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P29452

- CASP1_MOUSE

UniProt

P29452 - CASP1_MOUSE

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Protein

Caspase-1

Gene

Casp1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

Catalytic activityi

Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei236 – 2361By similarity
Active sitei284 – 2841By similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: RefGenome
  2. peptidase activity Source: MGI

GO - Biological processi

  1. cellular response to mechanical stimulus Source: Ensembl
  2. cellular response to organic substance Source: MGI
  3. execution phase of apoptosis Source: RefGenome
  4. interleukin-1 beta production Source: MGI
  5. membrane hyperpolarization Source: MGI
  6. mitochondrial depolarization Source: MGI
  7. positive regulation of cytokine secretion Source: MGI
  8. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  9. positive regulation of interleukin-1 alpha secretion Source: MGI
  10. positive regulation of interleukin-1 beta secretion Source: MGI
  11. programmed necrotic cell death Source: MGI
  12. protein processing Source: MGI
  13. pyroptosis Source: MGI
  14. regulation of apoptotic process Source: InterPro
  15. regulation of inflammatory response Source: MGI
  16. response to ATP Source: MGI
  17. response to hypoxia Source: MGI
  18. response to lipopolysaccharide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BRENDAi3.4.22.36. 3474.
ReactomeiREACT_198662. The AIM2 inflammasome.
REACT_198700. Interleukin-1 processing.

Protein family/group databases

MEROPSiC14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-1 (EC:3.4.22.36)
Short name:
CASP-1
Alternative name(s):
Interleukin-1 beta convertase
Short name:
IL-1BC
Interleukin-1 beta-converting enzyme
Short name:
ICE
Short name:
IL-1 beta-converting enzyme
p45
Cleaved into the following 2 chains:
Gene namesi
Name:Casp1
Synonyms:Il1bc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:96544. Casp1.

Subcellular locationi

GO - Cellular componenti

  1. AIM2 inflammasome complex Source: UniProtKB
  2. cytoplasm Source: MGI
  3. cytosol Source: Reactome
  4. extracellular region Source: MGI
  5. IPAF inflammasome complex Source: UniProtKB
  6. NLRP1 inflammasome complex Source: UniProtKB
  7. NLRP3 inflammasome complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – ?118118Sequence AnalysisPRO_0000004525Add
BLAST
Chaini?119 – 296178Caspase-1 subunit p20PRO_0000004526Add
BLAST
Propeptidei297 – 31418Sequence AnalysisPRO_0000004527Add
BLAST
Chaini315 – 40288Caspase-1 subunit p10PRO_0000004528Add
BLAST

Post-translational modificationi

The two subunits are derived from the precursor sequence by a autocatalytic mechanism.

Keywords - PTMi

Zymogen

Proteomic databases

MaxQBiP29452.
PaxDbiP29452.
PRIDEiP29452.

PTM databases

PhosphoSiteiP29452.

Expressioni

Tissue specificityi

High level expression seen in spleen and lung, low level expression seen in brain, heart, liver, kidney, testis and skeletal muscle.

Gene expression databases

BgeeiP29452.
CleanExiMM_CASP1.
GenevestigatoriP29452.

Interactioni

Subunit structurei

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p20 and p10 subunits interact with MEFV (By similarity). Interacts with CARD17/INCA and CARD18 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ipaBP180113EBI-489700,EBI-490239From a different organism.
sipBQ561342EBI-489700,EBI-489689From a different organism.

Protein-protein interaction databases

DIPiDIP-34659N.
IntActiP29452. 2 interactions.
STRINGi10090.ENSMUSP00000027015.

Structurei

3D structure databases

ProteinModelPortaliP29452.
SMRiP29452. Positions 2-90, 127-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9191CARDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated
Contains 1 CARD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG326166.
GeneTreeiENSGT00760000118912.
HOGENOMiHOG000234399.
HOVERGENiHBG076981.
InParanoidiP29452.
KOiK01370.
OMAiKSAEIYP.
OrthoDBiEOG7FXZZ6.
PhylomeDBiP29452.
TreeFamiTF102023.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProiIPR001315. CARD.
IPR029030. Caspase-like_dom.
IPR017350. Caspase_ICE-type.
IPR011029. DEATH-like_dom.
IPR011600. Pept_C14_caspase.
IPR001309. Pept_C14_ICE_p20.
IPR016129. Pept_C14_ICE_p20_AS.
IPR002138. Pept_C14_p10.
IPR015917. Pept_C14A_p45_core.
[Graphical view]
PfamiPF00619. CARD. 1 hit.
PF00656. Peptidase_C14. 1 hit.
[Graphical view]
PIRSFiPIRSF038001. Caspase_ICE. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiSM00114. CARD. 1 hit.
SM00115. CASc. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50209. CARD. 1 hit.
PS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29452-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADKILRAKR KQFINSVSIG TINGLLDELL EKRVLNQEEM DKIKLANITA
60 70 80 90 100
MDKARDLCDH VSKKGPQASQ IFITYICNED CYLAGILELQ SAPSAETFVA
110 120 130 140 150
TEDSKGGHPS SSETKEEQNK EDGTFPGLTG TLKFCPLEKA QKLWKENPSE
160 170 180 190 200
IYPIMNTTTR TRLALIICNT EFQHLSPRVG AQVDLREMKL LLEDLGYTVK
210 220 230 240 250
VKENLTALEM VKEVKEFAAC PEHKTSDSTF LVFMSHGIQE GICGTTYSNE
260 270 280 290 300
VSDILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKDSVRD
310 320 330 340 350
SEEDFLTDAI FEDDGIKKAH IEKDFIAFCS STPDNVSWRH PVRGSLFIES
360 370 380 390 400
LIKHMKEYAW SCDLEDIFRK VRFSFEQPEF RLQMPTADRV TLTKRFYLFP

GH
Length:402
Mass (Da):45,640
Last modified:April 1, 1993 - v1
Checksum:i3BEBCEFA67C69B03
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 64DKIL → V in AAA56992. (PubMed:8034321)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L03799 mRNA. Translation: AAA39306.1.
L28095 mRNA. Translation: AAA20209.1.
U04269 Genomic DNA. Translation: AAA56992.1.
BC008152 mRNA. Translation: AAH08152.1.
CCDSiCCDS22798.1.
PIRiA46495.
RefSeqiNP_033937.2. NM_009807.2.
UniGeneiMm.1051.

Genome annotation databases

EnsembliENSMUST00000027015; ENSMUSP00000027015; ENSMUSG00000025888.
GeneIDi12362.
KEGGimmu:12362.
UCSCiuc009obr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L03799 mRNA. Translation: AAA39306.1 .
L28095 mRNA. Translation: AAA20209.1 .
U04269 Genomic DNA. Translation: AAA56992.1 .
BC008152 mRNA. Translation: AAH08152.1 .
CCDSi CCDS22798.1.
PIRi A46495.
RefSeqi NP_033937.2. NM_009807.2.
UniGenei Mm.1051.

3D structure databases

ProteinModelPortali P29452.
SMRi P29452. Positions 2-90, 127-402.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-34659N.
IntActi P29452. 2 interactions.
STRINGi 10090.ENSMUSP00000027015.

Chemistry

BindingDBi P29452.
ChEMBLi CHEMBL4800.

Protein family/group databases

MEROPSi C14.001.

PTM databases

PhosphoSitei P29452.

Proteomic databases

MaxQBi P29452.
PaxDbi P29452.
PRIDEi P29452.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027015 ; ENSMUSP00000027015 ; ENSMUSG00000025888 .
GeneIDi 12362.
KEGGi mmu:12362.
UCSCi uc009obr.1. mouse.

Organism-specific databases

CTDi 834.
MGIi MGI:96544. Casp1.

Phylogenomic databases

eggNOGi NOG326166.
GeneTreei ENSGT00760000118912.
HOGENOMi HOG000234399.
HOVERGENi HBG076981.
InParanoidi P29452.
KOi K01370.
OMAi KSAEIYP.
OrthoDBi EOG7FXZZ6.
PhylomeDBi P29452.
TreeFami TF102023.

Enzyme and pathway databases

BRENDAi 3.4.22.36. 3474.
Reactomei REACT_198662. The AIM2 inflammasome.
REACT_198700. Interleukin-1 processing.

Miscellaneous databases

NextBioi 281032.
PROi P29452.
SOURCEi Search...

Gene expression databases

Bgeei P29452.
CleanExi MM_CASP1.
Genevestigatori P29452.

Family and domain databases

Gene3Di 1.10.533.10. 1 hit.
3.40.50.1460. 1 hit.
InterProi IPR001315. CARD.
IPR029030. Caspase-like_dom.
IPR017350. Caspase_ICE-type.
IPR011029. DEATH-like_dom.
IPR011600. Pept_C14_caspase.
IPR001309. Pept_C14_ICE_p20.
IPR016129. Pept_C14_ICE_p20_AS.
IPR002138. Pept_C14_p10.
IPR015917. Pept_C14A_p45_core.
[Graphical view ]
Pfami PF00619. CARD. 1 hit.
PF00656. Peptidase_C14. 1 hit.
[Graphical view ]
PIRSFi PIRSF038001. Caspase_ICE. 1 hit.
PRINTSi PR00376. IL1BCENZYME.
SMARTi SM00114. CARD. 1 hit.
SM00115. CASc. 1 hit.
[Graphical view ]
SUPFAMi SSF47986. SSF47986. 1 hit.
PROSITEi PS50209. CARD. 1 hit.
PS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the murine IL-1 beta converting enzyme cDNA."
    Nett-Fiordalisi M.A., Cerretti D.P., Berson D.R., Gilbert D.J., Jenkins N.A., Copeland N.G., Black R.A., Chaplin D.D.
    J. Immunol. 149:3254-3259(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Interleukin 1 beta (IL-1 beta) processing in murine macrophages requires a structurally conserved homologue of human IL-1 beta converting enzyme."
    Molineaux S.M., Casano F.J., Rolando A.M., Peterson E.P., Limjuco G., Chin J., Griffin P.R., Calaycay J.R., Ding G.J.-F., Yamin T.-T., Palyha O.C., Luell S., Fletcher D., Miller D.K., Howard A.D., Thornberry N.A., Kostura M.J.
    Proc. Natl. Acad. Sci. U.S.A. 90:1809-1813(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The structure and complete nucleotide sequence of the murine gene encoding interleukin-1 beta converting enzyme (ICE)."
    Casano F.J., Rolando A.M., Mudgett J.S., Molineaux S.M.
    Genomics 20:474-481(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. Cited for: CHARACTERIZATION.
    Strain: C3H/An.

Entry informationi

Entry nameiCASP1_MOUSE
AccessioniPrimary (citable) accession number: P29452
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3