Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P29452

- CASP1_MOUSE

UniProt

P29452 - CASP1_MOUSE

Protein

Caspase-1

Gene

Casp1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

    Catalytic activityi

    Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei236 – 2361By similarity
    Active sitei284 – 2841By similarity

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: RefGenome
    2. peptidase activity Source: MGI
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cellular response to mechanical stimulus Source: Ensembl
    2. cellular response to organic substance Source: MGI
    3. execution phase of apoptosis Source: RefGenome
    4. interleukin-1 beta production Source: MGI
    5. membrane hyperpolarization Source: MGI
    6. mitochondrial depolarization Source: MGI
    7. positive regulation of cytokine secretion Source: MGI
    8. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    9. positive regulation of interleukin-1 alpha secretion Source: MGI
    10. positive regulation of interleukin-1 beta secretion Source: MGI
    11. programmed necrotic cell death Source: MGI
    12. protein processing Source: MGI
    13. pyroptosis Source: MGI
    14. regulation of apoptotic process Source: InterPro
    15. regulation of inflammatory response Source: MGI
    16. response to ATP Source: MGI
    17. response to hypoxia Source: MGI
    18. response to lipopolysaccharide Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Apoptosis

    Enzyme and pathway databases

    BRENDAi3.4.22.36. 3474.
    ReactomeiREACT_198662. The AIM2 inflammasome.
    REACT_198700. Interleukin-1 processing.

    Protein family/group databases

    MEROPSiC14.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Caspase-1 (EC:3.4.22.36)
    Short name:
    CASP-1
    Alternative name(s):
    Interleukin-1 beta convertase
    Short name:
    IL-1BC
    Interleukin-1 beta-converting enzyme
    Short name:
    ICE
    Short name:
    IL-1 beta-converting enzyme
    p45
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Casp1
    Synonyms:Il1bc
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:96544. Casp1.

    Subcellular locationi

    GO - Cellular componenti

    1. AIM2 inflammasome complex Source: UniProtKB
    2. cytoplasm Source: MGI
    3. cytosol Source: Reactome
    4. extracellular region Source: MGI
    5. IPAF inflammasome complex Source: UniProtKB
    6. NLRP1 inflammasome complex Source: UniProtKB
    7. NLRP3 inflammasome complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – ?118118Sequence AnalysisPRO_0000004525Add
    BLAST
    Chaini?119 – 296178Caspase-1 subunit p20PRO_0000004526Add
    BLAST
    Propeptidei297 – 31418Sequence AnalysisPRO_0000004527Add
    BLAST
    Chaini315 – 40288Caspase-1 subunit p10PRO_0000004528Add
    BLAST

    Post-translational modificationi

    The two subunits are derived from the precursor sequence by a autocatalytic mechanism.

    Keywords - PTMi

    Zymogen

    Proteomic databases

    MaxQBiP29452.
    PaxDbiP29452.
    PRIDEiP29452.

    PTM databases

    PhosphoSiteiP29452.

    Expressioni

    Tissue specificityi

    High level expression seen in spleen and lung, low level expression seen in brain, heart, liver, kidney, testis and skeletal muscle.

    Gene expression databases

    BgeeiP29452.
    CleanExiMM_CASP1.
    GenevestigatoriP29452.

    Interactioni

    Subunit structurei

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p20 and p10 subunits interact with MEFV By similarity. Interacts with CARD17/INCA and CARD18 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ipaBP180113EBI-489700,EBI-490239From a different organism.
    sipBQ561342EBI-489700,EBI-489689From a different organism.

    Protein-protein interaction databases

    DIPiDIP-34659N.
    IntActiP29452. 2 interactions.
    STRINGi10090.ENSMUSP00000027015.

    Structurei

    3D structure databases

    ProteinModelPortaliP29452.
    SMRiP29452. Positions 2-90, 127-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 9191CARDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C14A family.Curated
    Contains 1 CARD domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG326166.
    GeneTreeiENSGT00700000104134.
    HOGENOMiHOG000234399.
    HOVERGENiHBG076981.
    InParanoidiP29452.
    KOiK01370.
    OMAiKSAEIYP.
    OrthoDBiEOG7FXZZ6.
    PhylomeDBiP29452.
    TreeFamiTF102023.

    Family and domain databases

    Gene3Di1.10.533.10. 1 hit.
    3.40.50.1460. 1 hit.
    InterProiIPR001315. CARD.
    IPR029030. Caspase-like_dom.
    IPR017350. Caspase_ICE-type.
    IPR011029. DEATH-like_dom.
    IPR011600. Pept_C14_caspase.
    IPR001309. Pept_C14_ICE_p20.
    IPR016129. Pept_C14_ICE_p20_AS.
    IPR002138. Pept_C14_p10.
    IPR015917. Pept_C14A_p45_core.
    [Graphical view]
    PfamiPF00619. CARD. 1 hit.
    PF00656. Peptidase_C14. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
    PRINTSiPR00376. IL1BCENZYME.
    SMARTiSM00114. CARD. 1 hit.
    SM00115. CASc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47986. SSF47986. 1 hit.
    PROSITEiPS50209. CARD. 1 hit.
    PS01122. CASPASE_CYS. 1 hit.
    PS01121. CASPASE_HIS. 1 hit.
    PS50207. CASPASE_P10. 1 hit.
    PS50208. CASPASE_P20. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29452-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADKILRAKR KQFINSVSIG TINGLLDELL EKRVLNQEEM DKIKLANITA    50
    MDKARDLCDH VSKKGPQASQ IFITYICNED CYLAGILELQ SAPSAETFVA 100
    TEDSKGGHPS SSETKEEQNK EDGTFPGLTG TLKFCPLEKA QKLWKENPSE 150
    IYPIMNTTTR TRLALIICNT EFQHLSPRVG AQVDLREMKL LLEDLGYTVK 200
    VKENLTALEM VKEVKEFAAC PEHKTSDSTF LVFMSHGIQE GICGTTYSNE 250
    VSDILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKDSVRD 300
    SEEDFLTDAI FEDDGIKKAH IEKDFIAFCS STPDNVSWRH PVRGSLFIES 350
    LIKHMKEYAW SCDLEDIFRK VRFSFEQPEF RLQMPTADRV TLTKRFYLFP 400
    GH 402
    Length:402
    Mass (Da):45,640
    Last modified:April 1, 1993 - v1
    Checksum:i3BEBCEFA67C69B03
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 64DKIL → V in AAA56992. (PubMed:8034321)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03799 mRNA. Translation: AAA39306.1.
    L28095 mRNA. Translation: AAA20209.1.
    U04269 Genomic DNA. Translation: AAA56992.1.
    BC008152 mRNA. Translation: AAH08152.1.
    CCDSiCCDS22798.1.
    PIRiA46495.
    RefSeqiNP_033937.2. NM_009807.2.
    UniGeneiMm.1051.

    Genome annotation databases

    EnsembliENSMUST00000027015; ENSMUSP00000027015; ENSMUSG00000025888.
    GeneIDi12362.
    KEGGimmu:12362.
    UCSCiuc009obr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L03799 mRNA. Translation: AAA39306.1 .
    L28095 mRNA. Translation: AAA20209.1 .
    U04269 Genomic DNA. Translation: AAA56992.1 .
    BC008152 mRNA. Translation: AAH08152.1 .
    CCDSi CCDS22798.1.
    PIRi A46495.
    RefSeqi NP_033937.2. NM_009807.2.
    UniGenei Mm.1051.

    3D structure databases

    ProteinModelPortali P29452.
    SMRi P29452. Positions 2-90, 127-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-34659N.
    IntActi P29452. 2 interactions.
    STRINGi 10090.ENSMUSP00000027015.

    Chemistry

    BindingDBi P29452.
    ChEMBLi CHEMBL4800.

    Protein family/group databases

    MEROPSi C14.001.

    PTM databases

    PhosphoSitei P29452.

    Proteomic databases

    MaxQBi P29452.
    PaxDbi P29452.
    PRIDEi P29452.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027015 ; ENSMUSP00000027015 ; ENSMUSG00000025888 .
    GeneIDi 12362.
    KEGGi mmu:12362.
    UCSCi uc009obr.1. mouse.

    Organism-specific databases

    CTDi 834.
    MGIi MGI:96544. Casp1.

    Phylogenomic databases

    eggNOGi NOG326166.
    GeneTreei ENSGT00700000104134.
    HOGENOMi HOG000234399.
    HOVERGENi HBG076981.
    InParanoidi P29452.
    KOi K01370.
    OMAi KSAEIYP.
    OrthoDBi EOG7FXZZ6.
    PhylomeDBi P29452.
    TreeFami TF102023.

    Enzyme and pathway databases

    BRENDAi 3.4.22.36. 3474.
    Reactomei REACT_198662. The AIM2 inflammasome.
    REACT_198700. Interleukin-1 processing.

    Miscellaneous databases

    NextBioi 281032.
    PROi P29452.
    SOURCEi Search...

    Gene expression databases

    Bgeei P29452.
    CleanExi MM_CASP1.
    Genevestigatori P29452.

    Family and domain databases

    Gene3Di 1.10.533.10. 1 hit.
    3.40.50.1460. 1 hit.
    InterProi IPR001315. CARD.
    IPR029030. Caspase-like_dom.
    IPR017350. Caspase_ICE-type.
    IPR011029. DEATH-like_dom.
    IPR011600. Pept_C14_caspase.
    IPR001309. Pept_C14_ICE_p20.
    IPR016129. Pept_C14_ICE_p20_AS.
    IPR002138. Pept_C14_p10.
    IPR015917. Pept_C14A_p45_core.
    [Graphical view ]
    Pfami PF00619. CARD. 1 hit.
    PF00656. Peptidase_C14. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038001. Caspase_ICE. 1 hit.
    PRINTSi PR00376. IL1BCENZYME.
    SMARTi SM00114. CARD. 1 hit.
    SM00115. CASc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47986. SSF47986. 1 hit.
    PROSITEi PS50209. CARD. 1 hit.
    PS01122. CASPASE_CYS. 1 hit.
    PS01121. CASPASE_HIS. 1 hit.
    PS50207. CASPASE_P10. 1 hit.
    PS50208. CASPASE_P20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the murine IL-1 beta converting enzyme cDNA."
      Nett-Fiordalisi M.A., Cerretti D.P., Berson D.R., Gilbert D.J., Jenkins N.A., Copeland N.G., Black R.A., Chaplin D.D.
      J. Immunol. 149:3254-3259(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Interleukin 1 beta (IL-1 beta) processing in murine macrophages requires a structurally conserved homologue of human IL-1 beta converting enzyme."
      Molineaux S.M., Casano F.J., Rolando A.M., Peterson E.P., Limjuco G., Chin J., Griffin P.R., Calaycay J.R., Ding G.J.-F., Yamin T.-T., Palyha O.C., Luell S., Fletcher D., Miller D.K., Howard A.D., Thornberry N.A., Kostura M.J.
      Proc. Natl. Acad. Sci. U.S.A. 90:1809-1813(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The structure and complete nucleotide sequence of the murine gene encoding interleukin-1 beta converting enzyme (ICE)."
      Casano F.J., Rolando A.M., Mudgett J.S., Molineaux S.M.
      Genomics 20:474-481(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. Cited for: CHARACTERIZATION.
      Strain: C3H/An.

    Entry informationi

    Entry nameiCASP1_MOUSE
    AccessioniPrimary (citable) accession number: P29452
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 144 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3