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P29461

- PTP2_YEAST

UniProt

P29461 - PTP2_YEAST

Protein

Tyrosine-protein phosphatase 2

Gene

PTP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Major phosphatase responsible with PTP3 for tyrosine dephosphorylation of MAP kinase HOG1 to inactivate its activity. May also be involved in the regulation of MAP kinase FUS3. May be implicated in the ubiquitin-mediated protein degradation.2 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei666 – 6661Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: SGD

    GO - Biological processi

    1. inactivation of MAPK activity involved in cell wall organization or biogenesis Source: SGD
    2. inactivation of MAPK activity involved in osmosensory signaling pathway Source: SGD
    3. peptidyl-tyrosine dephosphorylation Source: GOC
    4. protein dephosphorylation Source: SGD
    5. regulation of protein localization Source: SGD
    6. regulation of sporulation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciYEAST:YOR208W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase 2 (EC:3.1.3.48)
    Alternative name(s):
    Protein-tyrosine phosphatase 2
    Short name:
    PTPase 2
    Gene namesi
    Name:PTP2
    Ordered Locus Names:YOR208W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR208w.
    SGDiS000005734. PTP2.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 750750Tyrosine-protein phosphatase 2PRO_0000094856Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei258 – 2581Phosphoserine1 Publication
    Modified residuei430 – 4301Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP29461.
    PaxDbiP29461.

    Expressioni

    Gene expression databases

    GenevestigatoriP29461.

    Interactioni

    Subunit structurei

    Interacts with HOG1.2 Publications

    Protein-protein interaction databases

    BioGridi34603. 48 interactions.
    DIPiDIP-2369N.
    IntActiP29461. 3 interactions.
    MINTiMINT-510066.
    STRINGi4932.YOR208W.

    Structurei

    3D structure databases

    ProteinModelPortaliP29461.
    SMRiP29461. Positions 375-738.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini383 – 737355Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5599.
    GeneTreeiENSGT00690000103379.
    HOGENOMiHOG000115780.
    KOiK01104.
    OrthoDBiEOG793BJ4.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 3 hits.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29461-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRIAQQYRN GKRDNNGNRM ASSAISEKGH IQVNQTRTPG QMPVYRGETI    50
    NLSNLPQNQI KPCKDLDDVN IRRNNSNRHS KILLLDLCAG PNTNSFLGNT 100
    NAKDITVLSL PLPSTLVKRS NYPFENLLKN YLGSDEKYIE FTKIIKDYDI 150
    FIFSDSFSRI SSCLKTTFCL IEKFKKFICH FFPSPYLKFF LLEGSLNDSK 200
    APSLGKNKKN CILPKLDLNL NVNLTSRSTL NLRINIPPPN DSNKIFLQSL 250
    KKDLIHYSPN SLQKFFQFNM PADLAPNDTI LPNWLKFCSV KENEKVILKK 300
    LFNNFETLEN FEMQRLEKCL KFKKKPLHQK QLSQKQRGPQ STDDSKLYSL 350
    TSLQRQYKSS LKSNIQKNQK LKLIIPKNNT SSSPSPLSSD DTIMSPINDY 400
    ELTEGIQSFT KNRYSNILPY EHSRVKLPHS PKPPAVSEAS TTETKTDKSY 450
    PMCPVDAKNH SCKPNDYINA NYLKLTQINP DFKYIATQAP LPSTMDDFWK 500
    VITLNKVKVI ISLNSDDELN LRKWDIYWNN LSYSNHTIKL QNTWENICNI 550
    NGCVLRVFQV KKTAPQNDNI SQDCDLPHNG DLTSITMAVS EPFIVYQLQY 600
    KNWLDSCGVD MNDIIKLHKV KNSLLFNPQS FITSLEKDVC KPDLIDDNNS 650
    ELHLDTANSS PLLVHCSAGC GRTGVFVTLD FLLSILSPTT NHSNKIDVWN 700
    MTQDLIFIIV NELRKQRISM VQNLTQYIAC YEALLNYFAL QKQIKNALPC 750
    Length:750
    Mass (Da):85,868
    Last modified:April 1, 1993 - v1
    Checksum:i1033D2F0AA23BD35
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti371 – 3711L → S in AAB59323. (PubMed:1452018)Curated
    Sequence conflicti474 – 4752KL → NV(PubMed:1577774)Curated
    Sequence conflicti660 – 6612SP → GA in AAB59323. (PubMed:1452018)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85287 Genomic DNA. No translation available.
    M82872 Genomic DNA. Translation: AAA34922.1.
    M38723 Genomic DNA. Translation: AAB59323.1.
    Z75116 Genomic DNA. Translation: CAA99423.1.
    BK006948 Genomic DNA. Translation: DAA10980.1.
    PIRiS67100.
    RefSeqiNP_014851.3. NM_001183627.3.

    Genome annotation databases

    EnsemblFungiiYOR208W; YOR208W; YOR208W.
    GeneIDi854383.
    KEGGisce:YOR208W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85287 Genomic DNA. No translation available.
    M82872 Genomic DNA. Translation: AAA34922.1 .
    M38723 Genomic DNA. Translation: AAB59323.1 .
    Z75116 Genomic DNA. Translation: CAA99423.1 .
    BK006948 Genomic DNA. Translation: DAA10980.1 .
    PIRi S67100.
    RefSeqi NP_014851.3. NM_001183627.3.

    3D structure databases

    ProteinModelPortali P29461.
    SMRi P29461. Positions 375-738.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34603. 48 interactions.
    DIPi DIP-2369N.
    IntActi P29461. 3 interactions.
    MINTi MINT-510066.
    STRINGi 4932.YOR208W.

    Proteomic databases

    MaxQBi P29461.
    PaxDbi P29461.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR208W ; YOR208W ; YOR208W .
    GeneIDi 854383.
    KEGGi sce:YOR208W.

    Organism-specific databases

    CYGDi YOR208w.
    SGDi S000005734. PTP2.

    Phylogenomic databases

    eggNOGi COG5599.
    GeneTreei ENSGT00690000103379.
    HOGENOMi HOG000115780.
    KOi K01104.
    OrthoDBi EOG793BJ4.

    Enzyme and pathway databases

    BioCyci YEAST:YOR208W-MONOMER.

    Miscellaneous databases

    NextBioi 976526.

    Gene expression databases

    Genevestigatori P29461.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 3 hits.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a second protein tyrosine phosphatase gene, PTP2, from Saccharomyces cerevisiae."
      Guan K., Deschenes R.J., Dixon J.E.
      J. Biol. Chem. 267:10024-10030(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A gene encoding a putative tyrosine phosphatase suppresses lethality of an N-end rule-dependent mutant."
      Ota I.M., Varshavsky A.
      Proc. Natl. Acad. Sci. U.S.A. 89:2355-2359(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Multiple protein tyrosine phosphatase-encoding genes in the yeast Saccharomyces cerevisiae."
      James P., Hall B.D., Whelen S., Craig E.A.
      Gene 122:101-110(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Regulation of the Saccharomyces cerevisiae HOG1 mitogen-activated protein kinase by the PTP2 and PTP3 protein tyrosine phosphatases."
      Wurgler-Murphy S.M., Maeda T., Witten E.A., Saito H.
      Mol. Cell. Biol. 17:1289-1297(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HOG1.
    7. "Two protein-tyrosine phosphatases inactivate the osmotic stress response pathway in yeast by targeting the mitogen-activated protein kinase, Hog1."
      Jacoby T., Flanagan H., Faykin A., Seto A.G., Mattison C.P., Ota I.M.
      J. Biol. Chem. 272:17749-17755(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HOG1.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258 AND SER-430, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-430, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPTP2_YEAST
    AccessioniPrimary (citable) accession number: P29461
    Secondary accession number(s): D6W2R4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 149 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3