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Protein

60S ribosomal protein L8-B

Gene

RPL8B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 80200 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eL8 in yeast.Curated

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of LSU-rRNA Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32145-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L8-B1 Publication
Alternative name(s):
L4
L4-1
Large ribosomal subunit protein eL8-B1 Publication
RP6
YL5
Gene namesi
Name:RPL8B1 Publication
Synonyms:KRB1, RPL4B
Ordered Locus Names:YLL045C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL045C.
SGDiS000003968. RPL8B.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001367612 – 25660S ribosomal protein L8-BAdd BLAST255

Proteomic databases

MaxQBiP29453.
PRIDEiP29453.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi31269. 336 interactors.
IntActiP29453. 107 interactors.
MINTiMINT-8285320.

Structurei

3D structure databases

ProteinModelPortaliP29453.
SMRiP29453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000004753.
HOGENOMiHOG000216644.
InParanoidiP29453.
KOiK02936.
OMAiYEEHRRQ.
OrthoDBiEOG092C4PDY.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiView protein in InterPro
IPR029064. L30e-like.
IPR001921. Ribosomal_L7A/L8.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
PfamiView protein in Pfam
PF01248. Ribosomal_L7Ae. 1 hit.
PRINTSiPR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiView protein in PROSITE
PS01082. RIBOSOMAL_L7AE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGKKVAPA PFGAKSTKSN KAKNPLTHST PKNFGIGQAV QPKRNLSRYV
60 70 80 90 100
KWPEYVRLQR QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE
110 120 130 140 150
TAAEKKERLT KEAAAIAEGK SKQDASPKPY AVKYGLNHVV SLIENKKAKL
160 170 180 190 200
VLIANDVDPI ELVVFLPALC KKMGVPYAII KGKARLGTLV NQKTSAVAAL
210 220 230 240 250
TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN KAQAKMDKRA

KTSDSA
Length:256
Mass (Da):28,112
Last modified:January 23, 2007 - v3
Checksum:iEAEF2DD981FD9C6E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114 – 115AA → G in AAA20990 (PubMed:8139928).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56835 Genomic DNA. Translation: CAA40165.1.
M88608 Genomic DNA. Translation: AAA20990.1.
Z73150 Genomic DNA. Translation: CAA97495.1.
BK006945 Genomic DNA. Translation: DAA09278.1.
PIRiS16810.
RefSeqiNP_013055.1. NM_001181865.1.

Genome annotation databases

EnsemblFungiiYLL045C; YLL045C; YLL045C.
GeneIDi850682.
KEGGisce:YLL045C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56835 Genomic DNA. Translation: CAA40165.1.
M88608 Genomic DNA. Translation: AAA20990.1.
Z73150 Genomic DNA. Translation: CAA97495.1.
BK006945 Genomic DNA. Translation: DAA09278.1.
PIRiS16810.
RefSeqiNP_013055.1. NM_001181865.1.

3D structure databases

ProteinModelPortaliP29453.
SMRiP29453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31269. 336 interactors.
IntActiP29453. 107 interactors.
MINTiMINT-8285320.

Proteomic databases

MaxQBiP29453.
PRIDEiP29453.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL045C; YLL045C; YLL045C.
GeneIDi850682.
KEGGisce:YLL045C.

Organism-specific databases

EuPathDBiFungiDB:YLL045C.
SGDiS000003968. RPL8B.

Phylogenomic databases

GeneTreeiENSGT00390000004753.
HOGENOMiHOG000216644.
InParanoidiP29453.
KOiK02936.
OMAiYEEHRRQ.
OrthoDBiEOG092C4PDY.

Enzyme and pathway databases

BioCyciYEAST:G3O-32145-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiPR:P29453.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiView protein in InterPro
IPR029064. L30e-like.
IPR001921. Ribosomal_L7A/L8.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
PfamiView protein in Pfam
PF01248. Ribosomal_L7Ae. 1 hit.
PRINTSiPR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiView protein in PROSITE
PS01082. RIBOSOMAL_L7AE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRL8B_YEAST
AccessioniPrimary (citable) accession number: P29453
Secondary accession number(s): D6VXW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: May 10, 2017
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.