P29465 (CHS3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 3
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 3
Class-IV chitin synthase 3

Gene names

Name:	CHS3
Synonyms:	CAL1, CSD2, DIT101, KIT2
Ordered Locus Names:	YBR023C
ORF Names:	YBR0305

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1165 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to be responsible for the synthesis of the majority of the chitin found in the cell wall periphery. It is involved in the synthesis of the chitin ring that forms in the cell wall just before bud emergence. This ring remains at the base of the bud as the bud grows and ultimately forms part of the bud scar marking the division site on the mother cell.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subunit structure	Seems to interact with BNI4 and SKT5 which link CHS3 to septins.
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	Present with 1510 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the chitin synthase family. Class IV subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ascospore wall assembly Inferred from mutant phenotype. Source: SGD cytokinesis Traceable author statement. Source: SGD fungal-type cell wall chitin biosynthetic process Traceable author statement. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD chitosome Inferred from direct assay. Source: SGD incipient cellular bud site Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mating projection tip Inferred from direct assay. Source: SGD prospore membrane Inferred from direct assay. Source: SGD
Molecular function	chitin synthase activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CHS7	P38843	1	EBI-4632,EBI-24756
DNF1	P32660	1	EBI-4632,EBI-3121
DRS2	P39524	1	EBI-4632,EBI-3106
DTR1	P38125	1	EBI-4632,EBI-20904
FLC1	Q08967	1	EBI-4632,EBI-36673
FLC2	P39719	1	EBI-4632,EBI-20703
KEX1	P09620	1	EBI-4632,EBI-9653
KEX2	P13134	1	EBI-4632,EBI-9658
PIN2	Q12057	1	EBI-4632,EBI-29838
RET2	P43621	1	EBI-4632,EBI-4876

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1165	1165	Chitin synthase 3		PRO_0000193729
Regions
	Topological domain	1 – 170	170	Extracellular Potential
	Transmembrane	171 – 191	21	Helical; Potential
	Topological domain	192 – 202	11	Cytoplasmic Potential
	Transmembrane	203 – 223	21	Helical; Potential
	Topological domain	224 – 452	229	Extracellular Potential
	Transmembrane	453 – 473	21	Helical; Potential
	Topological domain	474 – 1006	533	Cytoplasmic Potential
	Transmembrane	1007 – 1027	21	Helical; Potential
	Topological domain	1028 – 1029	2	Extracellular Potential
	Transmembrane	1030 – 1050	21	Helical; Potential
	Topological domain	1051 – 1055	5	Cytoplasmic Potential
	Transmembrane	1056 – 1076	21	Helical; Potential
	Topological domain	1077 – 1080	4	Extracellular Potential
	Transmembrane	1081 – 1101	21	Helical; Potential
	Topological domain	1102 – 1165	64	Cytoplasmic Potential
Amino acid modifications
	Modified residue	147	1	Phosphoserine Ref.10 Ref.11
	Modified residue	537	1	Phosphoserine Ref.9
	Modified residue	538	1	Phosphothreonine Ref.9
	Modified residue	606	1	Phosphoserine Ref.7
	Cross-link	136		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7
Experimental info
	Sequence conflict	1163	1	F → L in AAA34844. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29465 [UniParc]. Last modified October 1, 1994. Version 3. Checksum: EBF9227DC30D3EA4	FASTA	1,165	131,601
	10 20 30 40 50 60 MTGLNGDDPD DYYLNLNQDE ESLLRSRHSV GSGAPHRQGS LVRPERSRLN NPDNPHFYYA 70 80 90 100 110 120 QKTQEQMNHL DVLPSSTGVN PNATRRSGSL RSKGSVRSKF SGRETDSYLL QDMNTTDKKA 130 140 150 160 170 180 SVKISDEGVA EDEFDKDGDV DNFEESSTQP INKSIKPLRK ETNDTLSFWQ MYCYFITFWA 190 200 210 220 230 240 PAPILAFCGM PKKERQMAWR EKVALISVIL YIGAIVAFLT FGFTKTVCSS SKLRLKNNEV 250 260 270 280 290 300 STEFVVINGK AYELDTSSRS GIQDVEVDSD TLYGPWSDAG KDASFLFQNV NGNCHNLITP 310 320 330 340 350 360 KSNSSIPHDD DNNLAWYFPC KLKNQDGSSK PNFTVENYAG WNCHTSKEDR DAFYGLKSKA 370 380 390 400 410 420 DVYFTWDGIK NSSRNLIVYN GDVLDLDLLD WLEKDDVDYP VVFDDLKTSN LQGYDLSLVL 430 440 450 460 470 480 SNGHERKIAR CLSEIIKVGE VDSKTVGCIA SDVVLYVSLV FILSVVIIKF IIACYFRWTV 490 500 510 520 530 540 ARKQGAYIVD NKTMDKHTND IEDWSNNIQT KAPLKEVDPH LRPKKYSKKS LGHKRASTFD 550 560 570 580 590 600 LLKKHSSKMF QFNESVIDLD TSMSSSLQSS GSYRGMTTMT TQNAWKLSNE NKAVHSRNPS 610 620 630 640 650 660 TLLPTSSMFW NKATSSPVPG SSLIQSLDST IIHPDIVQQP PLDFMPYGFP LIHTICFVTC 670 680 690 700 710 720 YSEDEEGLRT TLDSLSTTDY PNSHKLLMVV CDGLIKGSGN DKTTPEIALG MMDDFVTPPD 730 740 750 760 770 780 EVKPYSYVAV ASGSKRHNMA KIYAGFYKYD DSTIPPENQQ RVPIITIVKC GTPAEQGAAK 790 800 810 820 830 840 PGNRGKRDSQ IILMSFLEKI TFDERMTQLE FQLLKNIWQI TGLMADFYET VLMVDADTKV 850 860 870 880 890 900 FPDALTHMVA EMVKDPLIMG LCGETKIANK AQSWVTAIQV FEYYISHHQA KAFESVFGSV 910 920 930 940 950 960 TCLPGCFSMY RIKSPKGSDG YWVPVLANPD IVERYSDNVT NTLHKKNLLL LGEDRFLSSL 970 980 990 1000 1010 1020 MLKTFPKRKQ VFVPKAACKT IAPDKFKVLL SQRRRWINST VHNLFELVLI RDLCGTFCFS 1030 1040 1050 1060 1070 1080 MQFVIGIELI GTMVLPLAIC FTIYVIIFAI VSKPTPVITL VLLAIILGLP GLIVVITATR 1090 1100 1110 1120 1130 1140 WSYLWWMCVY ICALPIWNFV LPSYAYWKFD DFSWGDTRTI AGGNKKAQDE NEGEFDHSKI 1150 1160 KMRTWREFER EDILNRKEES DSFVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: the CSD2 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis." Bulawa C.E. Mol. Cell. Biol. 12:1764-1776(1992) [PubMed: 1532231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene." Smits P.H.M., de Haan M., Maat C., Grivell L.A. Yeast 10:S75-S80(1994) [PubMed: 8091864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae." Valdivieso M.H., Mol P.C., Shaw J.A., Cabib E., Duran A. J. Cell Biol. 114:101-109(1991) [PubMed: 2050737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-1165.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-606, UBIQUITINATION AT LYS-136, MASS SPECTROMETRY. Strain: SUB592.
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-537 AND THR-538, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-147, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-147, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76078 Genomic DNA. Translation: CAA53680.1. M73697 Genomic DNA. Translation: AAA34844.1. Z35892 Genomic DNA. Translation: CAA84965.1. X57300 Genomic DNA. Translation: CAA40559.1. BK006936 Genomic DNA. Translation: DAA07145.1.
PIR	S45879.
RefSeq	NP_009579.1. NM_001178371.1.
3D structure databases
ProteinModelPortal	P29465.
SMR	P29465. Positions 235-290.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2482N.
IntAct	P29465. 12 interactions.
MINT	MINT-1363219.
STRING	P29465.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Proteomic databases
PeptideAtlas	P29465.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR023C; YBR023C; YBR023C.
GeneID	852311.
KEGG	sce:YBR023C.
NMPDR	fig|4932.3.peg.274.
Organism-specific databases
CYGD	YBR023c.
SGD	S000000227. CHS3.
Phylogenomic databases
eggNOG	fuNOG06113.
GeneTree	EFGT00050000001909.
HOGENOM	HBG333864.
OMA	DTKVFPD.
OrthoDB	EOG4KD9V4.
Enzyme and pathway databases
BRENDA	2.4.1.16. 984.
Gene expression databases
ArrayExpress	P29465.
Genevestigator	P29465.
GermOnline	YBR023C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. [Graphical view]
KO	K00698.
Pfam	PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970993.

Entry information

Entry name

CHS3_YEAST

Accession

Primary (citable) accession number: P29465
Secondary accession number(s): D6VQ25

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program