P29453 (RL8B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L8-B

Alternative name(s):
L4
L4-1
RP6
YL5

Gene names

Name:	RPL8B
Synonyms:	KRB1, RPL4B
Ordered Locus Names:	YLL045C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7
Subcellular location	Cytoplasm Ref.8.
Miscellaneous	Present with 80200 molecules/cell in log phase SD medium. There are 2 genes for L8 in yeast.
Sequence similarities	Belongs to the ribosomal protein L7Ae family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.7. Source: SGD ribosome biogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.7. Source: SGD
Molecular_function	structural constituent of ribosome Traceable author statement Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 256	255	60S ribosomal protein L8-B		PRO_0000136761
Experimental info
	Sequence conflict	114 – 115	2	AA → G in AAA20990. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P29453 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: EAEF2DD981FD9C6E	FASTA	256	28,112
	10 20 30 40 50 60 MAPGKKVAPA PFGAKSTKSN KAKNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRLQR 70 80 90 100 110 120 QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE TAAEKKERLT KEAAAIAEGK 130 140 150 160 170 180 SKQDASPKPY AVKYGLNHVV SLIENKKAKL VLIANDVDPI ELVVFLPALC KKMGVPYAII 190 200 210 220 230 240 KGKARLGTLV NQKTSAVAAL TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN 250 KAQAKMDKRA KTSDSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a." Yon J., Giallongo A., Fried M. Mol. Gen. Genet. 227:72-80(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"RNP1, a new ribonucleoprotein gene of the yeast Saccharomyces cerevisiae." Cusick M.E. Nucleic Acids Res. 22:869-877(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Purification and identification of two major single-stranded binding proteins of yeast Saccharomyces cerevisiae as ribosomal protein L4 and histone H2B." Cusick M.E. Biochim. Biophys. Acta 1217:31-40(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56835 Genomic DNA. Translation: CAA40165.1. M88608 Genomic DNA. Translation: AAA20990.1. Z73150 Genomic DNA. Translation: CAA97495.1. BK006945 Genomic DNA. Translation: DAA09278.1.
PIR	S16810.
RefSeq	NP_013055.1. NM_001181865.1.
3D structure databases
ProteinModelPortal	P29453.
SMR	P29453. Positions 24-256.
ModBase	Search...
Protein-protein interaction databases
IntAct	P29453. 101 interactions.
MINT	MINT-8285320.
STRING	4932.YLL045C.
Proteomic databases
PaxDb	P29453.
PeptideAtlas	P29453.
PRIDE	P29453.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLL045C; YLL045C; YLL045C.
GeneID	850682.
KEGG	sce:YLL045C.
Organism-specific databases
SGD	S000003968. RPL8B.
Phylogenomic databases
eggNOG	COG1358.
GeneTree	ENSGT00390000004753.
HOGENOM	HOG000216644.
KO	K02936.
OMA	QRSILYK.
OrthoDB	EOG4KWP2Z.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32145-MONOMER.
Gene expression databases
Genevestigator	P29453.
GermOnline	YLL045C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001921. Ribosomal_L7A/L8. IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45. IPR018492. Ribosomal_L7Ae/L8/Nhp2. IPR004037. Ribosomal_L7Ae_CS. [Graphical view]
PANTHER	PTHR23105:SF1. PTHR23105:SF1. 1 hit.
Pfam	PF01248. Ribosomal_L7Ae. 1 hit. [Graphical view]
PRINTS	PR00881. L7ARS6FAMILY. PR00882. RIBOSOMALL7A.
PROSITE	PS01082. RIBOSOMAL_L7AE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966683.

Entry information

Entry name

RL8B_YEAST

Accession

Primary (citable) accession number: P29453
Secondary accession number(s): D6VXW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program