P29456 (IL10_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-10
Short name=IL-10

Alternative name(s):
Cytokine synthesis inhibitory factor
Short name=CSIF

Gene names

Name:	Il10
Synonyms:	Il-10

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the IL-10 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	branching involved in labyrinthine layer morphogenesis Inferred from electronic annotation. Source: Compara cellular response to estradiol stimulus Inferred from expression pattern PubMed 20735828. Source: RGD defense response to bacterium Inferred from electronic annotation. Source: Compara immune response Inferred from mutant phenotype Ref.2. Source: RGD inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of B cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of apoptotic process Inferred from mutant phenotype PubMed 19395591. Source: RGD negative regulation of chronic inflammatory response to antigenic stimulus Inferred from electronic annotation. Source: Compara negative regulation of cytokine secretion involved in immune response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of growth of symbiont in host Inferred from electronic annotation. Source: Compara negative regulation of inflammatory response Inferred from direct assay PubMed 17017555. Source: RGD negative regulation of interferon-gamma production Inferred from electronic annotation. Source: Compara negative regulation of interleukin-12 production Inferred from electronic annotation. Source: Compara negative regulation of interleukin-6 production Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of membrane protein ectodomain proteolysis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of myeloid dendritic cell activation Inferred from electronic annotation. Source: Compara negative regulation of nitric oxide biosynthetic process Inferred from direct assay PubMed 17017555. Source: RGD negative regulation of tumor necrosis factor biosynthetic process Inferred from direct assay PubMed 17017555. Source: RGD negative regulation of tumor necrosis factor production Inferred from electronic annotation. Source: Compara positive regulation of B cell apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of MHC class II biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of cytokine secretion Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB receptor biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB regulation of sensory perception of pain Inferred from direct assay PubMed 16949747. Source: RGD response to activity Inferred from expression pattern PubMed 19424714. Source: RGD response to carbon monoxide Inferred from expression pattern PubMed 20622590. Source: RGD response to drug Inferred from expression pattern PubMed 19419978PubMed 19422380. Source: RGD response to glucocorticoid stimulus Inferred from sequence or structural similarity. Source: UniProtKB response to inactivity Inferred from expression pattern PubMed 17033197. Source: RGD response to insulin stimulus Inferred from expression pattern PubMed 18791499. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 18791499PubMed 19390478. Source: RGD
Cellular_component	extracellular space Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	interleukin-10 receptor binding Inferred from direct assay PubMed 12405978. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 178	160	Interleukin-10		PRO_0000015372
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Disulfide bond	30 ↔ 126		By similarity
	Disulfide bond	80 ↔ 132		By similarity
Experimental info
	Sequence conflict	2	1	P → L in CAA43090. Ref.2
	Sequence conflict	65	1	L → V in CAA43090. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P29456 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 0225AA6C63DAB70B	FASTA	178	20,426
	10 20 30 40 50 60 MPGSALLCCL LLLAGVKTSK GHSIRGDNNC THFPVSQTHM LRELRAAFSQ VKTFFQKKDQ 70 80 90 100 110 120 LDNILLTDSL LQDFKGYLGC QALSEMIKFY LVEVMPQAEN HGPEIKEHLN SLGEKLKTLW 130 140 150 160 170 IQLRRCHRFL PCENKSKAVE QVKNDFNKLQ DKGVYKAMNE FDIFINCIEA YVTLKMKN

References

[1]	"Synthesis and characterization of rat interleukin-10 (IL-10) cDNA clones from the RNA of cultured OX8-OX22-thoracic duct T cells." Goodman R.E., Oblak J., Bell R.G. Biochem. Biophys. Res. Commun. 189:1-7(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages." Feng L., Tang W.W., Chang J.C.C., Wilson C.B. Biochem. Biophys. Res. Commun. 192:452-458(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02926 mRNA. Translation: AAA41425.1. X60675 mRNA. Translation: CAA43090.1.
IPI	IPI00326111.
PIR	JN0475.
RefSeq	NP_036986.2. NM_012854.2.
UniGene	Rn.9868.
3D structure databases
ProteinModelPortal	P29456.
SMR	P29456. Positions 25-178.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000006246.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000006246; ENSRNOP00000006246; ENSRNOG00000004647.
GeneID	25325.
KEGG	rno:25325.
UCSC	RGD:2886. rat.
Organism-specific databases
CTD	3586.
RGD	2886. Il10.
Phylogenomic databases
eggNOG	NOG43085.
GeneTree	ENSGT00510000048535.
HOGENOM	HOG000072692.
HOVERGEN	HBG004110.
InParanoid	P29456.
KO	K05443.
OMA	KGYLGCQ.
OrthoDB	EOG4F1X4G.
Gene expression databases
Genevestigator	P29456.
GermOnline	ENSRNOG00000004647. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR020443. Interleukin-10/19/20/24. IPR020423. Interleukin-10_CS. IPR000098. Interleukin_10. [Graphical view]
PANTHER	PTHR11585. PTHR11585. 1 hit.
Pfam	PF00726. IL10. 1 hit. [Graphical view]
PRINTS	PR01294. INTRLEUKIN10.
SMART	SM00188. IL10. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00520. INTERLEUKIN_10. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606181.

Entry information

Entry name

IL10_RAT

Accession

Primary (citable) accession number: P29456
Secondary accession number(s): Q63263

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program