactions.
    MINTi MINT-1363219.
    STRINGi 4932.YBR023C.

    Protein family/group databases

    CAZyi GT2. Glycosyltransferase Family 2.

    Proteomic databases

    MaxQBi P29465.
    PaxDbi P29465.
    PeptideAtlasi P29465.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR023C ; YBR023C ; YBR023C .
    GeneIDi 852311.
    KEGGi sce:YBR023C.

    Organism-specific databases

    CYGDi YBR023c.
    SGDi S000000227. CHS3.

    Phylogenomic databases

    eggNOGi COG1215.
    GeneTreei ENSGT00530000064569.
    HOGENOMi HOG000158449.
    KOi K00698.
    OMAi TELEFEM.
    OrthoDBi EOG7V76FQ.

    Enzyme and pathway databases

    BioCyci YEAST:YBR023C-MONOMER.
    BRENDAi 2.4.1.16. 984.

    Miscellaneous databases

    NextBioi 970993.

    Gene expression databases

    Genevestigatori P29465.

    Family and domain databases

    InterProi IPR004835. Chitin_synth.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR22914. PTHR22914. 1 hit.
    SUPFAMi SSF53448. SSF53448. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: the CSD2 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis."
      Bulawa C.E.
      Mol. Cell. Biol. 12:1764-1776(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
      Smits P.H.M., de Haan M., Maat C., Grivell L.A.
      Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae."
      Valdivieso M.H., Mol P.C., Shaw J.A., Cabib E., Duran A.
      J. Cell Biol. 114:101-109(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-1165.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-136.
      Strain: SUB592.
    8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537 AND THR-538, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCHS3_YEAST
    AccessioniPrimary (citable) accession number: P29465
    Secondary accession number(s): D6VQ25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1510 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3