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P29465

- CHS3_YEAST

UniProt

P29465 - CHS3_YEAST

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Protein

Chitin synthase 3

Gene

CHS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Appears to be responsible for the synthesis of the majority of the chitin found in the cell wall periphery. It is involved in the synthesis of the chitin ring that forms in the cell wall just before bud emergence. This ring remains at the base of the bud as the bud grows and ultimately forms part of the bud scar marking the division site on the mother cell.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).

GO - Molecular functioni

  1. chitin synthase activity Source: SGD

GO - Biological processi

  1. ascospore wall assembly Source: SGD
  2. fungal-type cell wall chitin biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:YBR023C-MONOMER.
BRENDAi2.4.1.16. 984.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitin synthase 3 (EC:2.4.1.16)
Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 3
Class-IV chitin synthase 3
Gene namesi
Name:CHS3
Synonyms:CAL1, CSD2, DIT101, KIT2
Ordered Locus Names:YBR023C
ORF Names:YBR0305
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR023c.
SGDiS000000227. CHS3.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. chitosome Source: SGD
  3. cytoplasm Source: SGD
  4. incipient cellular bud site Source: SGD
  5. integral component of membrane Source: UniProtKB-KW
  6. prospore membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11651165Chitin synthase 3PRO_0000193729Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki136 – 136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei537 – 5371Phosphoserine1 Publication
Modified residuei538 – 5381Phosphothreonine1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP29465.
PaxDbiP29465.
PeptideAtlasiP29465.

Expressioni

Gene expression databases

GenevestigatoriP29465.

Interactioni

Subunit structurei

Seems to interact with BNI4 and SKT5 which link CHS3 to septins.

Binary interactionsi

WithEntry#Exp.IntActNotes
CHS7P388431EBI-4632,EBI-24756
DNF1P326601EBI-4632,EBI-3121
DRS2P395241EBI-4632,EBI-3106
DTR1P381251EBI-4632,EBI-20904
FLC1Q089671EBI-4632,EBI-36673
FLC2P397191EBI-4632,EBI-20703
KEX1P096201EBI-4632,EBI-9653
KEX2P131341EBI-4632,EBI-9658
PIN2Q120571EBI-4632,EBI-29838
RET2P436211EBI-4632,EBI-4876

Protein-protein interaction databases

BioGridi32726. 174 interactions.
DIPiDIP-2482N.
IntActiP29465. 19 interactions.
MINTiMINT-1363219.
STRINGi4932.YBR023C.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4U9TX-ray2.59P10-28[»]
ProteinModelPortaliP29465.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 170170ExtracellularSequence AnalysisAdd
BLAST
Topological domaini192 – 20211CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini224 – 452229ExtracellularSequence AnalysisAdd
BLAST
Topological domaini474 – 1006533CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini1028 – 10292ExtracellularSequence Analysis
Topological domaini1051 – 10555CytoplasmicSequence Analysis
Topological domaini1077 – 10804ExtracellularSequence Analysis
Topological domaini1102 – 116564CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei171 – 19121HelicalSequence AnalysisAdd
BLAST
Transmembranei203 – 22321HelicalSequence AnalysisAdd
BLAST
Transmembranei453 – 47321HelicalSequence AnalysisAdd
BLAST
Transmembranei1007 – 102721HelicalSequence AnalysisAdd
BLAST
Transmembranei1030 – 105021HelicalSequence AnalysisAdd
BLAST
Transmembranei1056 – 107621HelicalSequence AnalysisAdd
BLAST
Transmembranei1081 – 110121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00530000064569.
HOGENOMiHOG000158449.
InParanoidiP29465.
KOiK00698.
OMAiTELEFEM.
OrthoDBiEOG7V76FQ.

Family and domain databases

InterProiIPR004835. Chitin_synth.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22914. PTHR22914. 1 hit.
SUPFAMiSSF53448. SSF53448. 3 hits.

Sequencei

Sequence statusi: Complete.

P29465-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGLNGDDPD DYYLNLNQDE ESLLRSRHSV GSGAPHRQGS LVRPERSRLN
60 70 80 90 100
NPDNPHFYYA QKTQEQMNHL DVLPSSTGVN PNATRRSGSL RSKGSVRSKF
110 120 130 140 150
SGRETDSYLL QDMNTTDKKA SVKISDEGVA EDEFDKDGDV DNFEESSTQP
160 170 180 190 200
INKSIKPLRK ETNDTLSFWQ MYCYFITFWA PAPILAFCGM PKKERQMAWR
210 220 230 240 250
EKVALISVIL YIGAIVAFLT FGFTKTVCSS SKLRLKNNEV STEFVVINGK
260 270 280 290 300
AYELDTSSRS GIQDVEVDSD TLYGPWSDAG KDASFLFQNV NGNCHNLITP
310 320 330 340 350
KSNSSIPHDD DNNLAWYFPC KLKNQDGSSK PNFTVENYAG WNCHTSKEDR
360 370 380 390 400
DAFYGLKSKA DVYFTWDGIK NSSRNLIVYN GDVLDLDLLD WLEKDDVDYP
410 420 430 440 450
VVFDDLKTSN LQGYDLSLVL SNGHERKIAR CLSEIIKVGE VDSKTVGCIA
460 470 480 490 500
SDVVLYVSLV FILSVVIIKF IIACYFRWTV ARKQGAYIVD NKTMDKHTND
510 520 530 540 550
IEDWSNNIQT KAPLKEVDPH LRPKKYSKKS LGHKRASTFD LLKKHSSKMF
560 570 580 590 600
QFNESVIDLD TSMSSSLQSS GSYRGMTTMT TQNAWKLSNE NKAVHSRNPS
610 620 630 640 650
TLLPTSSMFW NKATSSPVPG SSLIQSLDST IIHPDIVQQP PLDFMPYGFP
660 670 680 690 700
LIHTICFVTC YSEDEEGLRT TLDSLSTTDY PNSHKLLMVV CDGLIKGSGN
710 720 730 740 750
DKTTPEIALG MMDDFVTPPD EVKPYSYVAV ASGSKRHNMA KIYAGFYKYD
760 770 780 790 800
DSTIPPENQQ RVPIITIVKC GTPAEQGAAK PGNRGKRDSQ IILMSFLEKI
810 820 830 840 850
TFDERMTQLE FQLLKNIWQI TGLMADFYET VLMVDADTKV FPDALTHMVA
860 870 880 890 900
EMVKDPLIMG LCGETKIANK AQSWVTAIQV FEYYISHHQA KAFESVFGSV
910 920 930 940 950
TCLPGCFSMY RIKSPKGSDG YWVPVLANPD IVERYSDNVT NTLHKKNLLL
960 970 980 990 1000
LGEDRFLSSL MLKTFPKRKQ VFVPKAACKT IAPDKFKVLL SQRRRWINST
1010 1020 1030 1040 1050
VHNLFELVLI RDLCGTFCFS MQFVIGIELI GTMVLPLAIC FTIYVIIFAI
1060 1070 1080 1090 1100
VSKPTPVITL VLLAIILGLP GLIVVITATR WSYLWWMCVY ICALPIWNFV
1110 1120 1130 1140 1150
LPSYAYWKFD DFSWGDTRTI AGGNKKAQDE NEGEFDHSKI KMRTWREFER
1160
EDILNRKEES DSFVA
Length:1,165
Mass (Da):131,601
Last modified:October 1, 1994 - v3
Checksum:iEBF9227DC30D3EA4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1163 – 11631F → L in AAA34844. (PubMed:1532231)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76078 Genomic DNA. Translation: CAA53680.1.
M73697 Genomic DNA. Translation: AAA34844.1.
Z35892 Genomic DNA. Translation: CAA84965.1.
X57300 Genomic DNA. Translation: CAA40559.1.
BK006936 Genomic DNA. Translation: DAA07145.1.
PIRiS45879.
RefSeqiNP_009579.1. NM_001178371.1.

Genome annotation databases

EnsemblFungiiYBR023C; YBR023C; YBR023C.
GeneIDi852311.
KEGGisce:YBR023C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76078 Genomic DNA. Translation: CAA53680.1 .
M73697 Genomic DNA. Translation: AAA34844.1 .
Z35892 Genomic DNA. Translation: CAA84965.1 .
X57300 Genomic DNA. Translation: CAA40559.1 .
BK006936 Genomic DNA. Translation: DAA07145.1 .
PIRi S45879.
RefSeqi NP_009579.1. NM_001178371.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4U9T X-ray 2.59 P 10-28 [» ]
ProteinModelPortali P29465.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32726. 174 interactions.
DIPi DIP-2482N.
IntActi P29465. 19 interactions.
MINTi MINT-1363219.
STRINGi 4932.YBR023C.

Chemistry

ChEMBLi CHEMBL5597.

Protein family/group databases

CAZyi GT2. Glycosyltransferase Family 2.

Proteomic databases

MaxQBi P29465.
PaxDbi P29465.
PeptideAtlasi P29465.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR023C ; YBR023C ; YBR023C .
GeneIDi 852311.
KEGGi sce:YBR023C.

Organism-specific databases

CYGDi YBR023c.
SGDi S000000227. CHS3.

Phylogenomic databases

eggNOGi COG1215.
GeneTreei ENSGT00530000064569.
HOGENOMi HOG000158449.
InParanoidi P29465.
KOi K00698.
OMAi TELEFEM.
OrthoDBi EOG7V76FQ.

Enzyme and pathway databases

BioCyci YEAST:YBR023C-MONOMER.
BRENDAi 2.4.1.16. 984.

Miscellaneous databases

NextBioi 970993.

Gene expression databases

Genevestigatori P29465.

Family and domain databases

InterProi IPR004835. Chitin_synth.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR22914. PTHR22914. 1 hit.
SUPFAMi SSF53448. SSF53448. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: the CSD2 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis."
    Bulawa C.E.
    Mol. Cell. Biol. 12:1764-1776(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
    Smits P.H.M., de Haan M., Maat C., Grivell L.A.
    Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae."
    Valdivieso M.H., Mol P.C., Shaw J.A., Cabib E., Duran A.
    J. Cell Biol. 114:101-109(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-1165.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-136.
    Strain: SUB592.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537 AND THR-538, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCHS3_YEAST
AccessioniPrimary (citable) accession number: P29465
Secondary accession number(s): D6VQ25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1510 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3