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Protein

DNA replication licensing factor mcm4

Gene

mcm4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Required for S phase execution.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi545 – 552ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: PomBase
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: InterPro

GO - Biological processi

  • mitotic DNA replication initiation Source: PomBase
  • negative regulation of ATP-dependent DNA helicase activity Source: PomBase
  • premeiotic DNA replication Source: PomBase
  • replication fork protection Source: PomBase

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-68962 Activation of the pre-replicative complex
R-SPO-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor mcm4 (EC:3.6.4.12)
Alternative name(s):
Cell division control protein 21
Minichromosome maintenance protein 4
Gene namesi
Name:mcm4
Synonyms:cdc21
ORF Names:SPCC16A11.17, SPCC24B10.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

PomBaseiSPCC16A11.17 mcm4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941061 – 931DNA replication licensing factor mcm4Add BLAST931

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei37Phosphoserine1 Publication1
Modified residuei38Phosphoserine1 Publication1
Modified residuei41Phosphoserine1 Publication1
Modified residuei92Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP29458
PaxDbiP29458
PRIDEiP29458

PTM databases

iPTMnetiP29458

Interactioni

Subunit structurei

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimers of mcm4/mcm6 and mcm3/mcm5 interact with mcm2 and mcm7.Curated1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiP29458, 10 interactors
MINTiP29458
STRINGi4896.SPCC16A11.17.1

Structurei

3D structure databases

ProteinModelPortaliP29458
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini493 – 702MCMAdd BLAST210

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi677 – 680Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

HOGENOMiHOG000224127
InParanoidiP29458
KOiK02212
OrthoDBiEOG092C0VUM
PhylomeDBiP29458

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008047 MCM_4
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF66 PTHR11630:SF66, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01660 MCMPROTEIN4
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P29458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSQQSGRA NELRTPGRAN SSSREAVDSS PLFFPASSPG STRLTTPRTT
60 70 80 90 100
ARTPLASSPL LFESSSPGPN IPQSSRSHLL SQRNDLFLDS SSQRTPRSTR
110 120 130 140 150
RGDIHSSVQM STPSRRREVD PQRPGVSTPS SLLFSGSDAL TFSQAHPSSE
160 170 180 190 200
VADDTVRVIW GTNVSIQESI ASFRGFLRGF KKKYRPEYRN ELMPPPDAEQ
210 220 230 240 250
LVYIEALRNM RIMGLEILNL DVQDLKHYPP TKKLYHQLYS YPQEIIPIMD
260 270 280 290 300
QTIKDVMLDL LGTNPPEDVL NDIELKIYKI RPFNLEKCIN MRDLNPGDID
310 320 330 340 350
KLISIKGLVL RCTPVIPDMK QAFFRCSVCG HCVTVEIDRG RIAEPIKCPR
360 370 380 390 400
EVCGATNAMQ LIHNRSEFAD KQVIKLQETP DVVPDGQTPH SVSLCVYDEL
410 420 430 440 450
VDSARAGDRI EVTGIFRCVP VRLNPRMRTV KSLFKTYVDV VHIKKQDKRR
460 470 480 490 500
LGTDPSTLES DIAEDAALQI DEVRKISDEE VEKIQQVSKR DDIYDILSRS
510 520 530 540 550
LAPSIYEMDD VKKGLLLQLF GGTNKSFHKG ASPRYRGDIN ILMCGDPSTS
560 570 580 590 600
KSQILKYVHK IAPRGVYTSG KGSSAVGLTA YITRDQDTKQ LVLESGALVL
610 620 630 640 650
SDGGICCIDE FDKMSDATRS ILHEVMEQQT VTVAKAGIIT TLNARTSILA
660 670 680 690 700
SANPIGSKYN PDLPVTKNID LPPTLLSRFD LVYLILDRVD ETLDRKLANH
710 720 730 740 750
IVSMYMEDTP EHATDMEVFS VEFLTSYITY ARNNINPVIS EEAAKELVNA
760 770 780 790 800
YVGMRKLGED VRASEKRITA TTRQLESMIR LSEAHAKMHL RNVVEVGDVL
810 820 830 840 850
EAARLIKTAI KDYATDPATG KISLDLIYVN ERETLVPEDM VKELANLISN
860 870 880 890 900
LTVGGKTMLV SQLLTRFREQ SSTRLDASDF EACLGAFREQ SSTRLDASDF
910 920 930
EACLGALERR GRIKVITSAG HRIVRSIAQT D
Length:931
Mass (Da):103,728
Last modified:May 30, 2000 - v2
Checksum:iBCA9B045FC62811D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti872 – 891Missing (PubMed:11859360).CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58824 Genomic DNA Translation: CAA41628.1
CU329672 Genomic DNA Translation: CAB53089.1
PIRiS26640
RefSeqiNP_588004.2, NM_001022995.2

Genome annotation databases

GeneIDi2539164
KEGGispo:SPCC16A11.17

Similar proteinsi

Entry informationi

Entry nameiMCM4_SCHPO
AccessioniPrimary (citable) accession number: P29458
Secondary accession number(s): Q9P7K4, Q9USM0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: May 30, 2000
Last modified: May 23, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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