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P29461

- PTP2_YEAST

UniProt

P29461 - PTP2_YEAST

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Protein

Tyrosine-protein phosphatase 2

Gene

PTP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Major phosphatase responsible with PTP3 for tyrosine dephosphorylation of MAP kinase HOG1 to inactivate its activity. May also be involved in the regulation of MAP kinase FUS3. May be implicated in the ubiquitin-mediated protein degradation.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei666 – 6661Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: SGD

GO - Biological processi

  1. inactivation of MAPK activity involved in cell wall organization or biogenesis Source: SGD
  2. inactivation of MAPK activity involved in osmosensory signaling pathway Source: SGD
  3. peptidyl-tyrosine dephosphorylation Source: GOC
  4. protein dephosphorylation Source: SGD
  5. regulation of protein localization Source: SGD
  6. regulation of sporulation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciYEAST:YOR208W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase 2 (EC:3.1.3.48)
Alternative name(s):
Protein-tyrosine phosphatase 2
Short name:
PTPase 2
Gene namesi
Name:PTP2
Ordered Locus Names:YOR208W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR208w.
SGDiS000005734. PTP2.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Tyrosine-protein phosphatase 2PRO_0000094856Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei258 – 2581Phosphoserine1 Publication
Modified residuei430 – 4301Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP29461.
PaxDbiP29461.

Expressioni

Gene expression databases

GenevestigatoriP29461.

Interactioni

Subunit structurei

Interacts with HOG1.2 Publications

Protein-protein interaction databases

BioGridi34603. 48 interactions.
DIPiDIP-2369N.
IntActiP29461. 3 interactions.
MINTiMINT-510066.
STRINGi4932.YOR208W.

Structurei

3D structure databases

ProteinModelPortaliP29461.
SMRiP29461. Positions 375-738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini383 – 737355Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00690000103379.
HOGENOMiHOG000115780.
InParanoidiP29461.
KOiK01104.
OrthoDBiEOG793BJ4.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 3 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29461-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDRIAQQYRN GKRDNNGNRM ASSAISEKGH IQVNQTRTPG QMPVYRGETI
60 70 80 90 100
NLSNLPQNQI KPCKDLDDVN IRRNNSNRHS KILLLDLCAG PNTNSFLGNT
110 120 130 140 150
NAKDITVLSL PLPSTLVKRS NYPFENLLKN YLGSDEKYIE FTKIIKDYDI
160 170 180 190 200
FIFSDSFSRI SSCLKTTFCL IEKFKKFICH FFPSPYLKFF LLEGSLNDSK
210 220 230 240 250
APSLGKNKKN CILPKLDLNL NVNLTSRSTL NLRINIPPPN DSNKIFLQSL
260 270 280 290 300
KKDLIHYSPN SLQKFFQFNM PADLAPNDTI LPNWLKFCSV KENEKVILKK
310 320 330 340 350
LFNNFETLEN FEMQRLEKCL KFKKKPLHQK QLSQKQRGPQ STDDSKLYSL
360 370 380 390 400
TSLQRQYKSS LKSNIQKNQK LKLIIPKNNT SSSPSPLSSD DTIMSPINDY
410 420 430 440 450
ELTEGIQSFT KNRYSNILPY EHSRVKLPHS PKPPAVSEAS TTETKTDKSY
460 470 480 490 500
PMCPVDAKNH SCKPNDYINA NYLKLTQINP DFKYIATQAP LPSTMDDFWK
510 520 530 540 550
VITLNKVKVI ISLNSDDELN LRKWDIYWNN LSYSNHTIKL QNTWENICNI
560 570 580 590 600
NGCVLRVFQV KKTAPQNDNI SQDCDLPHNG DLTSITMAVS EPFIVYQLQY
610 620 630 640 650
KNWLDSCGVD MNDIIKLHKV KNSLLFNPQS FITSLEKDVC KPDLIDDNNS
660 670 680 690 700
ELHLDTANSS PLLVHCSAGC GRTGVFVTLD FLLSILSPTT NHSNKIDVWN
710 720 730 740 750
MTQDLIFIIV NELRKQRISM VQNLTQYIAC YEALLNYFAL QKQIKNALPC
Length:750
Mass (Da):85,868
Last modified:April 1, 1993 - v1
Checksum:i1033D2F0AA23BD35
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti371 – 3711L → S in AAB59323. (PubMed:1452018)Curated
Sequence conflicti474 – 4752KL → NV(PubMed:1577774)Curated
Sequence conflicti660 – 6612SP → GA in AAB59323. (PubMed:1452018)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85287 Genomic DNA. No translation available.
M82872 Genomic DNA. Translation: AAA34922.1.
M38723 Genomic DNA. Translation: AAB59323.1.
Z75116 Genomic DNA. Translation: CAA99423.1.
BK006948 Genomic DNA. Translation: DAA10980.1.
PIRiS67100.
RefSeqiNP_014851.3. NM_001183627.3.

Genome annotation databases

EnsemblFungiiYOR208W; YOR208W; YOR208W.
GeneIDi854383.
KEGGisce:YOR208W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85287 Genomic DNA. No translation available.
M82872 Genomic DNA. Translation: AAA34922.1 .
M38723 Genomic DNA. Translation: AAB59323.1 .
Z75116 Genomic DNA. Translation: CAA99423.1 .
BK006948 Genomic DNA. Translation: DAA10980.1 .
PIRi S67100.
RefSeqi NP_014851.3. NM_001183627.3.

3D structure databases

ProteinModelPortali P29461.
SMRi P29461. Positions 375-738.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34603. 48 interactions.
DIPi DIP-2369N.
IntActi P29461. 3 interactions.
MINTi MINT-510066.
STRINGi 4932.YOR208W.

Proteomic databases

MaxQBi P29461.
PaxDbi P29461.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR208W ; YOR208W ; YOR208W .
GeneIDi 854383.
KEGGi sce:YOR208W.

Organism-specific databases

CYGDi YOR208w.
SGDi S000005734. PTP2.

Phylogenomic databases

eggNOGi COG5599.
GeneTreei ENSGT00690000103379.
HOGENOMi HOG000115780.
InParanoidi P29461.
KOi K01104.
OrthoDBi EOG793BJ4.

Enzyme and pathway databases

BioCyci YEAST:YOR208W-MONOMER.

Miscellaneous databases

NextBioi 976526.

Gene expression databases

Genevestigatori P29461.

Family and domain databases

Gene3Di 3.90.190.10. 1 hit.
InterProi IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view ]
Pfami PF00102. Y_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00700. PRTYPHPHTASE.
SMARTi SM00194. PTPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 3 hits.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a second protein tyrosine phosphatase gene, PTP2, from Saccharomyces cerevisiae."
    Guan K., Deschenes R.J., Dixon J.E.
    J. Biol. Chem. 267:10024-10030(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A gene encoding a putative tyrosine phosphatase suppresses lethality of an N-end rule-dependent mutant."
    Ota I.M., Varshavsky A.
    Proc. Natl. Acad. Sci. U.S.A. 89:2355-2359(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Multiple protein tyrosine phosphatase-encoding genes in the yeast Saccharomyces cerevisiae."
    James P., Hall B.D., Whelen S., Craig E.A.
    Gene 122:101-110(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Regulation of the Saccharomyces cerevisiae HOG1 mitogen-activated protein kinase by the PTP2 and PTP3 protein tyrosine phosphatases."
    Wurgler-Murphy S.M., Maeda T., Witten E.A., Saito H.
    Mol. Cell. Biol. 17:1289-1297(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HOG1.
  7. "Two protein-tyrosine phosphatases inactivate the osmotic stress response pathway in yeast by targeting the mitogen-activated protein kinase, Hog1."
    Jacoby T., Flanagan H., Faykin A., Seto A.G., Mattison C.P., Ota I.M.
    J. Biol. Chem. 272:17749-17755(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HOG1.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258 AND SER-430, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-430, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPTP2_YEAST
AccessioniPrimary (citable) accession number: P29461
Secondary accession number(s): D6W2R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3