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P29453

- RL8B_YEAST

UniProt

P29453 - RL8B_YEAST

Protein

60S ribosomal protein L8-B

Gene

RPL8B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. maturation of LSU-rRNA Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32145-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L8-B
    Alternative name(s):
    L4
    L4-1
    RP6
    YL5
    Gene namesi
    Name:RPL8B
    Synonyms:KRB1, RPL4B
    Ordered Locus Names:YLL045C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    SGDiS000003968. RPL8B.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 25625560S ribosomal protein L8-BPRO_0000136761Add
    BLAST

    Proteomic databases

    MaxQBiP29453.
    PaxDbiP29453.
    PeptideAtlasiP29453.
    PRIDEiP29453.

    Expressioni

    Gene expression databases

    GenevestigatoriP29453.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi31269. 189 interactions.
    IntActiP29453. 105 interactions.
    MINTiMINT-8285320.
    STRINGi4932.YLL045C.

    Structurei

    3D structure databases

    ProteinModelPortaliP29453.
    SMRiP29453. Positions 24-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L7Ae family.Curated

    Phylogenomic databases

    eggNOGiCOG1358.
    GeneTreeiENSGT00390000004753.
    HOGENOMiHOG000216644.
    KOiK02936.
    OMAiVENKKAN.
    OrthoDBiEOG75QRG2.

    Family and domain databases

    Gene3Di3.30.1330.30. 1 hit.
    InterProiIPR029064. L30e-like.
    IPR001921. Ribosomal_L7A/L8.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    IPR018492. Ribosomal_L7Ae/L8/Nhp2.
    IPR004037. Ribosomal_L7Ae_CS.
    [Graphical view]
    PANTHERiPTHR23105:SF1. PTHR23105:SF1. 1 hit.
    PfamiPF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view]
    PRINTSiPR00881. L7ARS6FAMILY.
    PR00882. RIBOSOMALL7A.
    SUPFAMiSSF55315. SSF55315. 1 hit.
    PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29453-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPGKKVAPA PFGAKSTKSN KAKNPLTHST PKNFGIGQAV QPKRNLSRYV    50
    KWPEYVRLQR QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE 100
    TAAEKKERLT KEAAAIAEGK SKQDASPKPY AVKYGLNHVV SLIENKKAKL 150
    VLIANDVDPI ELVVFLPALC KKMGVPYAII KGKARLGTLV NQKTSAVAAL 200
    TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN KAQAKMDKRA 250
    KTSDSA 256
    Length:256
    Mass (Da):28,112
    Last modified:January 23, 2007 - v3
    Checksum:iEAEF2DD981FD9C6E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti114 – 1152AA → G in AAA20990. (PubMed:8139928)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56835 Genomic DNA. Translation: CAA40165.1.
    M88608 Genomic DNA. Translation: AAA20990.1.
    Z73150 Genomic DNA. Translation: CAA97495.1.
    BK006945 Genomic DNA. Translation: DAA09278.1.
    PIRiS16810.
    RefSeqiNP_013055.1. NM_001181865.1.

    Genome annotation databases

    EnsemblFungiiYLL045C; YLL045C; YLL045C.
    GeneIDi850682.
    KEGGisce:YLL045C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56835 Genomic DNA. Translation: CAA40165.1 .
    M88608 Genomic DNA. Translation: AAA20990.1 .
    Z73150 Genomic DNA. Translation: CAA97495.1 .
    BK006945 Genomic DNA. Translation: DAA09278.1 .
    PIRi S16810.
    RefSeqi NP_013055.1. NM_001181865.1.

    3D structure databases

    ProteinModelPortali P29453.
    SMRi P29453. Positions 24-256.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31269. 189 interactions.
    IntActi P29453. 105 interactions.
    MINTi MINT-8285320.
    STRINGi 4932.YLL045C.

    Proteomic databases

    MaxQBi P29453.
    PaxDbi P29453.
    PeptideAtlasi P29453.
    PRIDEi P29453.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLL045C ; YLL045C ; YLL045C .
    GeneIDi 850682.
    KEGGi sce:YLL045C.

    Organism-specific databases

    SGDi S000003968. RPL8B.

    Phylogenomic databases

    eggNOGi COG1358.
    GeneTreei ENSGT00390000004753.
    HOGENOMi HOG000216644.
    KOi K02936.
    OMAi VENKKAN.
    OrthoDBi EOG75QRG2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32145-MONOMER.

    Miscellaneous databases

    NextBioi 966683.

    Gene expression databases

    Genevestigatori P29453.

    Family and domain databases

    Gene3Di 3.30.1330.30. 1 hit.
    InterProi IPR029064. L30e-like.
    IPR001921. Ribosomal_L7A/L8.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    IPR018492. Ribosomal_L7Ae/L8/Nhp2.
    IPR004037. Ribosomal_L7Ae_CS.
    [Graphical view ]
    PANTHERi PTHR23105:SF1. PTHR23105:SF1. 1 hit.
    Pfami PF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view ]
    PRINTSi PR00881. L7ARS6FAMILY.
    PR00882. RIBOSOMALL7A.
    SUPFAMi SSF55315. SSF55315. 1 hit.
    PROSITEi PS01082. RIBOSOMAL_L7AE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a."
      Yon J., Giallongo A., Fried M.
      Mol. Gen. Genet. 227:72-80(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "RNP1, a new ribonucleoprotein gene of the yeast Saccharomyces cerevisiae."
      Cusick M.E.
      Nucleic Acids Res. 22:869-877(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Purification and identification of two major single-stranded binding proteins of yeast Saccharomyces cerevisiae as ribosomal protein L4 and histone H2B."
      Cusick M.E.
      Biochim. Biophys. Acta 1217:31-40(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
      Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
      J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-20.
    7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRL8B_YEAST
    AccessioniPrimary (citable) accession number: P29453
    Secondary accession number(s): D6VXW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 80200 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for L8 in yeast.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Ribosomal proteins
      Ribosomal proteins families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3