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P29453

- RL8B_YEAST

UniProt

P29453 - RL8B_YEAST

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Protein

60S ribosomal protein L8-B

Gene

RPL8B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. maturation of LSU-rRNA Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32145-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L8-B
Alternative name(s):
L4
L4-1
RP6
YL5
Gene namesi
Name:RPL8B
Synonyms:KRB1, RPL4B
Ordered Locus Names:YLL045C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

SGDiS000003968. RPL8B.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 25625560S ribosomal protein L8-BPRO_0000136761Add
BLAST

Proteomic databases

MaxQBiP29453.
PaxDbiP29453.
PeptideAtlasiP29453.
PRIDEiP29453.

Expressioni

Gene expression databases

GenevestigatoriP29453.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi31269. 191 interactions.
IntActiP29453. 105 interactions.
MINTiMINT-8285320.
STRINGi4932.YLL045C.

Structurei

3D structure databases

ProteinModelPortaliP29453.
SMRiP29453. Positions 24-251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L7Ae family.Curated

Phylogenomic databases

eggNOGiCOG1358.
GeneTreeiENSGT00390000004753.
HOGENOMiHOG000216644.
InParanoidiP29453.
KOiK02936.
OMAiVENKKAN.
OrthoDBiEOG75QRG2.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR029064. L30e-like.
IPR001921. Ribosomal_L7A/L8.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view]
PANTHERiPTHR23105:SF1. PTHR23105:SF1. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29453-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPGKKVAPA PFGAKSTKSN KAKNPLTHST PKNFGIGQAV QPKRNLSRYV
60 70 80 90 100
KWPEYVRLQR QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE
110 120 130 140 150
TAAEKKERLT KEAAAIAEGK SKQDASPKPY AVKYGLNHVV SLIENKKAKL
160 170 180 190 200
VLIANDVDPI ELVVFLPALC KKMGVPYAII KGKARLGTLV NQKTSAVAAL
210 220 230 240 250
TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN KAQAKMDKRA

KTSDSA
Length:256
Mass (Da):28,112
Last modified:January 23, 2007 - v3
Checksum:iEAEF2DD981FD9C6E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1152AA → G in AAA20990. (PubMed:8139928)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56835 Genomic DNA. Translation: CAA40165.1.
M88608 Genomic DNA. Translation: AAA20990.1.
Z73150 Genomic DNA. Translation: CAA97495.1.
BK006945 Genomic DNA. Translation: DAA09278.1.
PIRiS16810.
RefSeqiNP_013055.1. NM_001181865.1.

Genome annotation databases

EnsemblFungiiYLL045C; YLL045C; YLL045C.
GeneIDi850682.
KEGGisce:YLL045C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56835 Genomic DNA. Translation: CAA40165.1 .
M88608 Genomic DNA. Translation: AAA20990.1 .
Z73150 Genomic DNA. Translation: CAA97495.1 .
BK006945 Genomic DNA. Translation: DAA09278.1 .
PIRi S16810.
RefSeqi NP_013055.1. NM_001181865.1.

3D structure databases

ProteinModelPortali P29453.
SMRi P29453. Positions 24-251.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31269. 191 interactions.
IntActi P29453. 105 interactions.
MINTi MINT-8285320.
STRINGi 4932.YLL045C.

Proteomic databases

MaxQBi P29453.
PaxDbi P29453.
PeptideAtlasi P29453.
PRIDEi P29453.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLL045C ; YLL045C ; YLL045C .
GeneIDi 850682.
KEGGi sce:YLL045C.

Organism-specific databases

SGDi S000003968. RPL8B.

Phylogenomic databases

eggNOGi COG1358.
GeneTreei ENSGT00390000004753.
HOGENOMi HOG000216644.
InParanoidi P29453.
KOi K02936.
OMAi VENKKAN.
OrthoDBi EOG75QRG2.

Enzyme and pathway databases

BioCyci YEAST:G3O-32145-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

NextBioi 966683.

Gene expression databases

Genevestigatori P29453.

Family and domain databases

Gene3Di 3.30.1330.30. 1 hit.
InterProi IPR029064. L30e-like.
IPR001921. Ribosomal_L7A/L8.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view ]
PANTHERi PTHR23105:SF1. PTHR23105:SF1. 1 hit.
Pfami PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view ]
PRINTSi PR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.
SUPFAMi SSF55315. SSF55315. 1 hit.
PROSITEi PS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a."
    Yon J., Giallongo A., Fried M.
    Mol. Gen. Genet. 227:72-80(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "RNP1, a new ribonucleoprotein gene of the yeast Saccharomyces cerevisiae."
    Cusick M.E.
    Nucleic Acids Res. 22:869-877(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Purification and identification of two major single-stranded binding proteins of yeast Saccharomyces cerevisiae as ribosomal protein L4 and histone H2B."
    Cusick M.E.
    Biochim. Biophys. Acta 1217:31-40(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
    Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
    J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20.
  7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRL8B_YEAST
AccessioniPrimary (citable) accession number: P29453
Secondary accession number(s): D6VXW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 80200 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L8 in yeast.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3