Reviewed, UniProtKB/Swiss-Prot P29456 (IL10_RAT)

Names and origin

Protein names

Recommended name:
    Interleukin-10
      Short name=IL-10
Alternative name(s):
    Cytokine synthesis inhibitory factor
      Short name=CSIF

Gene names

Name:	Il10
Synonyms:	Il-10

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the IL-10 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	immune response Ref.2 Inferred from mutant phenotype. Source: RGD inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of B cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of apoptosis Inferred from mutant phenotype. Source: RGD negative regulation of cytokine secretion during immune response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of inflammatory response Inferred from direct assay. Source: RGD negative regulation of interleukin-6 production Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of membrane protein ectodomain proteolysis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of nitric oxide biosynthetic process Inferred from direct assay. Source: RGD negative regulation of tumor necrosis factor biosynthetic process Inferred from direct assay. Source: RGD positive regulation of B cell apoptosis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cytokine secretion Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of gene-specific transcription Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB receptor biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB regulation of sensory perception of pain Inferred from direct assay. Source: RGD response to activity Inferred from expression pattern. Source: RGD response to drug Inferred from expression pattern. Source: RGD response to glucocorticoid stimulus Inferred from sequence or structural similarity. Source: UniProtKB response to insulin stimulus Inferred from expression pattern. Source: RGD response to lipopolysaccharide Inferred from expression pattern. Source: RGD
Cellular component	extracellular space Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW interleukin-10 receptor binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 178	160	Interleukin-10		PRO_0000015372
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Disulfide bond	30 ↔ 126		By similarity
	Disulfide bond	80 ↔ 132		By similarity
Experimental info
	Sequence conflict	2	1	P → L in CAA43090. Ref.2
	Sequence conflict	65	1	L → V in CAA43090. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P29456-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 0225AA6C63DAB70B	FASTA	178	20,426
	10 20 30 40 50 60 MPGSALLCCL LLLAGVKTSK GHSIRGDNNC THFPVSQTHM LRELRAAFSQ VKTFFQKKDQ 70 80 90 100 110 120 LDNILLTDSL LQDFKGYLGC QALSEMIKFY LVEVMPQAEN HGPEIKEHLN SLGEKLKTLW 130 140 150 160 170 IQLRRCHRFL PCENKSKAVE QVKNDFNKLQ DKGVYKAMNE FDIFINCIEA YVTLKMKN

References

[1]	"Synthesis and characterization of rat interleukin-10 (IL-10) cDNA clones from the RNA of cultured OX8-OX22-thoracic duct T cells." Goodman R.E., Oblak J., Bell R.G. Biochem. Biophys. Res. Commun. 189:1-7(1992) [PubMed: 1280414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages." Feng L., Tang W.W., Chang J.C.C., Wilson C.B. Biochem. Biophys. Res. Commun. 192:452-458(1993) [PubMed: 8484757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02926 mRNA. Translation: AAA41425.1. X60675 mRNA. Translation: CAA43090.1.
IPI	IPI00326111.
PIR	JN0475.
RefSeq	NP_036986.2.
UniGene	Rn.9868
3D structure databases
SMR	P29456. Positions 25-178.
ModBase	Search...
Protein-protein interaction databases
STRING	P29456.
Genome annotation databases
Ensembl	ENSRNOT00000006246; ENSRNOP00000006246; ENSRNOG00000004647; Rattus norvegicus. [Genome view]
GeneID	25325.
KEGG	rno:25325.
UCSC	NM_012854. rat.
Organism-specific databases
CTD	25325.
RGD	2886. Il10.
Phylogenomic databases
eggNOG	roNOG16135.
HOVERGEN	P29456.
InParanoid	P29456.
OMA	KGYLGCQ.
OrthoDB	EOG9M683W.
PhylomeDB	P29456.
Gene expression databases
ArrayExpress	P29456.
Genevestigator	P29456.
GermOnline	ENSRNOG00000004647. Rattus norvegicus.
Family and domain databases
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012352. IL-10_add_helix. IPR020443. Interleukin-10/19/20/24. IPR020423. Interleukin-10_CS. IPR000098. Interleukin_10. [Graphical view]
Gene3D	G3DSA:4.10.340.10. IL-10_add_helix. 1 hit.
PANTHER	PTHR11585. Interleukin_10. 1 hit.
Pfam	PF00726. IL10. 1 hit. [Graphical view]
PRINTS	PR01294. INTRLEUKIN10.
SMART	SM00188. IL10. 1 hit. [Graphical view]
PROSITE	PS00520. INTERLEUKIN_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606181.

Entry information

Entry name

IL10_RAT

Accession

Primary (citable) accession number: P29456
Secondary accession number(s): Q63263

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 19, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)