P29439 (DPO3B_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit beta
EC=2.7.7.7

Gene names

Name:	dnaN
Ordered Locus Names:	BUsg_011

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Contains a core (composed of alpha, epsilon, and theta chains) that can repair short gaps created by nuclease in duplex DNA. For efficient replication of the long, single-stranded templates, pol III requires the auxiliary chains beta, gamma, and delta By similarity.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	DNA polymerase III subunit beta		PRO_0000105427

Sequences

	Sequence		Length	Mass (Da)
	P29439 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 9432FAB06CEFFDF1	FASTA	366	42,180
	10 20 30 40 50 60 MKFTIQNDIL TKNLKKITRV LVKNISFPIL ENILIQVEDG TLSLTTTNLE IELISKIEII 70 80 90 100 110 120 TKYIPGKTTI SGRKILNICR TLSEKSKIKM QLKNKKMYIS SENSNYILST LSADTFPNHQ 130 140 150 160 170 180 NFDYISKFDI SSNILKEMIE KTEFSMGKQD VRYYLNGMLL EKKDKFLRSV ATDGYRLAIS 190 200 210 220 230 240 YTQLKKDINF FSIIIPNKAV MELLKLLNTQ PQLLNILIGS NSIRIYTKNL IFTTQLIEGE 250 260 270 280 290 300 YPDYKSVLFK EKKNPIITNS ILLKKSLLRV AILAHEKFCG IEIKIENGKF KVLSDNQEEE 310 320 330 340 350 360 TAEDLFEIDY FGEKIEISIN VYYLLDVINN IKSENIALFL NKSKSSIQIE AENNSSNAYV VMLLKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of an aphid endosymbiont DNA fragment homologous to the rnpA-rpmH-dnaA-dnaN-gyrB region of eubacteria." Lai C.-Y., Baumann P. Gene 113:175-181(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 36:158-163(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80817 Genomic DNA. Translation: AAA73150.1. AF008210 Genomic DNA. Translation: AAC38107.1. AE013218 Genomic DNA. Translation: AAM67583.1.
PIR	JC1159.
RefSeq	NP_660372.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P29439.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg011.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67583; AAM67583; BUsg_011.
GeneID	1005826.
KEGG	bas:BUsg011.
PATRIC	21246777. VBIBucAph100086_0011.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0592.
KO	K02338.
OMA	DYNRVIP.
ProtClustDB	PRK05643.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-84-MONOMER.
Family and domain databases
InterPro	IPR001001. DNA_polIII_beta. IPR022635. DNA_polIII_beta_C. IPR022637. DNA_polIII_beta_cen. IPR022634. DNA_polIII_beta_N. [Graphical view]
Pfam	PF00712. DNA_pol3_beta. 1 hit. PF02767. DNA_pol3_beta_2. 1 hit. PF02768. DNA_pol3_beta_3. 1 hit. [Graphical view]
PIRSF	PIRSF000804. DNA_pol_III_b. 1 hit.
SMART	SM00480. POL3Bc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00663. dnan. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3B_BUCAP

Accession

Primary (citable) accession number: P29439

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program