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P29453 (RL8B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
60S ribosomal protein L8-B
Alternative name(s):
L4
L4-1
RP6
YL5
Gene names
Name:RPL8B
Synonyms:KRB1, RPL4B
Ordered Locus Names:YLL045C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7

Subcellular location

Cytoplasm Ref.8.

Miscellaneous

Present with 80200 molecules/cell in log phase SD medium.

There are 2 genes for L8 in yeast.

Sequence similarities

Belongs to the ribosomal protein L7Ae family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 25625560S ribosomal protein L8-B
PRO_0000136761

Experimental info

Sequence conflict114 – 1152AA → G in AAA20990. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P29453 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: EAEF2DD981FD9C6E

FASTA25628,112
        10         20         30         40         50         60 
MAPGKKVAPA PFGAKSTKSN KAKNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRLQR 

        70         80         90        100        110        120 
QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE TAAEKKERLT KEAAAIAEGK 

       130        140        150        160        170        180 
SKQDASPKPY AVKYGLNHVV SLIENKKAKL VLIANDVDPI ELVVFLPALC KKMGVPYAII 

       190        200        210        220        230        240 
KGKARLGTLV NQKTSAVAAL TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN 

       250 
KAQAKMDKRA KTSDSA 

« Hide

References

« Hide 'large scale' references
[1]"The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a."
Yon J., Giallongo A., Fried M.
Mol. Gen. Genet. 227:72-80(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"RNP1, a new ribonucleoprotein gene of the yeast Saccharomyces cerevisiae."
Cusick M.E.
Nucleic Acids Res. 22:869-877(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Purification and identification of two major single-stranded binding proteins of yeast Saccharomyces cerevisiae as ribosomal protein L4 and histone H2B."
Cusick M.E.
Biochim. Biophys. Acta 1217:31-40(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-20.
[7]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56835 Genomic DNA. Translation: CAA40165.1.
M88608 Genomic DNA. Translation: AAA20990.1.
Z73150 Genomic DNA. Translation: CAA97495.1.
BK006945 Genomic DNA. Translation: DAA09278.1.
PIRS16810.
RefSeqNP_013055.1. NM_001181865.1.

3D structure databases

ProteinModelPortalP29453.
SMRP29453. Positions 24-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31269. 188 interactions.
IntActP29453. 105 interactions.
MINTMINT-8285320.
STRING4932.YLL045C.

Proteomic databases

MaxQBP29453.
PaxDbP29453.
PeptideAtlasP29453.
PRIDEP29453.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLL045C; YLL045C; YLL045C.
GeneID850682.
KEGGsce:YLL045C.

Organism-specific databases

SGDS000003968. RPL8B.

Phylogenomic databases

eggNOGCOG1358.
GeneTreeENSGT00390000004753.
HOGENOMHOG000216644.
KOK02936.
OMAVENKKAN.
OrthoDBEOG75QRG2.

Enzyme and pathway databases

BioCycYEAST:G3O-32145-MONOMER.

Gene expression databases

GenevestigatorP29453.

Family and domain databases

Gene3D3.30.1330.30. 1 hit.
InterProIPR029064. L30e-like.
IPR001921. Ribosomal_L7A/L8.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view]
PANTHERPTHR23105:SF1. PTHR23105:SF1. 1 hit.
PfamPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSPR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.
SUPFAMSSF55315. SSF55315. 1 hit.
PROSITEPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966683.

Entry information

Entry nameRL8B_YEAST
AccessionPrimary (citable) accession number: P29453
Secondary accession number(s): D6VXW2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries